Evaluation of the infectivity and the persistence of Trichinella patagoniensis in muscle tissue of decomposing guinea pig (Cavia porcellus)

Autores
Fariña, Fernando Adrián; Pasqualetti, Mariana Ines; Ilgová, J.; Cardillo, Natalia Marina; Ercole, Mariano Emmanuel; Aronowicz, Tatiana; Krivokapich, Silvio Jesús; Kasný, M.; Ribicich, Miriam Mabel
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Trichinella patagoniensis, a new species of Trichinella, is widespread in Argentina. The success of parasite transmission depends, among other factors, on the resistance of L1 larvae present in the muscle tissue (ML) of dead hosts undergoing the decomposition process in different environmental conditions. The aim of the present work was to study the infectivity of T. patagoniensis muscle larvae in Cavia porcellus and the capability of the parasite to survive in decomposed muscle tissue of guinea pigs subjected to different environmental conditions. Thirty-two female Ssi:AL guinea pigs were orally inoculated with 2000 ML of T. patagoniensis (ISS2311). All the animals were sacrificed 42 days post-infection. Twenty-six animals were eviscerated, and carcasses were placed on the surface of soil inside plastic boxes that were exposed to environmental conditions in the summer 2014–2015 and autumn of 2015 in Buenos Aires, Argentina. Carcasses from six animals were placed into a plastic box inside the refrigerator at a temperature of 4 °C. The muscle tissue samples from the carcasses were examined weekly for the presence of larvae, and the infectivity of recovered ML was tested in BALB/c mice. Our results showed for the first time the ability of T. patagoniensis to complete its life cycle in guinea pigs, thus serving as a potential natural host. Also, larvae of T. patagoniensis remained infective in muscle tissue for several weeks while undergoing decomposition under different environmental conditions.
Fil: Fariña, Fernando Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Pasqualetti, Mariana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Ilgová, J.. Masaryk University; República Checa
Fil: Cardillo, Natalia Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Ercole, Mariano Emmanuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Aronowicz, Tatiana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Krivokapich, Silvio Jesús. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Kasný, M.. Masaryk University; República Checa. Charles University in Prague; República Checa
Fil: Ribicich, Miriam Mabel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Materia
Cavia Porcellus
Guinea Pigs
Infectivity
Persistence in Decomposing Muscle Tissue
Trichinella Patagoniensis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/64474
Acceder
id CONICETDig_9607e975be1a9ab3d0c294efdca74ff5
oai_identifier_str oai:ri.conicet.gov.ar:11336/64474
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of the infectivity and the persistence of Trichinella patagoniensis in muscle tissue of decomposing guinea pig (Cavia porcellus)Fariña, Fernando AdriánPasqualetti, Mariana InesIlgová, J.Cardillo, Natalia MarinaErcole, Mariano EmmanuelAronowicz, TatianaKrivokapich, Silvio JesúsKasný, M.Ribicich, Miriam MabelCavia PorcellusGuinea PigsInfectivityPersistence in Decomposing Muscle TissueTrichinella Patagoniensishttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Trichinella patagoniensis, a new species of Trichinella, is widespread in Argentina. The success of parasite transmission depends, among other factors, on the resistance of L1 larvae present in the muscle tissue (ML) of dead hosts undergoing the decomposition process in different environmental conditions. The aim of the present work was to study the infectivity of T. patagoniensis muscle larvae in Cavia porcellus and the capability of the parasite to survive in decomposed muscle tissue of guinea pigs subjected to different environmental conditions. Thirty-two female Ssi:AL guinea pigs were orally inoculated with 2000 ML of T. patagoniensis (ISS2311). All the animals were sacrificed 42 days post-infection. Twenty-six animals were eviscerated, and