A chromosome‐level genome assembly enables the identification of the follicule stimulating hormone receptor as the master sex‐determining gene in the flatfish Solea senegalensis...

Autores: de la Herrán, Roberto; Hermida, Miguel; Rubiolo, Juan Andrés; Gómez Garrido, Jéssica; Cruz, Fernando; Robles, Francisca; Navajas Pérez, Rafael; Blanco, Andres; Villamayor, Paula Rodriguez; Torres, Dorinda; Sánchez Quinteiro, Pablo; Ramirez, Daniel; Rodríguez, Maria Esther; Arias Pérez, Alberto; Cross, Ismael; Duncan, Neil; Martínez Peña, Teresa; Riaza, Ana; Millán, Adrian; De Rosa, M. Cristina; Pirolli, Davide; Gut, Marta; Bouza, Carmen; Robledo, Diego; Rebordinos, Laureana; Alioto, Tyler; Ruíz Rejón, Carmelo; Martínez, Paulino
Año de publicación: 2023
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Sex determination (SD) shows huge variation among fish and a high evolutionary rate, as illustrated by the Pleuronectiformes (flatfishes). This order is characterized by its adaptation to demersal life, compact genomes and diversity of SD mechanisms. Here, we assembled the Solea senegalensis genome, a flatfish of great commercial value, into 82 contigs (614 Mb) combining long- and short-read sequencing, which were next scaffolded using a highly dense genetic map (28,838 markers, 21 linkage groups), representing 98.9% of the assembly. Further, we established the correspondence between the assembly and the 21 chromosomes by using BAC-FISH. Whole genome resequencing of six males and six females enabled the identification of 41 single nucleotide polymorphism variants in the follicle stimulating hormone receptor (fshr) consistent with an XX/XY SD system. The observed sex association was validated in a broader independent sample, providing a novel molecular sexing tool. The fshr gene displayed differential expression between male and female gonads from 86 days post-fertilization, when the gonad is still an undifferentiated primordium, concomitant with the activation of amh and cyp19a1a, testis and ovary marker genes, respectively, in males and females. The Y-linked fshr allele, which included 24 nonsynonymous variants and showed a highly divergent 3D protein structure, was overexpressed in males compared to the X-linked allele at all stages of gonadal differentiation. We hypothesize a mechanism hampering the action of the follicle stimulating hormone driving the undifferentiated gonad toward testis.
Fil: de la Herrán, Roberto. Universidad de Granada. Facultad de Ciencias; España
Fil: Hermida, Miguel. Universidad de Santiago de Compostela; España
Fil: Rubiolo, Juan Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Santiago de Compostela; España
Fil: Gómez Garrido, Jéssica. Centre Nacional D'anàlisi Genòmica (cnag-crg); España
Fil: Cruz, Fernando. Centre de Regulació Genómica. Centre Nacional D'anàlisi Genòmica; España
Fil: Robles, Francisca. Universidad de Granada. Facultad de Ciencias; España
Fil: Navajas Pérez, Rafael. Universidad de Granada. Facultad de Ciencias; España
Fil: Blanco, Andres. Universidad de Santiago de Compostela; España
Fil: Villamayor, Paula Rodriguez. Universidad de Santiago de Compostela; España
Fil: Torres, Dorinda. Universidad de Santiago de Compostela; España
Fil: Sánchez Quinteiro, Pablo. Universidad de Santiago de Compostela; España
Fil: Ramirez, Daniel. Universidad de Cádiz; España
Fil: Rodríguez, Maria Esther. Universidad de Cádiz; España
Fil: Arias Pérez, Alberto. Universidad de Cádiz; España
Fil: Cross, Ismael. Universidad de Cádiz; España
Fil: Duncan, Neil. Instituto de Investigación y Tecnología Agroalimentarias; España
Fil: Martínez Peña, Teresa. Stolt Sea Farm SA; España
Fil: Riaza, Ana. Stolt Sea Farm Sa, Departamento I+d; España
Fil: Millán, Adrian. Geneaqua SL; España
Fil: De Rosa, M. Cristina. Consiglio Nazionale delle Ricerche. Istituto di Scienze e Tecnologie Chimiche "Giulio Natta" ; Italia
Fil: Pirolli, Davide. Consiglio Nazionale delle Ricerche. Istituto di Scienze e Tecnologie Chimiche "Giulio Natta" ; Italia
Fil: Gut, Marta. Centre de Regulació Genómica. Centre Nacional D'anàlisi Genòmica; España
Fil: Bouza, Carmen. Universidad de Santiago de Compostela; España
