Diphenyl diselenide-modulation of macrophage activation: Down-regulation of classical and alternative activation markers

Autores
Rupil, Lucia; de Bem, Andreza F.; Roth, German Alfredo
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Diphenyl diselenide ((PhSe)2), a simple organoselenium compound, possesses interesting pharmacological properties that are under extensive research. As macrophages respond to microenvironmental stimuli and can display activities engaged in the initiation and the resolution of inflammation, in the present report we describe the ability of (PhSe)2 to modulate the macrophage activation. Our data indicate that (PhSe)2 could inhibit the NO production in a dose-dependent fashion in peritoneal macrophages activated by LPS or treated with vehicle alone. We could demonstrate that this effect correlated with a reduction in the expression of the inducible NO synthase in (PhSe)2-treated cells. Furthermore, (PhSe)2 suppressed the production of reactive oxygen species, diminished the activity of the arginase enzyme, and the accumulation of nitrotyrosine modified proteins in LPS-stimulated macrophages. This compound also diminished the antigen presentation capacity of classically activated macrophages, as it reduced MHCII and CD86 expression. In addition, (PhSe)2 modulated the alternative activation phenotype of macrophages. Dexamethasone-activated macrophages presented higher production of IL-10 and CD206, which were both down-regulated by the addition of (PhSe)2. These results suggest that (PhSe)2 possesses antioxidant and anti-inflammatory activities in classically-activated macrophages. We could demonstrate that (PhSe)2 can be also utilized to modulate the alternative activation phenotype of macrophages.
Fil: Rupil, Lucia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas. Universidad Católica de Córdoba. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas; Argentina
Fil: de Bem, Andreza F.. Universidade Federal de Santa Catarina; Brasil
Fil: Roth, German Alfredo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Materia
Macrophages
Arginase
NO
Diphenyl diselenide
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/133797
Acceder
id CONICETDig_09d8683dc181336923c081dcfc3ba205
oai_identifier_str oai:ri.conicet.gov.ar:11336/133797
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Diphenyl diselenide-modulation of macrophage activation: Down-regulation of classical and alternative activation markersRupil, Luciade Bem, Andreza F.Roth, German AlfredoMacrophagesArginaseNODiphenyl diselenidehttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Diphenyl diselenide ((PhSe)2), a simple organoselenium compound, possesses interesting pharmacological properties that are under extensive research. As macrophages respond to microenvironmental stimuli and can display activities engaged in the initiation and the resolution of inflammation, in the present report we describe the ability of (PhSe)2 to modulate the macrophage activation. Our data indicate that (PhSe)2 could inhibit the NO production in a dose-dependent fashion in peritoneal macrophages activated by LPS or treated with vehicle alone. We could demonstrate that this effect correlated with a reduction in the expression of the inducible NO synthase in (PhSe)2-treated cells. Furthermore, (PhSe)2 suppressed the production of reactive oxygen species, diminished the activity of the arginase enzyme, and the accumulation of nitrotyrosine modified proteins in LPS-stimulated macrophages. This compound also diminished the antigen presentation capacity of classically activated macrophages, as it reduced MHCII and CD86 expression. In addition, (PhSe)2 modulated the alternative activation phenotype of macrophages. Dexamethasone-activated macrophages presented higher production of IL-10 and CD206, which were both down-regulated by the addition of (PhSe)2. These results suggest that (PhSe)2 possesses antioxidant and anti-inflammatory activities in classically-activated macrophages. We could demonstrate that (PhSe)2 can be also utilized to modulate the alternative activation phenotype of macrophages.Fil: Rupil, Lucia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas. Universidad Católica de Córdoba. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas; ArgentinaFil: de Bem, Andreza F.. Universidade Federal de Santa Catarina; BrasilFil: Roth, German Alfredo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaSage Publications Ltd2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/133797Rupil, Lucia; de Bem, Andreza F.; Roth, German Alfredo; Diphenyl diselenide-modulation of macrophage activation: Down-regulation of classical and alternative activation markers; Sage Publications Ltd; Innate Immunity; 18; 4; 1-2012; 627-6371753-42591753-4267CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://ini.sagepub.com/content/early/2012/01/03/1753425911431285info:eu-repo/semantics/altIdentifier/doi/10.1177%2F1753425911431285info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:22:48Zoai:ri.conicet.gov.ar:11336/133797instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:22:48.552CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Diphenyl diselenide-modulation of macrophage activation: Down-regulation of classical and alternative activation markers
title Diphenyl diselenide-modulation of macrophage activation: Down-regulation of classical and alternative activation markers
spellingShingle Diphenyl diselenide-modulation of macrophage activation: Down-regulation of classical and alternative activation markers
Rupil, Lucia
Macrophages
Arginase
NO
Diphenyl diselenide
title_short Diphenyl diselenide-modulation of macrophage activation: Down-regulation of classical and alternative activation markers
title_full Diphenyl diselenide-modulation of macrophage activation: Down-regulation of classical and alternative activation markers
title_fullStr Diphenyl diselenide-modulation of macrophage activation: Down-regulation of classical and alternative activation markers
title_full_unstemmed Diphenyl diselenide-modulation of macrophage activation: Down-regulation of classical and alternative activation markers
title_sort Diphenyl diselenide-modulation of macrophage activation: Down-regulation of classical and alternative activation markers
dc.creator.none.fl_str_mv Rupil, Lucia
de Bem, Andreza F.
