Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse mod...

Autores
Glaser, Viviane; de Paula Martins, Roberta; Hoffmann Vieira, Ana Julia; de Medeiros Oliveira, Eliana; Straliotto, Marcos Raniel; Mukdsi, Jorge Humberto; Torres, Alicia Ines; Fabro de Bem, Andreza; Farina, Marcelo; Teixeira da Rocha, Joao Batista; de Paul, Ana Lucia; Latini, Alexandra
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Interest in biochemistry of organoselenium compound has increased in the last decades, mainly due to their chemical and biological activities. Here, we investigated the protective effect of diphenyl diselenide (PhSe)2 (5 μmol/kg), in a mouse model of methylmercury (MeHg)-induced brain toxicity. Swiss male mice were divided into four experimental groups: control, (PhSe)2 (5 μmol/kg, subcutaneous administration), MeHg (40 mg/L, in tap water), and MeHg + (PhSe)2. After the treatment (21 days), the animals were killed and the cerebral cortex was analyzed. Electron microscopy indicated an enlarged and fused mitochondria leading to a reduced number of organelles, in the MeHg-exposed mice. Furthermore, cortical creatine kinase activity, a sensitive mitochondrial oxidative stress sensor, was almost abolished by MeHg. Subcutaneous (PhSe)2 co-treatment rescued from MeHg-induced mitochondrial alterations. (PhSe)2 also behaved as an enhancer of mitochondrial biogenesis, by increasing cortical mitochondria content in mouse-receiving (PhSe)2 alone. Mechanistically, (PhSe)2 (1 μM; 24 h) would trigger the cytoprotective Nrf-2 pathway for activating target genes, since astroglial cells exposed to the chalcogen showed increased content of hemeoxygenase type 1, a sensitive marker of the activation of this via. Thus, it is proposed that the (PhSe)2-neuroprotective effect might be linked to its mitoprotective activity.
Fil: Glaser, Viviane. Universidade Federal de Santa Catarina; Brasil
Fil: de Paula Martins, Roberta. Universidade Federal de Santa Catarina; Brasil
Fil: Hoffmann Vieira, Ana Julia. Universidade Federal de Santa Catarina; Brasil
Fil: de Medeiros Oliveira, Eliana. Universidade Federal de Santa Catarina; Brasil
Fil: Straliotto, Marcos Raniel. Universidade Federal de Santa Catarina; Brasil
Fil: Mukdsi, Jorge Humberto. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopia Electronica; Argentina
Fil: Torres, Alicia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopia Electronica; Argentina
Fil: Fabro de Bem, Andreza. Universidade Federal de Santa Catarina; Brasil
Fil: Farina, Marcelo. Universidade Federal de Santa Catarina; Brasil
Fil: Teixeira da Rocha, Joao Batista. Universidade Federal de Santa Maria. Santa Maria; Brasil
Fil: de Paul, Ana Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopia Electronica; Argentina
Fil: Latini, Alexandra. Universidade Federal de Santa Catarina; Brasil
Materia
Diphenyl Diselenide
Methylmercury
Mitochondrial Morphology
Creatine Kinase
Hemeoxygenase Type 1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/33427

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oai_identifier_str oai:ri.conicet.gov.ar:11336/33427
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse modelGlaser, Vivianede Paula Martins, RobertaHoffmann Vieira, Ana Juliade Medeiros Oliveira, ElianaStraliotto, Marcos RanielMukdsi, Jorge HumbertoTorres, Alicia InesFabro de Bem, AndrezaFarina, MarceloTeixeira da Rocha, Joao Batistade Paul, Ana LuciaLatini, AlexandraDiphenyl DiselenideMethylmercuryMitochondrial MorphologyCreatine KinaseHemeoxygenase Type 1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Interest in biochemistry of organoselenium compound has increased in the last decades, mainly due to their chemical and biological activities. Here, we investigated the protective effect of diphenyl diselenide (PhSe)2 (5 μmol/kg), in a mouse model of methylmercury (MeHg)-induced brain toxicity. Swiss male mice were divided into four experimental groups: control, (PhSe)2 (5 μmol/kg, subcutaneous administration), MeHg (40 mg/L, in tap water), and MeHg + (PhSe)2. After the treatment (21 days), the animals were killed and the cerebral cortex was analyzed. Electron microscopy indicated an enlarged and fused mitochondria leading to a reduced number of organelles, in the MeHg-exposed mice. Furthermore, cortical creatine kinase activity, a sensitive mitochondrial oxidative stress sensor, was almost abolished by MeHg. Subcutaneous (PhSe)2 co-treatment rescued from MeHg-induced mitochondrial alterations. (PhSe)2 also behaved as an enhancer of mitochondrial biogenesis, by increasing cortical mitochondria content in mouse-receiving (PhSe)2 alone. Mechanistically, (PhSe)2 (1 μM; 24 h) would trigger the cytoprotective Nrf-2 pathway for activating target genes, since astroglial cells exposed to the chalcogen showed increased content of hemeoxygenase type 1, a sensitive marker of the activation of this via. Thus, it is proposed that the (PhSe)2-neuroprotective effect might be linked to its mitoprotective activity.Fil: Glaser, Viviane. Universidade Federal de Santa Catarina; BrasilFil: de Paula Martins, Roberta. Universidade Federal de Santa Catarina; BrasilFil: Hoffmann Vieira, Ana Julia. Universidade Federal de Santa Catarina; BrasilFil: de Medeiros Oliveira, Eliana. Universidade Federal de Santa Catarina; BrasilFil: Straliotto, Marcos Raniel. Universidade Federal de Santa Catarina; BrasilFil: Mukdsi, Jorge Humberto. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopia Electronica; ArgentinaFil: Torres, Alicia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopia Electronica; ArgentinaFil: Fabro de Bem, Andreza. Universidade Federal de Santa Catarina; BrasilFil: Farina, Marcelo. Universidade Federal de Santa Catarina; BrasilFil: Teixeira da Rocha, Joao Batista. Universidade Federal de Santa Maria. Santa Maria; BrasilFil: de Paul, Ana Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopia Electronica; ArgentinaFil: Latini, Alexandra. Universidade Federal de Santa Catarina; BrasilSpringer2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/33427Glaser, Viviane; de Paula Martins, Roberta; Hoffmann Vieira, Ana Julia; de Medeiros Oliveira, Eliana; Straliotto, Marcos Raniel; et al.; Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse model; Springer; Molecular and Cellular Biochemistry; 390; 1-2; 3-2014; 1-80300-81771573-4919CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11010-013-1870-9info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11010-013-1870-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:17:00Zoai:ri.conicet.gov.ar:11336/33427instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:17:00.917CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse model
title Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse model
spellingShingle Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse model
Glaser, Viviane
Diphenyl Diselenide
Methylmercury
Mitochondrial Morphology
Creatine Kinase
Hemeoxygenase Type 1
title_short Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse model
title_full Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse model
title_fullStr Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse model
title_full_unstemmed Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse model
title_sort Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse model
dc.creator.none.fl_str_mv Glaser, Viviane
de Paula Martins, Roberta
Hoffmann Vieira, Ana Julia
de Medeiros Oliveira, Eliana
Straliotto, Marcos Raniel
Mukdsi, Jorge Humberto
Torres, Alicia Ines
Fabro de Bem, Andreza
Farina, Marcelo
Teixeira da Rocha, Joao Batista
de Paul, Ana Lucia
Latini, Alexandra
author Glaser, Viviane
author_facet Glaser, Viviane
de Paula Martins, Roberta
Hoffmann Vieira, Ana Julia
de Medeiros Oliveira, Eliana
Straliotto, Marcos Raniel
Mukdsi, Jorge Humberto
Torres, Alicia Ines
Fabro de Bem, Andreza
Farina, Marcelo
Teixeira da Rocha, Joao Batista
de Paul, Ana Lucia
Latini, Alexandra
author_role author
author2 de Paula Martins, Roberta
Hoffmann Vieira, Ana Julia
de Medeiros Oliveira, Eliana
Straliotto, Marcos Raniel
Mukdsi, Jorge Humberto
Torres, Alicia Ines
Fabro de Bem, Andreza
Farina, Marcelo
Teixeira da Rocha, Joao Batista
de Paul, Ana Lucia
Latini, Alexandra
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Diphenyl Diselenide
Methylmercury
Mitochondrial Morphology
Creatine Kinase
Hemeoxygenase Type 1
topic Diphenyl Diselenide
Methylmercury
Mitochondrial Morphology
Creatine Kinase
Hemeoxygenase Type 1
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Interest in biochemistry of organoselenium compound has increased in the last decades, mainly due to their chemical and biological activities. Here, we investigated the protective effect of diphenyl diselenide (PhSe)2 (5 μmol/kg), in a mouse model of methylmercury (MeHg)-induced brain toxicity. Swiss male mice were divided into four experimental groups: control, (PhSe)2 (5 μmol/kg, subcutaneous administration), MeHg (40 mg/L, in tap water), and MeHg + (PhSe)2. After the treatment (21 days), the animals were killed and the cerebral cortex was analyzed. Electron microscopy indicated an enlarged and fused mitochondria leading to a reduced number of organelles, in the MeHg-exposed mice. Furthermore, cortical creatine kinase activity, a sensitive mitochondrial oxidative stress sensor, was almost abolished by MeHg. Subcutaneous (PhSe)2 co-treatment rescued from MeHg-induced mitochondrial alterations. (PhSe)2 also behaved as an enhancer of mitochondrial biogenesis, by increasing cortical mitochondria content in mouse-receiving (PhSe)2 alone. Mechanistically, (PhSe)2 (1 μM; 24 h) would trigger the cytoprotective Nrf-2 pathway for activating target genes, since astroglial cells exposed to the chalcogen showed increased content of hemeoxygenase type 1, a sensitive marker of the activation of this via. Thus, it is proposed that the (PhSe)2-neuroprotective effect might be linked to its mitoprotective activity.
