Metabolic profiling of preovulatory follicular fluid in jennies

Autores
Catalán, Jaime; Martínez Rodero, Iris; Yánez Ortiz, Iván; Mateo Otero, Yentel; Flores Bragulat, Ana Paula; Nolis, Pau; Carluccio, Augusto; Yeste, Marc; Miró, Jordi
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Follicular fluid is formed from the transudation of theca and granulosa cells in the growing follicular antrum. Its main function is to provide an optimal intrafollicular microenvironment to modulate oocyte maturation. The aim of this study was to determine the metabolomic profile of preovulatory follicular fluid (PFF) in jennies. For this purpose, PFF was collected from 10 follicles of five jennies in heat. Then, PFF samples were analysed by nuclear magnetic resonance (NMR) and heteronuclear single quantum correlation (2D 1H/13C HSQC). Our study revealed the presence of at least 27 metabolites in the PFF of jennies (including common amino acids, carboxylic acids, amino acid derivatives, alcohols, saccharides, fatty acids, and lactams): 3-hydroxybutyrate, acetate, alanine, betaine, citrate, creatine, creatine phosphate, creatinine, ethanol, formate, glucose, glutamine, glycerol, glycine, hippurate, isoleucine, lactate, leucine, lysine, methanol, phenylalanine, proline, pyruvate, threonine, tyrosine, valine, and τ-methylhistidine. The metabolites found here have an important role in the oocyte development and maturation, since the PFF surrounds the follicle and provides it with the needed nutrients. Our results indicate a unique metabolic profile of the jennies PFF, as it differs from those previously observed in the PFF of the mare, a phylogenetically close species that is taken as a reference for establishing reproductive biotechnology techniques in donkeys. The metabolites found here also differ from those described in the TCM-199 medium enriched with fetal bovine serum (FBS), which is the most used medium for in vitro oocyte maturation in equids. These differences would suggest that the established conditions for in vitro maturation used so far may not be suitable for donkeys. By providing the metabolic composition of jenny PFF, this study could help understand the physiology of oocyte maturation as a first step to establish in vitro reproductive techniques in this species.
Fil: Catalán, Jaime. Universidad de Girona; España. Universitat Autònoma de Barcelona; España
Fil: Martínez Rodero, Iris. Universitat Autònoma de Barcelona; España
Fil: Yánez Ortiz, Iván. Universitat Autònoma de Barcelona; España. Universidad de Girona; España
Fil: Mateo Otero, Yentel. Universidad de Girona; España
Fil: Flores Bragulat, Ana Paula. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Nolis, Pau. Universitat Autònoma de Barcelona; España
Fil: Carluccio, Augusto. University of Teramo; Italia
Fil: Yeste, Marc. Universidad de Girona; España
Fil: Miró, Jordi. Universitat Autònoma de Barcelona; España
Materia
DONKEY
EQUIDS
FOLLICULAR FLUID
METABOLITES
METABOLOMIC
OVARY
PREOVULATORY FOLLICLE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/217752
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/217752
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Metabolic profiling of preovulatory follicular fluid in jenniesCatalán, JaimeMartínez Rodero, IrisYánez Ortiz, IvánMateo Otero, YentelFlores Bragulat, Ana PaulaNolis, PauCarluccio, AugustoYeste, MarcMiró, JordiDONKEYEQUIDSFOLLICULAR FLUIDMETABOLITESMETABOLOMICOVARYPREOVULATORY FOLLICLEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Follicular fluid is formed from the transudation of theca and granulosa cells in the growing follicular antrum. Its main function is to provide an optimal intrafollicular microenvironment to modulate oocyte maturation. The aim of this study was to determine the metabolomic profile of preovulatory follicular fluid (PFF) in jennies. For this purpose, PFF was collected from 10 follicles of five jennies in heat. Then, PFF samples were analysed by nuclear magnetic resonance (NMR) and heteronuclear single quantum correlation (2D 1H/13C HSQC). Our study revealed the presence of at least 27 metabolites in the PFF of jennies (including common amino acids, carboxylic acids, amino acid derivatives, alcohols, saccharides, fatty acids, and lactams): 3-hydroxybutyrate, acetate, alanine, betaine, citrate, creatine, creatine phosphate, creatinine, ethanol, formate, glucose, glutamine, glycerol, glycine, hippurate, isoleucine, lactate, leucine, lysine, methanol, phenylalanine, proline, pyruvate, threonine, tyrosine, valine, and τ-methylhistidine. The metabolites found here have an important role in the oocyte development and maturation, since the PFF surrounds the follicle and provides it with the needed nutrients. Our results indicate a unique metabolic profile of the jennies PFF, as it differs from those previously observed in the PFF of the mare, a phylogenetically close species that is taken as a reference for establishing reproductive biotechnology techniques in donkeys. The metabolites found here also differ from those described in the TCM-199 medium enriched with fetal bovine serum (FBS), which is the most used medium for in vitro oocyte maturation in equids. These differences would suggest that the established conditions for in vitro maturation used so far may not be suitable for donkeys. By providing the metabolic composition of jenny PFF, this study could help understand the physiology of oocyte maturation as a first step to establish in vitro reproductive techniques in this species.Fil: Catalán, Jaime. Universidad de Girona; España. Universitat Autònoma de Barcelona; EspañaFil: Martínez Rodero, Iris. Universitat Autònoma de Barcelona; EspañaFil: Yánez Ortiz, Iván. Universitat Autònoma de Barcelona; España. Universidad de Girona; EspañaFil: Mateo Otero, Yentel. Universidad de Girona; EspañaFil: Flores Bragulat, Ana Paula. