High LDL levels are associated with increased lipoprotein-associated phospholipase A2 activity on nitric oxide synthesis and reactive oxygen species formation in human endothelial...

Autores
Searle, Andrea; Gomez Rosso, Leonardo Adrián; Meroño, Tomás; Salomon, Carlos; Durán Sandoval, Daniel; Giunta, Gustavo Ariel; Grant, Carlos; Calvo, Carlos; Lamperti, Liliana; Brites, Fernando Daniel; Aguayo, Claudio
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: To evaluate in vitro the effects of serum and LDL fractions isolated from hypercholesterolemic patients on nitric oxide (NO) synthesis and reactive oxygen species (ROS) production by human umbilical vein endothelial cells (HUVECs). Design and methods: Serum and LDL isolated from subjects with high (n=18) and normal (n=21) LDLcholesterol levels were analyzed on NO synthesis and ROS production in vitro models of HUVECs. LDL was furthers characterized in their chemical composition and activities of lipoprotein-associated phospholipase A2 (Lp-PLA2), cholesteryl ester transfer protein (CETP) and paraoxonase. Results: NO bioavailability was significantly lower and ROS production higher in HUVECs incubated with
serum samples from patients with high LDL-cholesterol levels in comparison to control subjects. Moreover, hypercholesterolemic patients presented higher CETP and Lp-PLA2 activities than control subjects. LDL fractions isolated from patients and controls were not different in their chemical composition, Lp-PLA2 activity, and their capacity to reduce NO synthesis and increase ROS production. Conclusion: Alterations of serum from hypercholesterolemic patients could be due to the increment in LDL concentration, main Lp-PLA2 carrier, and not to LDL composition or intrinsic Lp-PLA2 activity.
Fil: Searle, Andrea. Universidad de Concepción; Chile
Fil: Gomez Rosso, Leonardo Adrián. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Meroño, Tomás. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Salomon, Carlos. Universidad de Concepción; Chile
Fil: Durán Sandoval, Daniel. Universidad de Concepción; Chile
Fil: Giunta, Gustavo Ariel. Fundacion Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Grant, Carlos. Universidad de Concepción; Chile
Fil: Calvo, Carlos. Universidad de Concepción; Chile
Fil: Lamperti, Liliana. Universidad de Concepción; Chile
Fil: Brites, Fernando Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Aguayo, Claudio. Universidad de Concepción; Chile
Materia
Reactive Oxygen Species
Lipoprotein Associated Phospholipase A2
Ldl
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/12888
Acceder
id CONICETDig_fa622153fcdc565a44bf3fb14d2547a8
oai_identifier_str oai:ri.conicet.gov.ar:11336/12888
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling High LDL levels are associated with increased lipoprotein-associated phospholipase A2 activity on nitric oxide synthesis and reactive oxygen species formation in human endothelial cellsSearle, AndreaGomez Rosso, Leonardo AdriánMeroño, TomásSalomon, CarlosDurán Sandoval, DanielGiunta, Gustavo ArielGrant, CarlosCalvo, CarlosLamperti, LilianaBrites, Fernando DanielAguayo, ClaudioReactive Oxygen SpeciesLipoprotein Associated Phospholipase A2Ldlhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Objective: To evaluate in vitro the effects of serum and LDL fractions isolated from hypercholesterolemic patients on nitric oxide (NO) synthesis and reactive oxygen species (ROS) production by human umbilical vein endothelial cells (HUVECs). Design and methods: Serum and LDL isolated from subjects with high (n=18) and normal (n=21) LDLcholesterol levels were analyzed on NO synthesis and ROS production in vitro models of HUVECs. LDL was furthers characterized in their chemical composition and activities of lipoprotein-associated phospholipase A2 (Lp-PLA2), cholesteryl ester transfer protein (CETP) and paraoxonase. Results: NO bioavailability was significantly lower and ROS production higher in HUVECs incubated with<br />serum samples from patients with high LDL-cholesterol levels in comparison to control subjects. Moreover, hypercholesterolemic patients presented higher CETP and Lp-PLA2 activities than control subjects. LDL fractions isolated from patients and controls were not different in their chemical composition, Lp-PLA2 activity, and their capacity to reduce NO synthesis and increase ROS production. Conclusion: Alterations of serum from hypercholesterolemic patients could be due to the increment in LDL concentration, main Lp-PLA2 carrier, and not to LDL composition or intrinsic Lp-PLA2 activity.Fil: Searle, Andrea. Universidad de Concepción; ChileFil: Gomez Rosso, Leonardo Adrián. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Meroño, Tomás. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Salomon, Carlos. Universidad de Concepción; ChileFil: Durán Sandoval, Daniel. Universidad de Concepción; ChileFil: Giunta, Gustavo Ariel. Fundacion Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Grant, Carlos. Universidad de Concepción; ChileFil: Calvo, Carlos. Universidad de Concepción; ChileFil: Lamperti, Liliana. Universidad de Concepción; ChileFil: Brites, Fernando Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Aguayo, Claudio. Universidad de Concepción; ChileElsevier2011-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/12888Searle, Andrea; Gomez Rosso, Leonardo Adrián; Meroño, Tomás; Salomon, Carlos; Durán Sandoval, Daniel; et al.; High LDL levels are associated with increased lipoprotein-associated phospholipase A2 activity on nitric oxide synthesis and reactive oxygen species formation in human endothelial cells; Elsevier; Clinical Biochemistry; 44; 2-3; 2-2011; 171-1770009-9120enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0009912010004637info:eu-repo/semantics/altIdentifier/doi/10.1016/j.clinbiochem.2010.10.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:02Zoai:ri.conicet.gov.ar:11336/12888instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:02.839CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv High LDL levels are associated with increased lipoprotein-associated phospholipase A2 activity on nitric oxide synthesis and reactive oxygen species formation in human endothelial cells
title High LDL levels are associated with increased lipoprotein-associated phospholipase A2 activity on nitric oxide synthesis and reactive oxygen species formation in human endothelial cells
spellingShingle High LDL levels are associated with increased lipoprotein-associated phospholipase A2 activity on nitric oxide synthesis and reactive oxygen species formation in human endothelial cells
Searle, Andrea
Reactive Oxygen Species
Lipoprotein Associated Phospholipase A2
Ldl
title_short High LDL levels are associated with increased lipoprotein-associated phospholipase A2 activity on nitric oxide synthesis and reactive oxygen species formation in human endothelial cells
title_full High LDL levels are associated with increased lipoprotein-associated phospholipase A2 activity on nitric oxide synthesis and reactive oxygen species formation in human endothelial cells
title_fullStr High LDL levels are associated with increased lipoprotein-associated phospholipase A2 activity on nitric oxide synthesis and reactive oxygen species formation in human endothelial cells
title_full_unstemmed High LDL levels are associated with increased lipoprotein-associated phospholipase A2 activity on nitric oxide synthesis and reactive oxygen species formation in human endothelial cells
title_sort High LDL levels are associated with increased lipoprotein-associated phospholipase A2 activity on nitric oxide synthesis and reactive oxygen species formation in human endothelial cells
dc.creator.none.fl_str_mv Searle, Andrea
Gomez Rosso, Leonardo Adrián
Meroño, Tomás
Salomon, Carlos
Durán Sandoval, Daniel
Giunta, Gustavo Ariel
Grant, Carlos
Calvo, Carlos
Lamperti, Liliana
Brites, Fernando Daniel
Aguayo, Claudio
author Searle, Andrea
author_facet Searle, Andrea
Gomez Rosso, Leonardo Adrián
Meroño, Tomás
Salomon, Carlos
Durán Sandoval, Daniel
Giunta, Gustavo Ariel
Grant, Carlos
Calvo, Carlos
Lamperti, Liliana
Brites, Fernando Daniel
Aguayo, Claudio
author_role author
author2 Gomez Rosso, Leonardo Adrián
Meroño, Tomás
Salomon, Carlos
Durán Sandoval, Daniel
Giunta, Gustavo Ariel
Grant, Carlos
Calvo, Carlos
Lamperti, Liliana
Brites, Fernando Daniel
Aguayo, Claudio
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Reactive Oxygen Species
Lipoprotein Associated Phospholipase A2
Ldl
topic Reactive Oxygen Species
Lipoprotein Associated Phospholipase A2
Ldl
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Objective: To evaluate in vitro the effects of serum and LDL fractions isolated from hypercholesterolemic patients on nitric oxide (NO) synthesis and reactive oxygen species (ROS) production by human umbilical vein endothelial cells (HUVECs). Design and methods: Serum and LDL isolated from subjects with high (n=18) and normal (n=21) LDLcholesterol levels were analyzed on NO synthesis and ROS production in vitro models of HUVECs. LDL was furthers characterized in their chemical composition and activities of lipoprotein-associated phospholipase A2 (Lp-PLA2), cholesteryl ester transfer protein (CETP) and paraoxonase. Results: NO bioavailability was significantly lower and ROS production higher in HUVECs incubated with<br />serum samples from patients with high LDL-cholesterol levels in comparison to control subjects. Moreover, hypercholesterolemic patients presented higher CETP and Lp-PLA2 activities than control subjects. LDL fractions isolated from patients and controls were not different in their chemical composition, Lp-PLA2 activity, and their capacity to reduce NO synthesis and increase ROS production. Conclusion: Alterations of serum from hypercholesterolemic patients could be due to the increment in LDL concentration, main Lp-PLA2 carrier, and not to LDL composition or intrinsic Lp-PLA2 activity.
