Chromaffin and sustentacular cells in the adrenal medulla of the viscacha (Lagostomus maximus maximus): A histochemical and immunohistochemical study

Autores
Gallol, Luis Ezequiel; Mohamed, Fabian Heber
Año de publicación
2017
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Chromaffin and sustentacular cells are common components of the adrenal medulla of mammalian species. However, subpopulations of these cells varies among the different species. While the rat adrenal medulla is known to contain noradrenergic chromaffin cells in close relation with GFAP (glial fibrillary acidic protein)-positive sustentacular cells (SC-GFAP) other species express only chromaffin cells with an adrenergic phenotype and lack of SC-GFAPir. The aim of this study is to assess the distribution of adrenergic and noradrenergic chromaffin cells and SC-GFAPir in the adrenal medulla of the viscacha. Four male viscachas (N=4) and four male rats (N=4; technique control) were used in this study. In order to study the presence of adrenergic and noradrenergic chromaffin cells in the adrenal medulla, we used the histochemical method described by Honoré. The presence of SC-GFAPir was study by immunohistochemistry using an antibody against GFAP. In our study, the histochemical detection of noradrenergic chromaffin cells was negative throughout the adrenal medulla of the viscacha suggesting an adrenergic phenotype for this species, while the rat adrenal medulla used as control showed a strong positive reaction. The immunohistochemical detection of SC-GFAPir was also negative in the viscacha. These results showed that the viscacha adrenal medulla contains only chromaffin cells with an adrenergic phenotype and lack of SCGFAPir. This study is in accordance and contributes with the current literature adding another observation that suggests that SC-GFAPir are only expressed in presence of chromaffin cells with a noradrenergic phenotype. However, further studies are necessary to confirm this relation.
Fil: Gallol, Luis Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Mohamed, Fabian Heber. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
XXXV Reunión Científica Anual de la Sociedad de Biología de Cuyo
Villa de Merlo
Argentina
Sociedad de Biología de Cuyo
Materia
ADRENAL GLAND
CHROMAFFIN CELLS
HISTOCHEMICAL STUDY
VISCACHA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/162752
Acceder
id CONICETDig_f3f599af785194d6a103bca3907b718f
oai_identifier_str oai:ri.conicet.gov.ar:11336/162752
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chromaffin and sustentacular cells in the adrenal medulla of the viscacha (Lagostomus maximus maximus): A histochemical and immunohistochemical studyGallol, Luis EzequielMohamed, Fabian HeberADRENAL GLANDCHROMAFFIN CELLSHISTOCHEMICAL STUDYVISCACHAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Chromaffin and sustentacular cells are common components of the adrenal medulla of mammalian species. However, subpopulations of these cells varies among the different species. While the rat adrenal medulla is known to contain noradrenergic chromaffin cells in close relation with GFAP (glial fibrillary acidic protein)-positive sustentacular cells (SC-GFAP) other species express only chromaffin cells with an adrenergic phenotype and lack of SC-GFAPir. The aim of this study is to assess the distribution of adrenergic and noradrenergic chromaffin cells and SC-GFAPir in the adrenal medulla of the viscacha. Four male viscachas (N=4) and four male rats (N=4; technique control) were used in this study. In order to study the presence of adrenergic and noradrenergic chromaffin cells in the adrenal medulla, we used the histochemical method described by Honoré. The presence of SC-GFAPir was study by immunohistochemistry using an antibody against GFAP. In our study, the histochemical detection of noradrenergic chromaffin cells was negative throughout the adrenal medulla of the viscacha suggesting an adrenergic phenotype for this species, while the rat adrenal medulla used as control showed a strong positive reaction. The immunohistochemical detection of SC-GFAPir was also negative in the viscacha. These results showed that the viscacha adrenal medulla contains only chromaffin cells with an adrenergic phenotype and lack of SCGFAPir. This study is in accordance and contributes with the current literature adding another observation that suggests that SC-GFAPir are only expressed in presence of chromaffin cells with a noradrenergic phenotype. However, further studies are necessary to confirm this relation.Fil: Gallol, Luis Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Mohamed, Fabian Heber. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaXXXV Reunión Científica Anual de la Sociedad de Biología de CuyoVilla de MerloArgentinaSociedad de Biología de CuyoSociedad de Biología de Cuyo2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/162752Chromaffin and sustentacular cells in the adrenal medulla of the viscacha (Lagostomus maximus maximus): A histochemical and immunohistochemical study; XXXV Reunión Científica Anual de la Sociedad de Biología de Cuyo; Villa de Merlo; Argentina; 2017; 31-31CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/reuniones-anuales-anteriores/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:06:08Zoai:ri.conicet.gov.ar:11336/162752instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:06:08.466CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chromaffin and sustentacular cells in the adrenal medulla of the viscacha (Lagostomus maximus maximus): A histochemical and immunohistochemical study
title Chromaffin and sustentacular cells in the adrenal medulla of the viscacha (Lagostomus maximus maximus): A histochemical and immunohistochemical study
spellingShingle Chromaffin and sustentacular cells in the adrenal medulla of the viscacha (Lagostomus maximus maximus): A histochemical and immunohistochemical study
Gallol, Luis Ezequiel
ADRENAL GLAND