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Nolis, Pau. Universitat Autònoma de Barcelona; EspañaFil: Carluccio, Augusto. University of Teramo; ItaliaFil: Yeste, Marc. Universidad de Girona; EspañaFil: Miró, Jordi. Universitat Autònoma de Barcelona; EspañaElsevier2022-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/217752Catalán, Jaime; Martínez Rodero, Iris; Yánez Ortiz, Iván; Mateo Otero, Yentel; Flores Bragulat, Ana Paula; et al.; Metabolic profiling of preovulatory follicular fluid in jennies; Elsevier; Research in Veterinary Science; 153; 12-2022; 127-1360034-5288CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.rvsc.2022.10.026info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:14Zoai:ri.conicet.gov.ar:11336/217752instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:14.906CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Metabolic profiling of preovulatory follicular fluid in jennies
title Metabolic profiling of preovulatory follicular fluid in jennies
spellingShingle Metabolic profiling of preovulatory follicular fluid in jennies
Catalán, Jaime
DONKEY
EQUIDS
FOLLICULAR FLUID
METABOLITES
METABOLOMIC
OVARY
PREOVULATORY FOLLICLE
title_short Metabolic profiling of preovulatory follicular fluid in jennies
title_full Metabolic profiling of preovulatory follicular fluid in jennies
title_fullStr Metabolic profiling of preovulatory follicular fluid in jennies
title_full_unstemmed Metabolic profiling of preovulatory follicular fluid in jennies
title_sort Metabolic profiling of preovulatory follicular fluid in jennies
dc.creator.none.fl_str_mv Catalán, Jaime
Martínez Rodero, Iris
Yánez Ortiz, Iván
Mateo Otero, Yentel
Flores Bragulat, Ana Paula
Nolis, Pau
Carluccio, Augusto
Yeste, Marc
Miró, Jordi
author Catalán, Jaime
author_facet Catalán, Jaime
Martínez Rodero, Iris
Yánez Ortiz, Iván
Mateo Otero, Yentel
Flores Bragulat, Ana Paula
Nolis, Pau
Carluccio, Augusto
Yeste, Marc
Miró, Jordi
author_role author
author2 Martínez Rodero, Iris
Yánez Ortiz, Iván
Mateo Otero, Yentel
Flores Bragulat, Ana Paula
Nolis, Pau
Carluccio, Augusto
Yeste, Marc
Miró, Jordi
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DONKEY
EQUIDS
FOLLICULAR FLUID
METABOLITES
METABOLOMIC
OVARY
PREOVULATORY FOLLICLE
topic DONKEY
EQUIDS
FOLLICULAR FLUID
METABOLITES
METABOLOMIC
OVARY
PREOVULATORY FOLLICLE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Follicular fluid is formed from the transudation of theca and granulosa cells in the growing follicular antrum. Its main function is to provide an optimal intrafollicular microenvironment to modulate oocyte maturation. The aim of this study was to determine the metabolomic profile of preovulatory follicular fluid (PFF) in jennies. For this purpose, PFF was collected from 10 follicles of five jennies in heat. Then, PFF samples were analysed by nuclear magnetic resonance (NMR) and heteronuclear single quantum correlation (2D 1H/13C HSQC). Our study revealed the presence of at least 27 metabolites in the PFF of jennies (including common amino acids, carboxylic acids, amino acid derivatives, alcohols, saccharides, fatty acids, and lactams): 3-hydroxybutyrate, acetate, alanine, betaine, citrate, creatine, creatine phosphate, creatinine, ethanol, formate, glucose, glutamine, glycerol, glycine, hippurate, isoleucine, lactate, leucine, lysine, methanol, phenylalanine, proline, pyruvate, threonine, tyrosine, valine, and τ-methylhistidine. The metabolites found here have an important role in the oocyte development and maturation, since the PFF surrounds the follicle and provides it with the needed nutrients. Our results indicate a unique metabolic profile of the jennies PFF, as it differs from those previously observed in the PFF of the mare, a phylogenetically close species that is taken as a reference for establishing reproductive biotechnology techniques in donkeys. The metabolites found here also differ from those described in the TCM-199 medium enriched with fetal bovine serum (FBS), which is the most used medium for in vitro oocyte maturation in equids. These differences would suggest that the established conditions for in vitro maturation used so far may not be suitable for donkeys. By providing the metabolic composition of jenny PFF, this study could help understand the physiology of oocyte maturation as a first step to establish in vitro reproductive techniques in this species.
