Mode of action of lactocin 705, a two-component bacteriocin from Lactobacillus casei CRL705

Autores
Castellano, Patricia Haydee; Raya, Raul Ricardo; Vignolo, Graciela Margarita
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lactocin 705 is a bacteriocin whose activity depends on the complementary action of two peptides (705α and 705β) of 33-amino-acid residues each and is produced by Lactobacillus casei CRL705. Biologically active, synthetic lactocin 705 was used to study the mode of action on sensitive cells of Lactobacillus plantarum CRL691. The addition of 90 nmol l-1 of lactocin 705 to cells of L. plantarum dissipated both, the membrane potential (ΔΨ) and the pH gradient (ΔpH). Energized membrane, obtained after the addition of glucose, were more susceptible to lactocin 705 action leading to the immediate release of intracellular K+ and inorganic phosphate. When the role of various ions on sensitive cells were analyzed, only Ca2+ ion exhibited a protective effect against lactocin 705. These data suggest that the presence of a proton motive force (PMF) promotes the interaction of the bacteriocin with the cytoplasmic membrane of energized cells, leading to pore formation which allows for the efflux of ions, thereby ensuring efficient killing of target bacteria.
Fil: Castellano, Patricia Haydee. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Raya, Raul Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Vignolo, Graciela Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
Bacteriocin
Lactobacillus
Mode of Action
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/58656
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mode of action of lactocin 705, a two-component bacteriocin from Lactobacillus casei CRL705Castellano, Patricia HaydeeRaya, Raul RicardoVignolo, Graciela MargaritaBacteriocinLactobacillusMode of Actionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Lactocin 705 is a bacteriocin whose activity depends on the complementary action of two peptides (705α and 705β) of 33-amino-acid residues each and is produced by Lactobacillus casei CRL705. Biologically active, synthetic lactocin 705 was used to study the mode of action on sensitive cells of Lactobacillus plantarum CRL691. The addition of 90 nmol l-1 of lactocin 705 to cells of L. plantarum dissipated both, the membrane potential (ΔΨ) and the pH gradient (ΔpH). Energized membrane, obtained after the addition of glucose, were more susceptible to lactocin 705 action leading to the immediate release of intracellular K+ and inorganic phosphate. When the role of various ions on sensitive cells were analyzed, only Ca2+ ion exhibited a protective effect against lactocin 705. These data suggest that the presence of a proton motive force (PMF) promotes the interaction of the bacteriocin with the cytoplasmic membrane of energized cells, leading to pore formation which allows for the efflux of ions, thereby ensuring efficient killing of target bacteria.Fil: Castellano, Patricia Haydee. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Raya, Raul Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Vignolo, Graciela Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaElsevier Science2003-08-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/58656Castellano, Patricia Haydee; Raya, Raul Ricardo; Vignolo, Graciela Margarita; Mode of action of lactocin 705, a two-component bacteriocin from Lactobacillus casei CRL705; Elsevier Science; International Journal of Food Microbiology; 85; 1-2; 15-8-2003; 35-430168-16051879-3460CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/S0168-1605(02)00479-8info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168160502004798info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:01Zoai:ri.conicet.gov.ar:11336/58656instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:01.669CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mode of action of lactocin 705, a two-component bacteriocin from Lactobacillus casei CRL705
title Mode of action of lactocin 705, a two-component bacteriocin from Lactobacillus casei CRL705
spellingShingle Mode of action of lactocin 705, a two-component bacteriocin from Lactobacillus casei CRL705
Castellano, Patricia Haydee
Bacteriocin
Lactobacillus
Mode of Action
title_short Mode of action of lactocin 705, a two-component bacteriocin from Lactobacillus casei CRL705
title_full Mode of action of lactocin 705, a two-component bacteriocin from Lactobacillus casei CRL705
title_fullStr Mode of action of lactocin 705, a two-component bacteriocin from Lactobacillus casei CRL705
title_full_unstemmed Mode of action of lactocin 705, a two-component bacteriocin from Lactobacillus casei CRL705
title_sort Mode of action of lactocin 705, a two-component bacteriocin from Lactobacillus casei CRL705
dc.creator.none.fl_str_mv Castellano, Patricia Haydee
Raya, Raul Ricardo
Vignolo, Graciela Margarita
author Castellano, Patricia Haydee
author_facet Castellano, Patricia Haydee
Raya, Raul Ricardo
Vignolo, Graciela Margarita
author_role author
author2 Raya, Raul Ricardo
Vignolo, Graciela Margarita
author2_role author
author
dc.subject.none.fl_str_mv Bacteriocin
Lactobacillus
Mode of Action
topic Bacteriocin
Lactobacillus
Mode of Action
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Lactocin 705 is a bacteriocin whose activity depends on the complementary action of two peptides (705α and 705β) of 33-amino-acid residues each and is produced by Lactobacillus casei CRL705. Biologically active, synthetic lactocin 705 was used to study the mode of action on sensitive cells of Lactobacillus plantarum CRL691. The addition of 90 nmol l-1 of lactocin 705 to cells of L. plantarum dissipated both, the membrane potential (ΔΨ) and the pH gradient (ΔpH). Energized membrane, obtained after the addition of glucose, were more susceptible to lactocin 705 action leading to the immediate release of intracellular K+ and inorganic phosphate. When the role of various ions on sensitive cells were analyzed, only Ca2+ ion exhibited a protective effect against lactocin 705. These data suggest that the presence of a proton motive force (PMF) promotes the interaction of the bacteriocin with the cytoplasmic membrane of energized cells, leading to pore formation which allows for the efflux of ions, thereby ensuring efficient killing of target bacteria.
Fil: Castellano, Patricia Haydee. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Raya, Raul Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Vignolo, Graciela Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description Lactocin 705 is a bacteriocin whose activity depends on the complementary action of two peptides (705α and 705β) of 33-amino-acid residues each and is produced by Lactobacillus casei CRL705. Biologically active, synthetic lactocin 705 was used to study the mode of action on sensitive cells of Lactobacillus plantarum CRL691. The addition of 90 nmol l-1 of lactocin 705 to cells of L. plantarum dissipated both, the membrane potential (ΔΨ) and the pH gradient (ΔpH). Energized membrane, obtained after the addition of glucose, were more susceptible to lactocin 705 action leading to the immediate release of intracellular K+ and inorganic phosphate. When the role of various ions on sensitive cells were analyzed, only Ca2+ ion exhibited a protective effect against lactocin 705. These data suggest that the presence of a proton motive force (PMF) promotes the interaction of the bacteriocin with the cytoplasmic membrane of energized cells, leading to pore formation which allows for the efflux of ions, thereby ensuring efficient killing of target bacteria.
publishDate 2003
dc.date.none.fl_str_mv 2003-08-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/58656
Castellano, Patricia Haydee; Raya, Raul Ricardo; Vignolo, Graciela Margarita; Mode of action of lactocin 705, a two-component bacteriocin from Lactobacillus casei CRL705; Elsevier Science; International Journal of Food Microbiology; 85; 1-2; 15-8-2003; 35-43
0168-1605
1879-3460
CONICET Digital
CONICET
url http://hdl.handle.net/11336/58656
identifier_str_mv Castellano, Patricia Haydee; Raya, Raul Ricardo; Vignolo, Graciela Margarita; Mode of action of lactocin 705, a two-component bacteriocin from Lactobacillus casei CRL705; Elsevier Science; International Journal of Food Microbiology; 85; 1-2; 15-8-2003; 35-43
0168-1605
1879-3460
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/S0168-1605(02)00479-8
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168160502004798
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613127222591488
score 13.070432

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