Layer-to-layer distance determines the performance of 3D bio-electrochemical lamellar anodes in microbial energy transduction processes

Autores
Massazza, Diego Ariel; Busalmen, Juan Pablo; Parra, Rodrigo; Romeo, Hernan Esteban
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Microbial fuel cells (MFCs) harness the metabolic machinery of electro-active bacteria to transfer electrons from organic molecules to polarized anodes. In this context, increasingly higher anode surface areas have been pursued for maximizing MFC performance. In this study we prepared 3D layered Ti4O7 electrodes with different interlayer spacings (from 10 to 100 μm) but maintaining the same total void fraction (90%), so as to modify the electrode surface-to-volume ratios. This allowed us to test the hypothesis that there must be a limit in surface area per unit volume restricting the efficiency of 3D porous bio-electrochemical anodes. The lamellar scaffolds were evaluated in three-electrode cells cultured with G. sulfurreducens. Regardless of the electrode interlayer spacing or the biofilm developmental stage, the electron transfer rate was constant (0.11 pA per bacterium), with current scaling linearly with the size of the microbial population. However, maximum volumetric current densities (20 ± 0.8 kA m-3) were not obtained from electrodes with maximum surface-to-volume ratios (shorter interlayer distances), because bacterial biomass was not directly proportional to the surface area. This demonstrated that, by controlling the spacing between layers, it is possible to modulate the amount of bacteria per electrode unit volume, this ratio determining the final electrode performance. The limit obtained in surface area suggested that other effects, such as fluid dynamic constraints inside the "slit-shaped" pores, must be playing a critical role in anode performance.
Fil: Massazza, Diego Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Busalmen, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Parra, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Romeo, Hernan Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Materia
Biofilms
Electrodos porosos
Sistemas bioelectroquímicos
bio-electrochemical
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/81645
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/81645
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Layer-to-layer distance determines the performance of 3D bio-electrochemical lamellar anodes in microbial energy transduction processesMassazza, Diego ArielBusalmen, Juan PabloParra, RodrigoRomeo, Hernan EstebanBiofilmsElectrodos porososSistemas bioelectroquímicosbio-electrochemicalhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2Microbial fuel cells (MFCs) harness the metabolic machinery of electro-active bacteria to transfer electrons from organic molecules to polarized anodes. In this context, increasingly higher anode surface areas have been pursued for maximizing MFC performance. In this study we prepared 3D layered Ti4O7 electrodes with different interlayer spacings (from 10 to 100 μm) but maintaining the same total void fraction (90%), so as to modify the electrode surface-to-volume ratios. This allowed us to test the hypothesis that there must be a limit in surface area per unit volume restricting the efficiency of 3D porous bio-electrochemical anodes. The lamellar scaffolds were evaluated in three-electrode cells cultured with G. sulfurreducens. Regardless of the electrode interlayer spacing or the biofilm developmental stage, the electron transfer rate was constant (0.11 pA per bacterium), with current scaling linearly with the size of the microbial population. However, maximum volumetric current densities (20 ± 0.8 kA m-3) were not obtained from electrodes with maximum surface-to-volume ratios (shorter interlayer distances), because bacterial biomass was not directly proportional to the surface area. This demonstrated that, by controlling the spacing between layers, it is possible to modulate the amount of bacteria per electrode unit volume, this ratio determining the final electrode performance. The limit obtained in surface area suggested that other effects, such as fluid dynamic constraints inside the "slit-shaped" pores, must be playing a critical role in anode performance.Fil: Massazza, Diego Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Busalmen, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Parra, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Romeo, Hernan Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaRoyal Society of Chemistry2018-04-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/81645Massazza, Diego Ariel; Busalmen, Juan Pablo; Parra, Rodrigo; Romeo, Hernan Esteban; Layer-to-layer distance determines the performance of 3D bio-electrochemical lamellar anodes in microbial energy transduction processes; Royal Society of Chemistry; Journal of Materials Chemistry A; 6; 21; 24-4-2018; 10019-100272050-74882050-7496CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2018/TA/C8TA02793Einfo:eu-repo/semantics/altIdentifier/doi/10.1039/C8TA02793Einfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:55Zoai:ri.conicet.gov.ar:11336/81645instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:55.955CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Layer-to-layer distance determines the performance of 3D bio-electrochemical lamellar anodes in microbial energy transduction processes
title Layer-to-layer distance determines the performance of 3D bio-electrochemical lamellar anodes in microbial energy transduction processes
spellingShingle Layer-to-layer distance determines the performance of 3D bio-electrochemical lamellar anodes in microbial energy transduction processes
Massazza, Diego Ariel
Biofilms
Electrodos porosos
Sistemas bioelectroquímicos
bio-electrochemical
