Textile-dye polluted waters as a source for selecting chromate-reducing yeasts through Cr(VI)-enriched microcosms

Autores
Fernandez, Pablo Marcelo; Cabral, Maria Eugenia; Delgado, Osvaldo Daniel; Fariña, Julia Inés; Castellanos de Figueroa, Lucia Ines
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chromate-resistant microorganisms able to reduce toxic Cr(VI) into insoluble Cr(III) are seen as prom- ising candidates for alleviating Cr(VI)-contamination. In this study, chromate-reducing yeasts could be isolated from a textile-dye effluent and associated biofilm by using microcosm methodology with pe- riodical 1 mM Cr(VI)-pulses. Viable cell count seemed to reveal a progressive tolerance increase to Cr(VI). However, fungal colony numbers decreased after 108 h of cultivation most likely as a consequence of the accumulated toxic effects of metal during enrichment. From 49 different Cr(VI)-tolerant fungal mor- photypes isolated under selective conditions, 12 yeasts showed resistance up to 50 mM and 6 fila- mentous fungi up to 2 mM. These highly tolerant yeasts could be subsequently grouped into 8 OTUs (Operational Taxonomic Units) according to the ITS1-NL4 RFLP (Restriction Fragment Length Polymor- phism) analysis. From them, microsatellite amplification, sequencing and Cr(VI)-removal ability allowed to select two representative isolates. A polyphasic approach including morphological, physiological/ biochemical characterization and molecular taxonomy analysis allowed to identify these isolates as Cyberlindnera jadinii M9 (previously Pichia jadinii) and Wickerhamomyces anomalus M10 (previously Pichia anomala). Cy. jadinii M9 and W. anomalus M10 were grown in YNB’ medium plus 1 mM Cr(VI) at 25 C and pH 5.0, causing complete chromium removal before reaching 48 h of cultivation. Flame Atomic Absorption Spectroscopy (FAAS) assays suggested that Cr(VI) withdrawal was coupled to Cr(III) appearance. Electron microscopy studies indicated absence of precipitates on the cell wall region or microprecipitates into the cellular cytoplasm. These complementary results revealed that biospeciation to Cr(III) would the main detoxification mechanism in selected chromate-resistant yeasts, which could be thus envisaged as promising tools for future biological treatment of Cr(VI) pollution.
Fil: Fernandez, Pablo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Cabral, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Castellanos de Figueroa, Lucia Ines. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Materia
BIOREMEDIATION
BIOSPECIATION
CR(VI)
CR(VI)-RESISTANT YEASTS
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acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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CONICET Digital (CONICET)
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Consejo Nacional de Investigaciones Científicas y Técnicas
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oai:ri.conicet.gov.ar:11336/1710
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/1710
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Textile-dye polluted waters as a source for selecting chromate-reducing yeasts through Cr(VI)-enriched microcosmsFernandez, Pablo MarceloCabral, Maria EugeniaDelgado, Osvaldo DanielFariña, Julia InésCastellanos de Figueroa, Lucia InesBIOREMEDIATIONBIOSPECIATIONCR(VI)CR(VI)-RESISTANT YEASTShttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Chromate-resistant microorganisms able to reduce toxic Cr(VI) into insoluble Cr(III) are seen as prom- ising candidates for alleviating Cr(VI)-contamination. In this study, chromate-reducing yeasts could be isolated from a textile-dye effluent and associated biofilm by using microcosm methodology with pe- riodical 1 mM Cr(VI)-pulses. Viable cell count seemed to reveal a progressive tolerance increase to Cr(VI). However, fungal colony numbers decreased after 108 h of cultivation most likely as a consequence of the accumulated toxic effects of metal during enrichment. From 49 different Cr(VI)-tolerant fungal mor- photypes isolated under selective conditions, 12 yeasts showed resistance up to 50 mM and 6 fila- mentous fungi up to 2 mM. These highly tolerant yeasts could be subsequently grouped into 8 OTUs (Operational Taxonomic Units) according to the ITS1-NL4 RFLP (Restriction Fragment Length Polymor- phism) analysis. From them, microsatellite amplification, sequencing and Cr(VI)-removal ability allowed to select two representative isolates. A polyphasic approach including morphological, physiological/ biochemical characterization and molecular taxonomy analysis allowed to identify these isolates as Cyberlindnera jadinii M9 (previously Pichia jadinii) and Wickerhamomyces anomalus M10 (previously Pichia anomala). Cy. jadinii M9 and W. anomalus M10 were grown in YNB’ medium plus 1 mM Cr(VI) at 25 C and pH 5.0, causing complete chromium removal before reaching 48 h of cultivation. Flame Atomic Absorption Spectroscopy (FAAS) assays suggested that Cr(VI) withdrawal was coupled to Cr(III) appearance. Electron microscopy studies indicated absence of precipitates on the cell wall region or microprecipitates into the cellular cytoplasm. These complementary results revealed that biospeciation to Cr(III) would the main detoxification mechanism in selected chromate-resistant yeasts, which could be thus envisaged as promising tools for future biological treatment of Cr(VI) pollution.Fil: Fernandez, Pablo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Cabral, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Fariña, Julia Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Castellanos de Figueroa, Lucia Ines. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaElsevier2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1710Fernandez, Pablo Marcelo; Cabral, Maria Eugenia; Delgado, Osvaldo Daniel; Fariña, Julia Inés; Castellanos de Figueroa, Lucia Ines; Textile-dye polluted waters as a source for selecting chromate-reducing yeasts through Cr(VI)-enriched microcosms; Elsevier; International Biodeterioration and Biodegradation; 79; 1-2013; 28-350964-8305enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2013.01.009info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0964830513000115info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:14Zoai:ri.conicet.gov.ar:11336/1710instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:14.916CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Textile-dye polluted waters as a source for selecting chromate-reducing yeasts through Cr(VI)-enriched microcosms
title Textile-dye polluted waters as a source for selecting chromate-reducing yeasts through Cr(VI)-enriched microcosms
spellingShingle Textile-dye polluted waters as a source for selecting chromate-reducing yeasts through Cr(VI)-enriched microcosms
Fernandez, Pablo Marcelo
BIOREMEDIATION
BIOSPECIATION
CR(VI)
CR(VI)-RESISTANT YEASTS
title_short Textile-dye polluted waters as a source for selecting chromate-reducing yeasts through Cr(VI)-enriched microcosms
title_full Textile-dye polluted waters as a source for selecting chromate-reducing yeasts through Cr(VI)-enriched microcosms
title_fullStr Textile-dye polluted waters as a source for selecting chromate-reducing yeasts through Cr(VI)-enriched microcosms
title_full_unstemmed Textile-dye polluted waters as a source for selecting chromate-reducing yeasts through Cr(VI)-enriched microcosms
title_sort Textile-dye polluted waters as a source for selecting chromate-reducing yeasts through Cr(VI)-enriched microcosms
dc.creator.none.fl_str_mv Fernandez, Pablo Marcelo
Cabral, Maria Eugenia
Delgado, Osvaldo Daniel
Fariña, Julia Inés
Castellanos de Figueroa, Lucia Ines
author Fernandez, Pablo Marcelo
author_facet Fernandez, Pablo Marcelo
Cabral, Maria Eugenia
Delgado, Osvaldo Daniel
Fariña, Julia Inés
Castellanos de Figueroa, Lucia Ines
author_role author
author2 Cabral, Maria Eugenia
Delgado, Osvaldo Daniel
Fariña, Julia Inés
Castellanos de Figueroa, Lucia Ines
author2_role author
author
author
author
dc.subject.none.fl_str_mv BIOREMEDIATION
BIOSPECIATION
CR(VI)
CR(VI)-RESISTANT YEASTS
topic BIOREMEDIATION
BIOSPECIATION
CR(VI)
CR(VI)-RESISTANT YEASTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Chromate-resistant microorganisms able to reduce toxic Cr(VI) into insoluble Cr(III) are seen as prom- ising candidates for alleviating Cr(VI)-contamination. In this study, chromate-reducing yeasts could be isolated from a textile-dye effluent and associated biofilm by using microcosm methodology with pe- riodical 1 mM Cr(VI)-pulses. Viable cell count seemed to reveal a progressive tolerance increase to Cr(VI). However, fungal colony numbers decreased after 108 h of cultivation most likely as a consequence of the accumulated toxic effects of metal during enrichment. From 49 different Cr(VI)-tolerant fungal mor- photypes isolated under selective conditions, 12 yeasts showed resistance up to 50 mM and 6 fila- mentous fungi up to 2 mM. These highly tolerant yeasts could be subsequently grouped into 8 OTUs (Operational Taxonomic Units) according to the ITS1-NL4 RFLP (Restriction Fragment Length Polymor- phism) analysis. From them, microsatellite amplification, sequencing and Cr(VI)-removal ability allowed to select two representative isolates. A polyphasic approach including morphological, physiological/ biochemical characterization and molecular taxonomy analysis allowed to identify these isolates as Cyberlindnera jadinii M9 (previously Pichia jadinii) and Wickerhamomyces anomalus M10 (previously Pichia anomala). Cy. jadinii M9 and W. anomalus M10 were grown in YNB’ medium plus 1 mM Cr(VI) at 25 C and pH 5.0, causing complete chromium removal before reaching 48 h of cultivation. Flame Atomic Absorption Spectroscopy (FAAS) assays suggested that Cr(VI) withdrawal was coupled to Cr(III) appearance. Electron microscopy studies indicated absence of precipitates on the cell wall region or microprecipitates into the cellular cytoplasm. These complementary results revealed that biospeciation to Cr(III) would the main detoxification mechanism in selected chromate-resistant yeasts, which could be thus envisaged as promising tools for future biological treatment of Cr(VI) pollution.