Fil: Robledo, Diego. University of Edinburgh; Reino Unido
Fil: Rebordinos, Laureana. Universidad de Cádiz; España
Fil: Alioto, Tyler. Centre de Regulació Genómica. Centre Nacional D'anàlisi Genòmica; España. Universitat Pompeu Fabra; España
Fil: Ruíz Rejón, Carmelo. Universidad de Granada. Facultad de Ciencias; España
Fil: Martínez, Paulino. Universidad de Santiago de Compostela; España
Materia: Sex determination
Pleuronectiformes
Solea senegalensis
Genome assembly
Flatfish
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/274462

Acceder

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oai_identifier_str	oai:ri.conicet.gov.ar:11336/274462
network_acronym_str	CONICETDig
repository_id_str	3498
network_name_str	CONICET Digital (CONICET)
spelling	A chromosome‐level genome assembly enables the identification of the follicule stimulating hormone receptor as the master sex‐determining gene in the flatfish Solea senegalensisde la Herrán, RobertoHermida, MiguelRubiolo, Juan AndrésGómez Garrido, JéssicaCruz, FernandoRobles, FranciscaNavajas Pérez, RafaelBlanco, AndresVillamayor, Paula RodriguezTorres, DorindaSánchez Quinteiro, PabloRamirez, DanielRodríguez, Maria EstherArias Pérez, AlbertoCross, IsmaelDuncan, NeilMartínez Peña, TeresaRiaza, AnaMillán, AdrianDe Rosa, M. CristinaPirolli, DavideGut, MartaBouza, CarmenRobledo, DiegoRebordinos, LaureanaAlioto, TylerRuíz Rejón, CarmeloMartínez, PaulinoSex determinationPleuronectiformesSolea senegalensisGenome assemblyFlatfishhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Sex determination (SD) shows huge variation among fish and a high evolutionary rate, as illustrated by the Pleuronectiformes (flatfishes). This order is characterized by its adaptation to demersal life, compact genomes and diversity of SD mechanisms. Here, we assembled the Solea senegalensis genome, a flatfish of great commercial value, into 82 contigs (614 Mb) combining long- and short-read sequencing, which were next scaffolded using a highly dense genetic map (28,838 markers, 21 linkage groups), representing 98.9% of the assembly. Further, we established the correspondence between the assembly and the 21 chromosomes by using BAC-FISH. Whole genome resequencing of six males and six females enabled the identification of 41 single nucleotide polymorphism variants in the follicle stimulating hormone receptor (fshr) consistent with an XX/XY SD system. The observed sex association was validated in a broader independent sample, providing a novel molecular sexing tool. The fshr gene displayed differential expression between male and female gonads from 86 days post-fertilization, when the gonad is still an undifferentiated primordium, concomitant with the activation of amh and cyp19a1a, testis and ovary marker genes, respectively, in males and females. The Y-linked fshr allele, which included 24 nonsynonymous variants and showed a highly divergent 3D protein structure, was overexpressed in males compared to the X-linked allele at all stages of gonadal differentiation. We hypothesize a mechanism hampering the action of the follicle stimulating hormone driving the undifferentiated gonad toward testis.Fil: de la Herrán, Roberto. Universidad de Granada. Facultad de Ciencias; EspañaFil: Hermida, Miguel. Universidad de Santiago de Compostela; EspañaFil: Rubiolo, Juan Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Santiago de Compostela; EspañaFil: Gómez Garrido, Jéssica. Centre Nacional D'anàlisi Genòmica (cnag-crg); EspañaFil: Cruz, Fernando. Centre de Regulació Genómica. Centre Nacional D'anàlisi Genòmica; EspañaFil: Robles, Francisca. Universidad de Granada. Facultad de Ciencias; EspañaFil: Navajas Pérez, Rafael. Universidad de Granada. Facultad de Ciencias; EspañaFil: Blanco, Andres. Universidad de Santiago de Compostela; EspañaFil: Villamayor, Paula Rodriguez. Universidad de Santiago de Compostela; EspañaFil: Torres, Dorinda. Universidad de Santiago de Compostela; EspañaFil: Sánchez Quinteiro, Pablo. Universidad de Santiago de Compostela; EspañaFil: Ramirez, Daniel. Universidad de Cádiz; EspañaFil: Rodríguez, Maria Esther. Universidad de Cádiz; EspañaFil: Arias Pérez, Alberto. Universidad de Cádiz; EspañaFil: Cross, Ismael. Universidad