Direct and indirect promotion of myelopoiesis mechanisms by postbiotics obtained from Lactobacillus rhamnosus CRL1505

Autores
Gutiérrez, Florencia; Vasile, Brenda Estefania; Ivir, Héctor Maximiliano; Alvarez, Gladis Susana; Salva, Maria Susana
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Many attempts have been made to find safer immunomodulatory agents that enhance the immune response and reduce the number and severity of infections in at-risk populations. Our previous studies have shown that Lactobacillus rhamnosus CRL1505 (Lr05) and its postbiotics, peptidoglycan (PG05) and cell wall (CW05), were able to improve bone marrow (BM) myelopiesis and to protect against respiratory pathogens in mice undergoing chemotherapy. However, the underlying mechanisms remain unknown. Hence, the role of TLR2 and G-CSF involved in the ability of Lr05, P05 and CW05 to induce basal myelopoiesis by direct or indirect interaction with BM hematopoietic stem and progenitor cells (HSPC) was evaluated. First, in vitro colony-forming unit assays were performed to assess whether the clonogenic capacity of BM cells responds to direct interaction with Lr05 and its postbiotics. For this, mouse BM cells were plated in the presence or absence of Lr05, PG05 or CW05 in culture medium for the granulocyte/macrophage forming unit (CFU-GM) (MethoCult? GFM3534). The counts and the phenotypic characterization of the colonies obtained were determined. Besides, the effect of the addition of fibroblast supernatants conditioned by Lr05 or its postbiotics on the clonogenic activity of HSPC was investigated. Finally, the expression of TLR2 of CFU-GM and the levels of G-CSF in the culture medium on day 14 were determined by flow cytometry and ELISA, respectively. Lr05 significantly stimulated the TLR2 expression and secretion of G-CSF, and enhanced the clonogenic activity of HSPC and fibroblast. Interestingly, CW05 showed a strong stimulatory effect while PG05 showed immune effects that were more similar to Lr05. These results allow us to know, at least in part, the cellular and molecular mechanisms involved in the myelopoiesis-enhancing capacity of new safe products to be potentially used in patients undergoing chemotherapeutic treatment.
Fil: Gutiérrez, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Vasile, Brenda Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Ivir, Héctor Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Reunión de Sociedades de Biociencias 2021; LXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión Anual de la Sociedad Argentina de Inmunología; LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental; XI Reunión Anual de la Asociación Argentina de Nanomedicinas
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Nanomedicinas
Materia
ACID LACTIC BACTERIA
POSTBIOTICS
CELULLAR CULTURE
CYTOKINES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/193405
Acceder
id CONICETDig_f6d995375cfc23b1b80f8c0491672f64
oai_identifier_str oai:ri.conicet.gov.ar:11336/193405
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Direct and indirect promotion of myelopoiesis mechanisms by postbiotics obtained from Lactobacillus rhamnosus CRL1505Gutiérrez, FlorenciaVasile, Brenda EstefaniaIvir, Héctor MaximilianoAlvarez, Gladis SusanaSalva, Maria SusanaACID LACTIC BACTERIAPOSTBIOTICSCELULLAR CULTURECYTOKINEShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Many attempts have been made to find safer immunomodulatory agents that enhance the immune response and reduce the number and severity of infections in at-risk populations. Our previous studies have shown that Lactobacillus rhamnosus CRL1505 (Lr05) and its postbiotics, peptidoglycan (PG05) and cell wall (CW05), were able to improve bone marrow (BM) myelopiesis and to protect against respiratory pathogens in mice undergoing chemotherapy. However, the underlying mechanisms remain unknown. Hence, the role of TLR2 and G-CSF involved in the ability of Lr05, P05 and CW05 to induce basal myelopoiesis by direct or indirect interaction with BM hematopoietic stem and progenitor cells (HSPC) was evaluated. First, in vitro colony-forming unit assays were performed to assess whether the clonogenic capacity of BM cells responds to direct interaction with Lr05 and its postbiotics. For this, mouse BM cells were plated in the presence or absence of Lr05, PG05 or CW05 in culture medium for the granulocyte/macrophage forming unit (CFU-GM) (MethoCult? GFM3534). The counts and the phenotypic characterization of the colonies obtained were determined. Besides, the effect of the addition of fibroblast supernatants conditioned by Lr05 or its postbiotics on the clonogenic activity of HSPC was investigated. Finally, the expression of TLR2 