CHROMAFFIN CELLS
HISTOCHEMICAL STUDY
VISCACHA
title_short Chromaffin and sustentacular cells in the adrenal medulla of the viscacha (Lagostomus maximus maximus): A histochemical and immunohistochemical study
title_full Chromaffin and sustentacular cells in the adrenal medulla of the viscacha (Lagostomus maximus maximus): A histochemical and immunohistochemical study
title_fullStr Chromaffin and sustentacular cells in the adrenal medulla of the viscacha (Lagostomus maximus maximus): A histochemical and immunohistochemical study
title_full_unstemmed Chromaffin and sustentacular cells in the adrenal medulla of the viscacha (Lagostomus maximus maximus): A histochemical and immunohistochemical study
title_sort Chromaffin and sustentacular cells in the adrenal medulla of the viscacha (Lagostomus maximus maximus): A histochemical and immunohistochemical study
dc.creator.none.fl_str_mv Gallol, Luis Ezequiel
Mohamed, Fabian Heber
author Gallol, Luis Ezequiel
author_facet Gallol, Luis Ezequiel
Mohamed, Fabian Heber
author_role author
author2 Mohamed, Fabian Heber
author2_role author
dc.subject.none.fl_str_mv ADRENAL GLAND
CHROMAFFIN CELLS
HISTOCHEMICAL STUDY
VISCACHA
topic ADRENAL GLAND
CHROMAFFIN CELLS
HISTOCHEMICAL STUDY
VISCACHA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Chromaffin and sustentacular cells are common components of the adrenal medulla of mammalian species. However, subpopulations of these cells varies among the different species. While the rat adrenal medulla is known to contain noradrenergic chromaffin cells in close relation with GFAP (glial fibrillary acidic protein)-positive sustentacular cells (SC-GFAP) other species express only chromaffin cells with an adrenergic phenotype and lack of SC-GFAPir. The aim of this study is to assess the distribution of adrenergic and noradrenergic chromaffin cells and SC-GFAPir in the adrenal medulla of the viscacha. Four male viscachas (N=4) and four male rats (N=4; technique control) were used in this study. In order to study the presence of adrenergic and noradrenergic chromaffin cells in the adrenal medulla, we used the histochemical method described by Honoré. The presence of SC-GFAPir was study by immunohistochemistry using an antibody against GFAP. In our study, the histochemical detection of noradrenergic chromaffin cells was negative throughout the adrenal medulla of the viscacha suggesting an adrenergic phenotype for this species, while the rat adrenal medulla used as control showed a strong positive reaction. The immunohistochemical detection of SC-GFAPir was also negative in the viscacha. These results showed that the viscacha adrenal medulla contains only chromaffin cells with an adrenergic phenotype and lack of SCGFAPir. This study is in accordance and contributes with the current literature adding another observation that suggests that SC-GFAPir are only expressed in presence of chromaffin cells with a noradrenergic phenotype. However, further studies are necessary to confirm this relation.
Fil: Gallol, Luis Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Mohamed, Fabian Heber. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
XXXV Reunión Científica Anual de la Sociedad de Biología de Cuyo
Villa de Merlo
Argentina
Sociedad de Biología de Cuyo
description Chromaffin and sustentacular cells are common components of the adrenal medulla of mammalian species. However, subpopulations of these cells varies among the different species. While the rat adrenal medulla is known to contain noradrenergic chromaffin cells in close relation with GFAP (glial fibrillary acidic protein)-positive sustentacular cells (SC-GFAP) other species express only chromaffin cells with an adrenergic phenotype and lack of SC-GFAPir. The aim of this study is to assess the distribution of adrenergic and noradrenergic chromaffin cells and SC-GFAPir in the adrenal medulla of the viscacha. Four male viscachas (N=4) and four male rats (N=4; technique control) were used in this study. In order to study the presence of adrenergic and noradrenergic chromaffin cells in the adrenal medulla, we used the histochemical method described by Honoré. The presence of SC-GFAPir was study by immunohistochemistry using an antibody against GFAP. In our study, the histochemical detection of noradrenergic chromaffin cells was negative throughout the adrenal medulla of the viscacha suggesting an adrenergic phenotype for this species, while the rat adrenal medulla used as control showed a strong positive reaction. The immunohistochemical detection of SC-GFAPir was also negative in the viscacha. These results showed that the viscacha adrenal medulla contains only chromaffin cells with an adrenergic phenotype and lack of SCGFAPir. This study is in accordance and contributes with the current literature adding another observation that suggests that SC-GFAPir are only expressed in presence of chromaffin cells with a noradrenergic phenotype. However, further studies are necessary to confirm this relation.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/162752
Chromaffin and sustentacular cells in the adrenal medulla of the viscacha (Lagostomus maximus maximus): A histochemical and immunohistochemical study; XXXV Reunión Científica Anual de la Sociedad de Biología de Cuyo; Villa de Merlo; Argentina; 2017; 31-31
CONICET Digital
CONICET
url http://hdl.handle.net/11336/162752
identifier_str_mv Chromaffin and sustentacular cells in the adrenal medulla of the viscacha (Lagostomus maximus maximus): A histochemical and immunohistochemical study; XXXV Reunión Científica Anual de la Sociedad de Biología de Cuyo; Villa de Merlo; Argentina; 2017; 31-31
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/reuniones-anuales-anteriores/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad de Biología de Cuyo
publisher.none.fl_str_mv Sociedad de Biología de Cuyo
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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