de Cádiz; EspañaFil: Duncan, Neil. Instituto de Investigación y Tecnología Agroalimentarias; EspañaFil: Martínez Peña, Teresa. Stolt Sea Farm SA; EspañaFil: Riaza, Ana. Stolt Sea Farm Sa, Departamento I+d; EspañaFil: Millán, Adrian. Geneaqua SL; EspañaFil: De Rosa, M. Cristina. Consiglio Nazionale delle Ricerche. Istituto di Scienze e Tecnologie Chimiche "Giulio Natta" ; ItaliaFil: Pirolli, Davide. Consiglio Nazionale delle Ricerche. Istituto di Scienze e Tecnologie Chimiche "Giulio Natta" ; ItaliaFil: Gut, Marta. Centre de Regulació Genómica. Centre Nacional D'anàlisi Genòmica; EspañaFil: Bouza, Carmen. Universidad de Santiago de Compostela; EspañaFil: Robledo, Diego. University of Edinburgh; Reino UnidoFil: Rebordinos, Laureana. Universidad de Cádiz; EspañaFil: Alioto, Tyler. Centre de Regulació Genómica. Centre Nacional D'anàlisi Genòmica; España. Universitat Pompeu Fabra; EspañaFil: Ruíz Rejón, Carmelo. Universidad de Granada. Facultad de Ciencias; EspañaFil: Martínez, Paulino. Universidad de Santiago de Compostela; EspañaWiley Blackwell Publishing, Inc2023-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/274462de la Herrán, Roberto; Hermida, Miguel; Rubiolo, Juan Andrés; Gómez Garrido, Jéssica; Cruz, Fernando; et al.; A chromosome‐level genome assembly enables the identification of the follicule stimulating hormone receptor as the master sex‐determining gene in the flatfish Solea senegalensis; Wiley Blackwell Publishing, Inc; Molecular Ecology Resources; 23; 4; 1-2023; 886-9041755-098XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/1755-0998.13750info:eu-repo/semantics/altIdentifier/doi/10.1111/1755-0998.13750info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-26T08:54:46Zoai:ri.conicet.gov.ar:11336/274462instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-26 08:54:46.63CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	A chromosome‐level genome assembly enables the identification of the follicule stimulating hormone receptor as the master sex‐determining gene in the flatfish Solea senegalensis
title	A chromosome‐level genome assembly enables the identification of the follicule stimulating hormone receptor as the master sex‐determining gene in the flatfish Solea senegalensis
spellingShingle	A chromosome‐level genome assembly enables the identification of the follicule stimulating hormone receptor as the master sex‐determining gene in the flatfish Solea senegalensis de la Herrán, Roberto Sex determination Pleuronectiformes Solea senegalensis Genome assembly Flatfish
title_short	A chromosome‐level genome assembly enables the identification of the follicule stimulating hormone receptor as the master sex‐determining gene in the flatfish Solea senegalensis
title_full	A chromosome‐level genome assembly enables the identification of the follicule stimulating hormone receptor as the master sex‐determining gene in the flatfish Solea senegalensis
title_fullStr	A chromosome‐level genome assembly enables the identification of the follicule stimulating hormone receptor as the master sex‐determining gene in the flatfish Solea senegalensis
title_full_unstemmed	A chromosome‐level genome assembly enables the identification of the follicule stimulating hormone receptor as the master sex‐determining gene in the flatfish Solea senegalensis
title_sort	A chromosome‐level genome assembly enables the identification of the follicule stimulating hormone receptor as the master sex‐determining gene in the flatfish Solea senegalensis
dc.creator.none.fl_str_mv	de la Herrán, Roberto Hermida, Miguel Rubiolo, Juan Andrés Gómez Garrido, Jéssica Cruz, Fernando Robles, Francisca Navajas Pérez, Rafael Blanco, Andres Villamayor, Paula Rodriguez Torres, Dorinda Sánchez Quinteiro, Pablo Ramirez, Daniel Rodríguez, Maria Esther Arias Pérez, Alberto Cross, Ismael Duncan, Neil Martínez Peña, Teresa Riaza, Ana Millán, Adrian De Rosa, M. Cristina Pirolli, Davide Gut, Marta Bouza, Carmen Robledo, Diego Rebordinos, Laureana Alioto, Tyler Ruíz Rejón, Carmelo Martínez, Paulino
author	de la Herrán, Roberto
