Intestinal toxicity of deoxynivalenol is limited by Lactobacillus rhamnosus RC007 in pig jejunum explants

Autores
García, Gisela Romina; Payros, Delphine; Pinton, Philippe; Dogi, Cecilia Ana; Laffitte, Joëlle; Neves, Manon; Gonzalez Pereyra, Maria Laura; Cavaglieri, Lilia Reneé; Oswald, Isabelle P.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Probiotics have been explored to stimulate gut health in weaned pigs, when they started to consume solid diet where mycotoxins could be present. The aim of this study was to evaluate the effect of Lactobacillus rhamnosus RC007 on the intestinal toxicity of deoxynivalenol (DON) in an ex vivo model. Jejunal explants, obtained from 5-week-old crossbred castrated male piglets, were kept as control, exposed for 3 h to 10 μM DON, incubated for 4 h with 109 CFU/mL L. rhamnosus, or pre-incubated 1 h with 109L. rhamnosus and exposed to DON. Histological lesions were observed, para- and transcellular intestinal permeability was measured in Ussing chambers. The expression levels of mRNA encoding six inflammatory cytokines (CCL20, IL-10, IL-1β, TNFα, IL-8 and IL-22) were determined by RT-PCR. The expressions of the phosphorylated MAP kinases p42/p44 and p38 were assessed by immunoblotting. Exposure to DON induced histological changes, significantly increased the expression of CCL20, IL-1β, TNFα, IL-8, IL-22 and IL-10, increased the intestinal paracellular permeability and activated MAP kinases. Incubation with L. rhamnosus alone did not have any significant effect. By contrast, the pre-incubation with L. rhamnosus reduced all the effects of DON: the histological alterations, the pro-inflammatory response, the paracellular permeability and the phosphorylation of MAP kinases. Of note, L. rhamnosus did not adsorb DON and only slightly degrade the toxin. In conclusion, L. rhamnosus RC007 is a promising probiotic which, included as feed additive, can decrease the intestinal toxicity of DON.
Fil: García, Gisela Romina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Payros, Delphine. Instituto National de Recherches Agronomiques. Centre de Recherches de Toulouse; Francia. Université Paul Sabatier; Francia
Fil: Pinton, Philippe. Instituto National de Recherches Agronomiques. Centre de Recherches de Toulouse; Francia. Université Paul Sabatier; Francia
Fil: Dogi, Cecilia Ana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Laffitte, Joëlle. Instituto National de Recherches Agronomiques. Centre de Recherches de Toulouse; Francia. Université Paul Sabatier; Francia
Fil: Neves, Manon. Instituto National de Recherches Agronomiques. Centre de Recherches de Toulouse; Francia. Université Paul Sabatier; Francia
Fil: Gonzalez Pereyra, Maria Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Oswald, Isabelle P.. Instituto National de Recherches Agronomiques. Centre de Recherches de Toulouse; Francia. Université Paul Sabatier; Francia
Materia
DEOXYNIVALENOL
INTESTINE
LACTOBACILLUS RHAMNOSUS
MYCOTOXIN
TOXICITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/133026
Acceder
id CONICETDig_f478d0f8e5c9992859a8ae9a4ecd93a9
oai_identifier_str oai:ri.conicet.gov.ar:11336/133026
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Intestinal toxicity of deoxynivalenol is limited by Lactobacillus rhamnosus RC007 in pig jejunum explantsGarcía, Gisela RominaPayros, DelphinePinton, PhilippeDogi, Cecilia AnaLaffitte, JoëlleNeves, ManonGonzalez Pereyra, Maria LauraCavaglieri, Lilia ReneéOswald, Isabelle P.DEOXYNIVALENOLINTESTINELACTOBACILLUS RHAMNOSUSMYCOTOXINTOXICITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Probiotics have been explored to stimulate gut health in weaned pigs, when they started to consume solid diet where mycotoxins could be present. The aim of this study was to evaluate the effect of Lactobacillus rhamnosus RC007 on the intestinal toxicity of deoxynivalenol (DON) in an ex vivo model. Jejunal explants, obtained from 5-week-old crossbred castrated male piglets, were kept as control, exposed for 3 h to 10 μM DON, incubated for 4 h with 109 CFU/mL L. rhamnosus, or pre-incubated 1 h with 109L. rhamnosus and exposed to DON. Histological lesions