Efficiency of antimicrobial photodynamic therapy with photodithazine® on mssa and mrsa strains

Autores
Nunes Souza, Beatriz Müller; Pinto, Juliana Guerra; Correia Pereira, André Henrique; Miñan, Alejandro Guillermo; Ferreira Strixino, Juliana
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Staphylococccus aureus is a ubiquitous and opportunistic bacteria associated with high mortality rates. Antimicrobial photodynamic therapy (aPDT) is based on the application of a light source and a photosensitizer that can interact with molecular oxygen, forming Reactive Oxygen Species (ROS) that result in bacterial inactivation. This study aimed to analyze, in vitro, the action of aPDT with Photodithazine® (PDZ) in methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) strains. The strains were incubated with PDZ at 25, 50, 75, and 100 mg/L for 15 min and irradiated with fluences of 25, 50, and 100 J/cm2 . The internalization of PDZ was evaluated by confocal microscopy, the bacterial growth by counting the number of colony-forming units, as well as the bacterial metabolic activity post-aPDT and the production of ROS. In both strains, the photosensitizer was internalized; the production of ROS increased when the aPDT was applied; there was a bacterial reduction compared to the control at all the evaluated fluences and concentrations; and, in most parameters, it was obtained complete inactivation with significant difference (p < 0.05). The implementation of aPDT with PDZ in clinical strains of S. aureus has resulted in its complete inactivation, including the MRSA strains.
Fil: Nunes Souza, Beatriz Müller. University of Vale do Paraíba; Brasil
Fil: Pinto, Juliana Guerra. University of Vale do Paraíba; Brasil
Fil: Correia Pereira, André Henrique. University of Vale do Paraíba; Brasil
Fil: Miñan, Alejandro Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Ferreira Strixino, Juliana. University of Vale do Paraíba; Brasil
Materia
ANTIMICROBIAL PHOTODYNAMIC THERAPY
MRSA
MSSA
PHOTODITHAZINE®
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/171675
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Efficiency of antimicrobial photodynamic therapy with photodithazine® on mssa and mrsa strainsNunes Souza, Beatriz MüllerPinto, Juliana GuerraCorreia Pereira, André HenriqueMiñan, Alejandro GuillermoFerreira Strixino, JulianaANTIMICROBIAL PHOTODYNAMIC THERAPYMRSAMSSAPHOTODITHAZINE®https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Staphylococccus aureus is a ubiquitous and opportunistic bacteria associated with high mortality rates. Antimicrobial photodynamic therapy (aPDT) is based on the application of a light source and a photosensitizer that can interact with molecular oxygen, forming Reactive Oxygen Species (ROS) that result in bacterial inactivation. This study aimed to analyze, in vitro, the action of aPDT with Photodithazine® (PDZ) in methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) strains. The strains were incubated with PDZ at 25, 50, 75, and 100 mg/L for 15 min and irradiated with fluences of 25, 50, and 100 J/cm2 . The internalization of PDZ was evaluated by confocal microscopy, the bacterial growth by counting the number of colony-forming units, as well as the bacterial metabolic activity post-aPDT and the production of ROS. In both strains, the photosensitizer was internalized; the production of ROS increased when the aPDT was applied; there was a bacterial reduction compared to the control at all the evaluated fluences and concentrations; and, in most parameters, it was obtained complete inactivation with significant difference (p < 0.05). The implementation of aPDT with PDZ in clinical strains of S. aureus has resulted in its complete inactivation, including the MRSA strains.Fil: Nunes Souza, Beatriz Müller. University of Vale do Paraíba; BrasilFil: Pinto, Juliana Guerra. University of Vale do Paraíba; BrasilFil: Correia Pereira, André Henrique. University of Vale do Paraíba; BrasilFil: Miñan, Alejandro Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Ferreira Strixino, Juliana. University of Vale do Paraíba; BrasilMultidisciplinary Digital Publishing Institute2021-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/171675Nunes Souza, Beatriz Müller; Pinto, Juliana Guerra; Correia Pereira, André Henrique; Miñan, Alejandro Guillermo; Ferreira Strixino, Juliana; Efficiency of antimicrobial photodynamic therapy with photodithazine® on mssa and mrsa strains; Multidisciplinary Digital Publishing Institute; Antibiotics; 10; 7; 7-2021; 1-172079-6382CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/antibiotics10070869info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2079-6382/10/7/869info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:12:49Zoai:ri.conicet.gov.ar:11336/171675instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:12:50.015CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Efficiency of antimicrobial photodynamic therapy with photodithazine® on mssa and mrsa strains
title Efficiency of antimicrobial photodynamic therapy with photodithazine® on mssa and mrsa strains
spellingShingle Efficiency of antimicrobial photodynamic therapy with photodithazine® on mssa and mrsa strains
Nunes Souza, Beatriz Müller
ANTIMICROBIAL PHOTODYNAMIC THERAPY
MRSA
MSSA
PHOTODITHAZINE®
