Vesicular fractions of sunflower apoplastic fluids are associated with potential exosome marker proteins

Autores
Regente, Mariana Clelia; Corti Monzón, Georgina de la Paz; Maldonado, Ana Maria; Pinedo, Marcela Lilian; Jorrin, Jesus; de la Canal, Laura
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Based on the presence of phospholipids in the extracellular fluids (EFs) of sunflower seeds, we have hypothesized on the existence of vesicles in the apoplastic compartment of plants. Ultracentrifugation of sunflower EF allowed the isolation of particles of 50-200 nm with apparent membrane organization. A small GTPase Rab was putatively identified in this vesicular fraction. Since Rab proteins are involved in vesicular traffic and their presence in exosomes from animal fluids has been demonstrated, evidence presented here supports the existence of exosome-like vesicles in apoplastic fluids of sunflower. Their putative contribution to intercellular communication in plants is discussed.
Fil: Regente, Mariana Clelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Corti Monzón, Georgina de la Paz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Maldonado, Ana Maria. Universidad de Córdoba; España
Fil: Pinedo, Marcela Lilian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Jorrin, Jesus. Universidad de Córdoba; España
Fil: de la Canal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Materia
EXOSOME
EXTRACELLULAR
GTPASE
HELIANTHUS
PHOSPHOLIPIDS
RAB
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/133759
Acceder
id CONICETDig_f7d751f4bff9091f0f12b70a96982c7e
oai_identifier_str oai:ri.conicet.gov.ar:11336/133759
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Vesicular fractions of sunflower apoplastic fluids are associated with potential exosome marker proteinsRegente, Mariana CleliaCorti Monzón, Georgina de la PazMaldonado, Ana MariaPinedo, Marcela LilianJorrin, Jesusde la Canal, LauraEXOSOMEEXTRACELLULARGTPASEHELIANTHUSPHOSPHOLIPIDSRABhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Based on the presence of phospholipids in the extracellular fluids (EFs) of sunflower seeds, we have hypothesized on the existence of vesicles in the apoplastic compartment of plants. Ultracentrifugation of sunflower EF allowed the isolation of particles of 50-200 nm with apparent membrane organization. A small GTPase Rab was putatively identified in this vesicular fraction. Since Rab proteins are involved in vesicular traffic and their presence in exosomes from animal fluids has been demonstrated, evidence presented here supports the existence of exosome-like vesicles in apoplastic fluids of sunflower. Their putative contribution to intercellular communication in plants is discussed.Fil: Regente, Mariana Clelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Corti Monzón, Georgina de la Paz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Maldonado, Ana Maria. Universidad de Córdoba; EspañaFil: Pinedo, Marcela Lilian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Jorrin, Jesus. Universidad de Córdoba; EspañaFil: de la Canal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaElsevier Science2009-09-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/133759Regente, Mariana Clelia; Corti Monzón, Georgina de la Paz; Maldonado, Ana Maria; Pinedo, Marcela Lilian; Jorrin, Jesus; et al.; Vesicular fractions of sunflower apoplastic fluids are associated with potential exosome marker proteins; Elsevier Science; FEBS Letters; 583; 20; 29-9-2009; 3363-33660014-5793CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.febslet.2009.09.041info:eu-repo/semantics/altIdentifier/url/https://febs.onlinelibrary.wiley.com/doi/full/10.1016/j.febslet.2009.09.041info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:29Zoai:ri.conicet.gov.ar:11336/133759instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:30.215CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Vesicular fractions of sunflower apoplastic fluids are associated with potential exosome marker proteins
title Vesicular fractions of sunflower apoplastic fluids are associated with potential exosome marker proteins
spellingShingle Vesicular fractions of sunflower apoplastic fluids are associated with potential exosome marker proteins
Regente, Mariana Clelia
