Ovarian Follicular Dynamics and Its Functional Significance in Relation with Follicle Deviation, Vaginal Cytology, and Hormone Profiles in Llamas (Lama glama)

Autores
Perez Guerra, Uri Harold; Quispe, Yesenia M.; Gonzáles, Henry I.; Luque, Natalio; Ruelas, Domingo A.; Carretero, Maria Ignacia; Gutiérrez Reinoso, Miguel A.; Pérez Durand, Manuel G.; García Herreros, Manuel
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The reproductive physiology in camelid species has its particularities. The present study aimed to characterize the ovarian follicular dynamics and its functional significance in relation to follicular deviation, vaginal cytological characteristics, and sexual hormone profiles in llamas as the first report in South American camelids. Non-pregnant, multiparous llamas (Lama glama; n = 10; age: 48–72 mo.; BCS: 2.5–3.0) were enrolled in the study. The ultrasonographic assessment was carried out transvaginally and follicular ablation was performed (day 0) when follicles were larger than 7 mm. The follicle number and diameter were scored daily throughout the process for a proper evaluation of the deviated follicles and to monitor the presence of new follicle pools (1.5 to 2.5 mm diameter). Vaginal cytological evaluation (parabasal, intermediate, and superficial cells) was performed every other day until day 6. Endocrine profiles (17β estradiol, anti-Mullerian hormone, testosterone, and progesterone) during pre- and post-follicular deviation were determined by using the ELISA assay. Differential follicular dynamics both in the presence of a single dominant follicle (DF) and in codominance during the follicular deviation process were detected in llamas (p < 0.05). The percentage of superficial cells was the most related to the follicular wave phase. However, the percentage of parabasal, intermediate, and superficial cells was not related to the phases of follicular growth, dominance, and regression (p > 0.05). Differential patterns among the different hormone concentration levels regarding the 17β estradiol, anti-Mullerian hormone, progesterone, and testosterone during follicular deviation were observed, with the latter being significantly different along the deviation process (p < 0.05). In conclusion, the use of vaginal cytology assessment would not be sufficient to determine the follicular phases in llamas. Therefore, complementary analyses, such as ultrasonography and endocrine assessment, are strongly recommended to determine follicular dynamics during the follicular deviation.
Fil: Perez Guerra, Uri Harold. Universidad Nacional del Altiplano; Perú
Fil: Quispe, Yesenia M.. Universidad Nacional del Altiplano; Perú
Fil: Gonzáles, Henry I.. Universidad Nacional del Altiplano; Perú
Fil: Luque, Natalio. Universidad Nacional del Altiplano; Perú
Fil: Ruelas, Domingo A.. Universidad Nacional del Altiplano; Perú
Fil: Carretero, Maria Ignacia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal; Argentina
Fil: Gutiérrez Reinoso, Miguel A.. Universidad Técnica de Cotopaxi; Ecuador
Fil: Pérez Durand, Manuel G.. Universidad Nacional del Altiplano; Perú
Fil: García Herreros, Manuel. Instituto Nacional de Investigação Agrária e Veterinária; Portugal
Materia
ENDOCRINE PROFILES
FOLLICLE DEVIATION
FOLLICULAR DYNAMICS
LLAMA
VAGINAL CYTOLOGY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/201473
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/201473
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ovarian Follicular Dynamics and Its Functional Significance in Relation with Follicle Deviation, Vaginal Cytology, and Hormone Profiles in Llamas (Lama glama)Perez Guerra, Uri HaroldQuispe, Yesenia M.Gonzáles, Henry I.Luque, NatalioRuelas, Domingo A.Carretero, Maria IgnaciaGutiérrez Reinoso, Miguel A.Pérez Durand, Manuel G.García Herreros, ManuelENDOCRINE PROFILESFOLLICLE DEVIATIONFOLLICULAR DYNAMICSLLAMAVAGINAL CYTOLOGYhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The reproductive physiology in camelid species has its particularities. The present study aimed to characterize the ovarian follicular dynamics and its functional significance in relation to follicular deviation, vaginal cytological characteristics, and sexual hormone profiles in llamas as the first report in South American camelids. Non-pregnant, multiparous llamas (Lama glama; n = 10; age: 48–72 mo.; BCS: 2.5–3.0) were enrolled in the study. The ultrasonographic assessment was carried out transvaginally and follicular ablation was performed (day 