In vivo detection of free radicals in mouse septic encephalopathy using molecular MRI and immuno-spin trapping

Autores
Towner, Rheal A.; Garteiser, Philippe; Bozza, Fernando; Smith, Nataliya; Saunders, Debra; D'Avila, Joana C.P.; Magno, Flora; Oliveira, Marcus F.; Ehrenshaft, Marilyn; Lupu, Florea; Silasi Mansat, Robert; Ramirez, Dario; Gomez-Mejiba, Sandra Esther; Mason, Ronald P.; Castro Faria Neto, Hugo C.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Free radicals are known to play a major role in sepsis. Combined immuno-spin trapping and molecular magnetic resonance imaging (MRI) was used to detect in vivo and in situ levels of free radicals in murine septic encephalopathy after cecal ligation and puncture (CLP). DMPO (5,5-dimethyl pyrroline N-oxide) was injected over 6 h after CLP, before administration of an anti-DMPO probe (anti-DMPO antibody bound to albumin-gadolinium-diethylene triamine pentaacetic acid-biotin MRI targeting contrast agent). In vitro assessment of the anti-DMPO probe in oxidatively stressed mouse astrocytes significantly decreased T1 relaxation (p < 0.0001) compared to controls. MRI detected the presence of anti-DMPO adducts via a substantial decrease in %T1 change within the hippocampus, striatum, occipital, and medial cortex brain regions (p < 0.01 for all) in septic animals compared to shams, which was sustained for over 60 min (p < 0.05 for all). Fluorescently labeled streptavidin was used to target the anti-DMPO probe biotin, which was elevated in septic brain, liver, and lungs compared to sham. Ex vivo DMPO adducts (qualitative) and oxidative products, including 4-hydroxynonenal and 3-nitrotyrosine (quantitative, p < 0.05 for both), were elevated in septic brains compared to shams. This is the first study that has reported on the detection of in vivo and in situ levels of free radicals in murine septic encephalopathy. © 2013 Elsevier Inc.
Fil: Towner, Rheal A.. Oklahoma Medical Research Foundation; Estados Unidos
Fil: Garteiser, Philippe. Oklahoma Medical Research Foundation; Estados Unidos
Fil: Bozza, Fernando. Universidad de Porto; Portugal. Instituto Oswaldo Cruz; Brasil
Fil: Smith, Nataliya. Oklahoma Medical Research Foundation; Estados Unidos
Fil: Saunders, Debra. Oklahoma Medical Research Foundation; Estados Unidos. University of Utah; Estados Unidos
Fil: D'Avila, Joana C.P.. Governo Do Estado de Sao Paulo; Brasil. Instituto Oswaldo Cruz; Brasil
Fil: Magno, Flora. Instituto Oswaldo Cruz; Brasil
Fil: Oliveira, Marcus F.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Ehrenshaft, Marilyn. National Institutes Of Health. Departament Of Healt & Human; Estados Unidos
Fil: Lupu, Florea. Oklahoma Medical Research Foundation; Estados Unidos
Fil: Silasi Mansat, Robert. Oklahoma Medical Research Foundation; Estados Unidos
Fil: Ramirez, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Gomez-Mejiba, Sandra Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Mason, Ronald P.. National Institutes Of Health. Departament Of Healt & Human; Estados Unidos
Fil: Castro Faria Neto, Hugo C.. Instituto Oswaldo Cruz; Brasil
Materia
3-NITROTYROSINE
4-HYDROXYNONENAL
CECAL LIGATION AND PUNCTURE
DMPO
FLUORESCENCE MICROSCOPY
FREE RADICALS
IMMUNO-SPIN TRAPPING
MICE IN VIVO
MOLECULAR MRI
SEPSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/183475
Acceder
id CONICETDig_a4597a30763fff5ea61cf42a8c37d3e7
oai_identifier_str oai:ri.conicet.gov.ar:11336/183475
