Effects of proanthocyanidin enriched extract from Ligaria cuneifolia on plasma cholesterol and hemorheological parameters. In vivo and In vitro studies

Autores
Dominighini, Alicia; Ferrero, Mariana Cristina; Crossetti, D.; Ronco, Maria Teresa; Gonzalves, Jose; Urli, Leda; Wagner, Marcelo Luis; Gurni, Alberto Angel; Carnovale, Cristina Ester; Luquita, Alejandra Nora
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
It was demonstrated that Ligaria cuneifolia (Lc) crude extract increased blood viscosity and decreased plasma cholesterol in rats. In the present study, we analyzed the Lc proanthocyanidin enriched fraction (PLc) to determine if it is capable of altering the hemorheological parameters while diminishing the plasma cholesterol. In vivo studies in adult male Wistar rats, randomized in three groups (n = 6 each one) were performed: 1. Control: saline intraperitoneal (i.p.); 2. PLc 0.6 mg/100 g body weight (b.w.) i.p. and 3. PLc 3 mg/100 g b.w. i.p., every 24 hours during 3 days. In vitro studies: with blood obtained by cardiac puncture, separated in aliquots and incubated with: 1. Saline solution (Control); 2. PLc 0.1 mg/mL, and 3. PLc 1.0 mg/mL, equivalent to doses in vivo experiments. The results demonstrated that in vivo PLc 0.6 and PLc 3 reduced plasma cholesterol (Cho) and LDL-Cho. Neither blood nor plasma viscosity was altered. Decrease of plasma cholesterol could be due to an increase of cholesterol and bile salts excretion leading to an increase of bile flow. In vitro experiments showed a direct interaction of PLc, at high concentration, with the erythrocyte membrane, inducing a switch from discocyte to stomatocyte. Only, PLc without hepatic metabolism produces hemorheological changes. Thus, PLc in vivo might be a pharmacological agent capable of decreasing plasma cholesterol.
Fil: Dominighini, Alicia. Universidad Nacional de Rosario. Facultad de Cs.medicas; Argentina
Fil: Ferrero, Mariana Cristina. Universidad Nacional de Rosario. Facultad de Cs.medicas; Argentina
Fil: Crossetti, D.. Universidad Nacional de Rosario. Facultad de Cs.medicas; Argentina
Fil: Ronco, Maria Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Fisiología Experimental (i); Argentina
Fil: Gonzalves, Jose. Universidad Nacional de Rosario. Facultad de Cs.medicas; Argentina
Fil: Urli, Leda. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Cs.fisiologicas; Argentina
Fil: Wagner, Marcelo Luis. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Gurni, Alberto Angel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Carnovale, Cristina Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Fisiología Experimental (i); Argentina
Fil: Luquita, Alejandra Nora. Universidad Nacional de Rosario. Facultad de Cs.medicas; Argentina
Materia
Proanthocyanidina
Cholesterol
Blood-Viscocity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/6102
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/6102
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of proanthocyanidin enriched extract from Ligaria cuneifolia on plasma cholesterol and hemorheological parameters. In vivo and In vitro studiesDominighini, AliciaFerrero, Mariana CristinaCrossetti, D.Ronco, Maria TeresaGonzalves, JoseUrli, LedaWagner, Marcelo LuisGurni, Alberto AngelCarnovale, Cristina EsterLuquita, Alejandra NoraProanthocyanidinaCholesterolBlood-Viscocityhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3It was demonstrated that Ligaria cuneifolia (Lc) crude extract increased blood viscosity and decreased plasma cholesterol in rats. In the present study, we analyzed the Lc proanthocyanidin enriched fraction (PLc) to determine if it is capable of altering the hemorheological parameters while diminishing the plasma cholesterol. In vivo studies in adult male Wistar rats, randomized in three groups (n = 6 each one) were performed: 1. Control: saline intraperitoneal (i.p.); 2. PLc 0.6 mg/100 g body weight (b.w.) i.p. and 3. PLc 3 mg/100 g b.w. i.p., every 24 hours during 3 days. In vitro studies: with blood obtained by cardiac puncture, separated in aliquots and incubated with: 1. Saline solution (Control); 2. PLc 0.1 mg/mL, and 3. PLc 1.0 mg/mL, equivalent to doses in vivo experiments. The results demonstrated that