carcasses were placed on the surface of soil inside plastic boxes that were exposed to environmental conditions in the summer 2014–2015 and autumn of 2015 in Buenos Aires, Argentina. Carcasses from six animals were placed into a plastic box inside the refrigerator at a temperature of 4 °C. The muscle tissue samples from the carcasses were examined weekly for the presence of larvae, and the infectivity of recovered ML was tested in BALB/c mice. Our results showed for the first time the ability of T. patagoniensis to complete its life cycle in guinea pigs, thus serving as a potential natural host. Also, larvae of T. patagoniensis remained infective in muscle tissue for several weeks while undergoing decomposition under different environmental conditions.Fil: Fariña, Fernando Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Pasqualetti, Mariana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Ilgová, J.. Masaryk University; República ChecaFil: Cardillo, Natalia Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Ercole, Mariano Emmanuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Aronowicz, Tatiana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Krivokapich, Silvio Jesús. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaFil: Kasný, M.. Masaryk University; República Checa. Charles University in Prague; República ChecaFil: Ribicich, Miriam Mabel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaSpringer2017-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/64474Fariña, Fernando Adrián; Pasqualetti, Mariana Ines; Ilgová, J.; Cardillo, Natalia Marina; Ercole, Mariano Emmanuel; et al.; Evaluation of the infectivity and the persistence of Trichinella patagoniensis in muscle tissue of decomposing guinea pig (Cavia porcellus); Springer; Parasitology Research; 116; 1; 1-2017; 371-3750932-0113CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00436-016-5299-4info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00436-016-5299-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:21:58Zoai:ri.conicet.gov.ar:11336/64474instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:21:58.77CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of the infectivity and the persistence of Trichinella patagoniensis in muscle tissue of decomposing guinea pig (Cavia porcellus)
title Evaluation of the infectivity and the persistence of Trichinella patagoniensis in muscle tissue of decomposing guinea pig (Cavia porcellus)
spellingShingle Evaluation of the infectivity and the persistence of Trichinella patagoniensis in muscle tissue of decomposing guinea pig (Cavia porcellus)
Fariña, Fernando Adrián
Cavia Porcellus
Guinea Pigs
Infectivity
Persistence in Decomposing Muscle Tissue
Trichinella Patagoniensis
title_short Evaluation of the infectivity and the persistence of Trichinella patagoniensis in muscle tissue of decomposing guinea pig (Cavia porcellus)
title_full Evaluation of the infectivity and the persistence of Trichinella patagoniensis in muscle tissue of decomposing guinea pig (Cavia porcellus)
title_fullStr Evaluation of the infectivity and the persistence of Trichinella patagoniensis in muscle tissue of decomposing guinea pig (Cavia porcellus)
title_full_unstemmed Evaluation of the infectivity and the persistence of Trichinella patagoniensis in muscle tissue of decomposing guinea pig (Cavia porcellus)
title_sort Evaluation of the infectivity and the persistence of Trichinella patagoniensis in muscle tissue of decomposing guinea pig (Cavia porcellus)
dc.creator.none.fl_str_mv Fariña, Fernando Adrián
Pasqualetti, Mariana Ines
Ilgová, J.
Cardillo, Natalia Marina
Ercole, Mariano Emmanuel
Aronowicz, Tatiana
Krivokapich, Silvio Jesús
Kasný, M.
Ribicich, Miriam Mabel
author Fariña, Fernando Adrián
author_facet Fariña, Fernando Adrián
Pasqualetti, Mariana Ines
Ilgová, J.
Cardillo, Natalia Marina
Ercole, Mariano Emmanuel
Aronowicz, Tatiana
Krivokapich, Silvio Jesús
Kasný, M.
Ribicich, Miriam Mabel
author_role author
author2 Pasqualetti, Mariana Ines
Ilgová, J.
Cardillo, Natalia Marina
Ercole, Mariano Emmanuel
Aronowicz, Tatiana
Krivokapich, Silvio Jesús
Kasný, M.
