Acute Hypotensive, Diuretic and Antioxidant Activities Induced by Urtica circularis

Autores
Rodriguez Basso, A.; Marrassini, Carla; Anesini, Claudia Alejandra; Gorzalczany, Susana Beatriz
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: The aim of this study was to investigate the possible hypotensive and diuretic effects of ethanolic extract of Urtica circularis (Hicken) Sorarú (Urticaceae) using preclinical methods. Place and Duration of Study: Department of Pharmacology, School of Pharmacy and Biochemistry, Universidad de Buenos Aires, from July 2015 to January 2016. Methodology: Effect on blood pressure and heart rate on anaesthetized normotensive and hypertensive rats were measured using Statham Gould P23ID pressure transducer coupled to a Grass 79D polygraph. Rats were placed in metabolic cages in order to collect urine. Urinary volume was measured and sodium and potassium concentration was estimated from each urine sample using indirect ion-selective electrode potentiometry. The vasorelaxant activity of major compound was studied using isolated aortic rings. Antioxidant activity was estimated measuring 2,2 diphenyl 2 picryl hydrazyl hydrate radical scavenging activity. Results: The intravenous administration of the extract of U. circularis (0.1–30 mg/kg) in anaesthetized normotensive and hypertensive rats caused a dose-dependent reduction in the mean arterial pressure without affecting the heart rate. The greater reduction of blood pressure induced by U. circularis was observed in hypertensive rats (30 mg/kg: Spontaneously Hypertensive Rat: -34.7±3.3 mmHg, Spague Dawley: -18.3±3.9 mmHg). Cumulative urinary excretions 24 h after treatment with the extract 100 and 300 mg/kg were 18.2±1.2 and 14.9±1.5 mL respectively, significantly higher than the control group (9.0±1.3 mL). The addition of cumulative concentrations of vicenin-2 (10-7-10-4M) generated relaxation in endothelium-intact aortic rings pre-contracted with 10–7M Phenylephrine (Emax = 66.2±3.5%). Extract showed antioxidant activity reaching 45% of DPPH scavenging activity at 1000 μg/mL, meanwhile the flavonoid reached 20% of scavenger capacity. Conclusion: U. circularis, has a diuretic, antioxidant and hypotensive effect. Vicenin-2, the major component of this extract showed vasorelaxant activity, potentially responsible for the properties of the extract.
Fil: Rodriguez Basso, A.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; Argentina
Fil: Marrassini, Carla. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Anesini, Claudia Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gorzalczany, Susana Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; Argentina
Materia
Hypotensive
Diuretic
Vasorelaxant
Antioxidant
Urtica Circularis
Vicenin-2
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/40372
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/40372
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Acute Hypotensive, Diuretic and Antioxidant Activities Induced by Urtica circularisRodriguez Basso, A.Marrassini, CarlaAnesini, Claudia AlejandraGorzalczany, Susana BeatrizHypotensiveDiureticVasorelaxantAntioxidantUrtica CircularisVicenin-2https://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Aims: The aim of this study was to investigate the possible hypotensive and diuretic effects of ethanolic extract of Urtica circularis (Hicken) Sorarú (Urticaceae) using preclinical methods. Place and Duration of Study: Department of Pharmacology, School of Pharmacy and Biochemistry, Universidad de Buenos Aires, from July 2015 to January 2016. Methodology: Effect on blood pressure and heart rate on anaesthetized normotensive and hypertensive rats were measured using Statham Gould P23ID pressure transducer coupled to a Grass 79D polygraph. Rats were placed in metabolic cages in order to collect urine. Urinary volume was measured and sodium and potassium concentration was estimated from each urine sample using indirect ion-selective electrode potentiometry. The vasorelaxant activity of major compound was studied using isolated aortic rings. Antioxidant activity was estimated measuring 2,2 diphenyl 2 picryl hydrazyl hydrate radical scavenging activity. Results: The intravenous administration of the extract of U. circularis (0.1–30 mg/kg) in anaesthetized normotensive and hypertensive rats caused a dose-dependent reduction in the mean arterial pressure without affecting the heart rate. The greater reduction of blood pressure induced by U. circularis was observed in hypertensive rats (30 mg/kg: Spontaneously Hypertensive Rat: -34.7±3.3 mmHg, Spague Dawley: -18.3±3.9 mmHg). Cumulative urinary excretions 24 h after treatment with the extract 100 and 300 mg/kg were 18.2±1.2 and 14.9±1.5 mL respectively, significantly higher than the control group (9.0±1.3 mL). The addition of cumulative concentrations of vicenin-2 (10-7-10-4M) generated relaxation in endothelium-intact aortic rings pre-contracted with 10–7M Phenylephrine (Emax = 66.2±3.5%). Extract showed antioxidant activity reaching 45% of DPPH scavenging activity at 1000 μg/mL, meanwhile the flavonoid reached 20% of scavenger capacity. Conclusion: U. circularis, has a diuretic, antioxidant and hypotensive effect. Vicenin-2, the major component of this extract showed vasorelaxant activity, potentially responsible for the properties of the extract.Fil: Rodriguez Basso, A.