Histamine protects bone marrow against cellular damage induced by ionising radiation

Autores
Medina, Vanina Araceli; Croci, Máximo; Carabajal, Eliana; Bergoc, Rosa Maria; Rivera, Elena S.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Purpose: Based on our previous data on the histamine radioprotective effect on small intestine, in the present work we aimed to determine whether histamine is able to protect bone marrow cells against ionising radiation damage. Materials and methods: 56 mice and 40 rats were divided into four groups. Histamine and histamine-irradiated groups received a daily subcutaneous histamine injection (0.1 mg/kg) starting 24 h before irradiation. Irradiated groups received a single dose on whole-body using Cesium-137 source and were sacrificed three days after irradiation. We evaluated the number of medullar components, bone marrow trophism, oedema, vascular damage, and other histological characteristics and also proliferation markers by immunohistochemistry. Results: Histamine treatment substantially reduced the grade of aplasia, the oedema and vascular damage induced by ionising radiation on bone marrow of mice and rats. Additionally, histamine preserved medullar components increasing the number of megakaryocytes (14.0 ± 1.0 vs. 7.3 ± 1.0 in mice; and 9.9 ± 1.3 vs. 4.1 ± 1.0 in rats, P < 0.01) and also myeloid (253.4 ± 37.6 vs. 7.8 ± 1.5 in mice; and 52.0 ± 3.7 vs. 31.8 ± 3.1 in rats, P < 0.01), lymphoid (97.4 ± 6.5 vs. 19.8 ± 1.6 in mice; and 23.4 ± 0.9 vs. 11.7 ± 2.5 in rats, P < 0.01) and erythroid cells (165.0 ± 9.1 vs. 8.8 ± 2.8 in mice; and 27.3 ± 2.3 vs. 15.6 ± 3.5 in rats, P < 0.01) per mm2. This effect was associated with an increased proliferation rate of bone marrow cells. Conclusions: Histamine reduces ionising radiation toxicity on bone marrow cells being a suitable candidate for use as radioprotector, especially for patients undergoing radiotherapy who are at the risk of bone marrow or small intestine damage.
Fil: Medina, Vanina Araceli. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Croci, Máximo. Instituto de Inmuno Oncología Dr. Ernesto J. V. Crescenti; Argentina
Fil: Carabajal, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Bergoc, Rosa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Rivera, Elena S.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Materia
Histamine
Ionising Radiation
Radioprotection
Proliferation
Bone Narrow
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14198
Acceder
id CONICETDig_f776b6a8a3805829ccd71e95a2fad1db
oai_identifier_str oai:ri.conicet.gov.ar:11336/14198
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Histamine protects bone marrow against cellular damage induced by ionising radiationMedina, Vanina AraceliCroci, MáximoCarabajal, ElianaBergoc, Rosa MariaRivera, Elena S.HistamineIonising RadiationRadioprotectionProliferationBone Narrowhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Purpose: Based on our previous data on the histamine radioprotective effect on small intestine, in the present work we aimed to determine whether histamine is able to protect bone marrow cells against ionising radiation damage. Materials and methods: 56 mice and 40 rats were divided into four groups. Histamine and histamine-irradiated groups received a daily subcutaneous histamine injection (0.1 mg/kg) starting 24 h before irradiation. Irradiated groups received a single dose on whole-body using Cesium-137 source and were sacrificed three days after irradiation. We evaluated the number of medullar components, bone marrow trophism, oedema, vascular damage, and other histological characteristics and also proliferation markers by immunohistochemistry. Results: Histamine treatment substantially reduced the grade of aplasia, the oedema and vascular damage induced by ionising radiation on bone marrow of mice and rats. Additionally, histamine preserved medullar components increasing the number of megakaryocytes (14.0 ± 1.0 vs. 7.3 ± 1.0 in mice; and 9.9 ± 1.3 vs. 4.1 ± 1.0 in rats, P < 0.01) and also myeloid (253.4 ± 37.6 vs. 7.8 ± 1.5 in mice; and 52.0 ± 3.7 vs. 31.8 ± 3.1 in rats, P < 0.01), lymphoid (97.4 ± 6.5 vs. 19.8 ± 1.6 in mice; and 23.4 ± 0.9 vs. 11.7 ± 2.5 in rats, P < 0.01) and erythroid cells (165.0 ± 9.1 vs. 8.8 ± 2.8 in mice; and 27.3 ± 2.3 vs. 15.6 ± 3.5 in rats, P < 0.01) per mm2. This effect was associated with an increased proliferation rate of bone marrow cells. Conclusions: Histamine reduces ionising radiation toxicity on bone marrow cells being a suitable candidate for use as radioprotector, especially for patients undergoing radiotherapy who are at the risk of bone marrow or small intestine damage.Fil: Medina, Vanina Araceli. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Croci, Máximo. Instituto de Inmuno Oncología Dr. Ernesto J. V. Crescenti; ArgentinaFil: Carabajal, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Bergoc, Rosa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Rivera, Elena S.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaTaylor & Francis2010-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14198Medina, Vanina Araceli; Croci, Máximo; Carabajal, Eliana; Bergoc, Rosa Maria; Rivera, Elena S.; Histamine protects bone marrow against cellular damage induced by ionising radiation; Taylor & Francis; International Journal Of Radiation Biology; 86; 4; 4-2010; 283-2900955-30021362-3095enginfo:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.3109/09553000903564067info:eu-repo/semantics/altIdentifier/doi/10.3109/09553000903564067info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:48Zoai:ri.conicet.gov.ar:11336/14198instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:48.487CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Histamine protects bone marrow against cellular damage induced by ionising radiation
title Histamine protects bone marrow against cellular damage induced by ionising radiation
spellingShingle Histamine protects bone marrow against cellular damage induced by ionising radiation
Medina, Vanina Araceli
Histamine
Ionising Radiation
Radioprotection
Proliferation
Bone Narrow
title_short Histamine protects bone marrow against cellular damage induced by ionising radiation
title_full Histamine protects bone marrow against cellular damage induced by ionising radiation
title_fullStr Histamine protects bone marrow against cellular damage induced by ionising radiation
title_full_unstemmed Histamine protects bone marrow against cellular damage induced by ionising radiation
title_sort Histamine protects bone marrow against cellular damage induced by ionising radiation
dc.creator.none.fl_str_mv Medina, Vanina Araceli
Croci, Máximo
Carabajal, Eliana
Bergoc, Rosa Maria
Rivera, Elena S.
author Medina, Vanina Araceli
author_facet Medina, Vanina Araceli
Croci, Máximo
Carabajal, Eliana
Bergoc, Rosa Maria
Rivera, Elena S.
author_role author
author2 Croci, Máximo
Carabajal, Eliana
Bergoc, Rosa Maria
Rivera, Elena S.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Histamine
Ionising Radiation
Radioprotection
Proliferation
Bone Narrow
topic Histamine
Ionising Radiation
Radioprotection
Proliferation
Bone Narrow
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Purpose: Based on our previous data on the histamine radioprotective effect on small intestine, in the present work we aimed to determine whether histamine is able to protect bone marrow cells against ionising radiation damage. Materials and methods: 56 mice and 40 rats were divided into four groups. Histamine and histamine-irradiated groups received a daily subcutaneous histamine injection (0.1 mg/kg) starting 24 h before irradiation. Irradiated groups received a single dose on whole-body using Cesium-137 source and were sacrificed three days after irradiation. We evaluated the number of medullar components, bone marrow trophism, oedema, vascular damage, and other histological characteristics and also proliferation markers by immunohistochemistry. Results: Histamine treatment substantially reduced the grade of aplasia, the oedema and vascular damage induced by ionising radiation on bone marrow of mice and rats. Additionally, histamine preserved medullar components increasing the number of megakaryocytes (14.0 ± 1.0 vs. 7.3 ± 1.0 in mice; and 9.9 ± 1.3 vs. 4.1 ± 1.0 in rats, P < 0.01) and also myeloid (253.4 ± 37.6 vs. 7.8 ± 1.5 in mice; and 52.0 ± 3.7 vs. 31.8 ± 3.1 in rats, P < 0.01), lymphoid (97.4 ± 6.5 vs. 19.8 ± 1.6 in mice; and 23.4 ± 0.9 vs. 11.7 ± 2.5 in rats, P < 0.01) and erythroid cells (165.0 ± 9.1 vs. 8.8 ± 2.8 in mice; and 27.3 ± 2.3 vs. 15.6 ± 3.5 in rats, P < 0.01) per mm2. This effect was associated with an increased proliferation rate of bone marrow cells. Conclusions: Histamine reduces ionising radiation toxicity on bone marrow cells being a suitable candidate for use as radioprotector, especially for patients undergoing radiotherapy who are at the risk of bone marrow or small intestine damage.