Ribicich, Miriam Mabel
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cavia Porcellus
Guinea Pigs
Infectivity
Persistence in Decomposing Muscle Tissue
Trichinella Patagoniensis
topic Cavia Porcellus
Guinea Pigs
Infectivity
Persistence in Decomposing Muscle Tissue
Trichinella Patagoniensis
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Trichinella patagoniensis, a new species of Trichinella, is widespread in Argentina. The success of parasite transmission depends, among other factors, on the resistance of L1 larvae present in the muscle tissue (ML) of dead hosts undergoing the decomposition process in different environmental conditions. The aim of the present work was to study the infectivity of T. patagoniensis muscle larvae in Cavia porcellus and the capability of the parasite to survive in decomposed muscle tissue of guinea pigs subjected to different environmental conditions. Thirty-two female Ssi:AL guinea pigs were orally inoculated with 2000 ML of T. patagoniensis (ISS2311). All the animals were sacrificed 42 days post-infection. Twenty-six animals were eviscerated, and carcasses were placed on the surface of soil inside plastic boxes that were exposed to environmental conditions in the summer 2014–2015 and autumn of 2015 in Buenos Aires, Argentina. Carcasses from six animals were placed into a plastic box inside the refrigerator at a temperature of 4 °C. The muscle tissue samples from the carcasses were examined weekly for the presence of larvae, and the infectivity of recovered ML was tested in BALB/c mice. Our results showed for the first time the ability of T. patagoniensis to complete its life cycle in guinea pigs, thus serving as a potential natural host. Also, larvae of T. patagoniensis remained infective in muscle tissue for several weeks while undergoing decomposition under different environmental conditions.
Fil: Fariña, Fernando Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Pasqualetti, Mariana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Ilgová, J.. Masaryk University; República Checa
Fil: Cardillo, Natalia Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Ercole, Mariano Emmanuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Aronowicz, Tatiana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Krivokapich, Silvio Jesús. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Kasný, M.. Masaryk University; República Checa. Charles University in Prague; República Checa
Fil: Ribicich, Miriam Mabel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
description Trichinella patagoniensis, a new species of Trichinella, is widespread in Argentina. The success of parasite transmission depends, among other factors, on the resistance of L1 larvae present in the muscle tissue (ML) of dead hosts undergoing the decomposition process in different environmental conditions. The aim of the present work was to study the infectivity of T. patagoniensis muscle larvae in Cavia porcellus and the capability of the parasite to survive in decomposed muscle tissue of guinea pigs subjected to different environmental conditions. Thirty-two female Ssi:AL guinea pigs were orally inoculated with 2000 ML of T. patagoniensis (ISS2311). All the animals were sacrificed 42 days post-infection. Twenty-six animals were eviscerated, and carcasses were placed on the surface of soil inside plastic boxes that were exposed to environmental conditions in the summer 2014–2015 and autumn of 2015 in Buenos Aires, Argentina. Carcasses from six animals were placed into a plastic box inside the refrigerator at a temperature of 4 °C. The muscle tissue samples from the carcasses were examined weekly for the presence of larvae, and the infectivity of recovered ML was tested in BALB/c mice. Our results showed for the first time the ability of T. patagoniensis to complete its life cycle in guinea pigs, thus serving as a potential natural host. Also, larvae of T. patagoniensis remained infective in muscle tissue for several weeks while undergoing decomposition under different environmental conditions.
publishDate 2017
dc.date.none.fl_str_mv 2017-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/64474
Fariña, Fernando Adrián; Pasqualetti, Mariana Ines; Ilgová, J.; Cardillo, Natalia Marina; Ercole, Mariano Emmanuel; et al.; Evaluation of the infectivity and the persistence of Trichinella patagoniensis in muscle tissue of decomposing guinea pig (Cavia porcellus); Springer; Parasitology Research; 116; 1; 1-2017; 371-375
0932-0113
CONICET Digital
CONICET
url http://hdl.handle.net/11336/64474
identifier_str_mv Fariña, Fernando Adrián; Pasqualetti, Mariana Ines; Ilgová, J.; Cardillo, Natalia Marina; Ercole, Mariano Emmanuel; et al.; Evaluation of the infectivity and the persistence of Trichinella patagoniensis in muscle tissue of decomposing guinea pig (Cavia porcellus); Springer; Parasitology Research; 116; 1; 1-2017; 371-375
0932-0113
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00436-016-5299-4
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00436-016-5299-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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