Roth, German Alfredo
author Rupil, Lucia
author_facet Rupil, Lucia
de Bem, Andreza F.
Roth, German Alfredo
author_role author
author2 de Bem, Andreza F.
Roth, German Alfredo
author2_role author
author
dc.subject.none.fl_str_mv Macrophages
Arginase
NO
Diphenyl diselenide
topic Macrophages
Arginase
NO
Diphenyl diselenide
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Diphenyl diselenide ((PhSe)2), a simple organoselenium compound, possesses interesting pharmacological properties that are under extensive research. As macrophages respond to microenvironmental stimuli and can display activities engaged in the initiation and the resolution of inflammation, in the present report we describe the ability of (PhSe)2 to modulate the macrophage activation. Our data indicate that (PhSe)2 could inhibit the NO production in a dose-dependent fashion in peritoneal macrophages activated by LPS or treated with vehicle alone. We could demonstrate that this effect correlated with a reduction in the expression of the inducible NO synthase in (PhSe)2-treated cells. Furthermore, (PhSe)2 suppressed the production of reactive oxygen species, diminished the activity of the arginase enzyme, and the accumulation of nitrotyrosine modified proteins in LPS-stimulated macrophages. This compound also diminished the antigen presentation capacity of classically activated macrophages, as it reduced MHCII and CD86 expression. In addition, (PhSe)2 modulated the alternative activation phenotype of macrophages. Dexamethasone-activated macrophages presented higher production of IL-10 and CD206, which were both down-regulated by the addition of (PhSe)2. These results suggest that (PhSe)2 possesses antioxidant and anti-inflammatory activities in classically-activated macrophages. We could demonstrate that (PhSe)2 can be also utilized to modulate the alternative activation phenotype of macrophages.
Fil: Rupil, Lucia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas. Universidad Católica de Córdoba. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas; Argentina
Fil: de Bem, Andreza F.. Universidade Federal de Santa Catarina; Brasil
Fil: Roth, German Alfredo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
description Diphenyl diselenide ((PhSe)2), a simple organoselenium compound, possesses interesting pharmacological properties that are under extensive research. As macrophages respond to microenvironmental stimuli and can display activities engaged in the initiation and the resolution of inflammation, in the present report we describe the ability of (PhSe)2 to modulate the macrophage activation. Our data indicate that (PhSe)2 could inhibit the NO production in a dose-dependent fashion in peritoneal macrophages activated by LPS or treated with vehicle alone. We could demonstrate that this effect correlated with a reduction in the expression of the inducible NO synthase in (PhSe)2-treated cells. Furthermore, (PhSe)2 suppressed the production of reactive oxygen species, diminished the activity of the arginase enzyme, and the accumulation of nitrotyrosine modified proteins in LPS-stimulated macrophages. This compound also diminished the antigen presentation capacity of classically activated macrophages, as it reduced MHCII and CD86 expression. In addition, (PhSe)2 modulated the alternative activation phenotype of macrophages. Dexamethasone-activated macrophages presented higher production of IL-10 and CD206, which were both down-regulated by the addition of (PhSe)2. These results suggest that (PhSe)2 possesses antioxidant and anti-inflammatory activities in classically-activated macrophages. We could demonstrate that (PhSe)2 can be also utilized to modulate the alternative activation phenotype of macrophages.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/133797
Rupil, Lucia; de Bem, Andreza F.; Roth, German Alfredo; Diphenyl diselenide-modulation of macrophage activation: Down-regulation of classical and alternative activation markers; Sage Publications Ltd; Innate Immunity; 18; 4; 1-2012; 627-637
1753-4259
1753-4267
CONICET Digital
CONICET
url http://hdl.handle.net/11336/133797
identifier_str_mv Rupil, Lucia; de Bem, Andreza F.; Roth, German Alfredo; Diphenyl diselenide-modulation of macrophage activation: Down-regulation of classical and alternative activation markers; Sage Publications Ltd; Innate Immunity; 18; 4; 1-2012; 627-637
1753-4259
1753-4267
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://ini.sagepub.com/content/early/2012/01/03/1753425911431285
info:eu-repo/semantics/altIdentifier/doi/10.1177%2F1753425911431285
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sage Publications Ltd
publisher.none.fl_str_mv Sage Publications Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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