Role of Pel and Psl polysaccharides in the response of Pseudomonas aeruginosa to environmental challenges: Oxidative stress agents (UVA, H2 O2, sodium hypochlorite) and its competi...

Autores
Grossich, Romina; Lemos Vilches, Martín Oscar; Costa, Cristina Susana; Pezzoni, Magdalena
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pseudomonas aeruginosa is a versatile bacterium capable of adapting to a wide range of stress factors, including solar UVA radiation (400–315 nm). High UVA doses produce lethal effects due to the action of reactive oxygen species. Sublethal UVA doses also induces oxidative damage, but, in addition, it triggers a variety of adaptive responses, including the over-expression of pelA and pslA genes in P. aeruginosa. These genes encode the synthesis of Pel and Psl, which are essential polysaccharides in biofilm formation. The present study analysed the role of Pel and Psl in the adaptive responses gener-ated by exposure to low UVA doses, and their importance in the response to lethal doses of UVA, hydrogen peroxide (H2 O2), and sodium hypochlorite, in both planktonic cells and submerged and air–liquid interface (ALI) biofilms. It also studied the roles of Pel and Psl in P. aeruginosa–Staphylococcus aureus interaction. The results demonstrate that the capacity of sub-lethal UVA exposure to increase cell hydrophobicity and cell attachment and generate cross-protection phenomena in P. aeruginosa depends on the presence of Pel and Psl. The study also shows that Pel and Psl have a key role in the tolerance to lethal doses of UVA radiation, sodium hypochlorite and H2 O2, in both biofilms and planktonic cells. Finally, co-culture assays showed total inhibition of S. aureus growth in presence of P. aeruginosa. This phenomenon depends, at least in part, on the simultaneous presence of Pel and Psl in planktonic cells and biofilms, suggesting a relevant role of these polysaccharides in the interaction between these species.
Fil: Grossich, Romina. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; Argentina
Fil: Lemos Vilches, Martín Oscar. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; Argentina
Fil: Costa, Cristina Susana. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; Argentina
Fil: Pezzoni, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; Argentina. Ministerio de Educación, Cultura, Ciencia y Tecnología. Consejo Interuniversitario Nacional; Argentina
Materia
H2 O2
PEL/PSL
PSEUDOMONAS AERUGINOSA
SODIUM HYPOCHLORITE
UVA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/225158
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/225158
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of Pel and Psl polysaccharides in the response of Pseudomonas aeruginosa to environmental challenges: Oxidative stress agents (UVA, H2 O2, sodium hypochlorite) and its competitor Staphylococcus aureusGrossich, RominaLemos Vilches, Martín OscarCosta, Cristina SusanaPezzoni, MagdalenaH2 O2PEL/PSLPSEUDOMONAS AERUGINOSASODIUM HYPOCHLORITEUVAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Pseudomonas aeruginosa is a versatile bacterium capable of adapting to a wide range of stress factors, including solar UVA radiation (400–315 nm). High UVA doses produce lethal effects due to the action of reactive oxygen species. Sublethal UVA doses also induces oxidative damage, but, in addition, it triggers a variety of adaptive responses, including the over-expression of pelA and pslA genes in P. aeruginosa. These genes encode the synthesis of Pel and Psl, which are essential polysaccharides in biofilm formation. The present study analysed the role of Pel and Psl in the adaptive responses gener-ated by exposure to low UVA doses, and their importance in the response to lethal doses of UVA, hydrogen peroxide (H2 O2), and sodium hypochlorite, in both planktonic cells and submerged and air–liquid interface (ALI) biofilms. It also studied the roles of Pel and Psl in P. aeruginosa–Staphylococcus aureus interaction. The results demonstrate that the capacity of sub-lethal UVA exposure to increase cell hydrophobicity and cell attachment and generate cross-protection phenomena in P. aeruginosa depends on the presence of Pel and Psl. The study also shows that Pel and Psl have a key role in the tolerance to lethal doses of UVA radiation, sodium hypochlorite and H2 O2, in both biofilms and planktonic cells. Finally, co-culture assays showed total inhibition of S. aureus growth in presence of P. aeruginosa. This phenomenon depends, at least in part, on the simultaneous presence of Pel and Psl in planktonic cells and biofilms, suggesting a relevant role of these polysaccharides in the interaction between these species.Fil: Grossich, Romina. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; ArgentinaFil: Lemos Vilches, Martín Oscar. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; ArgentinaFil: Costa, Cristina Susana. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; ArgentinaFil: Pezzoni, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; Argentina. Ministerio de Educación, Cultura, Ciencia y Tecnología. Consejo Interuniversitario Nacional; ArgentinaSociety for General Microbiology2023-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/225158Grossich, Romina; Lemos Vilches, Martín Oscar; Costa, Cristina Susana; Pezzoni, Magdalena; Role of Pel and Psl polysaccharides in the response of Pseudomonas aeruginosa to environmental challenges: Oxidative stress agents (UVA, H2 O2, sodium hypochlorite) and its competitor Staphylococcus aureus; Society for General Microbiology; Microbiology-UK; 169; 2; 2-2023; 1-161350-0872CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.001301info:eu-repo/semantics/altIdentifier/doi/10.1099/mic.0.001301info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:29:20Zoai:ri.conicet.gov.ar:11336/225158instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:29:20.336CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of Pel and Psl polysaccharides in the response of Pseudomonas aeruginosa to environmental challenges: Oxidative stress agents (UVA, H2 O2, sodium hypochlorite) and its competitor Staphylococcus aureus
title Role of Pel and Psl polysaccharides in the response of Pseudomonas aeruginosa to environmental challenges: Oxidative stress agents (UVA, H2 O2, sodium hypochlorite) and its competitor Staphylococcus aureus
spellingShingle Role of Pel and Psl polysaccharides in the response of Pseudomonas aeruginosa to environmental challenges: Oxidative stress agents (UVA, H2 O2, sodium hypochlorite) and its competitor Staphylococcus aureus
Grossich, Romina
H2 O2
PEL/PSL
PSEUDOMONAS AERUGINOSA
SODIUM HYPOCHLORITE
UVA
title_short Role of Pel and Psl polysaccharides in the response of Pseudomonas aeruginosa to environmental challenges: Oxidative stress agents (UVA, H2 O2, sodium hypochlorite) and its competitor Staphylococcus aureus
title_full Role of Pel and Psl polysaccharides in the response of Pseudomonas aeruginosa to environmental challenges: Oxidative stress agents (UVA, H2 O2, sodium hypochlorite) and its competitor Staphylococcus aureus
title_fullStr Role of Pel and Psl polysaccharides in the response of Pseudomonas aeruginosa to environmental challenges: Oxidative stress agents (UVA, H2 O2, sodium hypochlorite) and its competitor Staphylococcus aureus
title_full_unstemmed Role of Pel and Psl polysaccharides in the response of Pseudomonas aeruginosa to environmental challenges: Oxidative stress agents (UVA, H2 O2, sodium hypochlorite) and its competitor Staphylococcus aureus
title_sort Role of Pel and Psl polysaccharides in the response of Pseudomonas aeruginosa to environmental challenges: Oxidative stress agents (UVA, H2 O2, sodium hypochlorite) and its competitor Staphylococcus aureus
dc.creator.none.fl_str_mv Grossich, Romina
Lemos Vilches, Martín Oscar
Costa, Cristina Susana
Pezzoni, Magdalena
author Grossich, Romina
author_facet Grossich, Romina
Lemos Vilches, Martín Oscar
Costa, Cristina Susana
Pezzoni, Magdalena
author_role author
author2 Lemos Vilches, Martín Oscar
Costa, Cristina Susana
Pezzoni, Magdalena
author2_role author
author
author
dc.subject.none.fl_str_mv H2 O2
PEL/PSL
PSEUDOMONAS AERUGINOSA
SODIUM HYPOCHLORITE
UVA
topic H2 O2
PEL/PSL
PSEUDOMONAS AERUGINOSA
SODIUM HYPOCHLORITE
UVA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Pseudomonas aeruginosa is a versatile bacterium capable of adapting to a wide range of stress factors, including solar UVA radiation (400–315 nm). High UVA doses produce lethal effects due to the action of reactive oxygen species. Sublethal UVA doses also induces oxidative damage, but, in addition, it triggers a variety of adaptive responses, including the over-expression of pelA and pslA genes in P. aeruginosa. These genes encode the synthesis of Pel and Psl, which are essential polysaccharides in biofilm formation. The present study analysed the role of Pel and Psl in the adaptive responses gener-ated by exposure to low UVA doses, and their importance in the response to lethal doses of UVA, hydrogen peroxide (H2 O2), and sodium hypochlorite, in both planktonic cells and submerged and air–liquid interface (ALI) biofilms. It also studied the roles of Pel and Psl in P. aeruginosa–Staphylococcus aureus interaction. The results demonstrate that the capacity of sub-lethal UVA exposure to increase cell hydrophobicity and cell attachment and generate cross-protection phenomena in P. aeruginosa depends on the presence of Pel and Psl. The study also shows that Pel and Psl have a key role in the tolerance to lethal doses of UVA radiation, sodium hypochlorite and H2 O2, in both biofilms and planktonic cells. Finally, co-culture assays showed total inhibition of S. aureus growth in presence of P. aeruginosa. This phenomenon depends, at least in part, on the simultaneous presence of Pel and Psl in planktonic cells and biofilms, suggesting a relevant role of these polysaccharides in the interaction between these species.