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; ArgentinaFil: Marrassini, Carla. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Anesini, Claudia Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gorzalczany, Susana Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; ArgentinaScienceDomain International2016-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/40372Rodriguez Basso, A.; Marrassini, Carla; Anesini, Claudia Alejandra; Gorzalczany, Susana Beatriz; Acute Hypotensive, Diuretic and Antioxidant Activities Induced by Urtica circularis; ScienceDomain International; European Journal of Medicinal Plants; 14; 2; 1-2016; 1-92231-0894CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.9734/EJMP/2016/25661info:eu-repo/semantics/altIdentifier/url/http://sciencedomain.org/abstract/14180info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:42Zoai:ri.conicet.gov.ar:11336/40372instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:42.811CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Acute Hypotensive, Diuretic and Antioxidant Activities Induced by Urtica circularis
title Acute Hypotensive, Diuretic and Antioxidant Activities Induced by Urtica circularis
spellingShingle Acute Hypotensive, Diuretic and Antioxidant Activities Induced by Urtica circularis
Rodriguez Basso, A.
Hypotensive
Diuretic
Vasorelaxant
Antioxidant
Urtica Circularis
Vicenin-2
title_short Acute Hypotensive, Diuretic and Antioxidant Activities Induced by Urtica circularis
title_full Acute Hypotensive, Diuretic and Antioxidant Activities Induced by Urtica circularis
title_fullStr Acute Hypotensive, Diuretic and Antioxidant Activities Induced by Urtica circularis
title_full_unstemmed Acute Hypotensive, Diuretic and Antioxidant Activities Induced by Urtica circularis
title_sort Acute Hypotensive, Diuretic and Antioxidant Activities Induced by Urtica circularis
dc.creator.none.fl_str_mv Rodriguez Basso, A.
Marrassini, Carla
Anesini, Claudia Alejandra
Gorzalczany, Susana Beatriz
author Rodriguez Basso, A.
author_facet Rodriguez Basso, A.
Marrassini, Carla
Anesini, Claudia Alejandra
Gorzalczany, Susana Beatriz
author_role author
author2 Marrassini, Carla
Anesini, Claudia Alejandra
Gorzalczany, Susana Beatriz
author2_role author
author
author
dc.subject.none.fl_str_mv Hypotensive
Diuretic
Vasorelaxant
Antioxidant
Urtica Circularis
Vicenin-2
topic Hypotensive
Diuretic
Vasorelaxant
Antioxidant
Urtica Circularis
Vicenin-2
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Aims: The aim of this study was to investigate the possible hypotensive and diuretic effects of ethanolic extract of Urtica circularis (Hicken) Sorarú (Urticaceae) using preclinical methods. Place and Duration of Study: Department of Pharmacology, School of Pharmacy and Biochemistry, Universidad de Buenos Aires, from July 2015 to January 2016. Methodology: Effect on blood pressure and heart rate on anaesthetized normotensive and hypertensive rats were measured using Statham Gould P23ID pressure transducer coupled to a Grass 79D polygraph. Rats were placed in metabolic cages in order to collect urine. Urinary volume was measured and sodium and potassium concentration was estimated from each urine sample using indirect ion-selective electrode potentiometry. The vasorelaxant activity of major compound was studied using isolated aortic rings. Antioxidant activity was estimated measuring 2,2 diphenyl 2 picryl hydrazyl hydrate radical scavenging activity. Results: The intravenous administration of the extract of U. circularis (0.1–30 mg/kg) in anaesthetized normotensive and hypertensive rats caused a dose-dependent reduction in the mean arterial pressure without affecting the heart rate. The greater reduction of blood pressure induced by U. circularis was observed in hypertensive rats (30 mg/kg: Spontaneously Hypertensive Rat: -34.7±3.3 mmHg, Spague Dawley: -18.3±3.9 mmHg). Cumulative urinary excretions 24 h after treatment with the extract 100 and 300 mg/kg were 18.2±1.2 and 14.9±1.5 mL respectively, significantly higher than the control group (9.0±1.3 mL). The addition of cumulative concentrations of vicenin-2 (10-7-10-4M) generated relaxation in endothelium-intact aortic rings pre-contracted with 10–7M Phenylephrine (Emax = 66.2±3.5%). Extract showed antioxidant activity reaching 45% of DPPH scavenging activity at 1000 μg/mL, meanwhile the flavonoid reached 20% of scavenger capacity. Conclusion: U. circularis, has a diuretic, antioxidant and hypotensive effect. Vicenin-2, the major component of this extract showed vasorelaxant activity, potentially responsible for the properties of the extract.
