Gametogenesis and reproductive dynamics of scinax acuminatus (Anura: Hylidae): Morphological, histological and immunohistochemical analysis

Autores
Olea, Gabriela Beatriz; Cheij, Esteban Omar; Cuzziol Boccioni, Ana Paula; Rodriguez, Florencia Evelyn; Céspedez, Jorge; Lombardo, Daniel Marcelo
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To characterize the reproductive biology of Scinax acuminatus and contribute to the natural history of this species, the morphology of the reproductive system of males and females was analyzed at anatomical, histological and immunohistochemical levels. The individuals were collected fortnightly between August and December (2016) and January to December (2018). The anatomy of the reproductive system was analyzed in a stereoscopic microscope, and histological preparations staining with hematoxylin-eosin and Masson’s trichromic, PAS and Coomassie Blue was performed as well. To characterize the meiotic-active cells in the testes, immunostaining with the PCNA proliferation protein was performed. There were found females with ovaries with oocytes in different stages of maturity and post-ovulatory females. Males presented continuous spermatogenesis, which could be confirmed by the immunostaining of PCNA in spermatogonia during the cycle. The results of this work serve as a basis for the characterization of the reproductive cycle in S. acuminatus and provide background information on the analysis of spermatogenic activity by IHQ from the study of the immunodetection of the PCNA cell proliferation protein. Future studies will focus on the evaluation of cell death processes during the reproductive cycle in the studied species to compare with those obtained in terms of cell proliferation.
Fil: Olea, Gabriela Beatriz. Universidad Nacional del Nordeste. Facultad de Medicina. Laboratorio de Investigaciones Bioquímicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cheij, Esteban Omar. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina
Fil: Cuzziol Boccioni, Ana Paula. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodriguez, Florencia Evelyn. Universidad Nacional del Nordeste. Facultad de Medicina. Laboratorio de Investigaciones Bioquímicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Céspedez, Jorge. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina
Fil: Lombardo, Daniel Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
AMPHIBIANS
OVARIES
REPRODUCTION
TESTICLES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/137212
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Gametogenesis and reproductive dynamics of scinax acuminatus (Anura: Hylidae): Morphological, histological and immunohistochemical analysisOlea, Gabriela BeatrizCheij, Esteban OmarCuzziol Boccioni, Ana PaulaRodriguez, Florencia EvelynCéspedez, JorgeLombardo, Daniel MarceloAMPHIBIANSOVARIESREPRODUCTIONTESTICLEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1To characterize the reproductive biology of Scinax acuminatus and contribute to the natural history of this species, the morphology of the reproductive system of males and females was analyzed at anatomical, histological and immunohistochemical levels. The individuals were collected fortnightly between August and December (2016) and January to December (2018). The anatomy of the reproductive system was analyzed in a stereoscopic microscope, and histological preparations staining with hematoxylin-eosin and Masson’s trichromic, PAS and Coomassie Blue was performed as well. To characterize the meiotic-active cells in the testes, immunostaining with the PCNA proliferation protein was performed. There were found females with ovaries with oocytes in different stages of maturity and post-ovulatory females. Males presented continuous spermatogenesis, which could be confirmed by the immunostaining of PCNA in spermatogonia during the cycle. The results of this work serve as a basis for the characterization of the reproductive cycle in S. acuminatus and provide background information on the analysis of spermatogenic activity by IHQ from the study of the immunodetection of the PCNA cell proliferation protein. Future studies will focus on the evaluation of cell death processes during the reproductive cycle in the studied species to compare with those obtained in terms of cell proliferation.Fil: Olea, Gabriela Beatriz. Universidad Nacional del Nordeste. Facultad de Medicina. Laboratorio de Investigaciones Bioquímicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cheij, Esteban Omar. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; ArgentinaFil: Cuzziol Boccioni, Ana Paula. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rodriguez, Florencia Evelyn. Universidad Nacional del Nordeste. Facultad de Medicina. Laboratorio de Investigaciones Bioquímicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Céspedez, Jorge. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; ArgentinaFil: Lombardo, Daniel Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaAcademia Brasileira de Ciencias2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/137212Olea, Gabriela Beatriz; Cheij, Esteban Omar; Cuzziol Boccioni, Ana Paula; Rodriguez, Florencia Evelyn; Céspedez, Jorge; et al.; Gametogenesis and reproductive dynamics of scinax acuminatus (Anura: Hylidae): Morphological, histological and immunohistochemical analysis; Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 93; 2; 2021; 1-110001-37651678-2690CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1590/0001-3765202120190841info:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/j/aabc/a/qV3hCQYChRTsP3Z6sSSRJVf/?lang=eninfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:44Zoai:ri.conicet.gov.ar:11336/137212instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:44.369CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Gametogenesis and reproductive dynamics of scinax acuminatus (Anura: Hylidae): Morphological, histological and immunohistochemical analysis
title Gametogenesis and reproductive dynamics of scinax acuminatus (Anura: Hylidae): Morphological, histological and immunohistochemical analysis
spellingShingle Gametogenesis and reproductive dynamics of scinax acuminatus (Anura: Hylidae): Morphological, histological and immunohistochemical analysis
