c-Fos-activated synthesis of nuclear phosphatidylinositol 4,5-bisphosphate[PtdIns(4,5)P2] promotes global transcriptional changes

Autores
Ferrero, Gabriel Orlando; Renner, Marianne L.; Gil, German Alejandro; Rodríguez, Lucía; Caputto, Beatriz Leonor
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
c-Fos is a well-recognized member of the AP-1 (activator protein-1) family of transcription factors. In addition to this canonical activity, we previously showed that cytoplasmic c-Fos activates phospholipid synthesis through a mechanism independent of its genomic AP-1 activity. c-Fos associates with particular enzymes of the lipid synthesis pathway at the endoplasmic reticulum and increases the Vmax of the reactions without modifying the Km values. This lipid synthesis activation is associated with events of differentiation and proliferation that require high rates of membrane biogenesis. Since lipid synthesis also occurs in the nucleus, and different phospholipids have been assigned transcription regulatory functions, in the present study we examine if c-Fos also acts as a regulator of phospholipid synthesis in the nucleus. Furthermore, we examine if c-Fos modulates transcription through its phospholipid synthesis activator capacity. We show that nuclear-localized c-Fos associates with and activates PI4P5K (phosphatidylinositol-4-monophosphate 5-kinase), but not with PI4KIIIβ (type IIIβ phosphatidylinositol 4-kinase) thus promoting PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) formation, which, in turn, promotes transcriptional changes. We propose c-Fos as a key regulator of nuclear PtdIns(4,5)P2 synthesis in response to growth signals that results in c-Fos-dependent transcriptional changes promoted by the newly synthesized lipids.
Fil: Ferrero, Gabriel Orlando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Renner, Marianne L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Gil, German Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Rodríguez, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Caputto, Beatriz Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Materia
Activator Protein-1 (Ap-1) Transcription Factor
C-Fos-Dependent Phospholipid Synthesis
Nuclear Phospholipidsynthesis
Phosphatidylinositol-4,5-Bisphosphate [Ptdins(4,5)P2]
Phosphatidylinositol4-Monophosphate5-Kinase(Pi4p5k)
Transcription Regulation.
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/32111
Acceder
id CONICETDig_f829c5e39999dac853935a0081682d68
oai_identifier_str oai:ri.conicet.gov.ar:11336/32111
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling c-Fos-activated synthesis of nuclear phosphatidylinositol 4,5-bisphosphate[PtdIns(4,5)P2] promotes global transcriptional changesFerrero, Gabriel OrlandoRenner, Marianne L.Gil, German AlejandroRodríguez, LucíaCaputto, Beatriz LeonorActivator Protein-1 (Ap-1) Transcription FactorC-Fos-Dependent Phospholipid SynthesisNuclear PhospholipidsynthesisPhosphatidylinositol-4,5-Bisphosphate [Ptdins(4,5)P2]Phosphatidylinositol4-Monophosphate5-Kinase(Pi4p5k)Transcription Regulation.https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1c-Fos is a well-recognized member of the AP-1 (activator protein-1) family of transcription factors. In addition to this canonical activity, we previously showed that cytoplasmic c-Fos activates phospholipid synthesis through a mechanism independent of its genomic AP-1 activity. c-Fos associates with particular enzymes of the lipid synthesis pathway at the endoplasmic reticulum and increases the Vmax of the reactions without modifying the Km values. This lipid synthesis activation is associated with events of differentiation and proliferation that require high rates of membrane biogenesis. Since lipid synthesis also occurs in the nucleus, and different phospholipids have been assigned transcription regulatory functions, in the present study we examine if c-Fos also acts as a regulator of phospholipid synthesis in the nucleus. Furthermore, we examine if c-Fos modulates transcription through its