0) when follicles were larger than 7 mm. The follicle number and diameter were scored daily throughout the process for a proper evaluation of the deviated follicles and to monitor the presence of new follicle pools (1.5 to 2.5 mm diameter). Vaginal cytological evaluation (parabasal, intermediate, and superficial cells) was performed every other day until day 6. Endocrine profiles (17β estradiol, anti-Mullerian hormone, testosterone, and progesterone) during pre- and post-follicular deviation were determined by using the ELISA assay. Differential follicular dynamics both in the presence of a single dominant follicle (DF) and in codominance during the follicular deviation process were detected in llamas (p < 0.05). The percentage of superficial cells was the most related to the follicular wave phase. However, the percentage of parabasal, intermediate, and superficial cells was not related to the phases of follicular growth, dominance, and regression (p > 0.05). Differential patterns among the different hormone concentration levels regarding the 17β estradiol, anti-Mullerian hormone, progesterone, and testosterone during follicular deviation were observed, with the latter being significantly different along the deviation process (p < 0.05). In conclusion, the use of vaginal cytology assessment would not be sufficient to determine the follicular phases in llamas. Therefore, complementary analyses, such as ultrasonography and endocrine assessment, are strongly recommended to determine follicular dynamics during the follicular deviation.Fil: Perez Guerra, Uri Harold. Universidad Nacional del Altiplano; PerúFil: Quispe, Yesenia M.. Universidad Nacional del Altiplano; PerúFil: Gonzáles, Henry I.. Universidad Nacional del Altiplano; PerúFil: Luque, Natalio. Universidad Nacional del Altiplano; PerúFil: Ruelas, Domingo A.. Universidad Nacional del Altiplano; PerúFil: Carretero, Maria Ignacia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal; ArgentinaFil: Gutiérrez Reinoso, Miguel A.. Universidad Técnica de Cotopaxi; EcuadorFil: Pérez Durand, Manuel G.. Universidad Nacional del Altiplano; PerúFil: García Herreros, Manuel. Instituto Nacional de Investigação Agrária e Veterinária; PortugalMultidisciplinary Digital Publishing Institute2022-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/201473Perez Guerra, Uri Harold; Quispe, Yesenia M.; Gonzáles, Henry I.; Luque, Natalio; Ruelas, Domingo A.; et al.; Ovarian Follicular Dynamics and Its Functional Significance in Relation with Follicle Deviation, Vaginal Cytology, and Hormone Profiles in Llamas (Lama glama); Multidisciplinary Digital Publishing Institute; Animals; 12; 23; 12-2022; 1-162076-2615CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/ani12233299info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:43:04Zoai:ri.conicet.gov.ar:11336/201473instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:43:04.5CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ovarian Follicular Dynamics and Its Functional Significance in Relation with Follicle Deviation, Vaginal Cytology, and Hormone Profiles in Llamas (Lama glama)
title Ovarian Follicular Dynamics and Its Functional Significance in Relation with Follicle Deviation, Vaginal Cytology, and Hormone Profiles in Llamas (Lama glama)
spellingShingle Ovarian Follicular Dynamics and Its Functional Significance in Relation with Follicle Deviation, Vaginal Cytology, and Hormone Profiles in Llamas (Lama glama)
Perez Guerra, Uri Harold
ENDOCRINE PROFILES
FOLLICLE DEVIATION
FOLLICULAR DYNAMICS
LLAMA
VAGINAL CYTOLOGY
title_short Ovarian Follicular Dynamics and Its Functional Significance in Relation with Follicle Deviation, Vaginal Cytology, and Hormone Profiles in Llamas (Lama glama)
title_full Ovarian Follicular Dynamics and Its Functional Significance in Relation with Follicle Deviation, Vaginal Cytology, and Hormone Profiles in Llamas (Lama glama)
title_fullStr Ovarian Follicular Dynamics and Its Functional Significance in Relation with Follicle Deviation, Vaginal Cytology, and Hormone Profiles in Llamas (Lama glama)
title_full_unstemmed Ovarian Follicular Dynamics and Its Functional Significance in Relation with Follicle Deviation, Vaginal Cytology, and Hormone Profiles in Llamas (Lama glama)
title_sort Ovarian Follicular Dynamics and Its Functional Significance in Relation with Follicle Deviation, Vaginal Cytology, and Hormone Profiles in Llamas (Lama glama)
dc.creator.none.fl_str_mv Perez Guerra, Uri Harold
Quispe, Yesenia M.
