Plant growth regulator-free propagation of Hedeoma multiflora Benth: comparison of morphological and volatile metabolomic aspects of in vitro vs in vivo plants

Autores
Diaz Gabutti, Maria Soledad; Magallanes Noguera, Cynthia Alejandra; Cecati, Francisco Miguel; Ferrari, Monica Marta; Leporati, Jorge Leandro; Verdes, Patricia Estela; Kurina Sanz, Marcela Beatriz
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aromatic plant Hedeoma multiflora Benth. is endangered by the overexploitation and the advance of agricultural and urban borders. The objective of this work was to establish an in vitro multiplication protocol for the species without using plant growth regulators (PGRs) and to compare both the morphology and the ability to produce volatile metabolites of the in vitro plants toward wild populations. We were successful in establishing two in vitro plant lines, one initiated from seeds and other from binodal segments. Neither the addition of 1-naphthylacetic acid, kinetin or their combinations improved either growth or multiplication rates which reached 26.4 stems per initial explant after 12 weeks. A comparison toward three wild populations, harvested at both early and late summer time, showed that the morpho-anatomical characteristics of the aerial parts were fairly preserved although in vitro plants displayed more abundant but shorter roots. The profiles of volatile compounds from in vivo and in vitro specimens were quite similar, although hydrocarbon monoterpenes’ fraction was more important in the latter. Pulegone was by far the main volatile metabolite in all the assessed populations while its proportion dropped in the in vitro and in the in vivo plants from late summer with respect to in vivo plants from December. The concentration of the biomarkers, menthone, isomenthone, pulegone and isopulegone, was approximately an order of magnitude lower in the in vitro than in the in vivo plants. Overall, the in vitro biomass production in a PGR-free medium is an appropriate technology for reducing the overexploitation of wild endangered populations and avoiding health safety issues related to the edible consumption of the species.
Fil: Diaz Gabutti, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Magallanes Noguera, Cynthia Alejandra. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: Cecati, Francisco Miguel. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: Ferrari, Monica Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina. Universidad Nacional de San Luis; Argentina
Fil: Leporati, Jorge Leandro. Universidad Nacional de San Luis; Argentina
Fil: Verdes, Patricia Estela. Universidad Nacional de San Luis; Argentina
Fil: Kurina Sanz, Marcela Beatriz. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
Materia
BVOC
HEDEOMA MULTIFLORA BENTH
IN VITRO ORGANOGENESIS
MONOTERPENES
VOLATILOME
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/227387
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/227387
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Plant growth regulator-free propagation of Hedeoma multiflora Benth: comparison of morphological and volatile metabolomic aspects of in vitro vs in vivo plantsDiaz Gabutti, Maria SoledadMagallanes Noguera, Cynthia AlejandraCecati, Francisco MiguelFerrari, Monica MartaLeporati, Jorge LeandroVerdes, Patricia EstelaKurina Sanz, Marcela BeatrizBVOCHEDEOMA MULTIFLORA BENTHIN VITRO ORGANOGENESISMONOTERPENESVOLATILOMEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aromatic plant Hedeoma multiflora Benth. is endangered by the overexploitation and the advance of agricultural and urban borders. The objective of this work was to establish an in vitro multiplication protocol for the species without using plant growth regulators (PGRs) and to compare both the morphology and the ability to produce volatile metabolites of the in vitro plants toward wild populations. We were successful in establishing two in vitro plant lines, one initiated from seeds and other from binodal segments. Neither the addition of 1-naphthylacetic acid, kinetin or their combinations improved either growth or multiplication rates which reached 26.4 stems per initial explant after 12 weeks. A comparison toward three wild populations, harvested at both early and late summer time, showed that the morpho-anatomical characteristics of the aerial parts were fairly preserved although in vitro plants displayed more abundant but shorter roots. The profiles of volatile compounds from in vivo and in vitro specimens were quite similar, although hydrocarbon monoterpenes’ fraction was more important in the latter. Pulegone was by far the main volatile metabolite in all the assessed populations while its proportion dropped in the in vitro and in the in vivo plants from late summer with respect to in vivo plants from December. The concentration of the biomarkers, menthone, isomenthone, pulegone and isopulegone, was approximately an order of magnitude lower in the in vitro than in the in vivo plants. Overall, the in vitro biomass production in a PGR-free medium is an appropriate technology for reducing the overexploitation of wild endangered populations and avoiding health safety issues related to the edible consumption of the species.Fil: Diaz Gabutti, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Magallanes Noguera, Cynthia Alejandra. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; ArgentinaFil: Cecati, Francisco Miguel. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; ArgentinaFil: Ferrari, Monica Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina. Universidad Nacional de San Luis; ArgentinaFil: Leporati, Jorge Leandro. Universidad Nacional de San Luis; ArgentinaFil: Verdes, Patricia Estela. Universidad Nacional de San Luis; ArgentinaFil: Kurina Sanz, Marcela Beatriz. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; ArgentinaSpringer2023-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/227387Diaz Gabutti, Maria Soledad; Magallanes Noguera, Cynthia Alejandra; Cecati, Francisco Miguel; Ferrari, Monica Marta; Leporati, Jorge Leandro; et al.; Plant growth regulator-free propagation of Hedeoma multiflora Benth: comparison of morphological and volatile metabolomic aspects of in vitro vs in vivo plants; Springer; Plant Cell, Tissue and Organ Culture; 153; 3; 3-2023; 587-5980167-6857CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11240-023-02496-5info:eu-repo/semantics/altIdentifier/doi/10.1007/s11240-023-02496-5info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:53:54Zoai:ri.conicet.gov.ar:11336/227387instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:53:55.086CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Plant growth regulator-free propagation of Hedeoma multiflora Benth: comparison of morphological and volatile metabolomic aspects of in vitro vs in vivo plants
title Plant growth regulator-free propagation of Hedeoma multiflora Benth: comparison of morphological and volatile metabolomic aspects of in vitro vs in vivo plants
spellingShingle Plant growth regulator-free propagation of Hedeoma multiflora Benth: comparison of morphological and volatile metabolomic aspects of in vitro vs in vivo plants
Diaz Gabutti, Maria Soledad
BVOC
HEDEOMA MULTIFLORA BENTH
IN VITRO ORGANOGENESIS
MONOTERPENES
VOLATILOME
title_short Plant growth regulator-free propagation of Hedeoma multiflora Benth: comparison of morphological and volatile metabolomic aspects of in vitro vs in vivo plants
title_full Plant growth regulator-free propagation of Hedeoma multiflora Benth: comparison of morphological and volatile metabolomic aspects of in vitro vs in vivo plants
title_fullStr Plant growth regulator-free propagation of Hedeoma multiflora Benth: comparison of morphological and volatile metabolomic aspects of in vitro vs in vivo plants
title_full_unstemmed Plant growth regulator-free propagation of Hedeoma multiflora Benth: comparison of morphological and volatile metabolomic aspects of in vitro vs in vivo plants
title_sort Plant growth regulator-free propagation of Hedeoma multiflora Benth: comparison of morphological and volatile metabolomic aspects of in vitro vs in vivo plants
dc.creator.none.fl_str_mv Diaz Gabutti, Maria Soledad
Magallanes Noguera, Cynthia Alejandra
Cecati, Francisco Miguel
Ferrari, Monica Marta
Leporati, Jorge Leandro
Verdes, Patricia Estela
Kurina Sanz, Marcela Beatriz
author Diaz Gabutti, Maria Soledad
author_facet Diaz Gabutti, Maria Soledad
Magallanes Noguera, Cynthia Alejandra
Cecati, Francisco Miguel
Ferrari, Monica Marta
Leporati, Jorge Leandro
Verdes, Patricia Estela
Kurina Sanz, Marcela Beatriz
author_role author
author2 Magallanes Noguera, Cynthia Alejandra
Cecati, Francisco Miguel
Ferrari, Monica Marta
Leporati, Jorge Leandro
Verdes, Patricia Estela
Kurina Sanz, Marcela Beatriz
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BVOC
HEDEOMA MULTIFLORA BENTH
IN VITRO ORGANOGENESIS
MONOTERPENES
VOLATILOME
topic BVOC
HEDEOMA MULTIFLORA BENTH
IN VITRO ORGANOGENESIS
MONOTERPENES
VOLATILOME
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aromatic plant Hedeoma multiflora Benth. is endangered by the overexploitation and the advance of agricultural and urban borders. The objective of this work was to establish an in vitro multiplication protocol for the species without using plant growth regulators (PGRs) and to compare both the morphology and the ability to produce volatile metabolites of the in vitro plants toward wild populations. We were successful in establishing two in vitro plant lines, one initiated from seeds and other from binodal segments. Neither the addition of 1-naphthylacetic acid, kinetin or their combinations improved either growth or multiplication rates which reached 26.4 stems per initial explant after 12 weeks. A comparison toward three wild populations, harvested at both early and late summer time, showed that the morpho-anatomical characteristics of the aerial parts were fairly preserved although in vitro plants displayed more abundant but shorter roots. The profiles of volatile compounds from in vivo and in vitro specimens were quite similar, although hydrocarbon monoterpenes’ fraction was more important in the latter. Pulegone was by far the main volatile metabolite in all the assessed populations while its proportion dropped in the in vitro and in the in vivo plants from late summer with respect to in vivo plants from December. The concentration of the biomarkers, menthone, isomenthone, pulegone and isopulegone, was approximately an order of magnitude lower in the in vitro than in the in vivo plants. Overall, the in vitro biomass production in a PGR-free medium is an appropriate technology for reducing the overexploitation of wild endangered populations and avoiding health safety issues related to the edible consumption of the species.
