Detection of novel Betapapillomaviruses and Gammapapillomaviruses in eyebrow hair follicles using a singletube ‘hanging droplet’ PCR assay with modified pan-PV CODEHOP primers

Autores
Chouhy, Diego; Kocjan, Boštjan J.; Staheli, Jeannette P.; Bolatti, Elisa Maria; Hošnjak, Lea; Sagadin, Martin; Giri, Adriana Angelica; Rose, Timothy M.; Poljak, Mario
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A modified pan-PV consensus-degenerate hybrid oligonucleotide primer (CODEHOP) PCR was developed for generic and sensitive detection of a broad-spectrum of human papillomaviruses (HPVs) infecting the cutaneous epithelium. To test the analytical sensitivity of the assay we examined 149 eyebrow hair follicle specimens from immunocompetent male patients. HPV DNA was detected in 60% (89/149) of analysed eyebrow samples with a total of 48 different HPV sequences, representing 21 previously described HPVs and 27 putative novel HPV types. Evidence for ten novel HPV subtypes and seven viral variants, clustering to three out of five genera containing cutaneous HPVs, was also obtained. Thus, we have shown that the modified pan-PV CODEHOP PCR assay is able to identify multiple HPV types, even from different genera, in the same clinical sample. Overall, these results demonstrate that the pan-PV CODEHOP PCR is an excellent tool for screening and identification of novel cutaneous HPVs, even in samples with low viral loads.
Fil: Chouhy, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Kocjan, Boštjan J.. University of Ljubljana; Eslovenia
Fil: Staheli, Jeannette P.. Seattle Children’s Research Institute; Estados Unidos
Fil: Bolatti, Elisa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Hošnjak, Lea. University of Ljubljana; Eslovenia
Fil: Sagadin, Martin. University of Ljubljana; Eslovenia
Fil: Giri, Adriana Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Rose, Timothy M.. Seattle Children’s Research Institute; Estados Unidos
Fil: Poljak, Mario. University of Ljubljana; Eslovenia
Materia
CODEHOP
HPV
HUMAN PAPILLOMAVIRUS
MULTIPLE INFECTIONS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/88156
Acceder
id CONICETDig_f690a7941332a5a4b7af3d72be588f44
oai_identifier_str oai:ri.conicet.gov.ar:11336/88156
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Detection of novel Betapapillomaviruses and Gammapapillomaviruses in eyebrow hair follicles using a singletube ‘hanging droplet’ PCR assay with modified pan-PV CODEHOP primersChouhy, DiegoKocjan, Boštjan J.Staheli, Jeannette P.Bolatti, Elisa MariaHošnjak, LeaSagadin, MartinGiri, Adriana AngelicaRose, Timothy M.Poljak, MarioCODEHOPHPVHUMAN PAPILLOMAVIRUSMULTIPLE INFECTIONShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A modified pan-PV consensus-degenerate hybrid oligonucleotide primer (CODEHOP) PCR was developed for generic and sensitive detection of a broad-spectrum of human papillomaviruses (HPVs) infecting the cutaneous epithelium. To test the analytical sensitivity of the assay we examined 149 eyebrow hair follicle specimens from immunocompetent male patients. HPV DNA was detected in 60% (89/149) of analysed eyebrow samples with a total of 48 different HPV sequences, representing 21 previously described HPVs and 27 putative novel HPV types. Evidence for ten novel HPV subtypes and seven viral variants, clustering to three out of five genera containing cutaneous HPVs, was also obtained. Thus, we have shown that the modified pan-PV CODEHOP PCR assay is able to identify multiple HPV types, even from different genera, in the same clinical sample. Overall, these results demonstrate that the