of CFU-GM and the levels of G-CSF in the culture medium on day 14 were determined by flow cytometry and ELISA, respectively. Lr05 significantly stimulated the TLR2 expression and secretion of G-CSF, and enhanced the clonogenic activity of HSPC and fibroblast. Interestingly, CW05 showed a strong stimulatory effect while PG05 showed immune effects that were more similar to Lr05. These results allow us to know, at least in part, the cellular and molecular mechanisms involved in the myelopoiesis-enhancing capacity of new safe products to be potentially used in patients undergoing chemotherapeutic treatment.Fil: Gutiérrez, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Vasile, Brenda Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Ivir, Héctor Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaReunión de Sociedades de Biociencias 2021; LXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión Anual de la Sociedad Argentina de Inmunología; LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental; XI Reunión Anual de la Asociación Argentina de NanomedicinasCiudad Autónoma de Buenos AiresArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaAsociación Argentina de Farmacología ExperimentalAsociación Argentina de NanomedicinasFundación Revista Medicina2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/193405Direct and indirect promotion of myelopoiesis mechanisms by postbiotics obtained from Lactobacillus rhamnosus CRL1505; Reunión de Sociedades de Biociencias 2021; LXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión Anual de la Sociedad Argentina de Inmunología; LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental; XI Reunión Anual de la Asociación Argentina de Nanomedicinas; Ciudad Autónoma de Buenos Aires; Argentina; 2021; 147-1470025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicinaNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:22:09Zoai:ri.conicet.gov.ar:11336/193405instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:22:09.696CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Direct and indirect promotion of myelopoiesis mechanisms by postbiotics obtained from Lactobacillus rhamnosus CRL1505
title Direct and indirect promotion of myelopoiesis mechanisms by postbiotics obtained from Lactobacillus rhamnosus CRL1505
spellingShingle Direct and indirect promotion of myelopoiesis mechanisms by postbiotics obtained from Lactobacillus rhamnosus CRL1505
Gutiérrez, Florencia
ACID LACTIC BACTERIA
POSTBIOTICS
CELULLAR CULTURE
CYTOKINES
title_short Direct and indirect promotion of myelopoiesis mechanisms by postbiotics obtained from Lactobacillus rhamnosus CRL1505
title_full Direct and indirect promotion of myelopoiesis mechanisms by postbiotics obtained from Lactobacillus rhamnosus CRL1505
title_fullStr Direct and indirect promotion of myelopoiesis mechanisms by postbiotics obtained from Lactobacillus rhamnosus CRL1505
title_full_unstemmed Direct and indirect promotion of myelopoiesis mechanisms by postbiotics obtained from Lactobacillus rhamnosus CRL1505
title_sort Direct and indirect promotion of myelopoiesis mechanisms by postbiotics obtained from Lactobacillus rhamnosus CRL1505
dc.creator.none.fl_str_mv Gutiérrez, Florencia
Vasile, Brenda Estefania
Ivir, Héctor Maximiliano
Alvarez, Gladis Susana
Salva, Maria Susana
author Gutiérrez, Florencia
author_facet Gutiérrez, Florencia
Vasile, Brenda Estefania
Ivir, Héctor Maximiliano
Alvarez, Gladis Susana
Salva, Maria Susana
author_role author
author2 Vasile, Brenda Estefania
Ivir, Héctor Maximiliano
Alvarez, Gladis Susana
Salva, Maria Susana
author2_role author
author
author
author
dc.subject.none.fl_str_mv ACID LACTIC BACTERIA
POSTBIOTICS
CELULLAR CULTURE
CYTOKINES
topic ACID LACTIC BACTERIA
POSTBIOTICS
CELULLAR CULTURE
CYTOKINES
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Many attempts have been made to find safer immunomodulatory agents that enhance the immune response and reduce the number and severity of infections in at-risk populations. Our previous studies have shown that Lactobacillus rhamnosus CRL1505 (Lr05) and its postbiotics, peptidoglycan (PG05) and cell wall (CW05), were able to improve bone marrow (BM) myelopiesis and to protect against respiratory pathogens in mice undergoing chemotherapy. However, the underlying mechanisms remain unknown. Hence, the role of TLR2 and G-CSF involved in the ability of Lr05, P05 and CW05 to induce basal myelopoiesis by direct or indirect interaction with BM hematopoietic stem and progenitor cells (HSPC) was evaluated. First, in vitro colony-forming unit assays were performed to assess whether the clonogenic capacity of BM cells responds to direct interaction with Lr05 and its postbiotics. For this, mouse BM