Down-regulation of NF-κB signaling by Gordonia bronchialis prevents the activation of gut epithelial cells

Autores
Smaldini, Paola Lorena; Stanford, John; Romanin, David Emmanuel; Fossati, Carlos Alberto; Docena, Guillermo H.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The immunomodulatory power of heat-killed Gordonia bronchialis was studied on gut epithelial cells activated with pro-inflammatory stimuli (flagellin, TNF-α or IL-1β). Light emission of luciferase-transfected epithelial cells and mRNA expression of IL-1β, TNF-α, IL-6, CCL20, IL-8 and MCP-1 were measured. NF-κB activation was assessed by immunofluorescence and immunoblotting, and induction of reactive oxygen species (ROS) was evaluated. In vivo inhibitory properties of G. bronchialis were studied with ligated intestinal loop assay and in a mouse model of food allergy. G. bronchialis promoted the down-regulation of the expression of CCL20 and IL-1β on activated epithelial cells in a dose-dependent manner. A concomitant blocking of nuclear p65 translocation with increased production of ROS was found. In vivo experiments confirmed the inhibition of CCL20 expression and the suppression of IgE sensitization and hypersensitivity symptoms in the food allergy mouse model. In conclusion, heat-killed G. bronchialis inhibited the activation of NF-κB pathway in human epithelial cells, and suppressed the expression of CCL20. These results indicate that G. bronchialis may be used to modulate the initial steps of innate immune activation, which further suppress the allergic sensitization. This approach may be exploited as a therapy for intestinal inflammation.
Fil: Smaldini, Paola Lorena. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Stanford, John. University College London; Estados Unidos
Fil: Romanin, David Emmanuel. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fossati, Carlos Alberto. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Docena, Guillermo H.. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Down-Regulation
Gordonia Bronchialis
Nf-Kb
Epithelial Cells
Ccl20
Inflammation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10848
Acceder
id CONICETDig_f44f5bd6b2be662fc11bc858bf03a69e
oai_identifier_str oai:ri.conicet.gov.ar:11336/10848
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Down-regulation of NF-κB signaling by Gordonia bronchialis prevents the activation of gut epithelial cellsSmaldini, Paola LorenaStanford, JohnRomanin, David EmmanuelFossati, Carlos AlbertoDocena, Guillermo H.Down-RegulationGordonia BronchialisNf-KbEpithelial CellsCcl20Inflammationhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The immunomodulatory power of heat-killed Gordonia bronchialis was studied on gut epithelial cells activated with pro-inflammatory stimuli (flagellin, TNF-α or IL-1β). Light emission of luciferase-transfected epithelial cells and mRNA expression of IL-1β, TNF-α, IL-6, CCL20, IL-8 and MCP-1 were measured. NF-κB activation was assessed by immunofluorescence and immunoblotting, and induction of reactive oxygen species (ROS) was evaluated. In vivo inhibitory properties of G. bronchialis were studied with ligated intestinal loop assay and in a mouse model of food allergy. G. bronchialis promoted the down-regulation of the expression of CCL20 and IL-1β on activated epithelial cells in a dose-dependent manner. A concomitant blocking of nuclear p65 translocation with increased production of ROS was found. In vivo experiments confirmed the inhibition of CCL20 expression and the suppression of IgE sensitization and hypersensitivity symptoms in the food allergy mouse model. In conclusion, heat-killed G. bronchialis inhibited the activation of NF-κB pathway in human epithelial cells, and suppressed the expression of CCL20. These results indicate that G. bronchialis may be used to modulate the initial steps of innate immune activation, which further suppress the allergic sensitization. This approach may be exploited as a therapy for intestinal inflammation.Fil: Smaldini, Paola Lorena. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Stanford, John. University College London; Estados UnidosFil: Romanin, David Emmanuel. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fossati, Carlos Alberto. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Docena, Guillermo H.. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSage Publications Ltd2014-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10848Smaldini, Paola Lorena; Stanford, John; Romanin, David Emmanuel; Fossati, Carlos Alberto; Docena, Guillermo H.; Down-regulation of NF-κB signaling by Gordonia bronchialis prevents the activation of gut epithelial cells; Sage Publications Ltd; Innate Immunity; 20; 6; 8-2014; 626-6381753-42591753-4267enginfo:eu-repo/semantics/altIdentifier/doi/10.1177/1753425913503577info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/abs/10.1177/1753425913503577info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:02:46Zoai:ri.conicet.gov.ar:11336/10848instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:02:46.634CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Down-regulation of NF-κB signaling by Gordonia bronchialis prevents the activation of gut epithelial cells
title Down-regulation of NF-κB signaling by Gordonia bronchialis prevents the activation of gut epithelial cells
spellingShingle Down-regulation of NF-κB signaling by Gordonia bronchialis prevents the activation of gut epithelial cells
Smaldini, Paola Lorena
Down-Regulation
Gordonia Bronchialis
Nf-Kb
Epithelial Cells
Ccl20
Inflammation
title_short Down-regulation of NF-κB signaling by Gordonia bronchialis prevents the activation of gut epithelial cells
title_full Down-regulation of NF-κB signaling by Gordonia bronchialis prevents the activation of gut epithelial cells
title_fullStr Down-regulation of NF-κB signaling by Gordonia bronchialis prevents the activation of gut epithelial cells
title_full_unstemmed Down-regulation of NF-κB signaling by Gordonia bronchialis prevents the activation of gut epithelial cells
title_sort Down-regulation of NF-κB signaling by Gordonia bronchialis prevents the activation of gut epithelial cells
dc.creator.none.fl_str_mv Smaldini, Paola Lorena
Stanford, John
Romanin, David Emmanuel
Fossati, Carlos Alberto
Docena, Guillermo H.