Fil: Catalán, Jaime. Universidad de Girona; España. Universitat Autònoma de Barcelona; España
Fil: Martínez Rodero, Iris. Universitat Autònoma de Barcelona; España
Fil: Yánez Ortiz, Iván. Universitat Autònoma de Barcelona; España. Universidad de Girona; España
Fil: Mateo Otero, Yentel. Universidad de Girona; España
Fil: Flores Bragulat, Ana Paula. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Nolis, Pau. Universitat Autònoma de Barcelona; España
Fil: Carluccio, Augusto. University of Teramo; Italia
Fil: Yeste, Marc. Universidad de Girona; España
Fil: Miró, Jordi. Universitat Autònoma de Barcelona; España
description Follicular fluid is formed from the transudation of theca and granulosa cells in the growing follicular antrum. Its main function is to provide an optimal intrafollicular microenvironment to modulate oocyte maturation. The aim of this study was to determine the metabolomic profile of preovulatory follicular fluid (PFF) in jennies. For this purpose, PFF was collected from 10 follicles of five jennies in heat. Then, PFF samples were analysed by nuclear magnetic resonance (NMR) and heteronuclear single quantum correlation (2D 1H/13C HSQC). Our study revealed the presence of at least 27 metabolites in the PFF of jennies (including common amino acids, carboxylic acids, amino acid derivatives, alcohols, saccharides, fatty acids, and lactams): 3-hydroxybutyrate, acetate, alanine, betaine, citrate, creatine, creatine phosphate, creatinine, ethanol, formate, glucose, glutamine, glycerol, glycine, hippurate, isoleucine, lactate, leucine, lysine, methanol, phenylalanine, proline, pyruvate, threonine, tyrosine, valine, and τ-methylhistidine. The metabolites found here have an important role in the oocyte development and maturation, since the PFF surrounds the follicle and provides it with the needed nutrients. Our results indicate a unique metabolic profile of the jennies PFF, as it differs from those previously observed in the PFF of the mare, a phylogenetically close species that is taken as a reference for establishing reproductive biotechnology techniques in donkeys. The metabolites found here also differ from those described in the TCM-199 medium enriched with fetal bovine serum (FBS), which is the most used medium for in vitro oocyte maturation in equids. These differences would suggest that the established conditions for in vitro maturation used so far may not be suitable for donkeys. By providing the metabolic composition of jenny PFF, this study could help understand the physiology of oocyte maturation as a first step to establish in vitro reproductive techniques in this species.
publishDate 2022
dc.date.none.fl_str_mv 2022-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/217752
Catalán, Jaime; Martínez Rodero, Iris; Yánez Ortiz, Iván; Mateo Otero, Yentel; Flores Bragulat, Ana Paula; et al.; Metabolic profiling of preovulatory follicular fluid in jennies; Elsevier; Research in Veterinary Science; 153; 12-2022; 127-136
0034-5288
CONICET Digital
CONICET
url http://hdl.handle.net/11336/217752
identifier_str_mv Catalán, Jaime; Martínez Rodero, Iris; Yánez Ortiz, Iván; Mateo Otero, Yentel; Flores Bragulat, Ana Paula; et al.; Metabolic profiling of preovulatory follicular fluid in jennies; Elsevier; Research in Veterinary Science; 153; 12-2022; 127-136
0034-5288
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.rvsc.2022.10.026
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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