Propolis feeding modifies the expression of immune genes in challenged honey bees by injection with Paenibacillus larvae

Autores
Dominguez, Enzo; Quintana, Silvina; Medici, Sandra Karina; Moliné, Maria de la Paz; Moran Giardini, Paloma; Gende, Liesel Brenda; Damiani, Natalia
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Bees do not seem to consume propolis under natural conditions, despite having a similar composition of polyphenols to that of pollen and nectar. These compounds have been shown to be active against bee pathogens such as the bacteria Paenibacillus larvae and the mite Varroa destructor, and to stimulate the expression of immune genes in worker bees when they are added to the nest. Overexpression of genes related to detoxification and reduction of fat bodies have also been observed in bees under forced ingestion. The gene expression of Toll18Wheeler, abaecin, hymenoptecin, vitellogenin (Vg), and domeless was determined using RT-qPCR, with the beta-actin gene as control, in bees fed for 10 days on a sugar and protein diet with (+P) and without (-P) propolis soft extract, and then subjected them to different immune treatments: Control (C), cutaneous injection (S), and injection of P. larvae (PL). The results showed that honey bees fed with propolis consumed significantly less food than control bees during the 10-day feeding (p=0.037). Besides, both feeding and treatments affected the relative expression of all studied genes, and there was an interaction between the main factors. Bees in the +P/PL and +P/S groups showed lower relative expression of abaecin and hymenoptecin than bees in the -P/PL (abaecin: p=3.36e-06; hymenoptecin: p=0.002) and -P/S (abaecin: p=1.78e-13; hymenoptecin: p=3.45e-13) groups, respectively. Moreover, feeding with propolis modified the expression of the Vg gene in response to bacterial injection. These results suggest that supplementation with propolis influences the regulation of immune system pathways in immune-challenged bees. This study contributes to answering the question of whether bees would benefit from consuming propolis under natural conditions.
Fil: Dominguez, Enzo. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Quintana, Silvina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Medici, Sandra Karina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Moliné, Maria de la Paz. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; Argentina
Fil: Moran Giardini, Paloma. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Gende, Liesel Brenda. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Damiani, Natalia. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
48th International Apicultural Congress: Sustainable Beekeeping, from the south of the world
Santiago
Chile
Federación Internacional de Asociaciones de Apicultura
Materia
APIS MELLIFERA
PROPOLIS
PAENIBACILLUS LARVAE
FEED
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/267072
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Propolis feeding modifies the expression of immune genes in challenged honey bees by injection with Paenibacillus larvaeDominguez, EnzoQuintana, SilvinaMedici, Sandra KarinaMoliné, Maria de la PazMoran Giardini, PalomaGende, Liesel BrendaDamiani, NataliaAPIS MELLIFERAPROPOLISPAENIBACILLUS LARVAEFEEDhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Bees do not seem to consume propolis under natural conditions, despite having a similar composition of polyphenols to that of pollen and nectar. These compounds have been shown to be active against bee pathogens such as the bacteria Paenibacillus larvae and the mite Varroa destructor, and to stimulate the expression of immune genes in worker bees when they are added to the nest. Overexpression of genes related to detoxification and reduction of fat bodies have also been observed in bees under forced ingestion. The gene expression of Toll18Wheeler, abaecin, hymenoptecin, vitellogenin (Vg), and domeless was determined using RT-qPCR, with the beta-actin gene as control, in bees fed for 10 days on a sugar and protein diet with (+P) and without (-P) propolis soft extract, and then subjected them to different immune treatments: Control (C), cutaneous injection (S), and injection of P. larvae (PL). The results showed that honey bees fed with propolis consumed significantly less food than control bees during the 10-day feeding (p=0.037). Besides, both feeding and treatments affected the relative expression of all studied genes, and there was an interaction between the main factors. Bees in the +P/PL and +P/S groups showed lower relative expression of abaecin and hymenoptecin than bees in the -P/PL (abaecin: p=3.36e-06; hymenoptecin: p=0.002) and -P/S (abaecin: p=1.78e-13; hymenoptecin: p=3.45e-13) groups, respectively. Moreover, feeding with propolis modified the expression of the Vg gene in response to bacterial injection. These results suggest that supplementation with propolis influences the regulation of immune system pathways in immune-challenged bees. This study contributes to answering the question of whether bees would benefit from consuming propolis under natural conditions.Fil: Dominguez, Enzo. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Quintana, Silvina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Medici, Sandra Karina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Moliné, Maria de la Paz. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; ArgentinaFil: Moran Giardini, Paloma. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Gende, Liesel Brenda. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Damiani, Natalia. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina48th International Apicultural Congress: Sustainable Beekeeping, from the south of the worldSantiagoChileFederación Internacional de Asociaciones de ApiculturaFederación Internacional de Asociaciones de Apicultura2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/267072Propolis feeding modifies the expression of immune genes in challenged honey bees by injection with Paenibacillus larvae; 48th International Apicultural Congress: Sustainable Beekeeping, from the south of the world; Santiago; Chile; 2023; 1-342CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://apimondia2023.com/docs/abstract-book.pdfinfo:eu-repo/semantics/altIdentifier/url/https://zenodo.org/records/14633103Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:41Zoai:ri.conicet.gov.ar:11336/267072instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:41.799CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Propolis feeding modifies the expression of immune genes in challenged honey bees by injection with Paenibacillus larvae
title Propolis feeding modifies the expression of immune genes in challenged honey bees by injection with Paenibacillus larvae
