Immobilization of catalase from Aspergillus niger on inorganic and biopolymeric supports for H2O2 decomposition

Autores
Eberhardt, Andrea Mariel; Pedroni, Viviana Isabel; Volpe, María Alicia; Ferreira, María Luján
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This paper studies the H2O2 decomposition using supported catalase. The supports are γ-Al2O3, FeCl2-treated γ-Al2O3, glutaraldehyde-treated chitosan and glutaraldehyde-treated cellulose. Catalase/γ-Al2O3 is inactive, but the treatment with FeCl2 renders the oxide active. The extended Hückel type analysis (EHMO) indicates that the alumina-catalase interaction is strong, suggesting that a denaturation of the enzyme is accomplished when supporting on this oxide. Amongst all the samples, catalase/glutaraldehyde-cellulose is the most active, especially after 6 months of storage. After aging, catalase/glutaraldehyde-chitosan becomes inactive, probably due to the fact that NH2 groups on chitosan react with the enzyme active sites.
Fil: Eberhardt, Andrea Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Fil: Pedroni, Viviana Isabel. Universidad Nacional del Sur. Departamento de Química; Argentina
Fil: Volpe, María Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Fil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Materia
BIOCATALYSTS
OXIDOREDUCTASES
PEROXIDE DECOMPOSITION
SUPPORTED
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/97823
Acceder
id CONICETDig_cde5607f10401bed4e212657fc4dacac
oai_identifier_str oai:ri.conicet.gov.ar:11336/97823
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immobilization of catalase from Aspergillus niger on inorganic and biopolymeric supports for H2O2 decompositionEberhardt, Andrea MarielPedroni, Viviana IsabelVolpe, María AliciaFerreira, María LujánBIOCATALYSTSOXIDOREDUCTASESPEROXIDE DECOMPOSITIONSUPPORTEDhttps://purl.org/becyt/ford/2.4https://purl.org/becyt/ford/2This paper studies the H2O2 decomposition using supported catalase. The supports are γ-Al2O3, FeCl2-treated γ-Al2O3, glutaraldehyde-treated chitosan and glutaraldehyde-treated cellulose. Catalase/γ-Al2O3 is inactive, but the treatment with FeCl2 renders the oxide active. The extended Hückel type analysis (EHMO) indicates that the alumina-catalase interaction is strong, suggesting that a denaturation of the enzyme is accomplished when supporting on this oxide. Amongst all the samples, catalase/glutaraldehyde-cellulose is the most active, especially after 6 months of storage. After aging, catalase/glutaraldehyde-chitosan becomes inactive, probably due to the fact that NH2 groups on chitosan react with the enzyme active sites.Fil: Eberhardt, Andrea Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; ArgentinaFil: Pedroni, Viviana Isabel. Universidad Nacional del Sur. Departamento de Química; ArgentinaFil: Volpe, María Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; ArgentinaFil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; ArgentinaElsevier Science2004-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/97823Eberhardt, Andrea Mariel; Pedroni, Viviana Isabel; Volpe, María Alicia; Ferreira, María Luján; Immobilization of catalase from Aspergillus niger on inorganic and biopolymeric supports for H2O2 decomposition; Elsevier Science; Applied Catalysis B: Environmental; 47; 3; 2-2004; 153-1630926-3373CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.apcatb.2003.08.007info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0926337303004855info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:22:57Zoai:ri.conicet.gov.ar:11336/97823instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:22:58.256CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immobilization of catalase from Aspergillus niger on inorganic and biopolymeric supports for H2O2 decomposition
title Immobilization of catalase from Aspergillus niger on inorganic and biopolymeric supports for H2O2 decomposition
spellingShingle Immobilization of catalase from Aspergillus niger on inorganic and biopolymeric supports for H2O2 decomposition
Eberhardt, Andrea Mariel
BIOCATALYSTS
OXIDOREDUCTASES
