Analysis of truncated growth hormone receptor 1 in the differential growth of fine flounder Paralichthys adspersus

Autores
Marín, Alan; Alonso, Andrés Mariano; Delgadin, Tomás H.; López Landavery, Edgar A.; Cometivos, Lise Jara; Saavedra Flores, Anaid; Reyes Flores, Lorenzo E.; Yzásiga Barrera, Carmen G.; Fernandino, Juan Ignacio; Zelada Mázmela, Eliana
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Differences in body size within a fish population are a recurring problem in aquaculture. In fish farms, a higher growth rate is one of the most important selection traits, so growth dispersion with smaller batch sizes leads to increased production costs. Despite the importance of this dispersion in growth, the understanding of the molecular basis of these differences is still largely unknown. In the present work, we firstly determined that in the fine flounder Paralichthys adspersus the variant of the growth hormone receptor 1 (ghr1tr) is in agreement with a transcript truncation product of an alternative polyadenylation signal (APAs) embedded in an intronic region of ghr1 full length nucleotide sequence. Additionally, we observed that this region possesses a high identity with homologous regions in other Pleuronectiformes with documented differences in body size. Finally, our quantitative expression analysis, focusing on liver and muscle tissues, reveals that ghr1tr of P. adspersus was less expressed in bigger adult individuals. In this sense, this study proposes ghr1tr as the modulator of size dimorphism growth and that it could be a useful target for genetic studies on fine flounder breeding improvement.
Fil: Marín, Alan. Universidad Nacional del Santa; Perú
Fil: Alonso, Andrés Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Delgadin, Tomás H.. Universidad Nacional del Santa; Perú
Fil: López Landavery, Edgar A.. Universidad Nacional del Santa; Perú
Fil: Cometivos, Lise Jara. Universidad Nacional del Santa; Perú
Fil: Saavedra Flores, Anaid. Universidad Nacional del Santa; Perú
Fil: Reyes Flores, Lorenzo E.. Universidad Nacional del Santa; Perú
Fil: Yzásiga Barrera, Carmen G.. Universidad Nacional del Santa; Perú
Fil: Fernandino, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Zelada Mázmela, Eliana. Universidad Nacional del Santa; Perú
Materia
AQUACULTURE
FLATFISH
GHR1
GROWTH HORMONE
INTRONIC ALTERNATIVE POLYADENYLATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/221829
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/221829
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Analysis of truncated growth hormone receptor 1 in the differential growth of fine flounder Paralichthys adspersusMarín, AlanAlonso, Andrés MarianoDelgadin, Tomás H.López Landavery, Edgar A.Cometivos, Lise JaraSaavedra Flores, AnaidReyes Flores, Lorenzo E.Yzásiga Barrera, Carmen G.Fernandino, Juan IgnacioZelada Mázmela, ElianaAQUACULTUREFLATFISHGHR1GROWTH HORMONEINTRONIC ALTERNATIVE POLYADENYLATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Differences in body size within a fish population are a recurring problem in aquaculture. In fish farms, a higher growth rate is one of the most important selection traits, so growth dispersion with smaller batch sizes leads to increased production costs. Despite the importance of this dispersion in growth, the understanding of the molecular basis of these differences is still largely unknown. In the present work, we firstly determined that in the fine flounder Paralichthys adspersus the variant of the growth hormone receptor 1 (ghr1tr) is in agreement with a transcript truncation product of an alternative polyadenylation signal (APAs) embedded in an intronic region of ghr1 full length nucleotide sequence. Additionally, we observed that this region possesses a high identity with homologous regions in other Pleuronectiformes with documented differences in body size. Finally, our quantitative expression analysis, focusing on liver and muscle tissues, reveals that ghr1tr of P. adspersus was less expressed in bigger adult individuals. In this sense, this study proposes ghr1tr as the modulator of size dimorphism growth and that it could be a useful target for genetic studies on fine flounder breeding improvement.Fil: Marín, Alan. Universidad Nacional del Santa; PerúFil: Alonso, Andrés Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Delgadin, Tomás H.. Universidad Nacional del Santa; PerúFil: López Landavery, Edgar A.. Universidad Nacional del Santa; PerúFil: Cometivos, Lise Jara. Universidad Nacional del Santa; PerúFil: Saavedra Flores, Anaid. Universidad Nacional del Santa; PerúFil: Reyes Flores, Lorenzo E.. Universidad Nacional del Santa; PerúFil: Yzásiga Barrera, Carmen G.. Universidad Nacional del Santa; PerúFil: Fernandino, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Zelada Mázmela, Eliana. Universidad Nacional del Santa; PerúElsevier Science2023-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/221829Marín, Alan; Alonso, Andrés Mariano; Delgadin, Tomás H.; López Landavery, Edgar A.; Cometivos, Lise Jara; et al.; Analysis of truncated growth hormone receptor 1 in the differential growth of fine flounder Paralichthys adspersus; Elsevier Science; Aquaculture; 574; 9-2023; 1-80044-8486CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0044848623004659info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2023.739691info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:24:31Zoai:ri.conicet.gov.ar:11336/221829instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:24:31.544CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analysis of truncated growth hormone receptor 1 in the differential growth of fine flounder Paralichthys adspersus
title Analysis of truncated growth hormone receptor 1 in the differential growth of fine flounder Paralichthys adspersus
spellingShingle Analysis of truncated growth hormone receptor 1 in the differential growth of fine flounder Paralichthys adspersus
Marín, Alan
AQUACULTURE
FLATFISH
GHR1
GROWTH HORMONE
INTRONIC ALTERNATIVE POLYADENYLATION
title_short Analysis of truncated growth hormone receptor 1 in the differential growth of fine flounder Paralichthys adspersus
title_full Analysis of truncated growth hormone receptor 1 in the differential growth of fine flounder Paralichthys adspersus
title_fullStr Analysis of truncated growth hormone receptor 1 in the differential growth of fine flounder Paralichthys adspersus
title_full_unstemmed Analysis of truncated growth hormone receptor 1 in the differential growth of fine flounder Paralichthys adspersus
title_sort Analysis of truncated growth hormone receptor 1 in the differential growth of fine flounder Paralichthys adspersus
dc.creator.none.fl_str_mv Marín, Alan
Alonso, Andrés Mariano
Delgadin, Tomás H.