phospholipid synthesis activator capacity. We show that nuclear-localized c-Fos associates with and activates PI4P5K (phosphatidylinositol-4-monophosphate 5-kinase), but not with PI4KIIIβ (type IIIβ phosphatidylinositol 4-kinase) thus promoting PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) formation, which, in turn, promotes transcriptional changes. We propose c-Fos as a key regulator of nuclear PtdIns(4,5)P2 synthesis in response to growth signals that results in c-Fos-dependent transcriptional changes promoted by the newly synthesized lipids.Fil: Ferrero, Gabriel Orlando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Renner, Marianne L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Gil, German Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Rodríguez, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Caputto, Beatriz Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaPortland Press2014-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32111Caputto, Beatriz Leonor; Rodríguez, Lucía; Gil, German Alejandro; Renner, Marianne L.; Ferrero, Gabriel Orlando; c-Fos-activated synthesis of nuclear phosphatidylinositol 4,5-bisphosphate[PtdIns(4,5)P2] promotes global transcriptional changes; Portland Press; Biochemical Journal; 461; 3; 8-2014; 521-5300264-6021CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1042/BJ20131376info:eu-repo/semantics/altIdentifier/url/http://www.biochemj.org/content/461/3/521info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:30:32Zoai:ri.conicet.gov.ar:11336/32111instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:30:32.445CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv c-Fos-activated synthesis of nuclear phosphatidylinositol 4,5-bisphosphate[PtdIns(4,5)P2] promotes global transcriptional changes
title c-Fos-activated synthesis of nuclear phosphatidylinositol 4,5-bisphosphate[PtdIns(4,5)P2] promotes global transcriptional changes
spellingShingle c-Fos-activated synthesis of nuclear phosphatidylinositol 4,5-bisphosphate[PtdIns(4,5)P2] promotes global transcriptional changes
Ferrero, Gabriel Orlando
Activator Protein-1 (Ap-1) Transcription Factor
C-Fos-Dependent Phospholipid Synthesis
Nuclear Phospholipidsynthesis
Phosphatidylinositol-4,5-Bisphosphate [Ptdins(4,5)P2]
Phosphatidylinositol4-Monophosphate5-Kinase(Pi4p5k)
Transcription Regulation.
title_short c-Fos-activated synthesis of nuclear phosphatidylinositol 4,5-bisphosphate[PtdIns(4,5)P2] promotes global transcriptional changes
title_full c-Fos-activated synthesis of nuclear phosphatidylinositol 4,5-bisphosphate[PtdIns(4,5)P2] promotes global transcriptional changes
title_fullStr c-Fos-activated synthesis of nuclear phosphatidylinositol 4,5-bisphosphate[PtdIns(4,5)P2] promotes global transcriptional changes
title_full_unstemmed c-Fos-activated synthesis of nuclear phosphatidylinositol 4,5-bisphosphate[PtdIns(4,5)P2] promotes global transcriptional changes
title_sort c-Fos-activated synthesis of nuclear phosphatidylinositol 4,5-bisphosphate[PtdIns(4,5)P2] promotes global transcriptional changes
dc.creator.none.fl_str_mv Ferrero, Gabriel Orlando
Renner, Marianne L.
Gil, German Alejandro
Rodríguez, Lucía
Caputto, Beatriz Leonor
author Ferrero, Gabriel Orlando
author_facet Ferrero, Gabriel Orlando
Renner, Marianne L.
Gil, German Alejandro
Rodríguez, Lucía
Caputto, Beatriz Leonor
author_role author
author2 Renner, Marianne L.
Gil, German Alejandro
Rodríguez, Lucía
Caputto, Beatriz Leonor
author2_role author
author
author
author
dc.subject.none.fl_str_mv Activator Protein-1 (Ap-1) Transcription Factor
C-Fos-Dependent Phospholipid Synthesis
Nuclear Phospholipidsynthesis
Phosphatidylinositol-4,5-Bisphosphate [Ptdins(4,5)P2]
Phosphatidylinositol4-Monophosphate5-Kinase(Pi4p5k)
Transcription Regulation.
topic Activator Protein-1 (Ap-1) Transcription Factor
C-Fos-Dependent Phospholipid Synthesis
Nuclear Phospholipidsynthesis
Phosphatidylinositol-4,5-Bisphosphate [Ptdins(4,5)P2]
Phosphatidylinositol4-Monophosphate5-Kinase(Pi4p5k)
Transcription Regulation.