author Smaldini, Paola Lorena
author_facet Smaldini, Paola Lorena
Stanford, John
Romanin, David Emmanuel
Fossati, Carlos Alberto
Docena, Guillermo H.
author_role author
author2 Stanford, John
Romanin, David Emmanuel
Fossati, Carlos Alberto
Docena, Guillermo H.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Down-Regulation
Gordonia Bronchialis
Nf-Kb
Epithelial Cells
Ccl20
Inflammation
topic Down-Regulation
Gordonia Bronchialis
Nf-Kb
Epithelial Cells
Ccl20
Inflammation
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The immunomodulatory power of heat-killed Gordonia bronchialis was studied on gut epithelial cells activated with pro-inflammatory stimuli (flagellin, TNF-α or IL-1β). Light emission of luciferase-transfected epithelial cells and mRNA expression of IL-1β, TNF-α, IL-6, CCL20, IL-8 and MCP-1 were measured. NF-κB activation was assessed by immunofluorescence and immunoblotting, and induction of reactive oxygen species (ROS) was evaluated. In vivo inhibitory properties of G. bronchialis were studied with ligated intestinal loop assay and in a mouse model of food allergy. G. bronchialis promoted the down-regulation of the expression of CCL20 and IL-1β on activated epithelial cells in a dose-dependent manner. A concomitant blocking of nuclear p65 translocation with increased production of ROS was found. In vivo experiments confirmed the inhibition of CCL20 expression and the suppression of IgE sensitization and hypersensitivity symptoms in the food allergy mouse model. In conclusion, heat-killed G. bronchialis inhibited the activation of NF-κB pathway in human epithelial cells, and suppressed the expression of CCL20. These results indicate that G. bronchialis may be used to modulate the initial steps of innate immune activation, which further suppress the allergic sensitization. This approach may be exploited as a therapy for intestinal inflammation.
Fil: Smaldini, Paola Lorena. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Stanford, John. University College London; Estados Unidos
Fil: Romanin, David Emmanuel. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fossati, Carlos Alberto. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Docena, Guillermo H.. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The immunomodulatory power of heat-killed Gordonia bronchialis was studied on gut epithelial cells activated with pro-inflammatory stimuli (flagellin, TNF-α or IL-1β). Light emission of luciferase-transfected epithelial cells and mRNA expression of IL-1β, TNF-α, IL-6, CCL20, IL-8 and MCP-1 were measured. NF-κB activation was assessed by immunofluorescence and immunoblotting, and induction of reactive oxygen species (ROS) was evaluated. In vivo inhibitory properties of G. bronchialis were studied with ligated intestinal loop assay and in a mouse model of food allergy. G. bronchialis promoted the down-regulation of the expression of CCL20 and IL-1β on activated epithelial cells in a dose-dependent manner. A concomitant blocking of nuclear p65 translocation with increased production of ROS was found. In vivo experiments confirmed the inhibition of CCL20 expression and the suppression of IgE sensitization and hypersensitivity symptoms in the food allergy mouse model. In conclusion, heat-killed G. bronchialis inhibited the activation of NF-κB pathway in human epithelial cells, and suppressed the expression of CCL20. These results indicate that G. bronchialis may be used to modulate the initial steps of innate immune activation, which further suppress the allergic sensitization. This approach may be exploited as a therapy for intestinal inflammation.
publishDate 2014
dc.date.none.fl_str_mv 2014-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10848
Smaldini, Paola Lorena; Stanford, John; Romanin, David Emmanuel; Fossati, Carlos Alberto; Docena, Guillermo H.; Down-regulation of NF-κB signaling by Gordonia bronchialis prevents the activation of gut epithelial cells; Sage Publications Ltd; Innate Immunity; 20; 6; 8-2014; 626-638
1753-4259
1753-4267
url http://hdl.handle.net/11336/10848
identifier_str_mv Smaldini, Paola Lorena; Stanford, John; Romanin, David Emmanuel; Fossati, Carlos Alberto; Docena, Guillermo H.; Down-regulation of NF-κB signaling by Gordonia bronchialis prevents the activation of gut epithelial cells; Sage Publications Ltd; Innate Immunity; 20; 6; 8-2014; 626-638
1753-4259
1753-4267
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1177/1753425913503577
info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/abs/10.1177/1753425913503577
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sage Publications Ltd
publisher.none.fl_str_mv Sage Publications Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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