Immobilization of enzymes into self-assembled iron(III) hydrous oxide nano-scaffolds: A bio-inspired one-pot approach to hybrid catalysts

Autores
Bellino, Martin Gonzalo; Regazzoni, Alberto Ernesto
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A simple bio-inspired one-pot procedure for the immobilization of α-amylase into maturing hybrid iron(III) hydrous oxide nanostructures is described. The method resorts to the urease mediated decomposition of urea to induce the homogeneous precipitation of amylase-iron(III) hydrous oxide ensembles. Appropriate setting of the synthesis parameters, which control the shape and texture of the resulting hybrid nanostructures, is key to amylase entrapment. Highly efficient hybrid catalysts were prepared at the lowest urease concentration (0.5 mg/mL), where spherical 100 nm size hybrid iron(III) hydrous oxide ensembles formed; their amylase load depended on the enzyme concentration, in a michaelian fashion. Their specific activity is nearly that of free amylase. These catalysts are reusable, with no loss of performance, and substantially more active than the free enzyme at extreme pHs and temperatures. The high efficiency of the hybrid ensembles is ascribed to their open structure, high enzyme loading, and negligible amylase inactivation.
Fil: Bellino, Martin Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes; Argentina
Fil: Regazzoni, Alberto Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes; Argentina. Universidad Nacional de San Martín. Instituto Sabato; Argentina
Materia
Α-AMYLASE
BIOCATALYSTS
HYBRID NANOPARTICLES
IMMOBILIZATION
IRON (HYDROUS)OXIDE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/194136
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immobilization of enzymes into self-assembled iron(III) hydrous oxide nano-scaffolds: A bio-inspired one-pot approach to hybrid catalystsBellino, Martin GonzaloRegazzoni, Alberto ErnestoΑ-AMYLASEBIOCATALYSTSHYBRID NANOPARTICLESIMMOBILIZATIONIRON (HYDROUS)OXIDEhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2https://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2A simple bio-inspired one-pot procedure for the immobilization of α-amylase into maturing hybrid iron(III) hydrous oxide nanostructures is described. The method resorts to the urease mediated decomposition of urea to induce the homogeneous precipitation of amylase-iron(III) hydrous oxide ensembles. Appropriate setting of the synthesis parameters, which control the shape and texture of the resulting hybrid nanostructures, is key to amylase entrapment. Highly efficient hybrid catalysts were prepared at the lowest urease concentration (0.5 mg/mL), where spherical 100 nm size hybrid iron(III) hydrous oxide ensembles formed; their amylase load depended on the enzyme concentration, in a michaelian fashion. Their specific activity is nearly that of free amylase. These catalysts are reusable, with no loss of performance, and substantially more active than the free enzyme at extreme pHs and temperatures. The high efficiency of the hybrid ensembles is ascribed to their open structure, high enzyme loading, and negligible amylase inactivation.Fil: Bellino, Martin Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes; ArgentinaFil: Regazzoni, Alberto Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes; Argentina. Universidad Nacional de San Martín. Instituto Sabato; ArgentinaElsevier Science2011-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/194136Bellino, Martin Gonzalo; Regazzoni, Alberto Ernesto; Immobilization of enzymes into self-assembled iron(III) hydrous oxide nano-scaffolds: A bio-inspired one-pot approach to hybrid catalysts; Elsevier Science; Applied Catalysis A: General; 408; 1-2; 11-2011; 73-770926-860XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0926860X11005333info:eu-repo/semantics/altIdentifier/doi/10.1016/j.apcata.2011.09.008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:53:34Zoai:ri.conicet.gov.ar:11336/194136instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:53:34.656CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immobilization of enzymes into self-assembled iron(III) hydrous oxide nano-scaffolds: A bio-inspired one-pot approach to hybrid catalysts
title Immobilization of enzymes into self-assembled iron(III) hydrous oxide nano-scaffolds: A bio-inspired one-pot approach to hybrid catalysts
spellingShingle Immobilization of enzymes into self-assembled iron(III) hydrous oxide nano-scaffolds: A bio-inspired one-pot approach to hybrid catalysts
Bellino, Martin Gonzalo
Α-AMYLASE
BIOCATALYSTS
HYBRID NANOPARTICLES
IMMOBILIZATION