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vivo detection of free radicals in mouse septic encephalopathy using molecular MRI and immuno-spin trappingTowner, Rheal A.Garteiser, PhilippeBozza, FernandoSmith, NataliyaSaunders, DebraD'Avila, Joana C.P.Magno, FloraOliveira, Marcus F.Ehrenshaft, MarilynLupu, FloreaSilasi Mansat, RobertRamirez, DarioGomez-Mejiba, Sandra EstherMason, Ronald P.Castro Faria Neto, Hugo C.3-NITROTYROSINE4-HYDROXYNONENALCECAL LIGATION AND PUNCTUREDMPOFLUORESCENCE MICROSCOPYFREE RADICALSIMMUNO-SPIN TRAPPINGMICE IN VIVOMOLECULAR MRISEPSIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Free radicals are known to play a major role in sepsis. Combined immuno-spin trapping and molecular magnetic resonance imaging (MRI) was used to detect in vivo and in situ levels of free radicals in murine septic encephalopathy after cecal ligation and puncture (CLP). DMPO (5,5-dimethyl pyrroline N-oxide) was injected over 6 h after CLP, before administration of an anti-DMPO probe (anti-DMPO antibody bound to albumin-gadolinium-diethylene triamine pentaacetic acid-biotin MRI targeting contrast agent). In vitro assessment of the anti-DMPO probe in oxidatively stressed mouse astrocytes significantly decreased T1 relaxation (p < 0.0001) compared to controls. MRI detected the presence of anti-DMPO adducts via a substantial decrease in %T1 change within the hippocampus, striatum, occipital, and medial cortex brain regions (p < 0.01 for all) in septic animals compared to shams, which was sustained for over 60 min (p < 0.05 for all). Fluorescently labeled streptavidin was used to target the anti-DMPO probe biotin, which was elevated in septic brain, liver, and lungs compared to sham. Ex vivo DMPO adducts (qualitative) and oxidative products, including 4-hydroxynonenal and 3-nitrotyrosine (quantitative, p < 0.05 for both), were elevated in septic brains compared to shams. This is the first study that has reported on the detection of in vivo and in situ levels of free radicals in murine septic encephalopathy. © 2013 Elsevier Inc.Fil: Towner, Rheal A.. Oklahoma Medical Research Foundation; Estados UnidosFil: Garteiser, Philippe. Oklahoma Medical Research Foundation; Estados UnidosFil: Bozza, Fernando. Universidad de Porto; Portugal. Instituto Oswaldo Cruz; BrasilFil: Smith, Nataliya. Oklahoma Medical Research Foundation; Estados UnidosFil: Saunders, Debra. Oklahoma Medical Research Foundation; Estados Unidos. University of Utah; Estados UnidosFil: D'Avila, Joana C.P.. Governo Do Estado de Sao Paulo; Brasil. Instituto Oswaldo Cruz; BrasilFil: Magno, Flora. Instituto Oswaldo Cruz; BrasilFil: Oliveira, Marcus F.. Universidade Federal do Rio de Janeiro; BrasilFil: Ehrenshaft, Marilyn. National Institutes Of Health. Departament Of Healt & Human; Estados UnidosFil: Lupu, Florea. Oklahoma Medical Research Foundation; Estados UnidosFil: Silasi Mansat, Robert. Oklahoma Medical Research Foundation; Estados UnidosFil: Ramirez, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Gomez-Mejiba, Sandra Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Mason, Ronald P.. National Institutes Of Health. Departament Of Healt & Human; Estados UnidosFil: Castro Faria Neto, Hugo C.. Instituto Oswaldo Cruz; BrasilElsevier Science Inc.2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/183475Towner, Rheal A.; Garteiser, Philippe; Bozza, Fernando; Smith, Nataliya; Saunders, Debra; et al.; In vivo detection of free radicals in mouse septic encephalopathy using molecular MRI and immuno-spin trapping; Elsevier Science Inc.; Free Radical Biology and Medicine; 65; 2-2013; 828-8370891-58491873-4596CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0891584913005753info:eu-repo/semantics/altIdentifier/doi/10.1016/j.freeradbiomed.2013.08.172info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:32:31Zoai:ri.conicet.gov.ar:11336/183475instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:32:32.139CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vivo detection of free radicals in mouse septic encephalopathy using molecular MRI and immuno-spin trapping
title In vivo detection of free radicals in mouse septic encephalopathy using molecular MRI and immuno-spin trapping
spellingShingle In vivo detection of free radicals in mouse septic encephalopathy using molecular MRI and immuno-spin trapping
Towner, Rheal A.