author_facet	de la Herrán, Roberto Hermida, Miguel Rubiolo, Juan Andrés Gómez Garrido, Jéssica Cruz, Fernando Robles, Francisca Navajas Pérez, Rafael Blanco, Andres Villamayor, Paula Rodriguez Torres, Dorinda Sánchez Quinteiro, Pablo Ramirez, Daniel Rodríguez, Maria Esther Arias Pérez, Alberto Cross, Ismael Duncan, Neil Martínez Peña, Teresa Riaza, Ana Millán, Adrian De Rosa, M. Cristina Pirolli, Davide Gut, Marta Bouza, Carmen Robledo, Diego Rebordinos, Laureana Alioto, Tyler Ruíz Rejón, Carmelo Martínez, Paulino
author_role	author
author2	Hermida, Miguel Rubiolo, Juan Andrés Gómez Garrido, Jéssica Cruz, Fernando Robles, Francisca Navajas Pérez, Rafael Blanco, Andres Villamayor, Paula Rodriguez Torres, Dorinda Sánchez Quinteiro, Pablo Ramirez, Daniel Rodríguez, Maria Esther Arias Pérez, Alberto Cross, Ismael Duncan, Neil Martínez Peña, Teresa Riaza, Ana Millán, Adrian De Rosa, M. Cristina Pirolli, Davide Gut, Marta Bouza, Carmen Robledo, Diego Rebordinos, Laureana Alioto, Tyler Ruíz Rejón, Carmelo Martínez, Paulino
author2_role	author author author author author author author author author author author author author author author author author author author author author author author author author author author
dc.subject.none.fl_str_mv	Sex determination Pleuronectiformes Solea senegalensis Genome assembly Flatfish
topic	Sex determination Pleuronectiformes Solea senegalensis Genome assembly Flatfish
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv	Sex determination (SD) shows huge variation among fish and a high evolutionary rate, as illustrated by the Pleuronectiformes (flatfishes). This order is characterized by its adaptation to demersal life, compact genomes and diversity of SD mechanisms. Here, we assembled the Solea senegalensis genome, a flatfish of great commercial value, into 82 contigs (614 Mb) combining long- and short-read sequencing, which were next scaffolded using a highly dense genetic map (28,838 markers, 21 linkage groups), representing 98.9% of the assembly. Further, we established the correspondence between the assembly and the 21 chromosomes by using BAC-FISH. Whole genome resequencing of six males and six females enabled the identification of 41 single nucleotide polymorphism variants in the follicle stimulating hormone receptor (fshr) consistent with an XX/XY SD system. The observed sex association was validated in a broader independent sample, providing a novel molecular sexing tool. The fshr gene displayed differential expression between male and female gonads from 86 days post-fertilization, when the gonad is still an undifferentiated primordium, concomitant with the activation of amh and cyp19a1a, testis and ovary marker genes, respectively, in males and females. The Y-linked fshr allele, which included 24 nonsynonymous variants and showed a highly divergent 3D protein structure, was overexpressed in males compared to the X-linked allele at all stages of gonadal differentiation. We hypothesize a mechanism hampering the action of the follicle stimulating hormone driving the undifferentiated gonad toward testis. Fil: de la Herrán, Roberto. Universidad de Granada. Facultad de Ciencias; España Fil: Hermida, Miguel. Universidad de Santiago de Compostela; España Fil: Rubiolo, Juan Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Santiago de Compostela; España Fil: Gómez Garrido, Jéssica. Centre Nacional D'anàlisi Genòmica (cnag-crg); España Fil: Cruz, Fernando. Centre de Regulació Genómica. Centre Nacional D'anàlisi Genòmica; España Fil: Robles, Francisca. Universidad de Granada. Facultad de Ciencias; España Fil: Navajas Pérez, Rafael. Universidad de Granada. Facultad de Ciencias; España Fil: Blanco, Andres. Universidad de Santiago de Compostela; España Fil: Villamayor, Paula Rodriguez. Universidad de Santiago de Compostela; España Fil: Torres, Dorinda. Universidad de Santiago de Compostela; España Fil: Sánchez Quinteiro, Pablo. Universidad de Santiago de Compostela; España Fil: Ramirez, Daniel. Universidad de Cádiz; España Fil: Rodríguez, Maria Esther. Universidad de Cádiz; España Fil: Arias Pérez, Alberto. Universidad de Cádiz; España Fil: Cross, Ismael. Universidad de Cádiz; España Fil: Duncan, Neil. Instituto de Investigación y Tecnología Agroalimentarias; España Fil: Martínez Peña, Teresa. Stolt