Inmuno-spin trapping from biochemistry to medicine: advances, challenges, and pitfalls. Focus on protein-centered radicals

Autores
Gomez-Mejiba, Sandra Esther; Zhai, Zili; Della Vedova, Maria Cecilia; Muñoz, Marcos David; Chatterjee, Saurabh; Towner, Rheal A.; Hensley, Kenneth; Floyd, Robert A.; Mason, Ronald P.; Ramirez, Dario
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Immuno-spin trapping (IST) is based on the reaction of a spin trap with a free radical to form a stable nitrone adduct, followed by the use of antibodies, rather than traditional electron paramagnetic resonance spectroscopy, to detect the nitrone adduct. IST has been successfully applied to mechanistic in vitro studies, and recently, macromolecule-centered radicals have been detected in models of drug-induced agranulocytosis, hepatotoxicity, cardiotoxicity, and ischemia/reperfusion, as well as in models of neurological, metabolic and immunological diseases. Scope of the review: To critically evaluate advances, challenges, and pitfalls as well as the scientific opportunities of IST as applied to the study of protein-centered free radicals generated in stressed organelles, cells, tissues and animal models of disease and exposure. Major conclusions: Because the spin trap has to be present at high enough concentrations in the microenvironment where the radical is formed, the possible effects of the spin trap on gene expression, metabolism and cell physiology have to be considered in the use of IST and in the interpretation of results. These factors have not yet been thoroughly dealt with in the literature. General significance: The identification of radicalized proteins during cell/tissue response to stressors will help define their role in the complex cellular response to stressors and pathogenesis; however, the fidelity of spin trapping/immuno-detection and the effects of the spin trap on the biological system should be considered.
Fil: Gomez-Mejiba, Sandra Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; Argentina
Fil: Zhai, Zili. University Of Chicago; Estados Unidos
Fil: Della Vedova, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; Argentina
Fil: Muñoz, Marcos David. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; Argentina
Fil: Chatterjee, Saurabh. University of South Carolina; Estados Unidos
Fil: Towner, Rheal A.. Oklahoma Medical Research Foundation; Estados Unidos
Fil: Hensley, Kenneth. University of Toledo; Estados Unidos
Fil: Floyd, Robert A.. Oklahoma Medical Research Foundation; Estados Unidos
Fil: Mason, Ronald P.. National Institute of Environmental Health Sciences; Estados Unidos
Fil: Ramirez, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Area de Biología Molecular; Argentina
Materia
ANTI-DMPO
DISEASE/EXPOSURE MODEL
IMMUNO-SPIN TRAPPING
PROTEIN RADICAL
REACTIVE CHEMICAL SPECIES
SPIN TRAP
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14656
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/14656
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Inmuno-spin trapping from biochemistry to medicine: advances, challenges, and pitfalls. Focus on protein-centered radicalsGomez-Mejiba, Sandra EstherZhai, ZiliDella Vedova, Maria CeciliaMuñoz, Marcos DavidChatterjee, SaurabhTowner, Rheal A.Hensley, KennethFloyd, Robert A.Mason, Ronald P.Ramirez, DarioANTI-DMPODISEASE/EXPOSURE MODELIMMUNO-SPIN TRAPPINGPROTEIN RADICALREACTIVE CHEMICAL SPECIESSPIN TRAPhttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3Background: Immuno-spin trapping (IST) is based on the reaction of a spin trap with a free radical to form a stable nitrone adduct, followed by the use of antibodies, rather than traditional electron paramagnetic resonance spectroscopy, to detect the nitrone adduct. IST has been successfully applied to mechanistic in vitro studies, and recently, macromolecule-centered radicals have been detected in models of drug-induced agranulocytosis, hepatotoxicity, cardiotoxicity, and ischemia/reperfusion, as well as in models of neurological, metabolic and immunological diseases. Scope of the review: To critically evaluate advances, challenges, and pitfalls as well as the scientific opportunities of IST as applied to the study of protein-centered free radicals generated in