Gonzáles, Henry I.
Luque, Natalio
Ruelas, Domingo A.
Carretero, Maria Ignacia
Gutiérrez Reinoso, Miguel A.
Pérez Durand, Manuel G.
García Herreros, Manuel
author Perez Guerra, Uri Harold
author_facet Perez Guerra, Uri Harold
Quispe, Yesenia M.
Gonzáles, Henry I.
Luque, Natalio
Ruelas, Domingo A.
Carretero, Maria Ignacia
Gutiérrez Reinoso, Miguel A.
Pérez Durand, Manuel G.
García Herreros, Manuel
author_role author
author2 Quispe, Yesenia M.
Gonzáles, Henry I.
Luque, Natalio
Ruelas, Domingo A.
Carretero, Maria Ignacia
Gutiérrez Reinoso, Miguel A.
Pérez Durand, Manuel G.
García Herreros, Manuel
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ENDOCRINE PROFILES
FOLLICLE DEVIATION
FOLLICULAR DYNAMICS
LLAMA
VAGINAL CYTOLOGY
topic ENDOCRINE PROFILES
FOLLICLE DEVIATION
FOLLICULAR DYNAMICS
LLAMA
VAGINAL CYTOLOGY
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The reproductive physiology in camelid species has its particularities. The present study aimed to characterize the ovarian follicular dynamics and its functional significance in relation to follicular deviation, vaginal cytological characteristics, and sexual hormone profiles in llamas as the first report in South American camelids. Non-pregnant, multiparous llamas (Lama glama; n = 10; age: 48–72 mo.; BCS: 2.5–3.0) were enrolled in the study. The ultrasonographic assessment was carried out transvaginally and follicular ablation was performed (day 0) when follicles were larger than 7 mm. The follicle number and diameter were scored daily throughout the process for a proper evaluation of the deviated follicles and to monitor the presence of new follicle pools (1.5 to 2.5 mm diameter). Vaginal cytological evaluation (parabasal, intermediate, and superficial cells) was performed every other day until day 6. Endocrine profiles (17β estradiol, anti-Mullerian hormone, testosterone, and progesterone) during pre- and post-follicular deviation were determined by using the ELISA assay. Differential follicular dynamics both in the presence of a single dominant follicle (DF) and in codominance during the follicular deviation process were detected in llamas (p < 0.05). The percentage of superficial cells was the most related to the follicular wave phase. However, the percentage of parabasal, intermediate, and superficial cells was not related to the phases of follicular growth, dominance, and regression (p > 0.05). Differential patterns among the different hormone concentration levels regarding the 17β estradiol, anti-Mullerian hormone, progesterone, and testosterone during follicular deviation were observed, with the latter being significantly different along the deviation process (p < 0.05). In conclusion, the use of vaginal cytology assessment would not be sufficient to determine the follicular phases in llamas. Therefore, complementary analyses, such as ultrasonography and endocrine assessment, are strongly recommended to determine follicular dynamics during the follicular deviation.