PEROXIDE DECOMPOSITION
SUPPORTED
title_short Immobilization of catalase from Aspergillus niger on inorganic and biopolymeric supports for H2O2 decomposition
title_full Immobilization of catalase from Aspergillus niger on inorganic and biopolymeric supports for H2O2 decomposition
title_fullStr Immobilization of catalase from Aspergillus niger on inorganic and biopolymeric supports for H2O2 decomposition
title_full_unstemmed Immobilization of catalase from Aspergillus niger on inorganic and biopolymeric supports for H2O2 decomposition
title_sort Immobilization of catalase from Aspergillus niger on inorganic and biopolymeric supports for H2O2 decomposition
dc.creator.none.fl_str_mv Eberhardt, Andrea Mariel
Pedroni, Viviana Isabel
Volpe, María Alicia
Ferreira, María Luján
author Eberhardt, Andrea Mariel
author_facet Eberhardt, Andrea Mariel
Pedroni, Viviana Isabel
Volpe, María Alicia
Ferreira, María Luján
author_role author
author2 Pedroni, Viviana Isabel
Volpe, María Alicia
Ferreira, María Luján
author2_role author
author
author
dc.subject.none.fl_str_mv BIOCATALYSTS
OXIDOREDUCTASES
PEROXIDE DECOMPOSITION
SUPPORTED
topic BIOCATALYSTS
OXIDOREDUCTASES
PEROXIDE DECOMPOSITION
SUPPORTED
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.4
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv This paper studies the H2O2 decomposition using supported catalase. The supports are γ-Al2O3, FeCl2-treated γ-Al2O3, glutaraldehyde-treated chitosan and glutaraldehyde-treated cellulose. Catalase/γ-Al2O3 is inactive, but the treatment with FeCl2 renders the oxide active. The extended Hückel type analysis (EHMO) indicates that the alumina-catalase interaction is strong, suggesting that a denaturation of the enzyme is accomplished when supporting on this oxide. Amongst all the samples, catalase/glutaraldehyde-cellulose is the most active, especially after 6 months of storage. After aging, catalase/glutaraldehyde-chitosan becomes inactive, probably due to the fact that NH2 groups on chitosan react with the enzyme active sites.
Fil: Eberhardt, Andrea Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Fil: Pedroni, Viviana Isabel. Universidad Nacional del Sur. Departamento de Química; Argentina
Fil: Volpe, María Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Fil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
description This paper studies the H2O2 decomposition using supported catalase. The supports are γ-Al2O3, FeCl2-treated γ-Al2O3, glutaraldehyde-treated chitosan and glutaraldehyde-treated cellulose. Catalase/γ-Al2O3 is inactive, but the treatment with FeCl2 renders the oxide active. The extended Hückel type analysis (EHMO) indicates that the alumina-catalase interaction is strong, suggesting that a denaturation of the enzyme is accomplished when supporting on this oxide. Amongst all the samples, catalase/glutaraldehyde-cellulose is the most active, especially after 6 months of storage. After aging, catalase/glutaraldehyde-chitosan becomes inactive, probably due to the fact that NH2 groups on chitosan react with the enzyme active sites.
publishDate 2004
dc.date.none.fl_str_mv 2004-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/97823
Eberhardt, Andrea Mariel; Pedroni, Viviana Isabel; Volpe, María Alicia; Ferreira, María Luján; Immobilization of catalase from Aspergillus niger on inorganic and biopolymeric supports for H2O2 decomposition; Elsevier Science; Applied Catalysis B: Environmental; 47; 3; 2-2004; 153-163
0926-3373
CONICET Digital
CONICET
url http://hdl.handle.net/11336/97823
identifier_str_mv Eberhardt, Andrea Mariel; Pedroni, Viviana Isabel; Volpe, María Alicia; Ferreira, María Luján; Immobilization of catalase from Aspergillus niger on inorganic and biopolymeric supports for H2O2 decomposition; Elsevier Science; Applied Catalysis B: Environmental; 47; 3; 2-2004; 153-163
0926-3373
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.apcatb.2003.08.007
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0926337303004855
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781752950915072
score 12.982451

Publicaciones similares