Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence

Autores
Baptista, Juliana Cristina; Machado, Marcos Antonio; Homem, Rafael Augusto; Torres, Pablo Sebastian; Vojnov, Adrián Alberto; do Amaral, Alexandre Morais
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Gram-negative bacterium Xanthomonas axonopodispv. citri, the causal agent of citrus canker, is a major threat to the citrus industry worldwide. Although this is a leaf spot pathogen, it bears genes highly related to degradation of plant cell walls, which are typically found in plant pathogens that cause symptoms of tissue maceration. Little is known on Xaccapacity to cause disease and hydrolyze cellulose. We investigated the contribution of various open reading frames on degradation of a cellulose compound by means of a global mutational assay to selectively screen for a defect in carboxymethyl cellulase (CMCase) secretion in X. axonopodispv. citri. Screening on CMC agar revealed one mutant clone defective in extracellular glycanase activity, out of nearly 3,000 clones. The insertion was located in the xpsDgene, a component of the type II secretion system (T2SS) showing an influence in the ability of Xac to colonize tissues and hydrolyze cellulose. In summary, these data show for the first time, that X. axonopodispv. citri is capable of hydrolyzing cellulose in a T2SS-dependent process. Furthermore, it was demonstrated that the ability to degrade cellulose contributes to the infection process as a whole.
Fil: Baptista, Juliana Cristina. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; Brasil. Universidade Estadual de Campinas; Brasil
Fil: Machado, Marcos Antonio. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; Brasil
Fil: Homem, Rafael Augusto. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; Brasil. Universidade Estadual de Campinas; Brasil
Fil: Torres, Pablo Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina
Fil: Vojnov, Adrián Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina
Fil: do Amaral, Alexandre Morais. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; Brasil. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Materia
CITRUS CANKER
TRANSPOSOME
TYPE II SECRETION SYSTEM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/69112
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/69112
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulenceBaptista, Juliana CristinaMachado, Marcos AntonioHomem, Rafael AugustoTorres, Pablo SebastianVojnov, Adrián Albertodo Amaral, Alexandre MoraisCITRUS CANKERTRANSPOSOMETYPE II SECRETION SYSTEMhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4The Gram-negative bacterium Xanthomonas axonopodispv. citri, the causal agent of citrus canker, is a major threat to the citrus industry worldwide. Although this is a leaf spot pathogen, it bears genes highly related to degradation of plant cell walls, which are typically found in plant pathogens that cause symptoms of tissue maceration. Little is known on Xaccapacity to cause disease and hydrolyze cellulose. We investigated the contribution of various open reading frames on degradation of a cellulose compound by means of a global mutational assay to selectively screen for a defect in carboxymethyl cellulase (CMCase) secretion in X. axonopodispv. citri. Screening on CMC agar revealed one mutant clone defective in extracellular glycanase activity, out of nearly 3,000 clones. The insertion was located in the xpsDgene, a component of the type II secretion system (T2SS) showing an influence in the ability of Xac to colonize tissues and hydrolyze cellulose. In summary, these data show for the first time, that X. axonopodispv. citri is capable of hydrolyzing cellulose in a T2SS-dependent process. Furthermore, it was demonstrated that the ability to degrade cellulose contributes to the infection process as a whole.Fil: Baptista, Juliana Cristina. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; Brasil. Universidade Estadual de Campinas; BrasilFil: Machado, Marcos Antonio. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; BrasilFil: Homem, Rafael Augusto. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; Brasil. Universidade Estadual de Campinas; BrasilFil: Torres, Pablo Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; ArgentinaFil: Vojnov, Adrián Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; ArgentinaFil: do Amaral, Alexandre Morais. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; Brasil. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; BrasilSociedade Brasileira de Genética2010-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/69112Baptista, Juliana Cristina; Machado, Marcos Antonio; Homem, Rafael Augusto; Torres, Pablo Sebastian; Vojnov, Adrián Alberto; et al.; Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence; Sociedade Brasileira de Genética; Genetics and Molecular Biology; 33; 1; 3-2010; 146-1531415-47571678-4685CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1590/S1415-47572009005000110info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/z5c56jinfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3036071/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:32:48Zoai:ri.conicet.gov.ar:11336/69112instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:32:48.329CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence
title Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence
spellingShingle Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence
Baptista, Juliana Cristina
CITRUS CANKER
TRANSPOSOME
TYPE II SECRETION SYSTEM
title_short Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence
title_full Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence
title_fullStr Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence
title_full_unstemmed Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence
title_sort Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence
dc.creator.none.fl_str_mv Baptista, Juliana Cristina
Machado, Marcos Antonio
Homem, Rafael Augusto
Torres, Pablo Sebastian
Vojnov, Adrián Alberto
do Amaral, Alexandre Morais
author Baptista, Juliana Cristina
author_facet Baptista, Juliana Cristina
Machado, Marcos Antonio
Homem, Rafael Augusto
Torres, Pablo Sebastian
Vojnov, Adrián Alberto
do Amaral, Alexandre Morais
author_role author
author2 Machado, Marcos Antonio
Homem, Rafael Augusto
Torres, Pablo Sebastian
Vojnov, Adrián Alberto
do Amaral, Alexandre Morais
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CITRUS CANKER
TRANSPOSOME
TYPE II SECRETION SYSTEM
topic CITRUS CANKER
TRANSPOSOME
TYPE II SECRETION SYSTEM
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The Gram-negative bacterium Xanthomonas axonopodispv. citri, the causal agent of citrus canker, is a major threat to the citrus industry worldwide. Although this is a leaf spot pathogen, it bears genes highly related to degradation of plant cell walls, which are typically found in plant pathogens that cause symptoms of tissue maceration. Little is known on Xaccapacity to cause disease and hydrolyze cellulose. We investigated the contribution of various open reading frames on degradation of a cellulose compound by means of a global mutational assay to selectively screen for a defect in carboxymethyl cellulase (CMCase) secretion in X. axonopodispv. citri. Screening on CMC agar revealed one mutant clone defective in extracellular glycanase activity, out of nearly 3,000 clones. The insertion was located in the xpsDgene, a component of the type II secretion system (T2SS) showing an influence in the ability of Xac to colonize tissues and hydrolyze cellulose. In summary, these data show for the first time, that X. axonopodispv. citri is capable of hydrolyzing cellulose in a T2SS-dependent process. Furthermore, it was demonstrated that the ability to degrade cellulose contributes to the infection process as a whole.
Fil: Baptista, Juliana Cristina. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; Brasil. Universidade Estadual de Campinas; Brasil
Fil: Machado, Marcos Antonio. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; Brasil
Fil: Homem, Rafael Augusto. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; Brasil. Universidade Estadual de Campinas; Brasil
Fil: Torres, Pablo Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina
Fil: Vojnov, Adrián Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina
Fil: do Amaral, Alexandre Morais. Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira ; Brasil. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
description The Gram-negative bacterium Xanthomonas axonopodispv. citri, the causal agent of citrus canker, is a major threat to the citrus industry worldwide. Although this is a leaf spot pathogen, it bears genes highly related to degradation of plant cell walls, which are typically found in plant pathogens that cause symptoms of tissue maceration. Little is known on Xaccapacity to cause disease and hydrolyze cellulose. We investigated the contribution of various open reading frames on degradation of a cellulose compound by means of a global mutational assay to selectively screen for a defect in carboxymethyl cellulase (CMCase) secretion in X. axonopodispv. citri. Screening on CMC agar revealed one mutant clone defective in extracellular glycanase activity, out of nearly 3,000 clones. The insertion was located in the xpsDgene, a component of the type II secretion system (T2SS) showing an influence in the ability of Xac to colonize tissues and hydrolyze cellulose. In summary, these data show for the first time, that X. axonopodispv. citri is capable of hydrolyzing cellulose in a T2SS-dependent process. Furthermore, it was demonstrated that the ability to degrade cellulose contributes to the infection process as a whole.
publishDate 2010
dc.date.none.fl_str_mv 2010-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/69112
Baptista, Juliana Cristina; Machado, Marcos Antonio; Homem, Rafael Augusto; Torres, Pablo Sebastian; Vojnov, Adrián Alberto; et al.; Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence; Sociedade Brasileira de Genética; Genetics and Molecular Biology; 33; 1; 3-2010; 146-153
1415-4757
1678-4685
CONICET Digital
CONICET
url http://hdl.handle.net/11336/69112
identifier_str_mv Baptista, Juliana Cristina; Machado, Marcos Antonio; Homem, Rafael Augusto; Torres, Pablo Sebastian; Vojnov, Adrián Alberto; et al.; Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence; Sociedade Brasileira de Genética; Genetics and Molecular Biology; 33; 1; 3-2010; 146-153
1415-4757
1678-4685
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1590/S1415-47572009005000110
info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/z5c56j
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3036071/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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