cells were plated in the presence or absence of Lr05, PG05 or CW05 in culture medium for the granulocyte/macrophage forming unit (CFU-GM) (MethoCult? GFM3534). The counts and the phenotypic characterization of the colonies obtained were determined. Besides, the effect of the addition of fibroblast supernatants conditioned by Lr05 or its postbiotics on the clonogenic activity of HSPC was investigated. Finally, the expression of TLR2 of CFU-GM and the levels of G-CSF in the culture medium on day 14 were determined by flow cytometry and ELISA, respectively. Lr05 significantly stimulated the TLR2 expression and secretion of G-CSF, and enhanced the clonogenic activity of HSPC and fibroblast. Interestingly, CW05 showed a strong stimulatory effect while PG05 showed immune effects that were more similar to Lr05. These results allow us to know, at least in part, the cellular and molecular mechanisms involved in the myelopoiesis-enhancing capacity of new safe products to be potentially used in patients undergoing chemotherapeutic treatment.
Fil: Gutiérrez, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Vasile, Brenda Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Ivir, Héctor Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Reunión de Sociedades de Biociencias 2021; LXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión Anual de la Sociedad Argentina de Inmunología; LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental; XI Reunión Anual de la Asociación Argentina de Nanomedicinas
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Nanomedicinas
description Many attempts have been made to find safer immunomodulatory agents that enhance the immune response and reduce the number and severity of infections in at-risk populations. Our previous studies have shown that Lactobacillus rhamnosus CRL1505 (Lr05) and its postbiotics, peptidoglycan (PG05) and cell wall (CW05), were able to improve bone marrow (BM) myelopiesis and to protect against respiratory pathogens in mice undergoing chemotherapy. However, the underlying mechanisms remain unknown. Hence, the role of TLR2 and G-CSF involved in the ability of Lr05, P05 and CW05 to induce basal myelopoiesis by direct or indirect interaction with BM hematopoietic stem and progenitor cells (HSPC) was evaluated. First, in vitro colony-forming unit assays were performed to assess whether the clonogenic capacity of BM cells responds to direct interaction with Lr05 and its postbiotics. For this, mouse BM cells were plated in the presence or absence of Lr05, PG05 or CW05 in culture medium for the granulocyte/macrophage forming unit (CFU-GM) (MethoCult? GFM3534). The counts and the phenotypic characterization of the colonies obtained were determined. Besides, the effect of the addition of fibroblast supernatants conditioned by Lr05 or its postbiotics on the clonogenic activity of HSPC was investigated. Finally, the expression of TLR2 of CFU-GM and the levels of G-CSF in the culture medium on day 14 were determined by flow cytometry and ELISA, respectively. Lr05 significantly stimulated the TLR2 expression and secretion of G-CSF, and enhanced the clonogenic activity of HSPC and fibroblast. Interestingly, CW05 showed a strong stimulatory effect while PG05 showed immune effects that were more similar to Lr05. These results allow us to know, at least in part, the cellular and molecular mechanisms involved in the myelopoiesis-enhancing capacity of new safe products to be potentially used in patients undergoing chemotherapeutic treatment.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/193405
Direct and indirect promotion of myelopoiesis mechanisms by postbiotics obtained from Lactobacillus rhamnosus CRL1505; Reunión de Sociedades de Biociencias 2021; LXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión Anual de la Sociedad Argentina de Inmunología; LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental; XI Reunión Anual de la Asociación Argentina de Nanomedicinas; Ciudad Autónoma de Buenos Aires; Argentina; 2021; 147-147
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/193405
identifier_str_mv Direct and indirect promotion of myelopoiesis mechanisms by postbiotics obtained from Lactobacillus rhamnosus CRL1505; Reunión de Sociedades de Biociencias 2021; LXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión Anual de la Sociedad Argentina de Inmunología; LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental; XI Reunión Anual de la Asociación Argentina de Nanomedicinas; Ciudad Autónoma de Buenos Aires; Argentina; 2021; 147-147
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicina
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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