Sea Farm SA; España Fil: Riaza, Ana. Stolt Sea Farm Sa, Departamento I+d; España Fil: Millán, Adrian. Geneaqua SL; España Fil: De Rosa, M. Cristina. Consiglio Nazionale delle Ricerche. Istituto di Scienze e Tecnologie Chimiche "Giulio Natta" ; Italia Fil: Pirolli, Davide. Consiglio Nazionale delle Ricerche. Istituto di Scienze e Tecnologie Chimiche "Giulio Natta" ; Italia Fil: Gut, Marta. Centre de Regulació Genómica. Centre Nacional D'anàlisi Genòmica; España Fil: Bouza, Carmen. Universidad de Santiago de Compostela; España Fil: Robledo, Diego. University of Edinburgh; Reino Unido Fil: Rebordinos, Laureana. Universidad de Cádiz; España Fil: Alioto, Tyler. Centre de Regulació Genómica. Centre Nacional D'anàlisi Genòmica; España. Universitat Pompeu Fabra; España Fil: Ruíz Rejón, Carmelo. Universidad de Granada. Facultad de Ciencias; España Fil: Martínez, Paulino. Universidad de Santiago de Compostela; España
description	Sex determination (SD) shows huge variation among fish and a high evolutionary rate, as illustrated by the Pleuronectiformes (flatfishes). This order is characterized by its adaptation to demersal life, compact genomes and diversity of SD mechanisms. Here, we assembled the Solea senegalensis genome, a flatfish of great commercial value, into 82 contigs (614 Mb) combining long- and short-read sequencing, which were next scaffolded using a highly dense genetic map (28,838 markers, 21 linkage groups), representing 98.9% of the assembly. Further, we established the correspondence between the assembly and the 21 chromosomes by using BAC-FISH. Whole genome resequencing of six males and six females enabled the identification of 41 single nucleotide polymorphism variants in the follicle stimulating hormone receptor (fshr) consistent with an XX/XY SD system. The observed sex association was validated in a broader independent sample, providing a novel molecular sexing tool. The fshr gene displayed differential expression between male and female gonads from 86 days post-fertilization, when the gonad is still an undifferentiated primordium, concomitant with the activation of amh and cyp19a1a, testis and ovary marker genes, respectively, in males and females. The Y-linked fshr allele, which included 24 nonsynonymous variants and showed a highly divergent 3D protein structure, was overexpressed in males compared to the X-linked allele at all stages of gonadal differentiation. We hypothesize a mechanism hampering the action of the follicle stimulating hormone driving the undifferentiated gonad toward testis.
publishDate	2023
dc.date.none.fl_str_mv	2023-01
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/274462 de la Herrán, Roberto; Hermida, Miguel; Rubiolo, Juan Andrés; Gómez Garrido, Jéssica; Cruz, Fernando; et al.; A chromosome‐level genome assembly enables the identification of the follicule stimulating hormone receptor as the master sex‐determining gene in the flatfish Solea senegalensis; Wiley Blackwell Publishing, Inc; Molecular Ecology Resources; 23; 4; 1-2023; 886-904 1755-098X CONICET Digital CONICET
url	http://hdl.handle.net/11336/274462
identifier_str_mv	de la Herrán, Roberto; Hermida, Miguel; Rubiolo, Juan Andrés; Gómez Garrido, Jéssica; Cruz, Fernando; et al.; A chromosome‐level genome assembly enables the identification of the follicule stimulating hormone receptor as the master sex‐determining gene in the flatfish Solea senegalensis; Wiley Blackwell Publishing, Inc; Molecular Ecology Resources; 23; 4; 1-2023; 886-904 1755-098X CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/1755-0998.13750 info:eu-repo/semantics/altIdentifier/doi/10.1111/1755-0998.13750
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf
dc.publisher.none.fl_str_mv	Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv	Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
collection	CONICET Digital (CONICET)
instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score	13.011256

A chromosome‐level genome assembly enables the identification of the follicule stimulating hormone receptor as the master sex‐determining gene in the flatfish Solea senegalensis...

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