Simultaneous expression analysis of DAZ-family genes and CDC25A: their potential as a predictor for successful TESE

Autores
Gonzalez, Candela Rocio; Alvarez Sedó, Cristian; Nodar, Florencia; Papier, Sergio; Vitullo, Alfredo Daniel
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The analysis of single DAZ genes has shown that their dysfunction leads to abnormal spermatogenesis and may cause infertility. They were, however, poorly associated to sperm recovery during assisted reproductive treatments. Whether the simultaneous expression of DAZ genes and its substrates may provide better information of testicular damage and success of sperm recovery in infertile patients has not been yet analyzed. We evaluated eight 8 men (29-38 years-old) presenting idiopathic infertility and non-obstructive azoospermia without infection processes diagnosed by open testicular biopsy. None of these patients presented showed genitourinary infections, varicocele, hypogonadotropic hypogonadism, chromosome anomalies abnormalities and obstruction or agenesis of the seminal ducts.We did not observe find an association between the expression levels of DAZ genes family members analyzed by real time PCR with the diagnosis of the testicular pathology. However, a positive correlation between the mRNA transcript ratios of DAZ gene family members and CDC25A were was detected, irrespective of the testicular pathology. Moreover, we found that, irrespective of the testicular pathology, the group of patients with failure in sperm retrieval showed a significantly lower expression levels of DAZ, BOULE and CDC25A compared to the group of patients with successful sperm retrieval.These results suggest that DAZ genes would be collectively rather than individually altered in patients with HS and MA.
Fil: Gonzalez, Candela Rocio. Universidad Maimónides; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alvarez Sedó, Cristian. Centro de Estudios en Ginecología y Reproducción; Argentina
Fil: Nodar, Florencia. Centro de Estudios en Ginecología y Reproducción; Argentina
Fil: Papier, Sergio. Centro de Estudios en Ginecología y Reproducción; Argentina
Fil: Vitullo, Alfredo Daniel. Universidad Maimónides; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Daz Genes
Cdc25a
Hypospermatogenesis
Maturation Arrest
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/46471
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/46471
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Simultaneous expression analysis of DAZ-family genes and CDC25A: their potential as a predictor for successful TESEGonzalez, Candela RocioAlvarez Sedó, CristianNodar, FlorenciaPapier, SergioVitullo, Alfredo DanielDaz GenesCdc25aHypospermatogenesisMaturation Arresthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The analysis of single DAZ genes has shown that their dysfunction leads to abnormal spermatogenesis and may cause infertility. They were, however, poorly associated to sperm recovery during assisted reproductive treatments. Whether the simultaneous expression of DAZ genes and its substrates may provide better information of testicular damage and success of sperm recovery in infertile patients has not been yet analyzed. We evaluated eight 8 men (29-38 years-old) presenting idiopathic infertility and non-obstructive azoospermia without infection processes diagnosed by open testicular biopsy. None of these patients presented showed genitourinary infections, varicocele, hypogonadotropic hypogonadism, chromosome anomalies abnormalities and obstruction or agenesis of the seminal ducts.We did not observe find an association between the expression levels of DAZ genes family members analyzed by real time PCR with the diagnosis of the testicular pathology. However, a positive correlation between the mRNA transcript ratios of DAZ gene family members and CDC25A were was detected, irrespective of the testicular pathology. Moreover, we found that, irrespective of the testicular pathology, the group of patients with failure in sperm retrieval showed a significantly lower expression levels of DAZ, BOULE and CDC25A compared to the group of patients with successful sperm retrieval.These results suggest that DAZ genes would be collectively rather than individually altered in patients with HS and MA.Fil: Gonzalez, Candela Rocio. Universidad Maimónides; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alvarez Sedó, Cristian. Centro de Estudios en Ginecología y Reproducción; ArgentinaFil: Nodar, Florencia. Centro de Estudios en Ginecología y Reproducción; ArgentinaFil: Papier, Sergio. Centro de Estudios en Ginecología y Reproducción; ArgentinaFil: Vitullo, Alfredo Daniel. Universidad Maimónides; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaAsian Society of Andrology2017-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/46471Gonzalez, Candela Rocio; Alvarez Sedó, Cristian; Nodar, Florencia; Papier, Sergio; Vitullo, Alfredo Daniel; Simultaneous expression analysis of DAZ-family genes and CDC25A: their potential as a predictor for successful TESE; Asian Society of Andrology; Asian Journal of Andrology; 19; 6; 11-2017; 715-7161008-682XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4103/1008-682X.184993info:eu-repo/semantics/altIdentifier/url/http://www.ajandrology.com/article.asp?issn=1008-682X;year=2017;volume=19;issue=6;spage=715;epage=716;aulast=Gonz%E1lezinfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5676433/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:58Zoai:ri.conicet.gov.ar:11336/46471instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:59.106CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Simultaneous expression analysis of DAZ-family genes and CDC25A: their potential as a predictor for successful TESE
title Simultaneous expression analysis of DAZ-family genes and CDC25A: their potential as a predictor for successful TESE
spellingShingle Simultaneous expression analysis of DAZ-family genes and CDC25A: their potential as a predictor for successful TESE
Gonzalez, Candela Rocio
Daz Genes
Cdc25a
Hypospermatogenesis