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv c-Fos is a well-recognized member of the AP-1 (activator protein-1) family of transcription factors. In addition to this canonical activity, we previously showed that cytoplasmic c-Fos activates phospholipid synthesis through a mechanism independent of its genomic AP-1 activity. c-Fos associates with particular enzymes of the lipid synthesis pathway at the endoplasmic reticulum and increases the Vmax of the reactions without modifying the Km values. This lipid synthesis activation is associated with events of differentiation and proliferation that require high rates of membrane biogenesis. Since lipid synthesis also occurs in the nucleus, and different phospholipids have been assigned transcription regulatory functions, in the present study we examine if c-Fos also acts as a regulator of phospholipid synthesis in the nucleus. Furthermore, we examine if c-Fos modulates transcription through its phospholipid synthesis activator capacity. We show that nuclear-localized c-Fos associates with and activates PI4P5K (phosphatidylinositol-4-monophosphate 5-kinase), but not with PI4KIIIβ (type IIIβ phosphatidylinositol 4-kinase) thus promoting PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) formation, which, in turn, promotes transcriptional changes. We propose c-Fos as a key regulator of nuclear PtdIns(4,5)P2 synthesis in response to growth signals that results in c-Fos-dependent transcriptional changes promoted by the newly synthesized lipids.
Fil: Ferrero, Gabriel Orlando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Renner, Marianne L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Gil, German Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Rodríguez, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Caputto, Beatriz Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
description c-Fos is a well-recognized member of the AP-1 (activator protein-1) family of transcription factors. In addition to this canonical activity, we previously showed that cytoplasmic c-Fos activates phospholipid synthesis through a mechanism independent of its genomic AP-1 activity. c-Fos associates with particular enzymes of the lipid synthesis pathway at the endoplasmic reticulum and increases the Vmax of the reactions without modifying the Km values. This lipid synthesis activation is associated with events of differentiation and proliferation that require high rates of membrane biogenesis. Since lipid synthesis also occurs in the nucleus, and different phospholipids have been assigned transcription regulatory functions, in the present study we examine if c-Fos also acts as a regulator of phospholipid synthesis in the nucleus. Furthermore, we examine if c-Fos modulates transcription through its phospholipid synthesis activator capacity. We show that nuclear-localized c-Fos associates with and activates PI4P5K (phosphatidylinositol-4-monophosphate 5-kinase), but not with PI4KIIIβ (type IIIβ phosphatidylinositol 4-kinase) thus promoting PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) formation, which, in turn, promotes transcriptional changes. We propose c-Fos as a key regulator of nuclear PtdIns(4,5)P2 synthesis in response to growth signals that results in c-Fos-dependent transcriptional changes promoted by the newly synthesized lipids.
publishDate 2014
dc.date.none.fl_str_mv 2014-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/32111
Caputto, Beatriz Leonor; Rodríguez, Lucía; Gil, German Alejandro; Renner, Marianne L.; Ferrero, Gabriel Orlando; c-Fos-activated synthesis of nuclear phosphatidylinositol 4,5-bisphosphate[PtdIns(4,5)P2] promotes global transcriptional changes; Portland Press; Biochemical Journal; 461; 3; 8-2014; 521-530
0264-6021
CONICET Digital
CONICET
url http://hdl.handle.net/11336/32111
identifier_str_mv Caputto, Beatriz Leonor; Rodríguez, Lucía; Gil, German Alejandro; Renner, Marianne L.; Ferrero, Gabriel Orlando; c-Fos-activated synthesis of nuclear phosphatidylinositol 4,5-bisphosphate[PtdIns(4,5)P2] promotes global transcriptional changes; Portland Press; Biochemical Journal; 461; 3; 8-2014; 521-530
0264-6021
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1042/BJ20131376
info:eu-repo/semantics/altIdentifier/url/http://www.biochemj.org/content/461/3/521
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Portland Press
publisher.none.fl_str_mv Portland Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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