3-NITROTYROSINE
4-HYDROXYNONENAL
CECAL LIGATION AND PUNCTURE
DMPO
FLUORESCENCE MICROSCOPY
FREE RADICALS
IMMUNO-SPIN TRAPPING
MICE IN VIVO
MOLECULAR MRI
SEPSIS
title_short In vivo detection of free radicals in mouse septic encephalopathy using molecular MRI and immuno-spin trapping
title_full In vivo detection of free radicals in mouse septic encephalopathy using molecular MRI and immuno-spin trapping
title_fullStr In vivo detection of free radicals in mouse septic encephalopathy using molecular MRI and immuno-spin trapping
title_full_unstemmed In vivo detection of free radicals in mouse septic encephalopathy using molecular MRI and immuno-spin trapping
title_sort In vivo detection of free radicals in mouse septic encephalopathy using molecular MRI and immuno-spin trapping
dc.creator.none.fl_str_mv Towner, Rheal A.
Garteiser, Philippe
Bozza, Fernando
Smith, Nataliya
Saunders, Debra
D'Avila, Joana C.P.
Magno, Flora
Oliveira, Marcus F.
Ehrenshaft, Marilyn
Lupu, Florea
Silasi Mansat, Robert
Ramirez, Dario
Gomez-Mejiba, Sandra Esther
Mason, Ronald P.
Castro Faria Neto, Hugo C.
author Towner, Rheal A.
author_facet Towner, Rheal A.
Garteiser, Philippe
Bozza, Fernando
Smith, Nataliya
Saunders, Debra
D'Avila, Joana C.P.
Magno, Flora
Oliveira, Marcus F.
Ehrenshaft, Marilyn
Lupu, Florea
Silasi Mansat, Robert
Ramirez, Dario
Gomez-Mejiba, Sandra Esther
Mason, Ronald P.
Castro Faria Neto, Hugo C.
author_role author
author2 Garteiser, Philippe
Bozza, Fernando
Smith, Nataliya
Saunders, Debra
D'Avila, Joana C.P.
Magno, Flora
Oliveira, Marcus F.
Ehrenshaft, Marilyn
Lupu, Florea
Silasi Mansat, Robert
Ramirez, Dario
Gomez-Mejiba, Sandra Esther
Mason, Ronald P.
Castro Faria Neto, Hugo C.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv 3-NITROTYROSINE
4-HYDROXYNONENAL
CECAL LIGATION AND PUNCTURE
DMPO
FLUORESCENCE MICROSCOPY
FREE RADICALS
IMMUNO-SPIN TRAPPING
MICE IN VIVO
MOLECULAR MRI
SEPSIS
topic 3-NITROTYROSINE
4-HYDROXYNONENAL
CECAL LIGATION AND PUNCTURE
DMPO
FLUORESCENCE MICROSCOPY
FREE RADICALS
IMMUNO-SPIN TRAPPING
MICE IN VIVO
MOLECULAR MRI
SEPSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Free radicals are known to play a major role in sepsis. Combined immuno-spin trapping and molecular magnetic resonance imaging (MRI) was used to detect in vivo and in situ levels of free radicals in murine septic encephalopathy after cecal ligation and puncture (CLP). DMPO (5,5-dimethyl pyrroline N-oxide) was injected over 6 h after CLP, before administration of an anti-DMPO probe (anti-DMPO antibody bound to albumin-gadolinium-diethylene triamine pentaacetic acid-biotin MRI targeting contrast agent). In vitro assessment of the anti-DMPO probe in oxidatively stressed mouse astrocytes significantly decreased T1 relaxation (p < 0.0001) compared to controls. MRI detected the presence of anti-DMPO adducts via a substantial decrease in %T1 change within the hippocampus, striatum, occipital, and medial cortex brain regions (p < 0.01 for all) in septic animals compared to shams, which was sustained for over 60 min (p < 0.05 for all). Fluorescently labeled streptavidin was used to target the anti-DMPO probe biotin, which was elevated in septic brain, liver, and lungs compared to sham. Ex vivo DMPO adducts (qualitative) and oxidative products, including 4-hydroxynonenal and 3-nitrotyrosine (quantitative, p < 0.05 for both), were elevated in septic brains compared to shams. This is the first study that has reported on the detection of in vivo and in situ levels of free radicals in murine septic encephalopathy. © 2013 Elsevier Inc.
