Chromaffin and sustentacular cells in the adrenal medulla of the viscacha (Lagostomus maximus maximus): A histochemical and immunohistochemical study
- Autores
- Gallol, Luis Ezequiel; Mohamed, Fabian Heber
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Chromaffin and sustentacular cells are common components of the adrenal medulla of mammalian species. However, subpopulations of these cells varies among the different species. While the rat adrenal medulla is known to contain noradrenergic chromaffin cells in close relation with GFAP (glial fibrillary acidic protein)-positive sustentacular cells (SC-GFAP) other species express only chromaffin cells with an adrenergic phenotype and lack of SC-GFAPir. The aim of this study is to assess the distribution of adrenergic and noradrenergic chromaffin cells and SC-GFAPir in the adrenal medulla of the viscacha. Four male viscachas (N=4) and four male rats (N=4; technique control) were used in this study. In order to study the presence of adrenergic and noradrenergic chromaffin cells in the adrenal medulla, we used the histochemical method described by Honoré. The presence of SC-GFAPir was study by immunohistochemistry using an antibody against GFAP. In our study, the histochemical detection of noradrenergic chromaffin cells was negative throughout the adrenal medulla of the viscacha suggesting an adrenergic phenotype for this species, while the rat adrenal medulla used as control showed a strong positive reaction. The immunohistochemical detection of SC-GFAPir was also negative in the viscacha. These results showed that the viscacha adrenal medulla contains only chromaffin cells with an adrenergic phenotype and lack of SCGFAPir. This study is in accordance and contributes with the current literature adding another observation that suggests that SC-GFAPir are only expressed in presence of chromaffin cells with a noradrenergic phenotype. However, further studies are necessary to confirm this relation.
Fil: Gallol, Luis Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Mohamed, Fabian Heber. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
XXXV Reunión Científica Anual de la Sociedad de Biología de Cuyo
Villa de Merlo
Argentina
Sociedad de Biología de Cuyo - Materia
-
ADRENAL GLAND
CHROMAFFIN CELLS
HISTOCHEMICAL STUDY
VISCACHA - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/162752
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Chromaffin and sustentacular cells in the adrenal medulla of the viscacha (Lagostomus maximus maximus): A histochemical and immunohistochemical studyGallol, Luis EzequielMohamed, Fabian HeberADRENAL GLANDCHROMAFFIN CELLSHISTOCHEMICAL STUDYVISCACHAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Chromaffin and sustentacular cells are common components of the adrenal medulla of mammalian species. However, subpopulations of these cells varies among the different species. While the rat adrenal medulla is known to contain noradrenergic chromaffin cells in close relation with GFAP (glial fibrillary acidic protein)-positive sustentacular cells (SC-GFAP) other species express only chromaffin cells with an adrenergic phenotype and lack of SC-GFAPir. The aim of this study is to assess the distribution of adrenergic and noradrenergic chromaffin cells and SC-GFAPir in the adrenal medulla of the viscacha. Four male viscachas (N=4) and four male rats (N=4; technique control) were used in this study. In order to study the presence of adrenergic and noradrenergic chromaffin cells in the adrenal medulla, we used the histochemical method described by Honoré. The presence of SC-GFAPir was study by immunohistochemistry using an antibody against GFAP. In our study, the histochemical detection of noradrenergic chromaffin cells was negative throughout the adrenal medulla of the viscacha suggesting an adrenergic phenotype for this species, while the rat adrenal medulla used as control showed a strong positive reaction. The immunohistochemical detection of SC-GFAPir was also negative in the viscacha. These results showed that the viscacha adrenal medulla contains only chromaffin cells with an adrenergic phenotype and lack of SCGFAPir. This study is in accordance and contributes with the current literature adding another observation that suggests that SC-GFAPir are only expressed in presence of chromaffin cells with a noradrenergic phenotype. However, further studies are necessary to confirm this relation.Fil: Gallol, Luis Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Mohamed, Fabian Heber. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaXXXV Reunión Científica Anual de la Sociedad de Biología de CuyoVilla de MerloArgentinaSociedad de Biología de CuyoSociedad de Biología de Cuyo2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/162752Chromaffin and sustentacular cells in the adrenal medulla of the viscacha (Lagostomus maximus maximus): A histochemical and immunohistochemical study; XXXV Reunión Científica Anual de la Sociedad de Biología de Cuyo; Villa de Merlo; Argentina; 2017; 31-31CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/reuniones-anuales-anteriores/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:33:35Zoai:ri.conicet.gov.ar:11336/162752instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:33:36.204CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Chromaffin and sustentacular cells in the adrenal medulla of the viscacha (Lagostomus maximus maximus): A histochemical and immunohistochemical study |
title |
Chromaffin and sustentacular cells in the adrenal medulla of the viscacha (Lagostomus maximus maximus): A histochemical and immunohistochemical study |
spellingShingle |
Chromaffin and sustentacular cells in the adrenal medulla of the viscacha (Lagostomus maximus maximus): A histochemical and immunohistochemical study Gallol, Luis Ezequiel ADRENAL GLAND CHROMAFFIN CELLS HISTOCHEMICAL STUDY VISCACHA |
title_short |
Chromaffin and sustentacular cells in the adrenal medulla of the viscacha (Lagostomus maximus maximus): A histochemical and immunohistochemical study |