Fil: Perez Guerra, Uri Harold. Universidad Nacional del Altiplano; Perú
Fil: Quispe, Yesenia M.. Universidad Nacional del Altiplano; Perú
Fil: Gonzáles, Henry I.. Universidad Nacional del Altiplano; Perú
Fil: Luque, Natalio. Universidad Nacional del Altiplano; Perú
Fil: Ruelas, Domingo A.. Universidad Nacional del Altiplano; Perú
Fil: Carretero, Maria Ignacia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal; Argentina
Fil: Gutiérrez Reinoso, Miguel A.. Universidad Técnica de Cotopaxi; Ecuador
Fil: Pérez Durand, Manuel G.. Universidad Nacional del Altiplano; Perú
Fil: García Herreros, Manuel. Instituto Nacional de Investigação Agrária e Veterinária; Portugal
description The reproductive physiology in camelid species has its particularities. The present study aimed to characterize the ovarian follicular dynamics and its functional significance in relation to follicular deviation, vaginal cytological characteristics, and sexual hormone profiles in llamas as the first report in South American camelids. Non-pregnant, multiparous llamas (Lama glama; n = 10; age: 48–72 mo.; BCS: 2.5–3.0) were enrolled in the study. The ultrasonographic assessment was carried out transvaginally and follicular ablation was performed (day 0) when follicles were larger than 7 mm. The follicle number and diameter were scored daily throughout the process for a proper evaluation of the deviated follicles and to monitor the presence of new follicle pools (1.5 to 2.5 mm diameter). Vaginal cytological evaluation (parabasal, intermediate, and superficial cells) was performed every other day until day 6. Endocrine profiles (17β estradiol, anti-Mullerian hormone, testosterone, and progesterone) during pre- and post-follicular deviation were determined by using the ELISA assay. Differential follicular dynamics both in the presence of a single dominant follicle (DF) and in codominance during the follicular deviation process were detected in llamas (p < 0.05). The percentage of superficial cells was the most related to the follicular wave phase. However, the percentage of parabasal, intermediate, and superficial cells was not related to the phases of follicular growth, dominance, and regression (p > 0.05). Differential patterns among the different hormone concentration levels regarding the 17β estradiol, anti-Mullerian hormone, progesterone, and testosterone during follicular deviation were observed, with the latter being significantly different along the deviation process (p < 0.05). In conclusion, the use of vaginal cytology assessment would not be sufficient to determine the follicular phases in llamas. Therefore, complementary analyses, such as ultrasonography and endocrine assessment, are strongly recommended to determine follicular dynamics during the follicular deviation.
publishDate 2022
dc.date.none.fl_str_mv 2022-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/201473
Perez Guerra, Uri Harold; Quispe, Yesenia M.; Gonzáles, Henry I.; Luque, Natalio; Ruelas, Domingo A.; et al.; Ovarian Follicular Dynamics and Its Functional Significance in Relation with Follicle Deviation, Vaginal Cytology, and Hormone Profiles in Llamas (Lama glama); Multidisciplinary Digital Publishing Institute; Animals; 12; 23; 12-2022; 1-16
2076-2615
CONICET Digital
CONICET
url http://hdl.handle.net/11336/201473
identifier_str_mv Perez Guerra, Uri Harold; Quispe, Yesenia M.; Gonzáles, Henry I.; Luque, Natalio; Ruelas, Domingo A.; et al.; Ovarian Follicular Dynamics and Its Functional Significance in Relation with Follicle Deviation, Vaginal Cytology, and Hormone Profiles in Llamas (Lama glama); Multidisciplinary Digital Publishing Institute; Animals; 12; 23; 12-2022; 1-16
2076-2615
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/ani12233299
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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