Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse mod...
- Autores
- Glaser, Viviane; de Paula Martins, Roberta; Hoffmann Vieira, Ana Julia; de Medeiros Oliveira, Eliana; Straliotto, Marcos Raniel; Mukdsi, Jorge Humberto; Torres, Alicia Ines; Fabro de Bem, Andreza; Farina, Marcelo; Teixeira da Rocha, Joao Batista; de Paul, Ana Lucia; Latini, Alexandra
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Interest in biochemistry of organoselenium compound has increased in the last decades, mainly due to their chemical and biological activities. Here, we investigated the protective effect of diphenyl diselenide (PhSe)2 (5 μmol/kg), in a mouse model of methylmercury (MeHg)-induced brain toxicity. Swiss male mice were divided into four experimental groups: control, (PhSe)2 (5 μmol/kg, subcutaneous administration), MeHg (40 mg/L, in tap water), and MeHg + (PhSe)2. After the treatment (21 days), the animals were killed and the cerebral cortex was analyzed. Electron microscopy indicated an enlarged and fused mitochondria leading to a reduced number of organelles, in the MeHg-exposed mice. Furthermore, cortical creatine kinase activity, a sensitive mitochondrial oxidative stress sensor, was almost abolished by MeHg. Subcutaneous (PhSe)2 co-treatment rescued from MeHg-induced mitochondrial alterations. (PhSe)2 also behaved as an enhancer of mitochondrial biogenesis, by increasing cortical mitochondria content in mouse-receiving (PhSe)2 alone. Mechanistically, (PhSe)2 (1 μM; 24 h) would trigger the cytoprotective Nrf-2 pathway for activating target genes, since astroglial cells exposed to the chalcogen showed increased content of hemeoxygenase type 1, a sensitive marker of the activation of this via. Thus, it is proposed that the (PhSe)2-neuroprotective effect might be linked to its mitoprotective activity.
Fil: Glaser, Viviane. Universidade Federal de Santa Catarina; Brasil
Fil: de Paula Martins, Roberta. Universidade Federal de Santa Catarina; Brasil
Fil: Hoffmann Vieira, Ana Julia. Universidade Federal de Santa Catarina; Brasil
Fil: de Medeiros Oliveira, Eliana. Universidade Federal de Santa Catarina; Brasil
Fil: Straliotto, Marcos Raniel. Universidade Federal de Santa Catarina; Brasil
Fil: Mukdsi, Jorge Humberto. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopia Electronica; Argentina
Fil: Torres, Alicia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopia Electronica; Argentina
Fil: Fabro de Bem, Andreza. Universidade Federal de Santa Catarina; Brasil
Fil: Farina, Marcelo. Universidade Federal de Santa Catarina; Brasil
Fil: Teixeira da Rocha, Joao Batista. Universidade Federal de Santa Maria. Santa Maria; Brasil
Fil: de Paul, Ana Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopia Electronica; Argentina
Fil: Latini, Alexandra. Universidade Federal de Santa Catarina; Brasil - Materia
-
Diphenyl Diselenide
Methylmercury
Mitochondrial Morphology
Creatine Kinase
Hemeoxygenase Type 1 - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/33427
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oai:ri.conicet.gov.ar:11336/33427 |
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3498 |
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CONICET Digital (CONICET) |
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Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse modelGlaser, Vivianede Paula Martins, RobertaHoffmann Vieira, Ana Juliade Medeiros Oliveira, ElianaStraliotto, Marcos RanielMukdsi, Jorge HumbertoTorres, Alicia InesFabro de Bem, AndrezaFarina, MarceloTeixeira da Rocha, Joao Batistade Paul, Ana LuciaLatini, AlexandraDiphenyl DiselenideMethylmercuryMitochondrial MorphologyCreatine KinaseHemeoxygenase Type 1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Interest in biochemistry of organoselenium compound has increased in the last decades, mainly due to their chemical and biological activities. Here, we investigated the protective effect of diphenyl diselenide (PhSe)2 (5 μmol/kg), in a mouse model of methylmercury (MeHg)-induced brain toxicity. Swiss male mice were divided into four experimental groups: control, (PhSe)2 (5 μmol/kg, subcutaneous administration), MeHg (40 mg/L, in tap water), and MeHg + (PhSe)2. After the treatment (21 days), the animals were killed and the cerebral cortex was analyzed. Electron microscopy indicated an enlarged and fused mitochondria leading to a reduced number of organelles, in the MeHg-exposed mice. Furthermore, cortical creatine kinase activity, a sensitive mitochondrial oxidative stress sensor, was almost abolished by