Alginate Lyase and Ciprofloxacin Co-Immobilization on Biopolymeric Microspheres for Cystic Fibrosis Treatment

Autores
Islan, German Abel; Bosio, Valeria Elizabeth; Castro, Guillermo Raul
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A new formulation is described based on biopolymeric microspheres containing alginate lyase (AL) and ciprofloxacin (Cip) for sustainable oral delivery in CF patients. Alginate (ALG) and high-methoxyl pectin (HMP) are selected as the biopolymers to develop a composite matrix. ALG microspheres coated with HMP and ALG-HMP blend are gelled in water/organic solvents mixtures, obtaining Cip encapsulations from 46.0 to 100.0%. ALG-HMP shows a Cip sustainable release profile and is able to encapsulate 90.0% of AL, showing 76.0% enzyme activity after release under simulated intestinal conditions. The developed system is a promising delivery carrier to treat chronic infection of Pseudomonas aeruginosa and to reduce the viscoelasticity of the mucus accumulated into intestine of CF patients.
Fil: Islan, German Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Bosio, Valeria Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Castro, Guillermo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Materia
Ciprofloxacin
Alginate Lyase
Cystic Fibrosis
Biopolymers
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4316
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Alginate Lyase and Ciprofloxacin Co-Immobilization on Biopolymeric Microspheres for Cystic Fibrosis TreatmentIslan, German AbelBosio, Valeria ElizabethCastro, Guillermo RaulCiprofloxacinAlginate LyaseCystic FibrosisBiopolymershttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2A new formulation is described based on biopolymeric microspheres containing alginate lyase (AL) and ciprofloxacin (Cip) for sustainable oral delivery in CF patients. Alginate (ALG) and high-methoxyl pectin (HMP) are selected as the biopolymers to develop a composite matrix. ALG microspheres coated with HMP and ALG-HMP blend are gelled in water/organic solvents mixtures, obtaining Cip encapsulations from 46.0 to 100.0%. ALG-HMP shows a Cip sustainable release profile and is able to encapsulate 90.0% of AL, showing 76.0% enzyme activity after release under simulated intestinal conditions. The developed system is a promising delivery carrier to treat chronic infection of Pseudomonas aeruginosa and to reduce the viscoelasticity of the mucus accumulated into intestine of CF patients.Fil: Islan, German Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaFil: Bosio, Valeria Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaFil: Castro, Guillermo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaWiley2013-08-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4316Islan, German Abel; Bosio, Valeria Elizabeth; Castro, Guillermo Raul; Alginate Lyase and Ciprofloxacin Co-Immobilization on Biopolymeric Microspheres for Cystic Fibrosis Treatment; Wiley; Macromolecular Bioscience; 13; 9; 21-8-2013; 1238-12481616-5187enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/mabi.201300134/abstractinfo:eu-repo/semantics/altIdentifier/doi/DOI:10.1002/mabi.201300134info:eu-repo/semantics/altIdentifier/issn/1616-5187info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:43:32Zoai:ri.conicet.gov.ar:11336/4316instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:43:32.865CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Alginate Lyase and Ciprofloxacin Co-Immobilization on Biopolymeric Microspheres for Cystic Fibrosis Treatment
title Alginate Lyase and Ciprofloxacin Co-Immobilization on Biopolymeric Microspheres for Cystic Fibrosis Treatment
spellingShingle Alginate Lyase and Ciprofloxacin Co-Immobilization on Biopolymeric Microspheres for Cystic Fibrosis Treatment
Islan, German Abel
Ciprofloxacin
Alginate Lyase
Cystic Fibrosis
Biopolymers
title_short Alginate Lyase and Ciprofloxacin Co-Immobilization on Biopolymeric Microspheres for Cystic Fibrosis Treatment
title_full Alginate Lyase and Ciprofloxacin Co-Immobilization on Biopolymeric Microspheres for Cystic Fibrosis Treatment
title_fullStr Alginate Lyase and Ciprofloxacin Co-Immobilization on Biopolymeric Microspheres for Cystic Fibrosis Treatment
title_full_unstemmed Alginate Lyase and Ciprofloxacin Co-Immobilization on Biopolymeric Microspheres for Cystic Fibrosis Treatment
title_sort Alginate Lyase and Ciprofloxacin Co-Immobilization on Biopolymeric Microspheres for Cystic Fibrosis Treatment
dc.creator.none.fl_str_mv Islan, German Abel
Bosio, Valeria Elizabeth
Castro, Guillermo Raul
author Islan, German Abel
author_facet Islan, German Abel
Bosio, Valeria Elizabeth
Castro, Guillermo Raul
author_role author
author2 Bosio, Valeria Elizabeth
Castro, Guillermo Raul
author2_role author
author
dc.subject.none.fl_str_mv Ciprofloxacin
Alginate Lyase
Cystic Fibrosis
Biopolymers
topic Ciprofloxacin
Alginate Lyase
Cystic Fibrosis
Biopolymers
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv A new formulation is described based on biopolymeric microspheres containing alginate lyase (AL) and ciprofloxacin (Cip) for sustainable oral delivery in CF patients. Alginate (ALG) and high-methoxyl pectin (HMP) are selected as the biopolymers to develop a composite matrix. ALG microspheres coated with HMP and ALG-HMP blend are gelled in water/organic solvents mixtures, obtaining Cip encapsulations from 46.0 to 100.0%. ALG-HMP shows a Cip sustainable release profile and is able to encapsulate 90.0% of AL, showing 76.0% enzyme activity after release under simulated intestinal conditions. The developed system is a promising delivery carrier to treat chronic infection of Pseudomonas aeruginosa and to reduce the viscoelasticity of the mucus accumulated into intestine of CF patients.
Fil: Islan, German Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Bosio, Valeria Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Castro, Guillermo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
description A new formulation is described based on biopolymeric microspheres containing alginate lyase (AL) and ciprofloxacin (Cip) for sustainable oral delivery in CF patients. Alginate (ALG) and high-methoxyl pectin (HMP) are selected as the biopolymers to develop a composite matrix. ALG microspheres coated with HMP and ALG-HMP blend are gelled in water/organic solvents mixtures, obtaining Cip encapsulations from 46.0 to 100.0%. ALG-HMP shows a Cip sustainable release profile and is able to encapsulate 90.0% of AL, showing 76.0% enzyme activity after release under simulated intestinal conditions. The developed system is a promising delivery carrier to treat chronic infection of Pseudomonas aeruginosa and to reduce the viscoelasticity of the mucus accumulated into intestine of CF patients.
publishDate 2013
dc.date.none.fl_str_mv 2013-08-21
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4316
Islan, German Abel; Bosio, Valeria Elizabeth; Castro, Guillermo Raul; Alginate Lyase and Ciprofloxacin Co-Immobilization on Biopolymeric Microspheres for Cystic Fibrosis Treatment; Wiley; Macromolecular Bioscience; 13; 9; 21-8-2013; 1238-1248
1616-5187
url http://hdl.handle.net/11336/4316
identifier_str_mv Islan, German Abel; Bosio, Valeria Elizabeth; Castro, Guillermo Raul; Alginate Lyase and Ciprofloxacin Co-Immobilization on Biopolymeric Microspheres for Cystic Fibrosis Treatment; Wiley; Macromolecular Bioscience; 13; 9; 21-8-2013; 1238-1248
1616-5187
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/mabi.201300134/abstract
info:eu-repo/semantics/altIdentifier/doi/DOI:10.1002/mabi.201300134
info:eu-repo/semantics/altIdentifier/issn/1616-5187
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.976206

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