Reconstitution reveals how myosin-VI self- organises to generate a dynamic mechanism of membrane sculpting

Autores
Rogez, Benoit; Würthner, Laeschkir; Petrova, Anastasiia B.; Zierhut, Felix B.; Saczko Brack, Dario; Huergo, María Ana Cristina; Batters, Christopher; Frey, Erwin; Veigel, Claudia
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
One enigma in biology is the generation, sensing and maintenance of membrane curvature. Curvature-mediating proteins have been shown to induce specific membrane shapes by direct insertion and nanoscopic scaffolding, while the cytoskeletal motors exert forces indirectly through microtubule and actin networks. It remains unclear, whether the manifold direct motorprotein–lipid interactions themselves constitute another fundamental route to remodel the membrane shape. Here we show, combining super-resolution-fluorescence microscopy and membrane-reshaping nanoparticles, that curvature-dependent lipid interactions of myosin-VI on its own, remarkably remodel the membrane geometry into dynamic spatial patterns on the nano- to micrometer scale. We propose a quantitative theoretical model that explains this dynamic membrane sculpting mechanism. The emerging route of motorprotein–lipid interactions reshaping membrane morphology by a mechanism of feedback and instability opens up hitherto unexplored avenues of membrane remodelling and links cytoskeletal motors to early events in the sequence of membrane sculpting in eukaryotic cell biology.
Fil: Rogez, Benoit. Ludwig Maximilians Universitat; Alemania
Fil: Würthner, Laeschkir. Ludwig Maximilians Universitat; Alemania
Fil: Petrova, Anastasiia B.. Ludwig Maximilians Universitat; Alemania
Fil: Zierhut, Felix B.. Ludwig Maximilians Universitat; Alemania
Fil: Saczko Brack, Dario. Ludwig Maximilians Universitat; Alemania
Fil: Huergo, María Ana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Batters, Christopher. Ludwig Maximilians Universitat; Alemania
Fil: Frey, Erwin. Ludwig Maximilians Universitat; Alemania
Fil: Veigel, Claudia. Ludwig Maximilians Universitat; Alemania
Materia
MIOSIN VI
MEMBRANE SCULPTING
SUPER RESOLUTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/118660
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Reconstitution reveals how myosin-VI self- organises to generate a dynamic mechanism of membrane sculptingRogez, BenoitWürthner, LaeschkirPetrova, Anastasiia B.Zierhut, Felix B.Saczko Brack, DarioHuergo, María Ana CristinaBatters, ChristopherFrey, ErwinVeigel, ClaudiaMIOSIN VIMEMBRANE SCULPTINGSUPER RESOLUTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1One enigma in biology is the generation, sensing and maintenance of membrane curvature. Curvature-mediating proteins have been shown to induce specific membrane shapes by direct insertion and nanoscopic scaffolding, while the cytoskeletal motors exert forces indirectly through microtubule and actin networks. It remains unclear, whether the manifold direct motorprotein–lipid interactions themselves constitute another fundamental route to remodel the membrane shape. Here we show, combining super-resolution-fluorescence microscopy and membrane-reshaping nanoparticles, that curvature-dependent lipid interactions of myosin-VI on its own, remarkably remodel the membrane geometry into dynamic spatial patterns on the nano- to micrometer scale. We propose a quantitative theoretical model that explains this dynamic membrane sculpting mechanism. The emerging route of motorprotein–lipid interactions reshaping membrane morphology by a mechanism of feedback and instability opens up hitherto unexplored avenues of membrane remodelling and links cytoskeletal motors to early events in the sequence of membrane sculpting in eukaryotic cell biology.Fil: Rogez, Benoit. Ludwig Maximilians Universitat; AlemaniaFil: Würthner, Laeschkir. Ludwig Maximilians Universitat; AlemaniaFil: Petrova, Anastasiia B.. Ludwig Maximilians Universitat; AlemaniaFil: Zierhut, Felix B.. Ludwig Maximilians Universitat; AlemaniaFil: Saczko Brack, Dario. Ludwig Maximilians Universitat; AlemaniaFil: Huergo, María Ana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Batters, Christopher. Ludwig Maximilians Universitat; AlemaniaFil: Frey, Erwin. Ludwig Maximilians Universitat; AlemaniaFil: Veigel, Claudia. Ludwig Maximilians Universitat; AlemaniaSpringer2019-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/118660Rogez, Benoit ; Würthner, Laeschkir ; Petrova, Anastasiia B. ; Zierhut, Felix B. ; Saczko Brack, Dario ; et al.; Reconstitution reveals how myosin-VI self- organises to generate a dynamic mechanism of membrane sculpting; Springer; Nature Communications; 10; 3305; 7-2019; 1-112041-1723CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/www.nature.com/naturecommunicationsinfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-019-11268-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-04-15T10:19:54Zoai:ri.conicet.gov.ar:11336/118660instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-04-15 10:19:55.304CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Reconstitution reveals how myosin-VI self- organises to generate a dynamic mechanism of membrane sculpting
title Reconstitution reveals how myosin-VI self- organises to generate a dynamic mechanism of membrane sculpting
spellingShingle Reconstitution reveals how myosin-VI self- organises to generate a dynamic mechanism of membrane sculpting
Rogez, Benoit
MIOSIN VI
MEMBRANE SCULPTING
SUPER RESOLUTION
title_short Reconstitution reveals how myosin-VI self- organises to generate a dynamic mechanism of membrane sculpting
title_full Reconstitution reveals how myosin-VI self- organises to generate a dynamic mechanism of membrane sculpting
title_fullStr Reconstitution reveals how myosin-VI self- organises to generate a dynamic mechanism of membrane sculpting
title_full_unstemmed Reconstitution reveals how myosin-VI self- organises to generate a dynamic mechanism of membrane sculpting
title_sort Reconstitution reveals how myosin-VI self- organises to generate a dynamic mechanism of membrane sculpting
dc.creator.none.fl_str_mv Rogez, Benoit
Würthner, Laeschkir
Petrova, Anastasiia B.