in vivo PLc 0.6 and PLc 3 reduced plasma cholesterol (Cho) and LDL-Cho. Neither blood nor plasma viscosity was altered. Decrease of plasma cholesterol could be due to an increase of cholesterol and bile salts excretion leading to an increase of bile flow. In vitro experiments showed a direct interaction of PLc, at high concentration, with the erythrocyte membrane, inducing a switch from discocyte to stomatocyte. Only, PLc without hepatic metabolism produces hemorheological changes. Thus, PLc in vivo might be a pharmacological agent capable of decreasing plasma cholesterol.Fil: Dominighini, Alicia. Universidad Nacional de Rosario. Facultad de Cs.medicas; ArgentinaFil: Ferrero, Mariana Cristina. Universidad Nacional de Rosario. Facultad de Cs.medicas; ArgentinaFil: Crossetti, D.. Universidad Nacional de Rosario. Facultad de Cs.medicas; ArgentinaFil: Ronco, Maria Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Fisiología Experimental (i); ArgentinaFil: Gonzalves, Jose. Universidad Nacional de Rosario. Facultad de Cs.medicas; ArgentinaFil: Urli, Leda. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Cs.fisiologicas; ArgentinaFil: Wagner, Marcelo Luis. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Gurni, Alberto Angel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Carnovale, Cristina Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Fisiología Experimental (i); ArgentinaFil: Luquita, Alejandra Nora. Universidad Nacional de Rosario. Facultad de Cs.medicas; ArgentinaIos Press2014-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6102Dominighini, Alicia; Ferrero, Mariana Cristina; Crossetti, D.; Ronco, Maria Teresa; Gonzalves, Jose; et al.; Effects of proanthocyanidin enriched extract from Ligaria cuneifolia on plasma cholesterol and hemorheological parameters. In vivo and In vitro studies; Ios Press; Clinical Hemorheology And Microcirculation; 60; 3; 8-2014; 317-3251386-0291enginfo:eu-repo/semantics/altIdentifier/url/http://content.iospress.com/articles/clinical-hemorheology-and-microcirculation/ch1870info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.3233/CH-141870info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:30:30Zoai:ri.conicet.gov.ar:11336/6102instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:30:30.56CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of proanthocyanidin enriched extract from Ligaria cuneifolia on plasma cholesterol and hemorheological parameters. In vivo and In vitro studies
title Effects of proanthocyanidin enriched extract from Ligaria cuneifolia on plasma cholesterol and hemorheological parameters. In vivo and In vitro studies
spellingShingle Effects of proanthocyanidin enriched extract from Ligaria cuneifolia on plasma cholesterol and hemorheological parameters. In vivo and In vitro studies
Dominighini, Alicia
Proanthocyanidina
Cholesterol
Blood-Viscocity
title_short Effects of proanthocyanidin enriched extract from Ligaria cuneifolia on plasma cholesterol and hemorheological parameters. In vivo and In vitro studies
title_full Effects of proanthocyanidin enriched extract from Ligaria cuneifolia on plasma cholesterol and hemorheological parameters. In vivo and In vitro studies
title_fullStr Effects of proanthocyanidin enriched extract from Ligaria cuneifolia on plasma cholesterol and hemorheological parameters. In vivo and In vitro studies
title_full_unstemmed Effects of proanthocyanidin enriched extract from Ligaria cuneifolia on plasma cholesterol and hemorheological parameters. In vivo and In vitro studies
title_sort Effects of proanthocyanidin enriched extract from Ligaria cuneifolia on plasma cholesterol and hemorheological parameters. In vivo and In vitro studies
dc.creator.none.fl_str_mv Dominighini, Alicia
Ferrero, Mariana Cristina
Crossetti, D.
Ronco, Maria Teresa
Gonzalves, Jose
Urli, Leda
Wagner, Marcelo Luis
Gurni, Alberto Angel
Carnovale, Cristina Ester
Luquita, Alejandra Nora
author Dominighini, Alicia
author_facet Dominighini, Alicia
Ferrero, Mariana Cristina
Crossetti, D.
Ronco, Maria Teresa
Gonzalves, Jose
Urli, Leda
Wagner, Marcelo Luis
Gurni, Alberto Angel
Carnovale, Cristina Ester
Luquita, Alejandra Nora
author_role author
author2 Ferrero, Mariana Cristina
Crossetti, D.