title_full |
Chromaffin and sustentacular cells in the adrenal medulla of the viscacha (Lagostomus maximus maximus): A histochemical and immunohistochemical study |
title_fullStr |
Chromaffin and sustentacular cells in the adrenal medulla of the viscacha (Lagostomus maximus maximus): A histochemical and immunohistochemical study |
title_full_unstemmed |
Chromaffin and sustentacular cells in the adrenal medulla of the viscacha (Lagostomus maximus maximus): A histochemical and immunohistochemical study |
title_sort |
Chromaffin and sustentacular cells in the adrenal medulla of the viscacha (Lagostomus maximus maximus): A histochemical and immunohistochemical study |
dc.creator.none.fl_str_mv |
Gallol, Luis Ezequiel Mohamed, Fabian Heber |
author |
Gallol, Luis Ezequiel |
author_facet |
Gallol, Luis Ezequiel Mohamed, Fabian Heber |
author_role |
author |
author2 |
Mohamed, Fabian Heber |
author2_role |
author |
dc.subject.none.fl_str_mv |
ADRENAL GLAND CHROMAFFIN CELLS HISTOCHEMICAL STUDY VISCACHA |
topic |
ADRENAL GLAND CHROMAFFIN CELLS HISTOCHEMICAL STUDY VISCACHA |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Chromaffin and sustentacular cells are common components of the adrenal medulla of mammalian species. However, subpopulations of these cells varies among the different species. While the rat adrenal medulla is known to contain noradrenergic chromaffin cells in close relation with GFAP (glial fibrillary acidic protein)-positive sustentacular cells (SC-GFAP) other species express only chromaffin cells with an adrenergic phenotype and lack of SC-GFAPir. The aim of this study is to assess the distribution of adrenergic and noradrenergic chromaffin cells and SC-GFAPir in the adrenal medulla of the viscacha. Four male viscachas (N=4) and four male rats (N=4; technique control) were used in this study. In order to study the presence of adrenergic and noradrenergic chromaffin cells in the adrenal medulla, we used the histochemical method described by Honoré. The presence of SC-GFAPir was study by immunohistochemistry using an antibody against GFAP. In our study, the histochemical detection of noradrenergic chromaffin cells was negative throughout the adrenal medulla of the viscacha suggesting an adrenergic phenotype for this species, while the rat adrenal medulla used as control showed a strong positive reaction. The immunohistochemical detection of SC-GFAPir was also negative in the viscacha. These results showed that the viscacha adrenal medulla contains only chromaffin cells with an adrenergic phenotype and lack of SCGFAPir. This study is in accordance and contributes with the current literature adding another observation that suggests that SC-GFAPir are only expressed in presence of chromaffin cells with a noradrenergic phenotype. However, further studies are necessary to confirm this relation. Fil: Gallol, Luis Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Mohamed, Fabian Heber. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina XXXV Reunión Científica Anual de la Sociedad de Biología de Cuyo Villa de Merlo Argentina Sociedad de Biología de Cuyo |
description |
Chromaffin and sustentacular cells are common components of the adrenal medulla of mammalian species. However, subpopulations of these cells varies among the different species. While the rat adrenal medulla is known to contain noradrenergic chromaffin cells in close relation with GFAP (glial fibrillary acidic protein)-positive sustentacular cells (SC-GFAP) other species express only chromaffin cells with an adrenergic phenotype and lack of SC-GFAPir. The aim of this study is to assess the distribution of adrenergic and noradrenergic chromaffin cells and SC-GFAPir in the adrenal medulla of the viscacha. Four male viscachas (N=4) and four male rats (N=4; technique control) were used in this study. In order to study the presence of adrenergic and noradrenergic chromaffin cells in the adrenal medulla, we used the histochemical method described by Honoré. The presence of SC-GFAPir was study by immunohistochemistry using an antibody against GFAP. In our study, the histochemical detection of noradrenergic chromaffin cells was negative throughout the adrenal medulla of the viscacha suggesting an adrenergic phenotype for this species, while the rat adrenal medulla used as control showed a strong positive reaction. The immunohistochemical detection of SC-GFAPir was also negative in the viscacha. These results showed that the viscacha adrenal medulla contains only chromaffin cells with an adrenergic phenotype and lack of SCGFAPir. This study is in accordance and contributes with the current literature adding another observation that suggests that SC-GFAPir are only expressed in presence of chromaffin cells with a noradrenergic phenotype. However, further studies are necessary to confirm this relation. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Book http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/162752 Chromaffin and sustentacular cells in the adrenal medulla of the viscacha (Lagostomus maximus maximus): A histochemical and immunohistochemical study; XXXV Reunión Científica Anual de la Sociedad de Biología de Cuyo; Villa de Merlo; Argentina; 2017; 31-31 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/162752 |
identifier_str_mv |
Chromaffin and sustentacular cells in the adrenal medulla of the viscacha (Lagostomus maximus maximus): A histochemical and immunohistochemical study; XXXV Reunión Científica Anual de la Sociedad de Biología de Cuyo; Villa de Merlo; Argentina; 2017; 31-31 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/reuniones-anuales-anteriores/ |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.coverage.none.fl_str_mv |
Nacional |
dc.publisher.none.fl_str_mv |
Sociedad de Biología de Cuyo |
publisher.none.fl_str_mv |
Sociedad de Biología de Cuyo |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614351465480192 |
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13.070432 |