Fil: Glaser, Viviane. Universidade Federal de Santa Catarina; Brasil
Fil: de Paula Martins, Roberta. Universidade Federal de Santa Catarina; Brasil
Fil: Hoffmann Vieira, Ana Julia. Universidade Federal de Santa Catarina; Brasil
Fil: de Medeiros Oliveira, Eliana. Universidade Federal de Santa Catarina; Brasil
Fil: Straliotto, Marcos Raniel. Universidade Federal de Santa Catarina; Brasil
Fil: Mukdsi, Jorge Humberto. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopia Electronica; Argentina
Fil: Torres, Alicia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopia Electronica; Argentina
Fil: Fabro de Bem, Andreza. Universidade Federal de Santa Catarina; Brasil
Fil: Farina, Marcelo. Universidade Federal de Santa Catarina; Brasil
Fil: Teixeira da Rocha, Joao Batista. Universidade Federal de Santa Maria. Santa Maria; Brasil
Fil: de Paul, Ana Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopia Electronica; Argentina
Fil: Latini, Alexandra. Universidade Federal de Santa Catarina; Brasil
description Interest in biochemistry of organoselenium compound has increased in the last decades, mainly due to their chemical and biological activities. Here, we investigated the protective effect of diphenyl diselenide (PhSe)2 (5 μmol/kg), in a mouse model of methylmercury (MeHg)-induced brain toxicity. Swiss male mice were divided into four experimental groups: control, (PhSe)2 (5 μmol/kg, subcutaneous administration), MeHg (40 mg/L, in tap water), and MeHg + (PhSe)2. After the treatment (21 days), the animals were killed and the cerebral cortex was analyzed. Electron microscopy indicated an enlarged and fused mitochondria leading to a reduced number of organelles, in the MeHg-exposed mice. Furthermore, cortical creatine kinase activity, a sensitive mitochondrial oxidative stress sensor, was almost abolished by MeHg. Subcutaneous (PhSe)2 co-treatment rescued from MeHg-induced mitochondrial alterations. (PhSe)2 also behaved as an enhancer of mitochondrial biogenesis, by increasing cortical mitochondria content in mouse-receiving (PhSe)2 alone. Mechanistically, (PhSe)2 (1 μM; 24 h) would trigger the cytoprotective Nrf-2 pathway for activating target genes, since astroglial cells exposed to the chalcogen showed increased content of hemeoxygenase type 1, a sensitive marker of the activation of this via. Thus, it is proposed that the (PhSe)2-neuroprotective effect might be linked to its mitoprotective activity.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/33427
Glaser, Viviane; de Paula Martins, Roberta; Hoffmann Vieira, Ana Julia; de Medeiros Oliveira, Eliana; Straliotto, Marcos Raniel; et al.; Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse model; Springer; Molecular and Cellular Biochemistry; 390; 1-2; 3-2014; 1-8
0300-8177
1573-4919
CONICET Digital
CONICET
url http://hdl.handle.net/11336/33427
identifier_str_mv Glaser, Viviane; de Paula Martins, Roberta; Hoffmann Vieira, Ana Julia; de Medeiros Oliveira, Eliana; Straliotto, Marcos Raniel; et al.; Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse model; Springer; Molecular and Cellular Biochemistry; 390; 1-2; 3-2014; 1-8
0300-8177
1573-4919
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s11010-013-1870-9
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11010-013-1870-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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