Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence
- Autores
- Baptista, Juliana Cristina; Machado, Marcos Antonio; Homem, Rafael Augusto; Torres, Pablo Sebastian; Vojnov, Adrián Alberto; do Amaral, Alexandre Morais
- Año de publicación
- 2010
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The Gram-negative bacterium Xanthomonas axonopodispv. citri, the causal agent of citrus canker, is a major threat to the citrus industry worldwide. Although this is a leaf spot pathogen, it bears genes highly related to degradation of plant cell walls, which are typically found in plant pathogens that cause symptoms of tissue maceration. Little is known on Xaccapacity to cause disease and hydrolyze cellulose. We investigated the contribution of various open reading frames on degradation of a cellulose compound by means of a global mutational assay to selectively screen for a defect in carboxymethyl cellulase (CMCase) secretion in X. axonopodispv. citri. Screening on CMC agar revealed one mutant clone defective in extracellular glycanase activity, out of nearly 3,000 clones. The insertion was located in the xpsDgene, a component of the type II secretion system (T2SS) showing an influence in the ability of Xac to colonize tissues and hydrolyze cellulose. In summary, these data show for the first time, that X. axonopodispv. citri is capable of hydrolyzing cellulose in a T2SS-dependent process. Furthermore, it was demonstrated that the ability to degrade cellulose contributes to the infection process as a whole.
Fil: Baptista, Juliana Cristina. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; Brasil. Universidade Estadual de Campinas; Brasil
Fil: Machado, Marcos Antonio. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; Brasil
Fil: Homem, Rafael Augusto. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; Brasil. Universidade Estadual de Campinas; Brasil
Fil: Torres, Pablo Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina
Fil: Vojnov, Adrián Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina
Fil: do Amaral, Alexandre Morais. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; Brasil. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil - Materia
-
CITRUS CANKER
TRANSPOSOME
TYPE II SECRETION SYSTEM - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/69112
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Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulenceBaptista, Juliana CristinaMachado, Marcos AntonioHomem, Rafael AugustoTorres, Pablo SebastianVojnov, Adrián Albertodo Amaral, Alexandre MoraisCITRUS CANKERTRANSPOSOMETYPE II SECRETION SYSTEMhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4The Gram-negative bacterium Xanthomonas axonopodispv. citri, the causal agent of citrus canker, is a major threat to the citrus industry worldwide. Although this is a leaf spot pathogen, it bears genes highly related to degradation of plant cell walls, which are typically found in plant pathogens that cause symptoms of tissue maceration. Little is known on Xaccapacity to cause disease and hydrolyze cellulose. We investigated the contribution of various open reading frames on degradation of a cellulose compound by means of a global mutational assay to selectively screen for a defect in carboxymethyl cellulase (CMCase) secretion in X. axonopodispv. citri. Screening on CMC agar revealed one mutant clone defective in extracellular glycanase activity, out of nearly 3,000 clones. The insertion was located in the xpsDgene, a component of the type II secretion system (T2SS) showing an influence in the ability of Xac to colonize tissues and hydrolyze cellulose. In summary, these data show for the first time, that X. axonopodispv. citri is capable of hydrolyzing cellulose in a T2SS-dependent process. Furthermore, it was demonstrated that the ability to degrade cellulose contributes to the infection process as a whole.Fil: Baptista, Juliana Cristina. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; Brasil. Universidade Estadual de Campinas; BrasilFil: Machado, Marcos Antonio. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; BrasilFil: Homem, Rafael Augusto. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; Brasil. Universidade Estadual de Campinas; BrasilFil: Torres, Pablo Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; ArgentinaFil: Vojnov, Adrián Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; ArgentinaFil: do Amaral, Alexandre Morais. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; Brasil. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; BrasilSociedade Brasileira de Genética2010-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/69112Baptista, Juliana Cristina; Machado, Marcos Antonio; Homem, Rafael Augusto; Torres, Pablo Sebastian; Vojnov, Adrián Alberto; et al.; Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence; Sociedade Brasileira de Genética; Genetics and Molecular Biology; 33; 1; 3-2010; 146-1531415-47571678-4685CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1590/S1415-47572009005000110info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/z5c56jinfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3036071/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:35:16Zoai:ri.conicet.gov.ar:11336/69112instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:35:16.925CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence |
title |
Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence |
spellingShingle |
Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence Baptista, Juliana Cristina CITRUS CANKER TRANSPOSOME TYPE II SECRETION SYSTEM |
title_short |
Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence |
title_full |
Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence |
title_fullStr |
Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence |
title_full_unstemmed |
Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence |
title_sort |
Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence |
dc.creator.none.fl_str_mv |
Baptista, Juliana Cristina Machado, Marcos Antonio Homem, Rafael Augusto Torres, Pablo Sebastian Vojnov, Adrián Alberto do Amaral, Alexandre Morais |
author |
Baptista, Juliana Cristina |
author_facet |
Baptista, Juliana Cristina Machado, Marcos Antonio Homem, Rafael Augusto Torres, Pablo Sebastian Vojnov, Adrián Alberto do Amaral, Alexandre Morais |
author_role |
author |
author2 |
Machado, Marcos Antonio Homem, Rafael Augusto Torres, Pablo Sebastian Vojnov, Adrián Alberto do Amaral, Alexandre Morais |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
CITRUS CANKER TRANSPOSOME TYPE II SECRETION SYSTEM |
topic |
CITRUS CANKER TRANSPOSOME TYPE II SECRETION SYSTEM |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.1 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
The Gram-negative bacterium Xanthomonas axonopodispv. citri, the causal agent of citrus canker, is a major threat to the citrus industry worldwide. Although this is a leaf spot pathogen, it bears genes highly related to degradation of plant cell walls, which are typically found in plant pathogens that cause symptoms of tissue maceration. Little is known on Xaccapacity to cause disease and hydrolyze cellulose. We investigated the contribution of various open reading frames on degradation of a cellulose compound by means of a global mutational assay to selectively screen for a defect in carboxymethyl cellulase (CMCase) secretion in X. axonopodispv. citri. Screening on CMC agar revealed one mutant clone defective in extracellular glycanase activity, out of nearly 3,000 clones. The insertion was located in the xpsDgene, a component of the type II secretion system (T2SS) showing an influence in the ability of Xac to colonize tissues and hydrolyze cellulose. In summary, these data show for the first time, that X. axonopodispv. citri is capable of hydrolyzing cellulose in a T2SS-dependent process. Furthermore, it was demonstrated that the ability to degrade cellulose contributes to the infection process as a whole. Fil: Baptista, Juliana Cristina. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; Brasil. Universidade Estadual de Campinas; Brasil Fil: Machado, Marcos Antonio. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; Brasil Fil: Homem, Rafael Augusto. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; Brasil. Universidade Estadual de Campinas; Brasil Fil: Torres, Pablo Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina Fil: Vojnov, Adrián Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina Fil: do Amaral, Alexandre Morais. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; Brasil. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil |
description |
The Gram-negative bacterium Xanthomonas axonopodispv. citri, the causal agent of citrus canker, is a major threat to the citrus industry worldwide. Although this is a leaf spot pathogen, it bears genes highly related to degradation of plant cell walls, which are typically found in plant pathogens that cause symptoms of tissue maceration. Little is known on Xaccapacity to cause disease and hydrolyze cellulose. We investigated the contribution of various open reading frames on degradation of a cellulose compound by means of a global mutational assay to selectively screen for a defect in carboxymethyl cellulase (CMCase) secretion in X. axonopodispv. citri. Screening on CMC agar revealed one mutant clone defective in extracellular glycanase activity, out of nearly 3,000 clones. The insertion was located in the xpsDgene, a component of the type II secretion system (T2SS) showing an influence in the ability of Xac to colonize tissues and hydrolyze cellulose. In summary, these data show for the first time, that X. axonopodispv. citri is capable of hydrolyzing cellulose in a T2SS-dependent process. Furthermore, it was demonstrated that the ability to degrade cellulose contributes to the infection process as a whole. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/69112 Baptista, Juliana Cristina; Machado, Marcos Antonio; Homem, Rafael Augusto; Torres, Pablo Sebastian; Vojnov, Adrián Alberto; et al.; Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence; Sociedade Brasileira de Genética; Genetics and Molecular Biology; 33; 1; 3-2010; 146-153 1415-4757 1678-4685 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/69112 |
identifier_str_mv |
Baptista, Juliana Cristina; Machado, Marcos Antonio; Homem, Rafael Augusto; Torres, Pablo Sebastian; Vojnov, Adrián Alberto; et al.; Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence; Sociedade Brasileira de Genética; Genetics and Molecular Biology; 33; 1; 3-2010; 146-153 1415-4757 1678-4685 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1590/S1415-47572009005000110 info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/z5c56j info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3036071/ |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |