Development of novel alginate lyase cross-linked aggregates for the oral treatment of cystic fibrosis

Autores
Islan, German Abel; Martinez, Nelida Yanina; Illanes, Andres; Castro, Guillermo Raul
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Alginate lyase (AL) from Sphingobacterium multivorum is unsuitable for oral delivery because of its rapid inactivation under acidic conditions. The synthesis of a novel crosslinking enzyme aggregate (CLEA) of AL (AL-CLEA) is proposed. AL precipitation with 95% ammonium sulfate and combined with low methoxylated pectin (LMP), showed 100% precipitation yield. Crosslinking with glutaraldehyde reduced the AL-CLEA activity to less than 1%, but addition of bovine serum albumin (BSA) and LMP during AL-CLEA synthesis increased the activity yield to 14.7%. AL-CLEA exposed to simulated gastric conditions (pH 1.2 to 3.0) showed more than 70% retention of enzymatic activity. Moreover, AL-CLEA showed thermal stability at temperatures over 37 °C. Stability against chemical denaturants (ethanol, acetone and propylene glycol) showed that AL-CLEA was 14 times more stable than free AL in all cases. Finally, a 25% viscosity reduction of alginate solution was achieved with AL-CLEA. This is the first report of AL-CLEA synthesis and evaluation.
Fil: Islan, German Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina
Fil: Martinez, Nelida Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina
Fil: Illanes, Andres. Pontificia Universidad Catolica de Valparaiso; Chile
Fil: Castro, Guillermo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina
Materia
Alginate Lyase
Cleas
Cystic Fibrosis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16056
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/16056
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of novel alginate lyase cross-linked aggregates for the oral treatment of cystic fibrosisIslan, German AbelMartinez, Nelida YaninaIllanes, AndresCastro, Guillermo RaulAlginate LyaseCleasCystic Fibrosishttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Alginate lyase (AL) from Sphingobacterium multivorum is unsuitable for oral delivery because of its rapid inactivation under acidic conditions. The synthesis of a novel crosslinking enzyme aggregate (CLEA) of AL (AL-CLEA) is proposed. AL precipitation with 95% ammonium sulfate and combined with low methoxylated pectin (LMP), showed 100% precipitation yield. Crosslinking with glutaraldehyde reduced the AL-CLEA activity to less than 1%, but addition of bovine serum albumin (BSA) and LMP during AL-CLEA synthesis increased the activity yield to 14.7%. AL-CLEA exposed to simulated gastric conditions (pH 1.2 to 3.0) showed more than 70% retention of enzymatic activity. Moreover, AL-CLEA showed thermal stability at temperatures over 37 °C. Stability against chemical denaturants (ethanol, acetone and propylene glycol) showed that AL-CLEA was 14 times more stable than free AL in all cases. Finally, a 25% viscosity reduction of alginate solution was achieved with AL-CLEA. This is the first report of AL-CLEA synthesis and evaluation.Fil: Islan, German Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; ArgentinaFil: Martinez, Nelida Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; ArgentinaFil: Illanes, Andres. Pontificia Universidad Catolica de Valparaiso; ChileFil: Castro, Guillermo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; ArgentinaRoyal Society of Chemistry2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16056Islan, German Abel; Martinez, Nelida Yanina; Illanes, Andres; Castro, Guillermo Raul; Development of novel alginate lyase cross-linked aggregates for the oral treatment of cystic fibrosis; Royal Society of Chemistry; RSC Advances; 4; 23; -1-2014; 11758-117652046-2069enginfo:eu-repo/semantics/altIdentifier/doi/ 10.1039/c3ra47850einfo:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2014/RA/c3ra47850e#!divAbstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:18:04Zoai:ri.conicet.gov.ar:11336/16056instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:18:05.113CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of novel alginate lyase cross-linked aggregates for the oral treatment of cystic fibrosis
title Development of novel alginate lyase cross-linked aggregates for the oral treatment of cystic fibrosis
spellingShingle Development of novel alginate lyase cross-linked aggregates for the oral treatment of cystic fibrosis
