Bovine embryo-oviduct interaction in vitro reveals an early cross talk mediated by BMP signaling

Autores
Garcia, Elina Vanesa; Hamdi, Meriem; Barrera, Antonio Daniel; Sánchez Calabuig, María Jesús; Gutiérrez Adan, Alfonso; Rizos, Dimitrios
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Signaling components of bone morphogenetic proteins (BMPs) are expressed in an anatomically and temporally regulated fashion in bovine oviduct. However, a local response of this signaling to the presence of the embryo has yet to be elucidated. The aim of the present study was to evaluate if early embryo-oviduct interaction induces changes in the gene expression of BMP signaling components. For this purpose, we used an in vitro co-culture system to investigate the local interaction between bovine oviductal epithelial cells (BOEC) from the isthmus region with early embryos during two developmental periods: before (from the 2-cell to 8-cell stage) or during (from the 8-cell to 16-cell stage) the main phase of embryonic genome activation (EGA). Exposure to embryos, irrespective of the period, significantly reduced the relative abundance of BMPR1B, BMPR2, SMAD1, SMAD6 and ID2 mRNAs in BOEC. In contrast, embryos that interacted with BOEC before EGA showed a significant increase in the relative abundance of SMAD1 mRNA at the 8-cell stage compared to embryos cultured without BOEC. Moreover, embryos at the 16-cell stage that interacted with BOEC during EGA showed a significant increase in BMPR1B, BMPR2 and ID2 mRNA. These results demonstrate that embryo-oviduct interaction in vitro induces specific changes in the transcriptional levels of BMP signaling, causing a bidirectional response that reduces the expression levels of this signaling in the oviductal cells while increases them in the early embryo. This suggests that BMP signaling pathway could be involved in an early cross talk between the bovine embryo and the oviduct during the first stages of development.
Fil: Garcia, Elina Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria; España
Fil: Hamdi, Meriem. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria; España
Fil: Barrera, Antonio Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria; España
Fil: Sánchez Calabuig, María Jesús. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria; España
Fil: Gutiérrez Adan, Alfonso. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria; España
Fil: Rizos, Dimitrios. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria; España
Materia
BMP
BOVINE
IN VITRO CO-CULTURE
OVIDUCT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53717
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/53717
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bovine embryo-oviduct interaction in vitro reveals an early cross talk mediated by BMP signalingGarcia, Elina VanesaHamdi, MeriemBarrera, Antonio DanielSánchez Calabuig, María JesúsGutiérrez Adan, AlfonsoRizos, DimitriosBMPBOVINEIN VITRO CO-CULTUREOVIDUCThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Signaling components of bone morphogenetic proteins (BMPs) are expressed in an anatomically and temporally regulated fashion in bovine oviduct. However, a local response of this signaling to the presence of the embryo has yet to be elucidated. The aim of the present study was to evaluate if early embryo-oviduct interaction induces changes in the gene expression of BMP signaling components. For this purpose, we used an in vitro co-culture system to investigate the local interaction between bovine oviductal epithelial cells (BOEC) from the isthmus region with early embryos during two developmental periods: before (from the 2-cell to 8-cell stage) or during (from the 8-cell to 16-cell stage) the main phase of embryonic genome activation (EGA). Exposure to embryos, irrespective of the period, significantly reduced the relative abundance of BMPR1B, BMPR2, SMAD1, SMAD6 and ID2 mRNAs in BOEC. In contrast, embryos that interacted with BOEC before EGA showed a significant increase in the relative abundance of SMAD1 mRNA at the 8-cell stage compared to embryos cultured without BOEC. Moreover, embryos at the 16-cell stage that interacted with BOEC during EGA showed a significant increase in BMPR1B, BMPR2 and ID2 mRNA. These results demonstrate that embryo-oviduct interaction in vitro induces specific changes in the transcriptional levels of BMP signaling, causing a bidirectional response that reduces the expression levels of this signaling in the oviductal cells while increases them in the early embryo. This suggests that BMP signaling pathway could be involved in an early cross talk between the bovine embryo and the oviduct during the first stages of development.Fil: Garcia, Elina Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria; EspañaFil: Hamdi, Meriem. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria; EspañaFil: Barrera, Antonio Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria; EspañaFil: Sánchez Calabuig, María Jesús. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria; EspañaFil: Gutiérrez Adan, Alfonso. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria; EspañaFil: Rizos, Dimitrios. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria; EspañaBioScientifica2017-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53717Garcia, Elina Vanesa; Hamdi, Meriem; Barrera, Antonio Daniel; Sánchez Calabuig, María Jesús; Gutiérrez Adan, Alfonso; et al.; Bovine embryo-oviduct interaction in vitro reveals an early cross talk mediated by BMP signaling; BioScientifica; Reproduction; 153; 5; 5-2017; 631-6431470-1626CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1530/REP-16-0654info:eu-repo/semantics/altIdentifier/url/http://www.reproduction-online.org/content/153/5/631info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:31Zoai:ri.conicet.gov.ar:11336/53717instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:31.661CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bovine embryo-oviduct interaction in vitro reveals an early cross talk mediated by BMP signaling
title Bovine embryo-oviduct interaction in vitro reveals an early cross talk mediated by BMP signaling
spellingShingle Bovine embryo-oviduct interaction in vitro reveals an early cross talk mediated by BMP signaling