Fil: Medina, Vanina Araceli. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Croci, Máximo. Instituto de Inmuno Oncología Dr. Ernesto J. V. Crescenti; Argentina
Fil: Carabajal, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Bergoc, Rosa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Rivera, Elena S.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
description Purpose: Based on our previous data on the histamine radioprotective effect on small intestine, in the present work we aimed to determine whether histamine is able to protect bone marrow cells against ionising radiation damage. Materials and methods: 56 mice and 40 rats were divided into four groups. Histamine and histamine-irradiated groups received a daily subcutaneous histamine injection (0.1 mg/kg) starting 24 h before irradiation. Irradiated groups received a single dose on whole-body using Cesium-137 source and were sacrificed three days after irradiation. We evaluated the number of medullar components, bone marrow trophism, oedema, vascular damage, and other histological characteristics and also proliferation markers by immunohistochemistry. Results: Histamine treatment substantially reduced the grade of aplasia, the oedema and vascular damage induced by ionising radiation on bone marrow of mice and rats. Additionally, histamine preserved medullar components increasing the number of megakaryocytes (14.0 ± 1.0 vs. 7.3 ± 1.0 in mice; and 9.9 ± 1.3 vs. 4.1 ± 1.0 in rats, P < 0.01) and also myeloid (253.4 ± 37.6 vs. 7.8 ± 1.5 in mice; and 52.0 ± 3.7 vs. 31.8 ± 3.1 in rats, P < 0.01), lymphoid (97.4 ± 6.5 vs. 19.8 ± 1.6 in mice; and 23.4 ± 0.9 vs. 11.7 ± 2.5 in rats, P < 0.01) and erythroid cells (165.0 ± 9.1 vs. 8.8 ± 2.8 in mice; and 27.3 ± 2.3 vs. 15.6 ± 3.5 in rats, P < 0.01) per mm2. This effect was associated with an increased proliferation rate of bone marrow cells. Conclusions: Histamine reduces ionising radiation toxicity on bone marrow cells being a suitable candidate for use as radioprotector, especially for patients undergoing radiotherapy who are at the risk of bone marrow or small intestine damage.
publishDate 2010
dc.date.none.fl_str_mv 2010-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14198
Medina, Vanina Araceli; Croci, Máximo; Carabajal, Eliana; Bergoc, Rosa Maria; Rivera, Elena S.; Histamine protects bone marrow against cellular damage induced by ionising radiation; Taylor & Francis; International Journal Of Radiation Biology; 86; 4; 4-2010; 283-290
0955-3002
1362-3095
url http://hdl.handle.net/11336/14198
identifier_str_mv Medina, Vanina Araceli; Croci, Máximo; Carabajal, Eliana; Bergoc, Rosa Maria; Rivera, Elena S.; Histamine protects bone marrow against cellular damage induced by ionising radiation; Taylor & Francis; International Journal Of Radiation Biology; 86; 4; 4-2010; 283-290
0955-3002
1362-3095
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.3109/09553000903564067
info:eu-repo/semantics/altIdentifier/doi/10.3109/09553000903564067
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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