Ronco, Maria Teresa
Gonzalves, Jose
Urli, Leda
Wagner, Marcelo Luis
Gurni, Alberto Angel
Carnovale, Cristina Ester
Luquita, Alejandra Nora
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Proanthocyanidina
Cholesterol
Blood-Viscocity
topic Proanthocyanidina
Cholesterol
Blood-Viscocity
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv It was demonstrated that Ligaria cuneifolia (Lc) crude extract increased blood viscosity and decreased plasma cholesterol in rats. In the present study, we analyzed the Lc proanthocyanidin enriched fraction (PLc) to determine if it is capable of altering the hemorheological parameters while diminishing the plasma cholesterol. In vivo studies in adult male Wistar rats, randomized in three groups (n = 6 each one) were performed: 1. Control: saline intraperitoneal (i.p.); 2. PLc 0.6 mg/100 g body weight (b.w.) i.p. and 3. PLc 3 mg/100 g b.w. i.p., every 24 hours during 3 days. In vitro studies: with blood obtained by cardiac puncture, separated in aliquots and incubated with: 1. Saline solution (Control); 2. PLc 0.1 mg/mL, and 3. PLc 1.0 mg/mL, equivalent to doses in vivo experiments. The results demonstrated that in vivo PLc 0.6 and PLc 3 reduced plasma cholesterol (Cho) and LDL-Cho. Neither blood nor plasma viscosity was altered. Decrease of plasma cholesterol could be due to an increase of cholesterol and bile salts excretion leading to an increase of bile flow. In vitro experiments showed a direct interaction of PLc, at high concentration, with the erythrocyte membrane, inducing a switch from discocyte to stomatocyte. Only, PLc without hepatic metabolism produces hemorheological changes. Thus, PLc in vivo might be a pharmacological agent capable of decreasing plasma cholesterol.
Fil: Dominighini, Alicia. Universidad Nacional de Rosario. Facultad de Cs.medicas; Argentina
Fil: Ferrero, Mariana Cristina. Universidad Nacional de Rosario. Facultad de Cs.medicas; Argentina
Fil: Crossetti, D.. Universidad Nacional de Rosario. Facultad de Cs.medicas; Argentina
Fil: Ronco, Maria Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Fisiología Experimental (i); Argentina
Fil: Gonzalves, Jose. Universidad Nacional de Rosario. Facultad de Cs.medicas; Argentina
Fil: Urli, Leda. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Cs.fisiologicas; Argentina
Fil: Wagner, Marcelo Luis. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Gurni, Alberto Angel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Carnovale, Cristina Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Fisiología Experimental (i); Argentina
Fil: Luquita, Alejandra Nora. Universidad Nacional de Rosario. Facultad de Cs.medicas; Argentina
description It was demonstrated that Ligaria cuneifolia (Lc) crude extract increased blood viscosity and decreased plasma cholesterol in rats. In the present study, we analyzed the Lc proanthocyanidin enriched fraction (PLc) to determine if it is capable of altering the hemorheological parameters while diminishing the plasma cholesterol. In vivo studies in adult male Wistar rats, randomized in three groups (n = 6 each one) were performed: 1. Control: saline intraperitoneal (i.p.); 2. PLc 0.6 mg/100 g body weight (b.w.) i.p. and 3. PLc 3 mg/100 g b.w. i.p., every 24 hours during 3 days. In vitro studies: with blood obtained by cardiac puncture, separated in aliquots and incubated with: 1. Saline solution (Control); 2. PLc 0.1 mg/mL, and 3. PLc 1.0 mg/mL, equivalent to doses in vivo experiments. The results demonstrated that in vivo PLc 0.6 and PLc 3 reduced plasma cholesterol (Cho) and LDL-Cho. Neither blood nor plasma viscosity was altered. Decrease of plasma cholesterol could be due to an increase of cholesterol and bile salts excretion leading to an increase of bile flow. In vitro experiments showed a direct interaction of PLc, at high concentration, with the erythrocyte membrane, inducing a switch from discocyte to stomatocyte. Only, PLc without hepatic metabolism produces hemorheological changes. Thus, PLc in vivo might be a pharmacological agent capable of decreasing plasma cholesterol.
publishDate 2014
dc.date.none.fl_str_mv 2014-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6102
Dominighini, Alicia; Ferrero, Mariana Cristina; Crossetti, D.; Ronco, Maria Teresa; Gonzalves, Jose; et al.; Effects of proanthocyanidin enriched extract from Ligaria cuneifolia on plasma cholesterol and hemorheological parameters. In vivo and In vitro studies; Ios Press; Clinical Hemorheology And Microcirculation; 60; 3; 8-2014; 317-325
1386-0291
url http://hdl.handle.net/11336/6102
identifier_str_mv Dominighini, Alicia; Ferrero, Mariana Cristina; Crossetti, D.; Ronco, Maria Teresa; Gonzalves, Jose; et al.; Effects of proanthocyanidin enriched extract from Ligaria cuneifolia on plasma cholesterol and hemorheological parameters. In vivo and In vitro studies; Ios Press; Clinical Hemorheology And Microcirculation; 60; 3; 8-2014; 317-325
1386-0291
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://content.iospress.com/articles/clinical-hemorheology-and-microcirculation/ch1870
info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.3233/CH-141870
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Ios Press
publisher.none.fl_str_mv Ios Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781899635163136
score 13.229304

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