EXOSOME
EXTRACELLULAR
GTPASE
HELIANTHUS
PHOSPHOLIPIDS
RAB
title_short Vesicular fractions of sunflower apoplastic fluids are associated with potential exosome marker proteins
title_full Vesicular fractions of sunflower apoplastic fluids are associated with potential exosome marker proteins
title_fullStr Vesicular fractions of sunflower apoplastic fluids are associated with potential exosome marker proteins
title_full_unstemmed Vesicular fractions of sunflower apoplastic fluids are associated with potential exosome marker proteins
title_sort Vesicular fractions of sunflower apoplastic fluids are associated with potential exosome marker proteins
dc.creator.none.fl_str_mv Regente, Mariana Clelia
Corti Monzón, Georgina de la Paz
Maldonado, Ana Maria
Pinedo, Marcela Lilian
Jorrin, Jesus
de la Canal, Laura
author Regente, Mariana Clelia
author_facet Regente, Mariana Clelia
Corti Monzón, Georgina de la Paz
Maldonado, Ana Maria
Pinedo, Marcela Lilian
Jorrin, Jesus
de la Canal, Laura
author_role author
author2 Corti Monzón, Georgina de la Paz
Maldonado, Ana Maria
Pinedo, Marcela Lilian
Jorrin, Jesus
de la Canal, Laura
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv EXOSOME
EXTRACELLULAR
GTPASE
HELIANTHUS
PHOSPHOLIPIDS
RAB
topic EXOSOME
EXTRACELLULAR
GTPASE
HELIANTHUS
PHOSPHOLIPIDS
RAB
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Based on the presence of phospholipids in the extracellular fluids (EFs) of sunflower seeds, we have hypothesized on the existence of vesicles in the apoplastic compartment of plants. Ultracentrifugation of sunflower EF allowed the isolation of particles of 50-200 nm with apparent membrane organization. A small GTPase Rab was putatively identified in this vesicular fraction. Since Rab proteins are involved in vesicular traffic and their presence in exosomes from animal fluids has been demonstrated, evidence presented here supports the existence of exosome-like vesicles in apoplastic fluids of sunflower. Their putative contribution to intercellular communication in plants is discussed.
Fil: Regente, Mariana Clelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Corti Monzón, Georgina de la Paz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Maldonado, Ana Maria. Universidad de Córdoba; España
Fil: Pinedo, Marcela Lilian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Jorrin, Jesus. Universidad de Córdoba; España
Fil: de la Canal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
description Based on the presence of phospholipids in the extracellular fluids (EFs) of sunflower seeds, we have hypothesized on the existence of vesicles in the apoplastic compartment of plants. Ultracentrifugation of sunflower EF allowed the isolation of particles of 50-200 nm with apparent membrane organization. A small GTPase Rab was putatively identified in this vesicular fraction. Since Rab proteins are involved in vesicular traffic and their presence in exosomes from animal fluids has been demonstrated, evidence presented here supports the existence of exosome-like vesicles in apoplastic fluids of sunflower. Their putative contribution to intercellular communication in plants is discussed.
publishDate 2009
dc.date.none.fl_str_mv 2009-09-29
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/133759
Regente, Mariana Clelia; Corti Monzón, Georgina de la Paz; Maldonado, Ana Maria; Pinedo, Marcela Lilian; Jorrin, Jesus; et al.; Vesicular fractions of sunflower apoplastic fluids are associated with potential exosome marker proteins; Elsevier Science; FEBS Letters; 583; 20; 29-9-2009; 3363-3366
0014-5793
CONICET Digital
CONICET
url http://hdl.handle.net/11336/133759
identifier_str_mv Regente, Mariana Clelia; Corti Monzón, Georgina de la Paz; Maldonado, Ana Maria; Pinedo, Marcela Lilian; Jorrin, Jesus; et al.; Vesicular fractions of sunflower apoplastic fluids are associated with potential exosome marker proteins; Elsevier Science; FEBS Letters; 583; 20; 29-9-2009; 3363-3366
0014-5793
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.febslet.2009.09.041
info:eu-repo/semantics/altIdentifier/url/https://febs.onlinelibrary.wiley.com/doi/full/10.1016/j.febslet.2009.09.041
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268927237816320
score 13.13397

Publicaciones similares