MeHg. Subcutaneous (PhSe)2 co-treatment rescued from MeHg-induced mitochondrial alterations. (PhSe)2 also behaved as an enhancer of mitochondrial biogenesis, by increasing cortical mitochondria content in mouse-receiving (PhSe)2 alone. Mechanistically, (PhSe)2 (1 μM; 24 h) would trigger the cytoprotective Nrf-2 pathway for activating target genes, since astroglial cells exposed to the chalcogen showed increased content of hemeoxygenase type 1, a sensitive marker of the activation of this via. Thus, it is proposed that the (PhSe)2-neuroprotective effect might be linked to its mitoprotective activity.Fil: Glaser, Viviane. Universidade Federal de Santa Catarina; BrasilFil: de Paula Martins, Roberta. Universidade Federal de Santa Catarina; BrasilFil: Hoffmann Vieira, Ana Julia. Universidade Federal de Santa Catarina; BrasilFil: de Medeiros Oliveira, Eliana. Universidade Federal de Santa Catarina; BrasilFil: Straliotto, Marcos Raniel. Universidade Federal de Santa Catarina; BrasilFil: Mukdsi, Jorge Humberto. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopia Electronica; ArgentinaFil: Torres, Alicia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopia Electronica; ArgentinaFil: Fabro de Bem, Andreza. Universidade Federal de Santa Catarina; BrasilFil: Farina, Marcelo. Universidade Federal de Santa Catarina; BrasilFil: Teixeira da Rocha, Joao Batista. Universidade Federal de Santa Maria. Santa Maria; BrasilFil: de Paul, Ana Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopia Electronica; ArgentinaFil: Latini, Alexandra. Universidade Federal de Santa Catarina; BrasilSpringer2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/33427Glaser, Viviane; de Paula Martins, Roberta; Hoffmann Vieira, Ana Julia; de Medeiros Oliveira, Eliana; Straliotto, Marcos Raniel; et al.; Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse model; Springer; Molecular and Cellular Biochemistry; 390; 1-2; 3-2014; 1-80300-81771573-4919CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11010-013-1870-9info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11010-013-1870-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:17:00Zoai:ri.conicet.gov.ar:11336/33427instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:17:00.917CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse model |
title |
Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse model |
spellingShingle |
Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse model Glaser, Viviane Diphenyl Diselenide Methylmercury Mitochondrial Morphology Creatine Kinase Hemeoxygenase Type 1 |
title_short |
Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse model |
title_full |
Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse model |
title_fullStr |
Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse model |
title_full_unstemmed |
Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse model |
title_sort |
Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse model |
dc.creator.none.fl_str_mv |
Glaser, Viviane de Paula Martins, Roberta Hoffmann Vieira, Ana Julia de Medeiros Oliveira, Eliana Straliotto, Marcos Raniel Mukdsi, Jorge Humberto Torres, Alicia Ines Fabro de Bem, Andreza Farina, Marcelo Teixeira da Rocha, Joao Batista de Paul, Ana Lucia Latini, Alexandra |
author |
Glaser, Viviane |
author_facet |
Glaser, Viviane de Paula Martins, Roberta Hoffmann Vieira, Ana Julia de Medeiros Oliveira, Eliana Straliotto, Marcos Raniel Mukdsi, Jorge Humberto Torres, Alicia Ines Fabro de Bem, Andreza Farina, Marcelo Teixeira da Rocha, Joao Batista de Paul, Ana Lucia Latini, Alexandra |
author_role |
author |
author2 |
de Paula Martins, Roberta Hoffmann Vieira, Ana Julia de Medeiros Oliveira, Eliana Straliotto, Marcos Raniel Mukdsi, Jorge Humberto Torres, Alicia Ines Fabro de Bem, Andreza Farina, Marcelo Teixeira da Rocha, Joao Batista de Paul, Ana Lucia Latini, Alexandra |
author2_role |
author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Diphenyl Diselenide Methylmercury Mitochondrial Morphology Creatine Kinase Hemeoxygenase Type 1 |
topic |