Fil: Grossich, Romina. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; Argentina
Fil: Lemos Vilches, Martín Oscar. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; Argentina
Fil: Costa, Cristina Susana. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; Argentina
Fil: Pezzoni, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; Argentina. Ministerio de Educación, Cultura, Ciencia y Tecnología. Consejo Interuniversitario Nacional; Argentina
description Pseudomonas aeruginosa is a versatile bacterium capable of adapting to a wide range of stress factors, including solar UVA radiation (400–315 nm). High UVA doses produce lethal effects due to the action of reactive oxygen species. Sublethal UVA doses also induces oxidative damage, but, in addition, it triggers a variety of adaptive responses, including the over-expression of pelA and pslA genes in P. aeruginosa. These genes encode the synthesis of Pel and Psl, which are essential polysaccharides in biofilm formation. The present study analysed the role of Pel and Psl in the adaptive responses gener-ated by exposure to low UVA doses, and their importance in the response to lethal doses of UVA, hydrogen peroxide (H2 O2), and sodium hypochlorite, in both planktonic cells and submerged and air–liquid interface (ALI) biofilms. It also studied the roles of Pel and Psl in P. aeruginosa–Staphylococcus aureus interaction. The results demonstrate that the capacity of sub-lethal UVA exposure to increase cell hydrophobicity and cell attachment and generate cross-protection phenomena in P. aeruginosa depends on the presence of Pel and Psl. The study also shows that Pel and Psl have a key role in the tolerance to lethal doses of UVA radiation, sodium hypochlorite and H2 O2, in both biofilms and planktonic cells. Finally, co-culture assays showed total inhibition of S. aureus growth in presence of P. aeruginosa. This phenomenon depends, at least in part, on the simultaneous presence of Pel and Psl in planktonic cells and biofilms, suggesting a relevant role of these polysaccharides in the interaction between these species.
publishDate 2023
dc.date.none.fl_str_mv 2023-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/225158
Grossich, Romina; Lemos Vilches, Martín Oscar; Costa, Cristina Susana; Pezzoni, Magdalena; Role of Pel and Psl polysaccharides in the response of Pseudomonas aeruginosa to environmental challenges: Oxidative stress agents (UVA, H2 O2, sodium hypochlorite) and its competitor Staphylococcus aureus; Society for General Microbiology; Microbiology-UK; 169; 2; 2-2023; 1-16
1350-0872
CONICET Digital
CONICET
url http://hdl.handle.net/11336/225158
identifier_str_mv Grossich, Romina; Lemos Vilches, Martín Oscar; Costa, Cristina Susana; Pezzoni, Magdalena; Role of Pel and Psl polysaccharides in the response of Pseudomonas aeruginosa to environmental challenges: Oxidative stress agents (UVA, H2 O2, sodium hypochlorite) and its competitor Staphylococcus aureus; Society for General Microbiology; Microbiology-UK; 169; 2; 2-2023; 1-16
1350-0872
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.001301
info:eu-repo/semantics/altIdentifier/doi/10.1099/mic.0.001301
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for General Microbiology
publisher.none.fl_str_mv Society for General Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.046268

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