pan-PV CODEHOP PCR is an excellent tool for screening and identification of novel cutaneous HPVs, even in samples with low viral loads.Fil: Chouhy, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Kocjan, Boštjan J.. University of Ljubljana; EsloveniaFil: Staheli, Jeannette P.. Seattle Children’s Research Institute; Estados UnidosFil: Bolatti, Elisa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Hošnjak, Lea. University of Ljubljana; EsloveniaFil: Sagadin, Martin. University of Ljubljana; EsloveniaFil: Giri, Adriana Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Rose, Timothy M.. Seattle Children’s Research Institute; Estados UnidosFil: Poljak, Mario. University of Ljubljana; EsloveniaSociety for General Microbiology2018-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/88156Chouhy, Diego; Kocjan, Boštjan J.; Staheli, Jeannette P.; Bolatti, Elisa Maria; Hošnjak, Lea; et al.; Detection of novel Betapapillomaviruses and Gammapapillomaviruses in eyebrow hair follicles using a singletube ‘hanging droplet’ PCR assay with modified pan-PV CODEHOP primers; Society for General Microbiology; Journal of General Virology; 99; 1; 1-2018; 109-1180022-1317CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000988#tab2info:eu-repo/semantics/altIdentifier/doi/10.1099/jgv.0.000988info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:33Zoai:ri.conicet.gov.ar:11336/88156instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:34.203CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection of novel Betapapillomaviruses and Gammapapillomaviruses in eyebrow hair follicles using a singletube ‘hanging droplet’ PCR assay with modified pan-PV CODEHOP primers
title Detection of novel Betapapillomaviruses and Gammapapillomaviruses in eyebrow hair follicles using a singletube ‘hanging droplet’ PCR assay with modified pan-PV CODEHOP primers
spellingShingle Detection of novel Betapapillomaviruses and Gammapapillomaviruses in eyebrow hair follicles using a singletube ‘hanging droplet’ PCR assay with modified pan-PV CODEHOP primers
Chouhy, Diego
CODEHOP
HPV
HUMAN PAPILLOMAVIRUS
MULTIPLE INFECTIONS
title_short Detection of novel Betapapillomaviruses and Gammapapillomaviruses in eyebrow hair follicles using a singletube ‘hanging droplet’ PCR assay with modified pan-PV CODEHOP primers
title_full Detection of novel Betapapillomaviruses and Gammapapillomaviruses in eyebrow hair follicles using a singletube ‘hanging droplet’ PCR assay with modified pan-PV CODEHOP primers
title_fullStr Detection of novel Betapapillomaviruses and Gammapapillomaviruses in eyebrow hair follicles using a singletube ‘hanging droplet’ PCR assay with modified pan-PV CODEHOP primers
title_full_unstemmed Detection of novel Betapapillomaviruses and Gammapapillomaviruses in eyebrow hair follicles using a singletube ‘hanging droplet’ PCR assay with modified pan-PV CODEHOP primers
title_sort Detection of novel Betapapillomaviruses and Gammapapillomaviruses in eyebrow hair follicles using a singletube ‘hanging droplet’ PCR assay with modified pan-PV CODEHOP primers
dc.creator.none.fl_str_mv Chouhy, Diego
Kocjan, Boštjan J.
Staheli, Jeannette P.
Bolatti, Elisa Maria
Hošnjak, Lea
Sagadin, Martin
Giri, Adriana Angelica
Rose, Timothy M.
Poljak, Mario
author Chouhy, Diego
author_facet Chouhy, Diego
Kocjan, Boštjan J.
Staheli, Jeannette P.
Bolatti, Elisa Maria
Hošnjak, Lea
Sagadin, Martin
Giri, Adriana Angelica
Rose, Timothy M.
Poljak, Mario
author_role author
author2 Kocjan, Boštjan J.
Staheli, Jeannette P.
Bolatti, Elisa Maria
Hošnjak, Lea
Sagadin, Martin
Giri, Adriana Angelica
Rose, Timothy M.