stressed organelles, cells, tissues and animal models of disease and exposure. Major conclusions: Because the spin trap has to be present at high enough concentrations in the microenvironment where the radical is formed, the possible effects of the spin trap on gene expression, metabolism and cell physiology have to be considered in the use of IST and in the interpretation of results. These factors have not yet been thoroughly dealt with in the literature. General significance: The identification of radicalized proteins during cell/tissue response to stressors will help define their role in the complex cellular response to stressors and pathogenesis; however, the fidelity of spin trapping/immuno-detection and the effects of the spin trap on the biological system should be considered.Fil: Gomez-Mejiba, Sandra Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; ArgentinaFil: Zhai, Zili. University Of Chicago; Estados UnidosFil: Della Vedova, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; ArgentinaFil: Muñoz, Marcos David. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; ArgentinaFil: Chatterjee, Saurabh. University of South Carolina; Estados UnidosFil: Towner, Rheal A.. Oklahoma Medical Research Foundation; Estados UnidosFil: Hensley, Kenneth. University of Toledo; Estados UnidosFil: Floyd, Robert A.. Oklahoma Medical Research Foundation; Estados UnidosFil: Mason, Ronald P.. National Institute of Environmental Health Sciences; Estados UnidosFil: Ramirez, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Area de Biología Molecular; ArgentinaElsevier Science2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14656Gomez-Mejiba, Sandra Esther; Zhai, Zili; Della Vedova, Maria Cecilia; Muñoz, Marcos David; Chatterjee, Saurabh; et al.; Inmuno-spin trapping from biochemistry to medicine: advances, challenges, and pitfalls. Focus on protein-centered radicals; Elsevier Science; Biochimica et Biophysica Acta- General Subjects; 1840; 2; 2-2014; 722-7290304-4165enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0304416513001943info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbagen.2013.04.039info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4078024/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:45:44Zoai:ri.conicet.gov.ar:11336/14656instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:45:44.248CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Inmuno-spin trapping from biochemistry to medicine: advances, challenges, and pitfalls. Focus on protein-centered radicals
title Inmuno-spin trapping from biochemistry to medicine: advances, challenges, and pitfalls. Focus on protein-centered radicals
spellingShingle Inmuno-spin trapping from biochemistry to medicine: advances, challenges, and pitfalls. Focus on protein-centered radicals
Gomez-Mejiba, Sandra Esther
ANTI-DMPO
DISEASE/EXPOSURE MODEL
IMMUNO-SPIN TRAPPING
PROTEIN RADICAL
REACTIVE CHEMICAL SPECIES
SPIN TRAP
title_short Inmuno-spin trapping from biochemistry to medicine: advances, challenges, and pitfalls. Focus on protein-centered radicals
title_full Inmuno-spin trapping from biochemistry to medicine: advances, challenges, and pitfalls. Focus on protein-centered radicals
title_fullStr Inmuno-spin trapping from biochemistry to medicine: advances, challenges, and pitfalls. Focus on protein-centered radicals
title_full_unstemmed Inmuno-spin trapping from biochemistry to medicine: advances, challenges, and pitfalls. Focus on protein-centered radicals
title_sort Inmuno-spin trapping from biochemistry to medicine: advances, challenges, and pitfalls. Focus on protein-centered radicals
dc.creator.none.fl_str_mv Gomez-Mejiba, Sandra Esther
Zhai, Zili
Della Vedova, Maria Cecilia
Muñoz, Marcos David
Chatterjee, Saurabh
Towner, Rheal A.
Hensley, Kenneth
Floyd, Robert A.
Mason, Ronald P.
Ramirez, Dario
author Gomez-Mejiba, Sandra Esther
author_facet Gomez-Mejiba, Sandra Esther
Zhai, Zili
Della Vedova, Maria Cecilia
Muñoz, Marcos David
Chatterjee, Saurabh
Towner, Rheal A.
Hensley, Kenneth
Floyd, Robert A.
Mason, Ronald P.
Ramirez, Dario
author_role author
author2 Zhai, Zili
Della Vedova, Maria Cecilia
Muñoz, Marcos David
Chatterjee, Saurabh
Towner, Rheal A.
Hensley, Kenneth
Floyd, Robert A.
Mason, Ronald P.