Fil: Diaz Gabutti, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Magallanes Noguera, Cynthia Alejandra. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: Cecati, Francisco Miguel. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: Ferrari, Monica Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina. Universidad Nacional de San Luis; Argentina
Fil: Leporati, Jorge Leandro. Universidad Nacional de San Luis; Argentina
Fil: Verdes, Patricia Estela. Universidad Nacional de San Luis; Argentina
Fil: Kurina Sanz, Marcela Beatriz. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
description The aromatic plant Hedeoma multiflora Benth. is endangered by the overexploitation and the advance of agricultural and urban borders. The objective of this work was to establish an in vitro multiplication protocol for the species without using plant growth regulators (PGRs) and to compare both the morphology and the ability to produce volatile metabolites of the in vitro plants toward wild populations. We were successful in establishing two in vitro plant lines, one initiated from seeds and other from binodal segments. Neither the addition of 1-naphthylacetic acid, kinetin or their combinations improved either growth or multiplication rates which reached 26.4 stems per initial explant after 12 weeks. A comparison toward three wild populations, harvested at both early and late summer time, showed that the morpho-anatomical characteristics of the aerial parts were fairly preserved although in vitro plants displayed more abundant but shorter roots. The profiles of volatile compounds from in vivo and in vitro specimens were quite similar, although hydrocarbon monoterpenes’ fraction was more important in the latter. Pulegone was by far the main volatile metabolite in all the assessed populations while its proportion dropped in the in vitro and in the in vivo plants from late summer with respect to in vivo plants from December. The concentration of the biomarkers, menthone, isomenthone, pulegone and isopulegone, was approximately an order of magnitude lower in the in vitro than in the in vivo plants. Overall, the in vitro biomass production in a PGR-free medium is an appropriate technology for reducing the overexploitation of wild endangered populations and avoiding health safety issues related to the edible consumption of the species.
publishDate 2023
dc.date.none.fl_str_mv 2023-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/227387
Diaz Gabutti, Maria Soledad; Magallanes Noguera, Cynthia Alejandra; Cecati, Francisco Miguel; Ferrari, Monica Marta; Leporati, Jorge Leandro; et al.; Plant growth regulator-free propagation of Hedeoma multiflora Benth: comparison of morphological and volatile metabolomic aspects of in vitro vs in vivo plants; Springer; Plant Cell, Tissue and Organ Culture; 153; 3; 3-2023; 587-598
0167-6857
CONICET Digital
CONICET
url http://hdl.handle.net/11336/227387
identifier_str_mv Diaz Gabutti, Maria Soledad; Magallanes Noguera, Cynthia Alejandra; Cecati, Francisco Miguel; Ferrari, Monica Marta; Leporati, Jorge Leandro; et al.; Plant growth regulator-free propagation of Hedeoma multiflora Benth: comparison of morphological and volatile metabolomic aspects of in vitro vs in vivo plants; Springer; Plant Cell, Tissue and Organ Culture; 153; 3; 3-2023; 587-598
0167-6857
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1007/s11240-023-02496-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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