were observed, para- and transcellular intestinal permeability was measured in Ussing chambers. The expression levels of mRNA encoding six inflammatory cytokines (CCL20, IL-10, IL-1β, TNFα, IL-8 and IL-22) were determined by RT-PCR. The expressions of the phosphorylated MAP kinases p42/p44 and p38 were assessed by immunoblotting. Exposure to DON induced histological changes, significantly increased the expression of CCL20, IL-1β, TNFα, IL-8, IL-22 and IL-10, increased the intestinal paracellular permeability and activated MAP kinases. Incubation with L. rhamnosus alone did not have any significant effect. By contrast, the pre-incubation with L. rhamnosus reduced all the effects of DON: the histological alterations, the pro-inflammatory response, the paracellular permeability and the phosphorylation of MAP kinases. Of note, L. rhamnosus did not adsorb DON and only slightly degrade the toxin. In conclusion, L. rhamnosus RC007 is a promising probiotic which, included as feed additive, can decrease the intestinal toxicity of DON.Fil: García, Gisela Romina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Payros, Delphine. Instituto National de Recherches Agronomiques. Centre de Recherches de Toulouse; Francia. Université Paul Sabatier; FranciaFil: Pinton, Philippe. Instituto National de Recherches Agronomiques. Centre de Recherches de Toulouse; Francia. Université Paul Sabatier; FranciaFil: Dogi, Cecilia Ana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Laffitte, Joëlle. Instituto National de Recherches Agronomiques. Centre de Recherches de Toulouse; Francia. Université Paul Sabatier; FranciaFil: Neves, Manon. Instituto National de Recherches Agronomiques. Centre de Recherches de Toulouse; Francia. Université Paul Sabatier; FranciaFil: Gonzalez Pereyra, Maria Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Oswald, Isabelle P.. Instituto National de Recherches Agronomiques. Centre de Recherches de Toulouse; Francia. Université Paul Sabatier; FranciaSpringer2018-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/133026García, Gisela Romina; Payros, Delphine; Pinton, Philippe; Dogi, Cecilia Ana; Laffitte, Joëlle; et al.; Intestinal toxicity of deoxynivalenol is limited by Lactobacillus rhamnosus RC007 in pig jejunum explants; Springer; Archives of Toxicology; 92; 2; 2-2018; 983-9930340-5761CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00204-017-2083-xinfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00204-017-2083-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T12:19:19Zoai:ri.conicet.gov.ar:11336/133026instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 12:19:19.743CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Intestinal toxicity of deoxynivalenol is limited by Lactobacillus rhamnosus RC007 in pig jejunum explants
title Intestinal toxicity of deoxynivalenol is limited by Lactobacillus rhamnosus RC007 in pig jejunum explants
spellingShingle Intestinal toxicity of deoxynivalenol is limited by Lactobacillus rhamnosus RC007 in pig jejunum explants
García, Gisela Romina
DEOXYNIVALENOL
INTESTINE
LACTOBACILLUS RHAMNOSUS
MYCOTOXIN
TOXICITY
title_short Intestinal toxicity of deoxynivalenol is limited by Lactobacillus rhamnosus RC007 in pig jejunum explants
title_full Intestinal toxicity of deoxynivalenol is limited by Lactobacillus rhamnosus RC007 in pig jejunum explants
title_fullStr Intestinal toxicity of deoxynivalenol is limited by Lactobacillus rhamnosus RC007 in pig jejunum explants
title_full_unstemmed Intestinal toxicity of deoxynivalenol is limited by Lactobacillus rhamnosus RC007 in pig jejunum explants
title_sort Intestinal toxicity of deoxynivalenol is limited by Lactobacillus rhamnosus RC007 in pig jejunum explants
dc.creator.none.fl_str_mv García, Gisela Romina
Payros, Delphine
Pinton, Philippe
Dogi, Cecilia Ana
Laffitte, Joëlle
Neves, Manon
Gonzalez Pereyra, Maria Laura
Cavaglieri, Lilia Reneé
Oswald, Isabelle P.
author García, Gisela Romina
author_facet García, Gisela Romina
Payros, Delphine
Pinton, Philippe
Dogi, Cecilia Ana
Laffitte, Joëlle
Neves, Manon
Gonzalez Pereyra, Maria Laura
Cavaglieri, Lilia Reneé
Oswald, Isabelle P.