Olea, Gabriela Beatriz
AMPHIBIANS
OVARIES
REPRODUCTION
TESTICLES
title_short Gametogenesis and reproductive dynamics of scinax acuminatus (Anura: Hylidae): Morphological, histological and immunohistochemical analysis
title_full Gametogenesis and reproductive dynamics of scinax acuminatus (Anura: Hylidae): Morphological, histological and immunohistochemical analysis
title_fullStr Gametogenesis and reproductive dynamics of scinax acuminatus (Anura: Hylidae): Morphological, histological and immunohistochemical analysis
title_full_unstemmed Gametogenesis and reproductive dynamics of scinax acuminatus (Anura: Hylidae): Morphological, histological and immunohistochemical analysis
title_sort Gametogenesis and reproductive dynamics of scinax acuminatus (Anura: Hylidae): Morphological, histological and immunohistochemical analysis
dc.creator.none.fl_str_mv Olea, Gabriela Beatriz
Cheij, Esteban Omar
Cuzziol Boccioni, Ana Paula
Rodriguez, Florencia Evelyn
Céspedez, Jorge
Lombardo, Daniel Marcelo
author Olea, Gabriela Beatriz
author_facet Olea, Gabriela Beatriz
Cheij, Esteban Omar
Cuzziol Boccioni, Ana Paula
Rodriguez, Florencia Evelyn
Céspedez, Jorge
Lombardo, Daniel Marcelo
author_role author
author2 Cheij, Esteban Omar
Cuzziol Boccioni, Ana Paula
Rodriguez, Florencia Evelyn
Céspedez, Jorge
Lombardo, Daniel Marcelo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv AMPHIBIANS
OVARIES
REPRODUCTION
TESTICLES
topic AMPHIBIANS
OVARIES
REPRODUCTION
TESTICLES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv To characterize the reproductive biology of Scinax acuminatus and contribute to the natural history of this species, the morphology of the reproductive system of males and females was analyzed at anatomical, histological and immunohistochemical levels. The individuals were collected fortnightly between August and December (2016) and January to December (2018). The anatomy of the reproductive system was analyzed in a stereoscopic microscope, and histological preparations staining with hematoxylin-eosin and Masson’s trichromic, PAS and Coomassie Blue was performed as well. To characterize the meiotic-active cells in the testes, immunostaining with the PCNA proliferation protein was performed. There were found females with ovaries with oocytes in different stages of maturity and post-ovulatory females. Males presented continuous spermatogenesis, which could be confirmed by the immunostaining of PCNA in spermatogonia during the cycle. The results of this work serve as a basis for the characterization of the reproductive cycle in S. acuminatus and provide background information on the analysis of spermatogenic activity by IHQ from the study of the immunodetection of the PCNA cell proliferation protein. Future studies will focus on the evaluation of cell death processes during the reproductive cycle in the studied species to compare with those obtained in terms of cell proliferation.
Fil: Olea, Gabriela Beatriz. Universidad Nacional del Nordeste. Facultad de Medicina. Laboratorio de Investigaciones Bioquímicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cheij, Esteban Omar. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina
Fil: Cuzziol Boccioni, Ana Paula. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodriguez, Florencia Evelyn. Universidad Nacional del Nordeste. Facultad de Medicina. Laboratorio de Investigaciones Bioquímicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Céspedez, Jorge. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina
Fil: Lombardo, Daniel Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description To characterize the reproductive biology of Scinax acuminatus and contribute to the natural history of this species, the morphology of the reproductive system of males and females was analyzed at anatomical, histological and immunohistochemical levels. The individuals were collected fortnightly between August and December (2016) and January to December (2018). The anatomy of the reproductive system was analyzed in a stereoscopic microscope, and histological preparations staining with hematoxylin-eosin and Masson’s trichromic, PAS and Coomassie Blue was performed as well. To characterize the meiotic-active cells in the testes, immunostaining with the PCNA proliferation protein was performed. There were found females with ovaries with oocytes in different stages of maturity and post-ovulatory females. Males presented continuous spermatogenesis, which could be confirmed by the immunostaining of PCNA in spermatogonia during the cycle. The results of this work serve as a basis for the characterization of the reproductive cycle in S. acuminatus and provide background information on the analysis of spermatogenic activity by IHQ from the study of the immunodetection of the PCNA cell proliferation protein. Future studies will focus on the evaluation of cell death processes during the reproductive cycle in the studied species to compare with those obtained in terms of cell proliferation.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/137212
Olea, Gabriela Beatriz; Cheij, Esteban Omar; Cuzziol Boccioni, Ana Paula; Rodriguez, Florencia Evelyn; Céspedez, Jorge; et al.; Gametogenesis and reproductive dynamics of scinax acuminatus (Anura: Hylidae): Morphological, histological and immunohistochemical analysis; Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 93; 2; 2021; 1-11
0001-3765
1678-2690
CONICET Digital
CONICET
url http://hdl.handle.net/11336/137212
identifier_str_mv Olea, Gabriela Beatriz; Cheij, Esteban Omar; Cuzziol Boccioni, Ana Paula; Rodriguez, Florencia Evelyn; Céspedez, Jorge; et al.; Gametogenesis and reproductive dynamics of scinax acuminatus (Anura: Hylidae): Morphological, histological and immunohistochemical analysis; Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 93; 2; 2021; 1-11
0001-3765
1678-2690
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1590/0001-3765202120190841
info:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/j/aabc/a/qV3hCQYChRTsP3Z6sSSRJVf/?lang=en
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academia Brasileira de Ciencias
publisher.none.fl_str_mv Academia Brasileira de Ciencias
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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