Fil: Rodriguez Basso, A.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; Argentina
Fil: Marrassini, Carla. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Anesini, Claudia Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gorzalczany, Susana Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología; Argentina
description Aims: The aim of this study was to investigate the possible hypotensive and diuretic effects of ethanolic extract of Urtica circularis (Hicken) Sorarú (Urticaceae) using preclinical methods. Place and Duration of Study: Department of Pharmacology, School of Pharmacy and Biochemistry, Universidad de Buenos Aires, from July 2015 to January 2016. Methodology: Effect on blood pressure and heart rate on anaesthetized normotensive and hypertensive rats were measured using Statham Gould P23ID pressure transducer coupled to a Grass 79D polygraph. Rats were placed in metabolic cages in order to collect urine. Urinary volume was measured and sodium and potassium concentration was estimated from each urine sample using indirect ion-selective electrode potentiometry. The vasorelaxant activity of major compound was studied using isolated aortic rings. Antioxidant activity was estimated measuring 2,2 diphenyl 2 picryl hydrazyl hydrate radical scavenging activity. Results: The intravenous administration of the extract of U. circularis (0.1–30 mg/kg) in anaesthetized normotensive and hypertensive rats caused a dose-dependent reduction in the mean arterial pressure without affecting the heart rate. The greater reduction of blood pressure induced by U. circularis was observed in hypertensive rats (30 mg/kg: Spontaneously Hypertensive Rat: -34.7±3.3 mmHg, Spague Dawley: -18.3±3.9 mmHg). Cumulative urinary excretions 24 h after treatment with the extract 100 and 300 mg/kg were 18.2±1.2 and 14.9±1.5 mL respectively, significantly higher than the control group (9.0±1.3 mL). The addition of cumulative concentrations of vicenin-2 (10-7-10-4M) generated relaxation in endothelium-intact aortic rings pre-contracted with 10–7M Phenylephrine (Emax = 66.2±3.5%). Extract showed antioxidant activity reaching 45% of DPPH scavenging activity at 1000 μg/mL, meanwhile the flavonoid reached 20% of scavenger capacity. Conclusion: U. circularis, has a diuretic, antioxidant and hypotensive effect. Vicenin-2, the major component of this extract showed vasorelaxant activity, potentially responsible for the properties of the extract.
publishDate 2016
dc.date.none.fl_str_mv 2016-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/40372
Rodriguez Basso, A.; Marrassini, Carla; Anesini, Claudia Alejandra; Gorzalczany, Susana Beatriz; Acute Hypotensive, Diuretic and Antioxidant Activities Induced by Urtica circularis; ScienceDomain International; European Journal of Medicinal Plants; 14; 2; 1-2016; 1-9
2231-0894
CONICET Digital
CONICET
url http://hdl.handle.net/11336/40372
identifier_str_mv Rodriguez Basso, A.; Marrassini, Carla; Anesini, Claudia Alejandra; Gorzalczany, Susana Beatriz; Acute Hypotensive, Diuretic and Antioxidant Activities Induced by Urtica circularis; ScienceDomain International; European Journal of Medicinal Plants; 14; 2; 1-2016; 1-9
2231-0894
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.9734/EJMP/2016/25661
info:eu-repo/semantics/altIdentifier/url/http://sciencedomain.org/abstract/14180
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv ScienceDomain International
publisher.none.fl_str_mv ScienceDomain International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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