Fil: Fernandez, Pablo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Cabral, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Castellanos de Figueroa, Lucia Ines. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
description Chromate-resistant microorganisms able to reduce toxic Cr(VI) into insoluble Cr(III) are seen as prom- ising candidates for alleviating Cr(VI)-contamination. In this study, chromate-reducing yeasts could be isolated from a textile-dye effluent and associated biofilm by using microcosm methodology with pe- riodical 1 mM Cr(VI)-pulses. Viable cell count seemed to reveal a progressive tolerance increase to Cr(VI). However, fungal colony numbers decreased after 108 h of cultivation most likely as a consequence of the accumulated toxic effects of metal during enrichment. From 49 different Cr(VI)-tolerant fungal mor- photypes isolated under selective conditions, 12 yeasts showed resistance up to 50 mM and 6 fila- mentous fungi up to 2 mM. These highly tolerant yeasts could be subsequently grouped into 8 OTUs (Operational Taxonomic Units) according to the ITS1-NL4 RFLP (Restriction Fragment Length Polymor- phism) analysis. From them, microsatellite amplification, sequencing and Cr(VI)-removal ability allowed to select two representative isolates. A polyphasic approach including morphological, physiological/ biochemical characterization and molecular taxonomy analysis allowed to identify these isolates as Cyberlindnera jadinii M9 (previously Pichia jadinii) and Wickerhamomyces anomalus M10 (previously Pichia anomala). Cy. jadinii M9 and W. anomalus M10 were grown in YNB’ medium plus 1 mM Cr(VI) at 25 C and pH 5.0, causing complete chromium removal before reaching 48 h of cultivation. Flame Atomic Absorption Spectroscopy (FAAS) assays suggested that Cr(VI) withdrawal was coupled to Cr(III) appearance. Electron microscopy studies indicated absence of precipitates on the cell wall region or microprecipitates into the cellular cytoplasm. These complementary results revealed that biospeciation to Cr(III) would the main detoxification mechanism in selected chromate-resistant yeasts, which could be thus envisaged as promising tools for future biological treatment of Cr(VI) pollution.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/1710
Fernandez, Pablo Marcelo; Cabral, Maria Eugenia; Delgado, Osvaldo Daniel; Fariña, Julia Inés; Castellanos de Figueroa, Lucia Ines; Textile-dye polluted waters as a source for selecting chromate-reducing yeasts through Cr(VI)-enriched microcosms; Elsevier; International Biodeterioration and Biodegradation; 79; 1-2013; 28-35
0964-8305
url http://hdl.handle.net/11336/1710
identifier_str_mv Fernandez, Pablo Marcelo; Cabral, Maria Eugenia; Delgado, Osvaldo Daniel; Fariña, Julia Inés; Castellanos de Figueroa, Lucia Ines; Textile-dye polluted waters as a source for selecting chromate-reducing yeasts through Cr(VI)-enriched microcosms; Elsevier; International Biodeterioration and Biodegradation; 79; 1-2013; 28-35
0964-8305
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2013.01.009
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0964830513000115
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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