Garcia, Elina Vanesa
BMP
BOVINE
IN VITRO CO-CULTURE
OVIDUCT
title_short Bovine embryo-oviduct interaction in vitro reveals an early cross talk mediated by BMP signaling
title_full Bovine embryo-oviduct interaction in vitro reveals an early cross talk mediated by BMP signaling
title_fullStr Bovine embryo-oviduct interaction in vitro reveals an early cross talk mediated by BMP signaling
title_full_unstemmed Bovine embryo-oviduct interaction in vitro reveals an early cross talk mediated by BMP signaling
title_sort Bovine embryo-oviduct interaction in vitro reveals an early cross talk mediated by BMP signaling
dc.creator.none.fl_str_mv Garcia, Elina Vanesa
Hamdi, Meriem
Barrera, Antonio Daniel
Sánchez Calabuig, María Jesús
Gutiérrez Adan, Alfonso
Rizos, Dimitrios
author Garcia, Elina Vanesa
author_facet Garcia, Elina Vanesa
Hamdi, Meriem
Barrera, Antonio Daniel
Sánchez Calabuig, María Jesús
Gutiérrez Adan, Alfonso
Rizos, Dimitrios
author_role author
author2 Hamdi, Meriem
Barrera, Antonio Daniel
Sánchez Calabuig, María Jesús
Gutiérrez Adan, Alfonso
Rizos, Dimitrios
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BMP
BOVINE
IN VITRO CO-CULTURE
OVIDUCT
topic BMP
BOVINE
IN VITRO CO-CULTURE
OVIDUCT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Signaling components of bone morphogenetic proteins (BMPs) are expressed in an anatomically and temporally regulated fashion in bovine oviduct. However, a local response of this signaling to the presence of the embryo has yet to be elucidated. The aim of the present study was to evaluate if early embryo-oviduct interaction induces changes in the gene expression of BMP signaling components. For this purpose, we used an in vitro co-culture system to investigate the local interaction between bovine oviductal epithelial cells (BOEC) from the isthmus region with early embryos during two developmental periods: before (from the 2-cell to 8-cell stage) or during (from the 8-cell to 16-cell stage) the main phase of embryonic genome activation (EGA). Exposure to embryos, irrespective of the period, significantly reduced the relative abundance of BMPR1B, BMPR2, SMAD1, SMAD6 and ID2 mRNAs in BOEC. In contrast, embryos that interacted with BOEC before EGA showed a significant increase in the relative abundance of SMAD1 mRNA at the 8-cell stage compared to embryos cultured without BOEC. Moreover, embryos at the 16-cell stage that interacted with BOEC during EGA showed a significant increase in BMPR1B, BMPR2 and ID2 mRNA. These results demonstrate that embryo-oviduct interaction in vitro induces specific changes in the transcriptional levels of BMP signaling, causing a bidirectional response that reduces the expression levels of this signaling in the oviductal cells while increases them in the early embryo. This suggests that BMP signaling pathway could be involved in an early cross talk between the bovine embryo and the oviduct during the first stages of development.
Fil: Garcia, Elina Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria; España
Fil: Hamdi, Meriem. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria; España
Fil: Barrera, Antonio Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria; España
Fil: Sánchez Calabuig, María Jesús. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria; España
Fil: Gutiérrez Adan, Alfonso. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria; España
Fil: Rizos, Dimitrios. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria; España
description Signaling components of bone morphogenetic proteins (BMPs) are expressed in an anatomically and temporally regulated fashion in bovine oviduct. However, a local response of this signaling to the presence of the embryo has yet to be elucidated. The aim of the present study was to evaluate if early embryo-oviduct interaction induces changes in the gene expression of BMP signaling components. For this purpose, we used an in vitro co-culture system to investigate the local interaction between bovine oviductal epithelial cells (BOEC) from the isthmus region with early embryos during two developmental periods: before (from the 2-cell to 8-cell stage) or during (from the 8-cell to 16-cell stage) the main phase of embryonic genome activation (EGA). Exposure to embryos, irrespective of the period, significantly reduced the relative abundance of BMPR1B, BMPR2, SMAD1, SMAD6 and ID2 mRNAs in BOEC. In contrast, embryos that interacted with BOEC before EGA showed a significant increase in the relative abundance of SMAD1 mRNA at the 8-cell stage compared to embryos cultured without BOEC. Moreover, embryos at the 16-cell stage that interacted with BOEC during EGA showed a significant increase in BMPR1B, BMPR2 and ID2 mRNA. These results demonstrate that embryo-oviduct interaction in vitro induces specific changes in the transcriptional levels of BMP signaling, causing a bidirectional response that reduces the expression levels of this signaling in the oviductal cells while increases them in the early embryo. This suggests that BMP signaling pathway could be involved in an early cross talk between the bovine embryo and the oviduct during the first stages of development.
publishDate 2017
dc.date.none.fl_str_mv 2017-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53717
Garcia, Elina Vanesa; Hamdi, Meriem; Barrera, Antonio Daniel; Sánchez Calabuig, María Jesús; Gutiérrez Adan, Alfonso; et al.; Bovine embryo-oviduct interaction in vitro reveals an early cross talk mediated by BMP signaling; BioScientifica; Reproduction; 153; 5; 5-2017; 631-643
1470-1626
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53717
identifier_str_mv Garcia, Elina Vanesa; Hamdi, Meriem; Barrera, Antonio Daniel; Sánchez Calabuig, María Jesús; Gutiérrez Adan, Alfonso; et al.; Bovine embryo-oviduct interaction in vitro reveals an early cross talk mediated by BMP signaling; BioScientifica; Reproduction; 153; 5; 5-2017; 631-643
1470-1626
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1530/REP-16-0654
info:eu-repo/semantics/altIdentifier/url/http://www.reproduction-online.org/content/153/5/631
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioScientifica
publisher.none.fl_str_mv BioScientifica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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