Zierhut, Felix B.
Saczko Brack, Dario
Huergo, María Ana Cristina
Batters, Christopher
Frey, Erwin
Veigel, Claudia
author Rogez, Benoit
author_facet Rogez, Benoit
Würthner, Laeschkir
Petrova, Anastasiia B.
Zierhut, Felix B.
Saczko Brack, Dario
Huergo, María Ana Cristina
Batters, Christopher
Frey, Erwin
Veigel, Claudia
author_role author
author2 Würthner, Laeschkir
Petrova, Anastasiia B.
Zierhut, Felix B.
Saczko Brack, Dario
Huergo, María Ana Cristina
Batters, Christopher
Frey, Erwin
Veigel, Claudia
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv MIOSIN VI
MEMBRANE SCULPTING
SUPER RESOLUTION
topic MIOSIN VI
MEMBRANE SCULPTING
SUPER RESOLUTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv One enigma in biology is the generation, sensing and maintenance of membrane curvature. Curvature-mediating proteins have been shown to induce specific membrane shapes by direct insertion and nanoscopic scaffolding, while the cytoskeletal motors exert forces indirectly through microtubule and actin networks. It remains unclear, whether the manifold direct motorprotein–lipid interactions themselves constitute another fundamental route to remodel the membrane shape. Here we show, combining super-resolution-fluorescence microscopy and membrane-reshaping nanoparticles, that curvature-dependent lipid interactions of myosin-VI on its own, remarkably remodel the membrane geometry into dynamic spatial patterns on the nano- to micrometer scale. We propose a quantitative theoretical model that explains this dynamic membrane sculpting mechanism. The emerging route of motorprotein–lipid interactions reshaping membrane morphology by a mechanism of feedback and instability opens up hitherto unexplored avenues of membrane remodelling and links cytoskeletal motors to early events in the sequence of membrane sculpting in eukaryotic cell biology.
Fil: Rogez, Benoit. Ludwig Maximilians Universitat; Alemania
Fil: Würthner, Laeschkir. Ludwig Maximilians Universitat; Alemania
Fil: Petrova, Anastasiia B.. Ludwig Maximilians Universitat; Alemania
Fil: Zierhut, Felix B.. Ludwig Maximilians Universitat; Alemania
Fil: Saczko Brack, Dario. Ludwig Maximilians Universitat; Alemania
Fil: Huergo, María Ana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Batters, Christopher. Ludwig Maximilians Universitat; Alemania
Fil: Frey, Erwin. Ludwig Maximilians Universitat; Alemania
Fil: Veigel, Claudia. Ludwig Maximilians Universitat; Alemania
description One enigma in biology is the generation, sensing and maintenance of membrane curvature. Curvature-mediating proteins have been shown to induce specific membrane shapes by direct insertion and nanoscopic scaffolding, while the cytoskeletal motors exert forces indirectly through microtubule and actin networks. It remains unclear, whether the manifold direct motorprotein–lipid interactions themselves constitute another fundamental route to remodel the membrane shape. Here we show, combining super-resolution-fluorescence microscopy and membrane-reshaping nanoparticles, that curvature-dependent lipid interactions of myosin-VI on its own, remarkably remodel the membrane geometry into dynamic spatial patterns on the nano- to micrometer scale. We propose a quantitative theoretical model that explains this dynamic membrane sculpting mechanism. The emerging route of motorprotein–lipid interactions reshaping membrane morphology by a mechanism of feedback and instability opens up hitherto unexplored avenues of membrane remodelling and links cytoskeletal motors to early events in the sequence of membrane sculpting in eukaryotic cell biology.
publishDate 2019
dc.date.none.fl_str_mv 2019-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/118660
Rogez, Benoit ; Würthner, Laeschkir ; Petrova, Anastasiia B. ; Zierhut, Felix B. ; Saczko Brack, Dario ; et al.; Reconstitution reveals how myosin-VI self- organises to generate a dynamic mechanism of membrane sculpting; Springer; Nature Communications; 10; 3305; 7-2019; 1-11
2041-1723
CONICET Digital
CONICET
url http://hdl.handle.net/11336/118660
identifier_str_mv Rogez, Benoit ; Würthner, Laeschkir ; Petrova, Anastasiia B. ; Zierhut, Felix B. ; Saczko Brack, Dario ; et al.; Reconstitution reveals how myosin-VI self- organises to generate a dynamic mechanism of membrane sculpting; Springer; Nature Communications; 10; 3305; 7-2019; 1-11
2041-1723
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/www.nature.com/naturecommunications
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-019-11268-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1862632389364154368
score 13.203462

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