title_short Efficiency of antimicrobial photodynamic therapy with photodithazine® on mssa and mrsa strains
title_full Efficiency of antimicrobial photodynamic therapy with photodithazine® on mssa and mrsa strains
title_fullStr Efficiency of antimicrobial photodynamic therapy with photodithazine® on mssa and mrsa strains
title_full_unstemmed Efficiency of antimicrobial photodynamic therapy with photodithazine® on mssa and mrsa strains
title_sort Efficiency of antimicrobial photodynamic therapy with photodithazine® on mssa and mrsa strains
dc.creator.none.fl_str_mv Nunes Souza, Beatriz Müller
Pinto, Juliana Guerra
Correia Pereira, André Henrique
Miñan, Alejandro Guillermo
Ferreira Strixino, Juliana
author Nunes Souza, Beatriz Müller
author_facet Nunes Souza, Beatriz Müller
Pinto, Juliana Guerra
Correia Pereira, André Henrique
Miñan, Alejandro Guillermo
Ferreira Strixino, Juliana
author_role author
author2 Pinto, Juliana Guerra
Correia Pereira, André Henrique
Miñan, Alejandro Guillermo
Ferreira Strixino, Juliana
author2_role author
author
author
author
dc.subject.none.fl_str_mv ANTIMICROBIAL PHOTODYNAMIC THERAPY
MRSA
MSSA
PHOTODITHAZINE®
topic ANTIMICROBIAL PHOTODYNAMIC THERAPY
MRSA
MSSA
PHOTODITHAZINE®
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Staphylococccus aureus is a ubiquitous and opportunistic bacteria associated with high mortality rates. Antimicrobial photodynamic therapy (aPDT) is based on the application of a light source and a photosensitizer that can interact with molecular oxygen, forming Reactive Oxygen Species (ROS) that result in bacterial inactivation. This study aimed to analyze, in vitro, the action of aPDT with Photodithazine® (PDZ) in methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) strains. The strains were incubated with PDZ at 25, 50, 75, and 100 mg/L for 15 min and irradiated with fluences of 25, 50, and 100 J/cm2 . The internalization of PDZ was evaluated by confocal microscopy, the bacterial growth by counting the number of colony-forming units, as well as the bacterial metabolic activity post-aPDT and the production of ROS. In both strains, the photosensitizer was internalized; the production of ROS increased when the aPDT was applied; there was a bacterial reduction compared to the control at all the evaluated fluences and concentrations; and, in most parameters, it was obtained complete inactivation with significant difference (p < 0.05). The implementation of aPDT with PDZ in clinical strains of S. aureus has resulted in its complete inactivation, including the MRSA strains.
Fil: Nunes Souza, Beatriz Müller. University of Vale do Paraíba; Brasil
Fil: Pinto, Juliana Guerra. University of Vale do Paraíba; Brasil
Fil: Correia Pereira, André Henrique. University of Vale do Paraíba; Brasil
Fil: Miñan, Alejandro Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Ferreira Strixino, Juliana. University of Vale do Paraíba; Brasil
description Staphylococccus aureus is a ubiquitous and opportunistic bacteria associated with high mortality rates. Antimicrobial photodynamic therapy (aPDT) is based on the application of a light source and a photosensitizer that can interact with molecular oxygen, forming Reactive Oxygen Species (ROS) that result in bacterial inactivation. This study aimed to analyze, in vitro, the action of aPDT with Photodithazine® (PDZ) in methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) strains. The strains were incubated with PDZ at 25, 50, 75, and 100 mg/L for 15 min and irradiated with fluences of 25, 50, and 100 J/cm2 . The internalization of PDZ was evaluated by confocal microscopy, the bacterial growth by counting the number of colony-forming units, as well as the bacterial metabolic activity post-aPDT and the production of ROS. In both strains, the photosensitizer was internalized; the production of ROS increased when the aPDT was applied; there was a bacterial reduction compared to the control at all the evaluated fluences and concentrations; and, in most parameters, it was obtained complete inactivation with significant difference (p < 0.05). The implementation of aPDT with PDZ in clinical strains of S. aureus has resulted in its complete inactivation, including the MRSA strains.
publishDate 2021
dc.date.none.fl_str_mv 2021-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/171675
Nunes Souza, Beatriz Müller; Pinto, Juliana Guerra; Correia Pereira, André Henrique; Miñan, Alejandro Guillermo; Ferreira Strixino, Juliana; Efficiency of antimicrobial photodynamic therapy with photodithazine® on mssa and mrsa strains; Multidisciplinary Digital Publishing Institute; Antibiotics; 10; 7; 7-2021; 1-17
2079-6382
CONICET Digital
CONICET
url http://hdl.handle.net/11336/171675
identifier_str_mv Nunes Souza, Beatriz Müller; Pinto, Juliana Guerra; Correia Pereira, André Henrique; Miñan, Alejandro Guillermo; Ferreira Strixino, Juliana; Efficiency of antimicrobial photodynamic therapy with photodithazine® on mssa and mrsa strains; Multidisciplinary Digital Publishing Institute; Antibiotics; 10; 7; 7-2021; 1-17
2079-6382
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/antibiotics10070869
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2079-6382/10/7/869
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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