author_role author
author2 Payros, Delphine
Pinton, Philippe
Dogi, Cecilia Ana
Laffitte, Joëlle
Neves, Manon
Gonzalez Pereyra, Maria Laura
Cavaglieri, Lilia Reneé
Oswald, Isabelle P.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DEOXYNIVALENOL
INTESTINE
LACTOBACILLUS RHAMNOSUS
MYCOTOXIN
TOXICITY
topic DEOXYNIVALENOL
INTESTINE
LACTOBACILLUS RHAMNOSUS
MYCOTOXIN
TOXICITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Probiotics have been explored to stimulate gut health in weaned pigs, when they started to consume solid diet where mycotoxins could be present. The aim of this study was to evaluate the effect of Lactobacillus rhamnosus RC007 on the intestinal toxicity of deoxynivalenol (DON) in an ex vivo model. Jejunal explants, obtained from 5-week-old crossbred castrated male piglets, were kept as control, exposed for 3 h to 10 μM DON, incubated for 4 h with 109 CFU/mL L. rhamnosus, or pre-incubated 1 h with 109L. rhamnosus and exposed to DON. Histological lesions were observed, para- and transcellular intestinal permeability was measured in Ussing chambers. The expression levels of mRNA encoding six inflammatory cytokines (CCL20, IL-10, IL-1β, TNFα, IL-8 and IL-22) were determined by RT-PCR. The expressions of the phosphorylated MAP kinases p42/p44 and p38 were assessed by immunoblotting. Exposure to DON induced histological changes, significantly increased the expression of CCL20, IL-1β, TNFα, IL-8, IL-22 and IL-10, increased the intestinal paracellular permeability and activated MAP kinases. Incubation with L. rhamnosus alone did not have any significant effect. By contrast, the pre-incubation with L. rhamnosus reduced all the effects of DON: the histological alterations, the pro-inflammatory response, the paracellular permeability and the phosphorylation of MAP kinases. Of note, L. rhamnosus did not adsorb DON and only slightly degrade the toxin. In conclusion, L. rhamnosus RC007 is a promising probiotic which, included as feed additive, can decrease the intestinal toxicity of DON.
Fil: García, Gisela Romina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Payros, Delphine. Instituto National de Recherches Agronomiques. Centre de Recherches de Toulouse; Francia. Université Paul Sabatier; Francia
Fil: Pinton, Philippe. Instituto National de Recherches Agronomiques. Centre de Recherches de Toulouse; Francia. Université Paul Sabatier; Francia
Fil: Dogi, Cecilia Ana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Laffitte, Joëlle. Instituto National de Recherches Agronomiques. Centre de Recherches de Toulouse; Francia. Université Paul Sabatier; Francia
Fil: Neves, Manon. Instituto National de Recherches Agronomiques. Centre de Recherches de Toulouse; Francia. Université Paul Sabatier; Francia
Fil: Gonzalez Pereyra, Maria Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Oswald, Isabelle P.. Instituto National de Recherches Agronomiques. Centre de Recherches de Toulouse; Francia. Université Paul Sabatier; Francia
description Probiotics have been explored to stimulate gut health in weaned pigs, when they started to consume solid diet where mycotoxins could be present. The aim of this study was to evaluate the effect of Lactobacillus rhamnosus RC007 on the intestinal toxicity of deoxynivalenol (DON) in an ex vivo model. Jejunal explants, obtained from 5-week-old crossbred castrated male piglets, were kept as control, exposed for 3 h to 10 μM DON, incubated for 4 h with 109 CFU/mL L. rhamnosus, or pre-incubated 1 h with 109L. rhamnosus and exposed to DON. Histological lesions were observed, para- and transcellular intestinal permeability was measured in Ussing chambers. The expression levels of mRNA encoding six inflammatory cytokines (CCL20, IL-10, IL-1β, TNFα, IL-8 and IL-22) were determined by RT-PCR. The expressions of the phosphorylated MAP kinases p42/p44 and p38 were assessed by immunoblotting. Exposure to DON induced histological changes, significantly increased the expression of CCL20, IL-1β, TNFα, IL-8, IL-22 and IL-10, increased the intestinal paracellular permeability and activated MAP kinases. Incubation with L. rhamnosus alone did not have any significant effect. By contrast, the pre-incubation with L. rhamnosus reduced all the effects of DON: the histological alterations, the pro-inflammatory response, the paracellular permeability and the phosphorylation of MAP kinases. Of note, L. rhamnosus did not adsorb DON and only slightly degrade the toxin. In conclusion, L. rhamnosus RC007 is a promising probiotic which, included as feed additive, can decrease the intestinal toxicity of DON.
publishDate 2018
dc.date.none.fl_str_mv 2018-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/133026
García, Gisela Romina; Payros, Delphine; Pinton, Philippe; Dogi, Cecilia Ana; Laffitte, Joëlle; et al.; Intestinal toxicity of deoxynivalenol is limited by Lactobacillus rhamnosus RC007 in pig jejunum explants; Springer; Archives of Toxicology; 92; 2; 2-2018; 983-993
0340-5761
CONICET Digital
CONICET
url http://hdl.handle.net/11336/133026
identifier_str_mv García, Gisela Romina; Payros, Delphine; Pinton, Philippe; Dogi, Cecilia Ana; Laffitte, Joëlle; et al.; Intestinal toxicity of deoxynivalenol is limited by Lactobacillus rhamnosus RC007 in pig jejunum explants; Springer; Archives of Toxicology; 92; 2; 2-2018; 983-993
0340-5761
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1007/s00204-017-2083-x
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dc.format.none.fl_str_mv application/pdf
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dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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