Maturation Arrest
title_short Simultaneous expression analysis of DAZ-family genes and CDC25A: their potential as a predictor for successful TESE
title_full Simultaneous expression analysis of DAZ-family genes and CDC25A: their potential as a predictor for successful TESE
title_fullStr Simultaneous expression analysis of DAZ-family genes and CDC25A: their potential as a predictor for successful TESE
title_full_unstemmed Simultaneous expression analysis of DAZ-family genes and CDC25A: their potential as a predictor for successful TESE
title_sort Simultaneous expression analysis of DAZ-family genes and CDC25A: their potential as a predictor for successful TESE
dc.creator.none.fl_str_mv Gonzalez, Candela Rocio
Alvarez Sedó, Cristian
Nodar, Florencia
Papier, Sergio
Vitullo, Alfredo Daniel
author Gonzalez, Candela Rocio
author_facet Gonzalez, Candela Rocio
Alvarez Sedó, Cristian
Nodar, Florencia
Papier, Sergio
Vitullo, Alfredo Daniel
author_role author
author2 Alvarez Sedó, Cristian
Nodar, Florencia
Papier, Sergio
Vitullo, Alfredo Daniel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Daz Genes
Cdc25a
Hypospermatogenesis
Maturation Arrest
topic Daz Genes
Cdc25a
Hypospermatogenesis
Maturation Arrest
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The analysis of single DAZ genes has shown that their dysfunction leads to abnormal spermatogenesis and may cause infertility. They were, however, poorly associated to sperm recovery during assisted reproductive treatments. Whether the simultaneous expression of DAZ genes and its substrates may provide better information of testicular damage and success of sperm recovery in infertile patients has not been yet analyzed. We evaluated eight 8 men (29-38 years-old) presenting idiopathic infertility and non-obstructive azoospermia without infection processes diagnosed by open testicular biopsy. None of these patients presented showed genitourinary infections, varicocele, hypogonadotropic hypogonadism, chromosome anomalies abnormalities and obstruction or agenesis of the seminal ducts.We did not observe find an association between the expression levels of DAZ genes family members analyzed by real time PCR with the diagnosis of the testicular pathology. However, a positive correlation between the mRNA transcript ratios of DAZ gene family members and CDC25A were was detected, irrespective of the testicular pathology. Moreover, we found that, irrespective of the testicular pathology, the group of patients with failure in sperm retrieval showed a significantly lower expression levels of DAZ, BOULE and CDC25A compared to the group of patients with successful sperm retrieval.These results suggest that DAZ genes would be collectively rather than individually altered in patients with HS and MA.
Fil: Gonzalez, Candela Rocio. Universidad Maimónides; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alvarez Sedó, Cristian. Centro de Estudios en Ginecología y Reproducción; Argentina
Fil: Nodar, Florencia. Centro de Estudios en Ginecología y Reproducción; Argentina
Fil: Papier, Sergio. Centro de Estudios en Ginecología y Reproducción; Argentina
Fil: Vitullo, Alfredo Daniel. Universidad Maimónides; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The analysis of single DAZ genes has shown that their dysfunction leads to abnormal spermatogenesis and may cause infertility. They were, however, poorly associated to sperm recovery during assisted reproductive treatments. Whether the simultaneous expression of DAZ genes and its substrates may provide better information of testicular damage and success of sperm recovery in infertile patients has not been yet analyzed. We evaluated eight 8 men (29-38 years-old) presenting idiopathic infertility and non-obstructive azoospermia without infection processes diagnosed by open testicular biopsy. None of these patients presented showed genitourinary infections, varicocele, hypogonadotropic hypogonadism, chromosome anomalies abnormalities and obstruction or agenesis of the seminal ducts.We did not observe find an association between the expression levels of DAZ genes family members analyzed by real time PCR with the diagnosis of the testicular pathology. However, a positive correlation between the mRNA transcript ratios of DAZ gene family members and CDC25A were was detected, irrespective of the testicular pathology. Moreover, we found that, irrespective of the testicular pathology, the group of patients with failure in sperm retrieval showed a significantly lower expression levels of DAZ, BOULE and CDC25A compared to the group of patients with successful sperm retrieval.These results suggest that DAZ genes would be collectively rather than individually altered in patients with HS and MA.
publishDate 2017
dc.date.none.fl_str_mv 2017-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/46471
Gonzalez, Candela Rocio; Alvarez Sedó, Cristian; Nodar, Florencia; Papier, Sergio; Vitullo, Alfredo Daniel; Simultaneous expression analysis of DAZ-family genes and CDC25A: their potential as a predictor for successful TESE; Asian Society of Andrology; Asian Journal of Andrology; 19; 6; 11-2017; 715-716
1008-682X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/46471
identifier_str_mv Gonzalez, Candela Rocio; Alvarez Sedó, Cristian; Nodar, Florencia; Papier, Sergio; Vitullo, Alfredo Daniel; Simultaneous expression analysis of DAZ-family genes and CDC25A: their potential as a predictor for successful TESE; Asian Society of Andrology; Asian Journal of Andrology; 19; 6; 11-2017; 715-716
1008-682X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4103/1008-682X.184993
info:eu-repo/semantics/altIdentifier/url/http://www.ajandrology.com/article.asp?issn=1008-682X;year=2017;volume=19;issue=6;spage=715;epage=716;aulast=Gonz%E1lez
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5676433/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Asian Society of Andrology
publisher.none.fl_str_mv Asian Society of Andrology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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