López Landavery, Edgar A.
Cometivos, Lise Jara
Saavedra Flores, Anaid
Reyes Flores, Lorenzo E.
Yzásiga Barrera, Carmen G.
Fernandino, Juan Ignacio
Zelada Mázmela, Eliana
author Marín, Alan
author_facet Marín, Alan
Alonso, Andrés Mariano
Delgadin, Tomás H.
López Landavery, Edgar A.
Cometivos, Lise Jara
Saavedra Flores, Anaid
Reyes Flores, Lorenzo E.
Yzásiga Barrera, Carmen G.
Fernandino, Juan Ignacio
Zelada Mázmela, Eliana
author_role author
author2 Alonso, Andrés Mariano
Delgadin, Tomás H.
López Landavery, Edgar A.
Cometivos, Lise Jara
Saavedra Flores, Anaid
Reyes Flores, Lorenzo E.
Yzásiga Barrera, Carmen G.
Fernandino, Juan Ignacio
Zelada Mázmela, Eliana
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AQUACULTURE
FLATFISH
GHR1
GROWTH HORMONE
INTRONIC ALTERNATIVE POLYADENYLATION
topic AQUACULTURE
FLATFISH
GHR1
GROWTH HORMONE
INTRONIC ALTERNATIVE POLYADENYLATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Differences in body size within a fish population are a recurring problem in aquaculture. In fish farms, a higher growth rate is one of the most important selection traits, so growth dispersion with smaller batch sizes leads to increased production costs. Despite the importance of this dispersion in growth, the understanding of the molecular basis of these differences is still largely unknown. In the present work, we firstly determined that in the fine flounder Paralichthys adspersus the variant of the growth hormone receptor 1 (ghr1tr) is in agreement with a transcript truncation product of an alternative polyadenylation signal (APAs) embedded in an intronic region of ghr1 full length nucleotide sequence. Additionally, we observed that this region possesses a high identity with homologous regions in other Pleuronectiformes with documented differences in body size. Finally, our quantitative expression analysis, focusing on liver and muscle tissues, reveals that ghr1tr of P. adspersus was less expressed in bigger adult individuals. In this sense, this study proposes ghr1tr as the modulator of size dimorphism growth and that it could be a useful target for genetic studies on fine flounder breeding improvement.
Fil: Marín, Alan. Universidad Nacional del Santa; Perú
Fil: Alonso, Andrés Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Delgadin, Tomás H.. Universidad Nacional del Santa; Perú
Fil: López Landavery, Edgar A.. Universidad Nacional del Santa; Perú
Fil: Cometivos, Lise Jara. Universidad Nacional del Santa; Perú
Fil: Saavedra Flores, Anaid. Universidad Nacional del Santa; Perú
Fil: Reyes Flores, Lorenzo E.. Universidad Nacional del Santa; Perú
Fil: Yzásiga Barrera, Carmen G.. Universidad Nacional del Santa; Perú
Fil: Fernandino, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Zelada Mázmela, Eliana. Universidad Nacional del Santa; Perú
description Differences in body size within a fish population are a recurring problem in aquaculture. In fish farms, a higher growth rate is one of the most important selection traits, so growth dispersion with smaller batch sizes leads to increased production costs. Despite the importance of this dispersion in growth, the understanding of the molecular basis of these differences is still largely unknown. In the present work, we firstly determined that in the fine flounder Paralichthys adspersus the variant of the growth hormone receptor 1 (ghr1tr) is in agreement with a transcript truncation product of an alternative polyadenylation signal (APAs) embedded in an intronic region of ghr1 full length nucleotide sequence. Additionally, we observed that this region possesses a high identity with homologous regions in other Pleuronectiformes with documented differences in body size. Finally, our quantitative expression analysis, focusing on liver and muscle tissues, reveals that ghr1tr of P. adspersus was less expressed in bigger adult individuals. In this sense, this study proposes ghr1tr as the modulator of size dimorphism growth and that it could be a useful target for genetic studies on fine flounder breeding improvement.
publishDate 2023
dc.date.none.fl_str_mv 2023-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/221829
Marín, Alan; Alonso, Andrés Mariano; Delgadin, Tomás H.; López Landavery, Edgar A.; Cometivos, Lise Jara; et al.; Analysis of truncated growth hormone receptor 1 in the differential growth of fine flounder Paralichthys adspersus; Elsevier Science; Aquaculture; 574; 9-2023; 1-8
0044-8486
CONICET Digital
CONICET
url http://hdl.handle.net/11336/221829
identifier_str_mv Marín, Alan; Alonso, Andrés Mariano; Delgadin, Tomás H.; López Landavery, Edgar A.; Cometivos, Lise Jara; et al.; Analysis of truncated growth hormone receptor 1 in the differential growth of fine flounder Paralichthys adspersus; Elsevier Science; Aquaculture; 574; 9-2023; 1-8
0044-8486
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0044848623004659
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2023.739691
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842981360866689024
score 12.48226

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