Cr(VI) reduction by cell-free extracts of Pichia jadinii and Pichia anomala isolated from textile-dye factory effluents

Autores
Martorell, María Martha; Fernandez, Pablo Marcelo; Fariña, Julia Ines; Castellanos, Lucia Ines
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chromium-resistant yeasts isolated from contaminated environments can be used to reduce toxic Cr(VI). This study assessed in vitro reduction of hexavalent chromium using crude chromate reductase (CChR) of Pichia jadinii M9 and Pichia anomala M10, two yeasts isolated from a textile-dye factory effluent. CChRs were characterized based on optimal temperature, pH, use of electron donors, metal ions and initial Cr(VI) concentration in the reaction mixture. Both CChRs showed an increase in Cr(VI) reductase activity with addition of NAD(P)H as electron donor and were highly inhibited by Hg 2+ and Mn 2+. The CChR from P. jadinii M9 showed the highest chromate reductase activity at 60 °C and pH 6.0 in the presence of Cu 2+ or Na +, while P. anomala M10 CChR had the maximum activity at 50 °C and pH 7.0 in presence of Cu 2+. Initial Cr(VI) concentrations of 1.3 and 1.7 mM for CChRs of P. jadinii M9 and P. anomala M10 respectively were inhibitory.
Fil: Martorell, María Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fernandez, Pablo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Materia
BIOREMEDIATION
CHROMATE REDUCTASE
CR(VI)-REMOVAL
PICHIA SP
YEASTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/202088
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/202088
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cr(VI) reduction by cell-free extracts of Pichia jadinii and Pichia anomala isolated from textile-dye factory effluentsMartorell, María MarthaFernandez, Pablo MarceloFariña, Julia InesCastellanos, Lucia InesBIOREMEDIATIONCHROMATE REDUCTASECR(VI)-REMOVALPICHIA SPYEASTShttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Chromium-resistant yeasts isolated from contaminated environments can be used to reduce toxic Cr(VI). This study assessed in vitro reduction of hexavalent chromium using crude chromate reductase (CChR) of Pichia jadinii M9 and Pichia anomala M10, two yeasts isolated from a textile-dye factory effluent. CChRs were characterized based on optimal temperature, pH, use of electron donors, metal ions and initial Cr(VI) concentration in the reaction mixture. Both CChRs showed an increase in Cr(VI) reductase activity with addition of NAD(P)H as electron donor and were highly inhibited by Hg 2+ and Mn 2+. The CChR from P. jadinii M9 showed the highest chromate reductase activity at 60 °C and pH 6.0 in the presence of Cu 2+ or Na +, while P. anomala M10 CChR had the maximum activity at 50 °C and pH 7.0 in presence of Cu 2+. Initial Cr(VI) concentrations of 1.3 and 1.7 mM for CChRs of P. jadinii M9 and P. anomala M10 respectively were inhibitory.Fil: Martorell, María Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Fernandez, Pablo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaElsevier2012-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/202088Martorell, María Martha; Fernandez, Pablo Marcelo; Fariña, Julia Ines; Castellanos, Lucia Ines; Cr(VI) reduction by cell-free extracts of Pichia jadinii and Pichia anomala isolated from textile-dye factory effluents; Elsevier; International Biodeterioration and Biodegradation; 71; 7-2012; 80-850964-8305CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0964830512000935info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2012.04.007info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:39Zoai:ri.conicet.gov.ar:11336/202088instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:39.983CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cr(VI) reduction by cell-free extracts of Pichia jadinii and Pichia anomala isolated from textile-dye factory effluents
title Cr(VI) reduction by cell-free extracts of Pichia jadinii and Pichia anomala isolated from textile-dye factory effluents
spellingShingle Cr(VI) reduction by cell-free extracts of Pichia jadinii and Pichia anomala isolated from textile-dye factory effluents
Martorell, María Martha
BIOREMEDIATION
CHROMATE REDUCTASE
CR(VI)-REMOVAL
PICHIA SP
YEASTS