Ramirez, Dario
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTI-DMPO
DISEASE/EXPOSURE MODEL
IMMUNO-SPIN TRAPPING
PROTEIN RADICAL
REACTIVE CHEMICAL SPECIES
SPIN TRAP
topic ANTI-DMPO
DISEASE/EXPOSURE MODEL
IMMUNO-SPIN TRAPPING
PROTEIN RADICAL
REACTIVE CHEMICAL SPECIES
SPIN TRAP
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: Immuno-spin trapping (IST) is based on the reaction of a spin trap with a free radical to form a stable nitrone adduct, followed by the use of antibodies, rather than traditional electron paramagnetic resonance spectroscopy, to detect the nitrone adduct. IST has been successfully applied to mechanistic in vitro studies, and recently, macromolecule-centered radicals have been detected in models of drug-induced agranulocytosis, hepatotoxicity, cardiotoxicity, and ischemia/reperfusion, as well as in models of neurological, metabolic and immunological diseases. Scope of the review: To critically evaluate advances, challenges, and pitfalls as well as the scientific opportunities of IST as applied to the study of protein-centered free radicals generated in stressed organelles, cells, tissues and animal models of disease and exposure. Major conclusions: Because the spin trap has to be present at high enough concentrations in the microenvironment where the radical is formed, the possible effects of the spin trap on gene expression, metabolism and cell physiology have to be considered in the use of IST and in the interpretation of results. These factors have not yet been thoroughly dealt with in the literature. General significance: The identification of radicalized proteins during cell/tissue response to stressors will help define their role in the complex cellular response to stressors and pathogenesis; however, the fidelity of spin trapping/immuno-detection and the effects of the spin trap on the biological system should be considered.
Fil: Gomez-Mejiba, Sandra Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; Argentina
Fil: Zhai, Zili. University Of Chicago; Estados Unidos
Fil: Della Vedova, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; Argentina
Fil: Muñoz, Marcos David. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; Argentina
Fil: Chatterjee, Saurabh. University of South Carolina; Estados Unidos
Fil: Towner, Rheal A.. Oklahoma Medical Research Foundation; Estados Unidos
Fil: Hensley, Kenneth. University of Toledo; Estados Unidos
Fil: Floyd, Robert A.. Oklahoma Medical Research Foundation; Estados Unidos
Fil: Mason, Ronald P.. National Institute of Environmental Health Sciences; Estados Unidos
Fil: Ramirez, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Area de Biología Molecular; Argentina
description Background: Immuno-spin trapping (IST) is based on the reaction of a spin trap with a free radical to form a stable nitrone adduct, followed by the use of antibodies, rather than traditional electron paramagnetic resonance spectroscopy, to detect the nitrone adduct. IST has been successfully applied to mechanistic in vitro studies, and recently, macromolecule-centered radicals have been detected in models of drug-induced agranulocytosis, hepatotoxicity, cardiotoxicity, and ischemia/reperfusion, as well as in models of neurological, metabolic and immunological diseases. Scope of the review: To critically evaluate advances, challenges, and pitfalls as well as the scientific opportunities of IST as applied to the study of protein-centered free radicals generated in stressed organelles, cells, tissues and animal models of disease and exposure. Major conclusions: Because the spin trap has to be present at high enough concentrations in the microenvironment where the radical is formed, the possible effects of the spin trap on gene expression, metabolism and cell physiology have to be considered in the use of IST and in the interpretation of results. These factors have not yet been thoroughly dealt with in the literature. General significance: The identification of radicalized proteins during cell/tissue response to stressors will help define their role in the complex cellular response to stressors and pathogenesis; however, the fidelity of spin trapping/immuno-detection and the effects of the spin trap on the biological system should be considered.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14656
Gomez-Mejiba, Sandra Esther; Zhai, Zili; Della Vedova, Maria Cecilia; Muñoz, Marcos David; Chatterjee, Saurabh; et al.; Inmuno-spin trapping from biochemistry to medicine: advances, challenges, and pitfalls. Focus on protein-centered radicals; Elsevier Science; Biochimica et Biophysica Acta- General Subjects; 1840; 2; 2-2014; 722-729
0304-4165
url http://hdl.handle.net/11336/14656
identifier_str_mv Gomez-Mejiba, Sandra Esther; Zhai, Zili; Della Vedova, Maria Cecilia; Muñoz, Marcos David; Chatterjee, Saurabh; et al.; Inmuno-spin trapping from biochemistry to medicine: advances, challenges, and pitfalls. Focus on protein-centered radicals; Elsevier Science; Biochimica et Biophysica Acta- General Subjects; 1840; 2; 2-2014; 722-729
0304-4165
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0304416513001943
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbagen.2013.04.039
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4078024/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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