spellingShingle Propolis feeding modifies the expression of immune genes in challenged honey bees by injection with Paenibacillus larvae
Dominguez, Enzo
APIS MELLIFERA
PROPOLIS
PAENIBACILLUS LARVAE
FEED
title_short Propolis feeding modifies the expression of immune genes in challenged honey bees by injection with Paenibacillus larvae
title_full Propolis feeding modifies the expression of immune genes in challenged honey bees by injection with Paenibacillus larvae
title_fullStr Propolis feeding modifies the expression of immune genes in challenged honey bees by injection with Paenibacillus larvae
title_full_unstemmed Propolis feeding modifies the expression of immune genes in challenged honey bees by injection with Paenibacillus larvae
title_sort Propolis feeding modifies the expression of immune genes in challenged honey bees by injection with Paenibacillus larvae
dc.creator.none.fl_str_mv Dominguez, Enzo
Quintana, Silvina
Medici, Sandra Karina
Moliné, Maria de la Paz
Moran Giardini, Paloma
Gende, Liesel Brenda
Damiani, Natalia
author Dominguez, Enzo
author_facet Dominguez, Enzo
Quintana, Silvina
Medici, Sandra Karina
Moliné, Maria de la Paz
Moran Giardini, Paloma
Gende, Liesel Brenda
Damiani, Natalia
author_role author
author2 Quintana, Silvina
Medici, Sandra Karina
Moliné, Maria de la Paz
Moran Giardini, Paloma
Gende, Liesel Brenda
Damiani, Natalia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv APIS MELLIFERA
PROPOLIS
PAENIBACILLUS LARVAE
FEED
topic APIS MELLIFERA
PROPOLIS
PAENIBACILLUS LARVAE
FEED
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bees do not seem to consume propolis under natural conditions, despite having a similar composition of polyphenols to that of pollen and nectar. These compounds have been shown to be active against bee pathogens such as the bacteria Paenibacillus larvae and the mite Varroa destructor, and to stimulate the expression of immune genes in worker bees when they are added to the nest. Overexpression of genes related to detoxification and reduction of fat bodies have also been observed in bees under forced ingestion. The gene expression of Toll18Wheeler, abaecin, hymenoptecin, vitellogenin (Vg), and domeless was determined using RT-qPCR, with the beta-actin gene as control, in bees fed for 10 days on a sugar and protein diet with (+P) and without (-P) propolis soft extract, and then subjected them to different immune treatments: Control (C), cutaneous injection (S), and injection of P. larvae (PL). The results showed that honey bees fed with propolis consumed significantly less food than control bees during the 10-day feeding (p=0.037). Besides, both feeding and treatments affected the relative expression of all studied genes, and there was an interaction between the main factors. Bees in the +P/PL and +P/S groups showed lower relative expression of abaecin and hymenoptecin than bees in the -P/PL (abaecin: p=3.36e-06; hymenoptecin: p=0.002) and -P/S (abaecin: p=1.78e-13; hymenoptecin: p=3.45e-13) groups, respectively. Moreover, feeding with propolis modified the expression of the Vg gene in response to bacterial injection. These results suggest that supplementation with propolis influences the regulation of immune system pathways in immune-challenged bees. This study contributes to answering the question of whether bees would benefit from consuming propolis under natural conditions.
Fil: Dominguez, Enzo. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Quintana, Silvina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Medici, Sandra Karina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Moliné, Maria de la Paz. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; Argentina
Fil: Moran Giardini, Paloma. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Gende, Liesel Brenda. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Damiani, Natalia. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
48th International Apicultural Congress: Sustainable Beekeeping, from the south of the world
Santiago
Chile
Federación Internacional de Asociaciones de Apicultura
description Bees do not seem to consume propolis under natural conditions, despite having a similar composition of polyphenols to that of pollen and nectar. These compounds have been shown to be active against bee pathogens such as the bacteria Paenibacillus larvae and the mite Varroa destructor, and to stimulate the expression of immune genes in worker bees when they are added to the nest. Overexpression of genes related to detoxification and reduction of fat bodies have also been observed in bees under forced ingestion. The gene expression of Toll18Wheeler, abaecin, hymenoptecin, vitellogenin (Vg), and domeless was determined using RT-qPCR, with the beta-actin gene as control, in bees fed for 10 days on a sugar and protein diet with (+P) and without (-P) propolis soft extract, and then subjected them to different immune treatments: Control (C), cutaneous injection (S), and injection of P. larvae (PL). The results showed that honey bees fed with propolis consumed significantly less food than control bees during the 10-day feeding (p=0.037). Besides, both feeding and treatments affected the relative expression of all studied genes, and there was an interaction between the main factors. Bees in the +P/PL and +P/S groups showed lower relative expression of abaecin and hymenoptecin than bees in the -P/PL (abaecin: p=3.36e-06; hymenoptecin: p=0.002) and -P/S (abaecin: p=1.78e-13; hymenoptecin: p=3.45e-13) groups, respectively. Moreover, feeding with propolis modified the expression of the Vg gene in response to bacterial injection. These results suggest that supplementation with propolis influences the regulation of immune system pathways in immune-challenged bees. This study contributes to answering the question of whether bees would benefit from consuming propolis under natural conditions.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/267072
Propolis feeding modifies the expression of immune genes in challenged honey bees by injection with Paenibacillus larvae; 48th International Apicultural Congress: Sustainable Beekeeping, from the south of the world; Santiago; Chile; 2023; 1-342
CONICET Digital
CONICET
url http://hdl.handle.net/11336/267072
identifier_str_mv Propolis feeding modifies the expression of immune genes in challenged honey bees by injection with Paenibacillus larvae; 48th International Apicultural Congress: Sustainable Beekeeping, from the south of the world; Santiago; Chile; 2023; 1-342
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Federación Internacional de Asociaciones de Apicultura
publisher.none.fl_str_mv Federación Internacional de Asociaciones de Apicultura
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