title_short Layer-to-layer distance determines the performance of 3D bio-electrochemical lamellar anodes in microbial energy transduction processes
title_full Layer-to-layer distance determines the performance of 3D bio-electrochemical lamellar anodes in microbial energy transduction processes
title_fullStr Layer-to-layer distance determines the performance of 3D bio-electrochemical lamellar anodes in microbial energy transduction processes
title_full_unstemmed Layer-to-layer distance determines the performance of 3D bio-electrochemical lamellar anodes in microbial energy transduction processes
title_sort Layer-to-layer distance determines the performance of 3D bio-electrochemical lamellar anodes in microbial energy transduction processes
dc.creator.none.fl_str_mv Massazza, Diego Ariel
Busalmen, Juan Pablo
Parra, Rodrigo
Romeo, Hernan Esteban
author Massazza, Diego Ariel
author_facet Massazza, Diego Ariel
Busalmen, Juan Pablo
Parra, Rodrigo
Romeo, Hernan Esteban
author_role author
author2 Busalmen, Juan Pablo
Parra, Rodrigo
Romeo, Hernan Esteban
author2_role author
author
author
dc.subject.none.fl_str_mv Biofilms
Electrodos porosos
Sistemas bioelectroquímicos
bio-electrochemical
topic Biofilms
Electrodos porosos
Sistemas bioelectroquímicos
bio-electrochemical
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Microbial fuel cells (MFCs) harness the metabolic machinery of electro-active bacteria to transfer electrons from organic molecules to polarized anodes. In this context, increasingly higher anode surface areas have been pursued for maximizing MFC performance. In this study we prepared 3D layered Ti4O7 electrodes with different interlayer spacings (from 10 to 100 μm) but maintaining the same total void fraction (90%), so as to modify the electrode surface-to-volume ratios. This allowed us to test the hypothesis that there must be a limit in surface area per unit volume restricting the efficiency of 3D porous bio-electrochemical anodes. The lamellar scaffolds were evaluated in three-electrode cells cultured with G. sulfurreducens. Regardless of the electrode interlayer spacing or the biofilm developmental stage, the electron transfer rate was constant (0.11 pA per bacterium), with current scaling linearly with the size of the microbial population. However, maximum volumetric current densities (20 ± 0.8 kA m-3) were not obtained from electrodes with maximum surface-to-volume ratios (shorter interlayer distances), because bacterial biomass was not directly proportional to the surface area. This demonstrated that, by controlling the spacing between layers, it is possible to modulate the amount of bacteria per electrode unit volume, this ratio determining the final electrode performance. The limit obtained in surface area suggested that other effects, such as fluid dynamic constraints inside the "slit-shaped" pores, must be playing a critical role in anode performance.
Fil: Massazza, Diego Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Busalmen, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Parra, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Romeo, Hernan Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
description Microbial fuel cells (MFCs) harness the metabolic machinery of electro-active bacteria to transfer electrons from organic molecules to polarized anodes. In this context, increasingly higher anode surface areas have been pursued for maximizing MFC performance. In this study we prepared 3D layered Ti4O7 electrodes with different interlayer spacings (from 10 to 100 μm) but maintaining the same total void fraction (90%), so as to modify the electrode surface-to-volume ratios. This allowed us to test the hypothesis that there must be a limit in surface area per unit volume restricting the efficiency of 3D porous bio-electrochemical anodes. The lamellar scaffolds were evaluated in three-electrode cells cultured with G. sulfurreducens. Regardless of the electrode interlayer spacing or the biofilm developmental stage, the electron transfer rate was constant (0.11 pA per bacterium), with current scaling linearly with the size of the microbial population. However, maximum volumetric current densities (20 ± 0.8 kA m-3) were not obtained from electrodes with maximum surface-to-volume ratios (shorter interlayer distances), because bacterial biomass was not directly proportional to the surface area. This demonstrated that, by controlling the spacing between layers, it is possible to modulate the amount of bacteria per electrode unit volume, this ratio determining the final electrode performance. The limit obtained in surface area suggested that other effects, such as fluid dynamic constraints inside the "slit-shaped" pores, must be playing a critical role in anode performance.
publishDate 2018
dc.date.none.fl_str_mv 2018-04-24
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/81645
Massazza, Diego Ariel; Busalmen, Juan Pablo; Parra, Rodrigo; Romeo, Hernan Esteban; Layer-to-layer distance determines the performance of 3D bio-electrochemical lamellar anodes in microbial energy transduction processes; Royal Society of Chemistry; Journal of Materials Chemistry A; 6; 21; 24-4-2018; 10019-10027
2050-7488
2050-7496
CONICET Digital
CONICET
url http://hdl.handle.net/11336/81645
identifier_str_mv Massazza, Diego Ariel; Busalmen, Juan Pablo; Parra, Rodrigo; Romeo, Hernan Esteban; Layer-to-layer distance determines the performance of 3D bio-electrochemical lamellar anodes in microbial energy transduction processes; Royal Society of Chemistry; Journal of Materials Chemistry A; 6; 21; 24-4-2018; 10019-10027
2050-7488
2050-7496
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2018/TA/C8TA02793E
info:eu-repo/semantics/altIdentifier/doi/10.1039/C8TA02793E
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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