Poljak, Mario
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CODEHOP
HPV
HUMAN PAPILLOMAVIRUS
MULTIPLE INFECTIONS
topic CODEHOP
HPV
HUMAN PAPILLOMAVIRUS
MULTIPLE INFECTIONS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A modified pan-PV consensus-degenerate hybrid oligonucleotide primer (CODEHOP) PCR was developed for generic and sensitive detection of a broad-spectrum of human papillomaviruses (HPVs) infecting the cutaneous epithelium. To test the analytical sensitivity of the assay we examined 149 eyebrow hair follicle specimens from immunocompetent male patients. HPV DNA was detected in 60% (89/149) of analysed eyebrow samples with a total of 48 different HPV sequences, representing 21 previously described HPVs and 27 putative novel HPV types. Evidence for ten novel HPV subtypes and seven viral variants, clustering to three out of five genera containing cutaneous HPVs, was also obtained. Thus, we have shown that the modified pan-PV CODEHOP PCR assay is able to identify multiple HPV types, even from different genera, in the same clinical sample. Overall, these results demonstrate that the pan-PV CODEHOP PCR is an excellent tool for screening and identification of novel cutaneous HPVs, even in samples with low viral loads.
Fil: Chouhy, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Kocjan, Boštjan J.. University of Ljubljana; Eslovenia
Fil: Staheli, Jeannette P.. Seattle Children’s Research Institute; Estados Unidos
Fil: Bolatti, Elisa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Hošnjak, Lea. University of Ljubljana; Eslovenia
Fil: Sagadin, Martin. University of Ljubljana; Eslovenia
Fil: Giri, Adriana Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Rose, Timothy M.. Seattle Children’s Research Institute; Estados Unidos
Fil: Poljak, Mario. University of Ljubljana; Eslovenia
description A modified pan-PV consensus-degenerate hybrid oligonucleotide primer (CODEHOP) PCR was developed for generic and sensitive detection of a broad-spectrum of human papillomaviruses (HPVs) infecting the cutaneous epithelium. To test the analytical sensitivity of the assay we examined 149 eyebrow hair follicle specimens from immunocompetent male patients. HPV DNA was detected in 60% (89/149) of analysed eyebrow samples with a total of 48 different HPV sequences, representing 21 previously described HPVs and 27 putative novel HPV types. Evidence for ten novel HPV subtypes and seven viral variants, clustering to three out of five genera containing cutaneous HPVs, was also obtained. Thus, we have shown that the modified pan-PV CODEHOP PCR assay is able to identify multiple HPV types, even from different genera, in the same clinical sample. Overall, these results demonstrate that the pan-PV CODEHOP PCR is an excellent tool for screening and identification of novel cutaneous HPVs, even in samples with low viral loads.
publishDate 2018
dc.date.none.fl_str_mv 2018-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/88156
Chouhy, Diego; Kocjan, Boštjan J.; Staheli, Jeannette P.; Bolatti, Elisa Maria; Hošnjak, Lea; et al.; Detection of novel Betapapillomaviruses and Gammapapillomaviruses in eyebrow hair follicles using a singletube ‘hanging droplet’ PCR assay with modified pan-PV CODEHOP primers; Society for General Microbiology; Journal of General Virology; 99; 1; 1-2018; 109-118
0022-1317
CONICET Digital
CONICET
url http://hdl.handle.net/11336/88156
identifier_str_mv Chouhy, Diego; Kocjan, Boštjan J.; Staheli, Jeannette P.; Bolatti, Elisa Maria; Hošnjak, Lea; et al.; Detection of novel Betapapillomaviruses and Gammapapillomaviruses in eyebrow hair follicles using a singletube ‘hanging droplet’ PCR assay with modified pan-PV CODEHOP primers; Society for General Microbiology; Journal of General Virology; 99; 1; 1-2018; 109-118
0022-1317
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000988#tab2
info:eu-repo/semantics/altIdentifier/doi/10.1099/jgv.0.000988
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for General Microbiology
publisher.none.fl_str_mv Society for General Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270124912934912
score 13.13397

Publicaciones similares