Islan, German Abel
Alginate Lyase
Cleas
Cystic Fibrosis
title_short Development of novel alginate lyase cross-linked aggregates for the oral treatment of cystic fibrosis
title_full Development of novel alginate lyase cross-linked aggregates for the oral treatment of cystic fibrosis
title_fullStr Development of novel alginate lyase cross-linked aggregates for the oral treatment of cystic fibrosis
title_full_unstemmed Development of novel alginate lyase cross-linked aggregates for the oral treatment of cystic fibrosis
title_sort Development of novel alginate lyase cross-linked aggregates for the oral treatment of cystic fibrosis
dc.creator.none.fl_str_mv Islan, German Abel
Martinez, Nelida Yanina
Illanes, Andres
Castro, Guillermo Raul
author Islan, German Abel
author_facet Islan, German Abel
Martinez, Nelida Yanina
Illanes, Andres
Castro, Guillermo Raul
author_role author
author2 Martinez, Nelida Yanina
Illanes, Andres
Castro, Guillermo Raul
author2_role author
author
author
dc.subject.none.fl_str_mv Alginate Lyase
Cleas
Cystic Fibrosis
topic Alginate Lyase
Cleas
Cystic Fibrosis
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Alginate lyase (AL) from Sphingobacterium multivorum is unsuitable for oral delivery because of its rapid inactivation under acidic conditions. The synthesis of a novel crosslinking enzyme aggregate (CLEA) of AL (AL-CLEA) is proposed. AL precipitation with 95% ammonium sulfate and combined with low methoxylated pectin (LMP), showed 100% precipitation yield. Crosslinking with glutaraldehyde reduced the AL-CLEA activity to less than 1%, but addition of bovine serum albumin (BSA) and LMP during AL-CLEA synthesis increased the activity yield to 14.7%. AL-CLEA exposed to simulated gastric conditions (pH 1.2 to 3.0) showed more than 70% retention of enzymatic activity. Moreover, AL-CLEA showed thermal stability at temperatures over 37 °C. Stability against chemical denaturants (ethanol, acetone and propylene glycol) showed that AL-CLEA was 14 times more stable than free AL in all cases. Finally, a 25% viscosity reduction of alginate solution was achieved with AL-CLEA. This is the first report of AL-CLEA synthesis and evaluation.
Fil: Islan, German Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina
Fil: Martinez, Nelida Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina
Fil: Illanes, Andres. Pontificia Universidad Catolica de Valparaiso; Chile
Fil: Castro, Guillermo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina
description Alginate lyase (AL) from Sphingobacterium multivorum is unsuitable for oral delivery because of its rapid inactivation under acidic conditions. The synthesis of a novel crosslinking enzyme aggregate (CLEA) of AL (AL-CLEA) is proposed. AL precipitation with 95% ammonium sulfate and combined with low methoxylated pectin (LMP), showed 100% precipitation yield. Crosslinking with glutaraldehyde reduced the AL-CLEA activity to less than 1%, but addition of bovine serum albumin (BSA) and LMP during AL-CLEA synthesis increased the activity yield to 14.7%. AL-CLEA exposed to simulated gastric conditions (pH 1.2 to 3.0) showed more than 70% retention of enzymatic activity. Moreover, AL-CLEA showed thermal stability at temperatures over 37 °C. Stability against chemical denaturants (ethanol, acetone and propylene glycol) showed that AL-CLEA was 14 times more stable than free AL in all cases. Finally, a 25% viscosity reduction of alginate solution was achieved with AL-CLEA. This is the first report of AL-CLEA synthesis and evaluation.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16056
Islan, German Abel; Martinez, Nelida Yanina; Illanes, Andres; Castro, Guillermo Raul; Development of novel alginate lyase cross-linked aggregates for the oral treatment of cystic fibrosis; Royal Society of Chemistry; RSC Advances; 4; 23; -1-2014; 11758-11765
2046-2069
url http://hdl.handle.net/11336/16056
identifier_str_mv Islan, German Abel; Martinez, Nelida Yanina; Illanes, Andres; Castro, Guillermo Raul; Development of novel alginate lyase cross-linked aggregates for the oral treatment of cystic fibrosis; Royal Society of Chemistry; RSC Advances; 4; 23; -1-2014; 11758-11765
2046-2069
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/ 10.1039/c3ra47850e
info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2014/RA/c3ra47850e#!divAbstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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