Fil: Towner, Rheal A.. Oklahoma Medical Research Foundation; Estados Unidos
Fil: Garteiser, Philippe. Oklahoma Medical Research Foundation; Estados Unidos
Fil: Bozza, Fernando. Universidad de Porto; Portugal. Instituto Oswaldo Cruz; Brasil
Fil: Smith, Nataliya. Oklahoma Medical Research Foundation; Estados Unidos
Fil: Saunders, Debra. Oklahoma Medical Research Foundation; Estados Unidos. University of Utah; Estados Unidos
Fil: D'Avila, Joana C.P.. Governo Do Estado de Sao Paulo; Brasil. Instituto Oswaldo Cruz; Brasil
Fil: Magno, Flora. Instituto Oswaldo Cruz; Brasil
Fil: Oliveira, Marcus F.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Ehrenshaft, Marilyn. National Institutes Of Health. Departament Of Healt & Human; Estados Unidos
Fil: Lupu, Florea. Oklahoma Medical Research Foundation; Estados Unidos
Fil: Silasi Mansat, Robert. Oklahoma Medical Research Foundation; Estados Unidos
Fil: Ramirez, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Gomez-Mejiba, Sandra Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Mason, Ronald P.. National Institutes Of Health. Departament Of Healt & Human; Estados Unidos
Fil: Castro Faria Neto, Hugo C.. Instituto Oswaldo Cruz; Brasil
description Free radicals are known to play a major role in sepsis. Combined immuno-spin trapping and molecular magnetic resonance imaging (MRI) was used to detect in vivo and in situ levels of free radicals in murine septic encephalopathy after cecal ligation and puncture (CLP). DMPO (5,5-dimethyl pyrroline N-oxide) was injected over 6 h after CLP, before administration of an anti-DMPO probe (anti-DMPO antibody bound to albumin-gadolinium-diethylene triamine pentaacetic acid-biotin MRI targeting contrast agent). In vitro assessment of the anti-DMPO probe in oxidatively stressed mouse astrocytes significantly decreased T1 relaxation (p < 0.0001) compared to controls. MRI detected the presence of anti-DMPO adducts via a substantial decrease in %T1 change within the hippocampus, striatum, occipital, and medial cortex brain regions (p < 0.01 for all) in septic animals compared to shams, which was sustained for over 60 min (p < 0.05 for all). Fluorescently labeled streptavidin was used to target the anti-DMPO probe biotin, which was elevated in septic brain, liver, and lungs compared to sham. Ex vivo DMPO adducts (qualitative) and oxidative products, including 4-hydroxynonenal and 3-nitrotyrosine (quantitative, p < 0.05 for both), were elevated in septic brains compared to shams. This is the first study that has reported on the detection of in vivo and in situ levels of free radicals in murine septic encephalopathy. © 2013 Elsevier Inc.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/183475
Towner, Rheal A.; Garteiser, Philippe; Bozza, Fernando; Smith, Nataliya; Saunders, Debra; et al.; In vivo detection of free radicals in mouse septic encephalopathy using molecular MRI and immuno-spin trapping; Elsevier Science Inc.; Free Radical Biology and Medicine; 65; 2-2013; 828-837
0891-5849
1873-4596
CONICET Digital
CONICET
url http://hdl.handle.net/11336/183475
identifier_str_mv Towner, Rheal A.; Garteiser, Philippe; Bozza, Fernando; Smith, Nataliya; Saunders, Debra; et al.; In vivo detection of free radicals in mouse septic encephalopathy using molecular MRI and immuno-spin trapping; Elsevier Science Inc.; Free Radical Biology and Medicine; 65; 2-2013; 828-837
0891-5849
1873-4596
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0891584913005753
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.freeradbiomed.2013.08.172
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc.
publisher.none.fl_str_mv Elsevier Science Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083457945436160
score 13.22299

Publicaciones similares