IRON (HYDROUS)OXIDE
title_short Immobilization of enzymes into self-assembled iron(III) hydrous oxide nano-scaffolds: A bio-inspired one-pot approach to hybrid catalysts
title_full Immobilization of enzymes into self-assembled iron(III) hydrous oxide nano-scaffolds: A bio-inspired one-pot approach to hybrid catalysts
title_fullStr Immobilization of enzymes into self-assembled iron(III) hydrous oxide nano-scaffolds: A bio-inspired one-pot approach to hybrid catalysts
title_full_unstemmed Immobilization of enzymes into self-assembled iron(III) hydrous oxide nano-scaffolds: A bio-inspired one-pot approach to hybrid catalysts
title_sort Immobilization of enzymes into self-assembled iron(III) hydrous oxide nano-scaffolds: A bio-inspired one-pot approach to hybrid catalysts
dc.creator.none.fl_str_mv Bellino, Martin Gonzalo
Regazzoni, Alberto Ernesto
author Bellino, Martin Gonzalo
author_facet Bellino, Martin Gonzalo
Regazzoni, Alberto Ernesto
author_role author
author2 Regazzoni, Alberto Ernesto
author2_role author
dc.subject.none.fl_str_mv Α-AMYLASE
BIOCATALYSTS
HYBRID NANOPARTICLES
IMMOBILIZATION
IRON (HYDROUS)OXIDE
topic Α-AMYLASE
BIOCATALYSTS
HYBRID NANOPARTICLES
IMMOBILIZATION
IRON (HYDROUS)OXIDE
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv A simple bio-inspired one-pot procedure for the immobilization of α-amylase into maturing hybrid iron(III) hydrous oxide nanostructures is described. The method resorts to the urease mediated decomposition of urea to induce the homogeneous precipitation of amylase-iron(III) hydrous oxide ensembles. Appropriate setting of the synthesis parameters, which control the shape and texture of the resulting hybrid nanostructures, is key to amylase entrapment. Highly efficient hybrid catalysts were prepared at the lowest urease concentration (0.5 mg/mL), where spherical 100 nm size hybrid iron(III) hydrous oxide ensembles formed; their amylase load depended on the enzyme concentration, in a michaelian fashion. Their specific activity is nearly that of free amylase. These catalysts are reusable, with no loss of performance, and substantially more active than the free enzyme at extreme pHs and temperatures. The high efficiency of the hybrid ensembles is ascribed to their open structure, high enzyme loading, and negligible amylase inactivation.
Fil: Bellino, Martin Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes; Argentina
Fil: Regazzoni, Alberto Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes; Argentina. Universidad Nacional de San Martín. Instituto Sabato; Argentina
description A simple bio-inspired one-pot procedure for the immobilization of α-amylase into maturing hybrid iron(III) hydrous oxide nanostructures is described. The method resorts to the urease mediated decomposition of urea to induce the homogeneous precipitation of amylase-iron(III) hydrous oxide ensembles. Appropriate setting of the synthesis parameters, which control the shape and texture of the resulting hybrid nanostructures, is key to amylase entrapment. Highly efficient hybrid catalysts were prepared at the lowest urease concentration (0.5 mg/mL), where spherical 100 nm size hybrid iron(III) hydrous oxide ensembles formed; their amylase load depended on the enzyme concentration, in a michaelian fashion. Their specific activity is nearly that of free amylase. These catalysts are reusable, with no loss of performance, and substantially more active than the free enzyme at extreme pHs and temperatures. The high efficiency of the hybrid ensembles is ascribed to their open structure, high enzyme loading, and negligible amylase inactivation.
publishDate 2011
dc.date.none.fl_str_mv 2011-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/194136
Bellino, Martin Gonzalo; Regazzoni, Alberto Ernesto; Immobilization of enzymes into self-assembled iron(III) hydrous oxide nano-scaffolds: A bio-inspired one-pot approach to hybrid catalysts; Elsevier Science; Applied Catalysis A: General; 408; 1-2; 11-2011; 73-77
0926-860X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/194136
identifier_str_mv Bellino, Martin Gonzalo; Regazzoni, Alberto Ernesto; Immobilization of enzymes into self-assembled iron(III) hydrous oxide nano-scaffolds: A bio-inspired one-pot approach to hybrid catalysts; Elsevier Science; Applied Catalysis A: General; 408; 1-2; 11-2011; 73-77
0926-860X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0926860X11005333
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.apcata.2011.09.008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613635234594816
score 13.070432

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