Fil: Searle, Andrea. Universidad de Concepción; Chile
Fil: Gomez Rosso, Leonardo Adrián. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Meroño, Tomás. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Salomon, Carlos. Universidad de Concepción; Chile
Fil: Durán Sandoval, Daniel. Universidad de Concepción; Chile
Fil: Giunta, Gustavo Ariel. Fundacion Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Grant, Carlos. Universidad de Concepción; Chile
Fil: Calvo, Carlos. Universidad de Concepción; Chile
Fil: Lamperti, Liliana. Universidad de Concepción; Chile
Fil: Brites, Fernando Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Aguayo, Claudio. Universidad de Concepción; Chile
description Objective: To evaluate in vitro the effects of serum and LDL fractions isolated from hypercholesterolemic patients on nitric oxide (NO) synthesis and reactive oxygen species (ROS) production by human umbilical vein endothelial cells (HUVECs). Design and methods: Serum and LDL isolated from subjects with high (n=18) and normal (n=21) LDLcholesterol levels were analyzed on NO synthesis and ROS production in vitro models of HUVECs. LDL was furthers characterized in their chemical composition and activities of lipoprotein-associated phospholipase A2 (Lp-PLA2), cholesteryl ester transfer protein (CETP) and paraoxonase. Results: NO bioavailability was significantly lower and ROS production higher in HUVECs incubated with<br />serum samples from patients with high LDL-cholesterol levels in comparison to control subjects. Moreover, hypercholesterolemic patients presented higher CETP and Lp-PLA2 activities than control subjects. LDL fractions isolated from patients and controls were not different in their chemical composition, Lp-PLA2 activity, and their capacity to reduce NO synthesis and increase ROS production. Conclusion: Alterations of serum from hypercholesterolemic patients could be due to the increment in LDL concentration, main Lp-PLA2 carrier, and not to LDL composition or intrinsic Lp-PLA2 activity.
publishDate 2011
dc.date.none.fl_str_mv 2011-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/12888
Searle, Andrea; Gomez Rosso, Leonardo Adrián; Meroño, Tomás; Salomon, Carlos; Durán Sandoval, Daniel; et al.; High LDL levels are associated with increased lipoprotein-associated phospholipase A2 activity on nitric oxide synthesis and reactive oxygen species formation in human endothelial cells; Elsevier; Clinical Biochemistry; 44; 2-3; 2-2011; 171-177
0009-9120
url http://hdl.handle.net/11336/12888
identifier_str_mv Searle, Andrea; Gomez Rosso, Leonardo Adrián; Meroño, Tomás; Salomon, Carlos; Durán Sandoval, Daniel; et al.; High LDL levels are associated with increased lipoprotein-associated phospholipase A2 activity on nitric oxide synthesis and reactive oxygen species formation in human endothelial cells; Elsevier; Clinical Biochemistry; 44; 2-3; 2-2011; 171-177
0009-9120
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0009912010004637
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.clinbiochem.2010.10.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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