Effect of three non-protein thiols on CHO cells exposed to low doses of X-radiation

Autores
de Luca, Julio Cesar; Lopez Larraza, Daniel Mario
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The goals of this study were to determine whether cysteamine, β-mercaptohetanol and glutathione protect CHO K1 cells against chromosomal aberrations induced by low doses of X-radiation, as well as whether radiation protection is related to physical properties of those thiols. Experimental design included four different treatments: (a) control, (b) cells treated with 5 mM of each thiol, (c) cells treated with 100 mGy of X-rays and (4) cells treated with 5 mM of each thiol and 100 mGy X-rays. In combined treatment, all thiols were added 30 min before irradiation. Thiols remained until cell sacrifice (18 h). Maximum protection was afforded by cysteamine, the minimum protection was produced by glutathione while β-mercaptohetanol did not sow radioprotection effect. These results are consistent with the electrical charges and chemical structure of the three thiols and might be explained by the lower or higher access to DNA.
Fil: de Luca, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Lopez Larraza, Daniel Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto Multidisciplinario de Biología Celular (i); Argentina
Materia
Chromosome Aberrations
Ionising Radiation
Cho K1 Cells
Radioprotection
Β-Mercaptohetanol
Thiol Compounds
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11626
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/11626
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of three non-protein thiols on CHO cells exposed to low doses of X-radiationde Luca, Julio CesarLopez Larraza, Daniel MarioChromosome AberrationsIonising RadiationCho K1 CellsRadioprotectionΒ-MercaptohetanolThiol Compoundshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The goals of this study were to determine whether cysteamine, β-mercaptohetanol and glutathione protect CHO K1 cells against chromosomal aberrations induced by low doses of X-radiation, as well as whether radiation protection is related to physical properties of those thiols. Experimental design included four different treatments: (a) control, (b) cells treated with 5 mM of each thiol, (c) cells treated with 100 mGy of X-rays and (4) cells treated with 5 mM of each thiol and 100 mGy X-rays. In combined treatment, all thiols were added 30 min before irradiation. Thiols remained until cell sacrifice (18 h). Maximum protection was afforded by cysteamine, the minimum protection was produced by glutathione while β-mercaptohetanol did not sow radioprotection effect. These results are consistent with the electrical charges and chemical structure of the three thiols and might be explained by the lower or higher access to DNA.Fil: de Luca, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Lopez Larraza, Daniel Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto Multidisciplinario de Biología Celular (i); ArgentinaInderscience2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11626de Luca, Julio Cesar; Lopez Larraza, Daniel Mario; Effect of three non-protein thiols on CHO cells exposed to low doses of X-radiation; Inderscience; International Journal of Low Radiation; 9; 2; -1-2013; 138-1461477-65451741-9190enginfo:eu-repo/semantics/altIdentifier/doi/10.1504/IJLR.2013.055607info:eu-repo/semantics/altIdentifier/url/http://www.inderscienceonline.com/doi/abs/10.1504/IJLR.2013.055607info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:08Zoai:ri.conicet.gov.ar:11336/11626instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:08.568CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of three non-protein thiols on CHO cells exposed to low doses of X-radiation
title Effect of three non-protein thiols on CHO cells exposed to low doses of X-radiation
spellingShingle Effect of three non-protein thiols on CHO cells exposed to low doses of X-radiation
de Luca, Julio Cesar
Chromosome Aberrations
Ionising Radiation
Cho K1 Cells
Radioprotection
Β-Mercaptohetanol
Thiol Compounds
title_short Effect of three non-protein thiols on CHO cells exposed to low doses of X-radiation
title_full Effect of three non-protein thiols on CHO cells exposed to low doses of X-radiation
title_fullStr Effect of three non-protein thiols on CHO cells exposed to low doses of X-radiation
title_full_unstemmed Effect of three non-protein thiols on CHO cells exposed to low doses of X-radiation
title_sort Effect of three non-protein thiols on CHO cells exposed to low doses of X-radiation
dc.creator.none.fl_str_mv de Luca, Julio Cesar
Lopez Larraza, Daniel Mario
author de Luca, Julio Cesar
author_facet de Luca, Julio Cesar
Lopez Larraza, Daniel Mario
author_role author
author2 Lopez Larraza, Daniel Mario
author2_role author
dc.subject.none.fl_str_mv Chromosome Aberrations
Ionising Radiation
Cho K1 Cells
Radioprotection
Β-Mercaptohetanol
Thiol Compounds
topic Chromosome Aberrations
Ionising Radiation
Cho K1 Cells
Radioprotection
Β-Mercaptohetanol
Thiol Compounds
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The goals of this study were to determine whether cysteamine, β-mercaptohetanol and glutathione protect CHO K1 cells against chromosomal aberrations induced by low doses of X-radiation, as well as whether radiation protection is related to physical properties of those thiols. Experimental design included four different treatments: (a) control, (b) cells treated with 5 mM of each thiol, (c) cells treated with 100 mGy of X-rays and (4) cells treated with 5 mM of each thiol and 100 mGy X-rays. In combined treatment, all thiols were added 30 min before irradiation. Thiols remained until cell sacrifice (18 h). Maximum protection was afforded by cysteamine, the minimum protection was produced by glutathione while β-mercaptohetanol did not sow radioprotection effect. These results are consistent with the electrical charges and chemical structure of the three thiols and might be explained by the lower or higher access to DNA.
Fil: de Luca, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria "Ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Lopez Larraza, Daniel Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto Multidisciplinario de Biología Celular (i); Argentina
description The goals of this study were to determine whether cysteamine, β-mercaptohetanol and glutathione protect CHO K1 cells against chromosomal aberrations induced by low doses of X-radiation, as well as whether radiation protection is related to physical properties of those thiols. Experimental design included four different treatments: (a) control, (b) cells treated with 5 mM of each thiol, (c) cells treated with 100 mGy of X-rays and (4) cells treated with 5 mM of each thiol and 100 mGy X-rays. In combined treatment, all thiols were added 30 min before irradiation. Thiols remained until cell sacrifice (18 h). Maximum protection was afforded by cysteamine, the minimum protection was produced by glutathione while β-mercaptohetanol did not sow radioprotection effect. These results are consistent with the electrical charges and chemical structure of the three thiols and might be explained by the lower or higher access to DNA.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11626
de Luca, Julio Cesar; Lopez Larraza, Daniel Mario; Effect of three non-protein thiols on CHO cells exposed to low doses of X-radiation; Inderscience; International Journal of Low Radiation; 9; 2; -1-2013; 138-146
1477-6545
1741-9190
url http://hdl.handle.net/11336/11626
identifier_str_mv de Luca, Julio Cesar; Lopez Larraza, Daniel Mario; Effect of three non-protein thiols on CHO cells exposed to low doses of X-radiation; Inderscience; International Journal of Low Radiation; 9; 2; -1-2013; 138-146
1477-6545
1741-9190
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1504/IJLR.2013.055607
info:eu-repo/semantics/altIdentifier/url/http://www.inderscienceonline.com/doi/abs/10.1504/IJLR.2013.055607
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Inderscience
publisher.none.fl_str_mv Inderscience
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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