title_short Cr(VI) reduction by cell-free extracts of Pichia jadinii and Pichia anomala isolated from textile-dye factory effluents
title_full Cr(VI) reduction by cell-free extracts of Pichia jadinii and Pichia anomala isolated from textile-dye factory effluents
title_fullStr Cr(VI) reduction by cell-free extracts of Pichia jadinii and Pichia anomala isolated from textile-dye factory effluents
title_full_unstemmed Cr(VI) reduction by cell-free extracts of Pichia jadinii and Pichia anomala isolated from textile-dye factory effluents
title_sort Cr(VI) reduction by cell-free extracts of Pichia jadinii and Pichia anomala isolated from textile-dye factory effluents
dc.creator.none.fl_str_mv Martorell, María Martha
Fernandez, Pablo Marcelo
Fariña, Julia Ines
Castellanos, Lucia Ines
author Martorell, María Martha
author_facet Martorell, María Martha
Fernandez, Pablo Marcelo
Fariña, Julia Ines
Castellanos, Lucia Ines
author_role author
author2 Fernandez, Pablo Marcelo
Fariña, Julia Ines
Castellanos, Lucia Ines
author2_role author
author
author
dc.subject.none.fl_str_mv BIOREMEDIATION
CHROMATE REDUCTASE
CR(VI)-REMOVAL
PICHIA SP
YEASTS
topic BIOREMEDIATION
CHROMATE REDUCTASE
CR(VI)-REMOVAL
PICHIA SP
YEASTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Chromium-resistant yeasts isolated from contaminated environments can be used to reduce toxic Cr(VI). This study assessed in vitro reduction of hexavalent chromium using crude chromate reductase (CChR) of Pichia jadinii M9 and Pichia anomala M10, two yeasts isolated from a textile-dye factory effluent. CChRs were characterized based on optimal temperature, pH, use of electron donors, metal ions and initial Cr(VI) concentration in the reaction mixture. Both CChRs showed an increase in Cr(VI) reductase activity with addition of NAD(P)H as electron donor and were highly inhibited by Hg 2+ and Mn 2+. The CChR from P. jadinii M9 showed the highest chromate reductase activity at 60 °C and pH 6.0 in the presence of Cu 2+ or Na +, while P. anomala M10 CChR had the maximum activity at 50 °C and pH 7.0 in presence of Cu 2+. Initial Cr(VI) concentrations of 1.3 and 1.7 mM for CChRs of P. jadinii M9 and P. anomala M10 respectively were inhibitory.
Fil: Martorell, María Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fernandez, Pablo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
description Chromium-resistant yeasts isolated from contaminated environments can be used to reduce toxic Cr(VI). This study assessed in vitro reduction of hexavalent chromium using crude chromate reductase (CChR) of Pichia jadinii M9 and Pichia anomala M10, two yeasts isolated from a textile-dye factory effluent. CChRs were characterized based on optimal temperature, pH, use of electron donors, metal ions and initial Cr(VI) concentration in the reaction mixture. Both CChRs showed an increase in Cr(VI) reductase activity with addition of NAD(P)H as electron donor and were highly inhibited by Hg 2+ and Mn 2+. The CChR from P. jadinii M9 showed the highest chromate reductase activity at 60 °C and pH 6.0 in the presence of Cu 2+ or Na +, while P. anomala M10 CChR had the maximum activity at 50 °C and pH 7.0 in presence of Cu 2+. Initial Cr(VI) concentrations of 1.3 and 1.7 mM for CChRs of P. jadinii M9 and P. anomala M10 respectively were inhibitory.
publishDate 2012
dc.date.none.fl_str_mv 2012-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/202088
Martorell, María Martha; Fernandez, Pablo Marcelo; Fariña, Julia Ines; Castellanos, Lucia Ines; Cr(VI) reduction by cell-free extracts of Pichia jadinii and Pichia anomala isolated from textile-dye factory effluents; Elsevier; International Biodeterioration and Biodegradation; 71; 7-2012; 80-85
0964-8305
CONICET Digital
CONICET
url http://hdl.handle.net/11336/202088
identifier_str_mv Martorell, María Martha; Fernandez, Pablo Marcelo; Fariña, Julia Ines; Castellanos, Lucia Ines; Cr(VI) reduction by cell-free extracts of Pichia jadinii and Pichia anomala isolated from textile-dye factory effluents; Elsevier; International Biodeterioration and Biodegradation; 71; 7-2012; 80-85
0964-8305
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0964830512000935
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2012.04.007
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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