Diphenyl Diselenide Methylmercury Mitochondrial Morphology Creatine Kinase Hemeoxygenase Type 1 |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Interest in biochemistry of organoselenium compound has increased in the last decades, mainly due to their chemical and biological activities. Here, we investigated the protective effect of diphenyl diselenide (PhSe)2 (5 μmol/kg), in a mouse model of methylmercury (MeHg)-induced brain toxicity. Swiss male mice were divided into four experimental groups: control, (PhSe)2 (5 μmol/kg, subcutaneous administration), MeHg (40 mg/L, in tap water), and MeHg + (PhSe)2. After the treatment (21 days), the animals were killed and the cerebral cortex was analyzed. Electron microscopy indicated an enlarged and fused mitochondria leading to a reduced number of organelles, in the MeHg-exposed mice. Furthermore, cortical creatine kinase activity, a sensitive mitochondrial oxidative stress sensor, was almost abolished by MeHg. Subcutaneous (PhSe)2 co-treatment rescued from MeHg-induced mitochondrial alterations. (PhSe)2 also behaved as an enhancer of mitochondrial biogenesis, by increasing cortical mitochondria content in mouse-receiving (PhSe)2 alone. Mechanistically, (PhSe)2 (1 μM; 24 h) would trigger the cytoprotective Nrf-2 pathway for activating target genes, since astroglial cells exposed to the chalcogen showed increased content of hemeoxygenase type 1, a sensitive marker of the activation of this via. Thus, it is proposed that the (PhSe)2-neuroprotective effect might be linked to its mitoprotective activity. Fil: Glaser, Viviane. Universidade Federal de Santa Catarina; Brasil Fil: de Paula Martins, Roberta. Universidade Federal de Santa Catarina; Brasil Fil: Hoffmann Vieira, Ana Julia. Universidade Federal de Santa Catarina; Brasil Fil: de Medeiros Oliveira, Eliana. Universidade Federal de Santa Catarina; Brasil Fil: Straliotto, Marcos Raniel. Universidade Federal de Santa Catarina; Brasil Fil: Mukdsi, Jorge Humberto. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopia Electronica; Argentina Fil: Torres, Alicia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopia Electronica; Argentina Fil: Fabro de Bem, Andreza. Universidade Federal de Santa Catarina; Brasil Fil: Farina, Marcelo. Universidade Federal de Santa Catarina; Brasil Fil: Teixeira da Rocha, Joao Batista. Universidade Federal de Santa Maria. Santa Maria; Brasil Fil: de Paul, Ana Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopia Electronica; Argentina Fil: Latini, Alexandra. Universidade Federal de Santa Catarina; Brasil |
description |
Interest in biochemistry of organoselenium compound has increased in the last decades, mainly due to their chemical and biological activities. Here, we investigated the protective effect of diphenyl diselenide (PhSe)2 (5 μmol/kg), in a mouse model of methylmercury (MeHg)-induced brain toxicity. Swiss male mice were divided into four experimental groups: control, (PhSe)2 (5 μmol/kg, subcutaneous administration), MeHg (40 mg/L, in tap water), and MeHg + (PhSe)2. After the treatment (21 days), the animals were killed and the cerebral cortex was analyzed. Electron microscopy indicated an enlarged and fused mitochondria leading to a reduced number of organelles, in the MeHg-exposed mice. Furthermore, cortical creatine kinase activity, a sensitive mitochondrial oxidative stress sensor, was almost abolished by MeHg. Subcutaneous (PhSe)2 co-treatment rescued from MeHg-induced mitochondrial alterations. (PhSe)2 also behaved as an enhancer of mitochondrial biogenesis, by increasing cortical mitochondria content in mouse-receiving (PhSe)2 alone. Mechanistically, (PhSe)2 (1 μM; 24 h) would trigger the cytoprotective Nrf-2 pathway for activating target genes, since astroglial cells exposed to the chalcogen showed increased content of hemeoxygenase type 1, a sensitive marker of the activation of this via. Thus, it is proposed that the (PhSe)2-neuroprotective effect might be linked to its mitoprotective activity. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/33427 Glaser, Viviane; de Paula Martins, Roberta; Hoffmann Vieira, Ana Julia; de Medeiros Oliveira, Eliana; Straliotto, Marcos Raniel; et al.; Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse model; Springer; Molecular and Cellular Biochemistry; 390; 1-2; 3-2014; 1-8 0300-8177 1573-4919 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/33427 |
identifier_str_mv |
Glaser, Viviane; de Paula Martins, Roberta; Hoffmann Vieira, Ana Julia; de Medeiros Oliveira, Eliana; Straliotto, Marcos Raniel; et al.; Diphenyl diselenide administration enhances cortical mitochondrial number and activity by increasing hemeoxygenase type 1 content in a methylmercury-induced neurotoxicity mouse model; Springer; Molecular and Cellular Biochemistry; 390; 1-2; 3-2014; 1-8 0300-8177 1573-4919 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11010-013-1870-9 info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11010-013-1870-9 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844614119957725184 |
score |
13.070432 |