Differential expression of Glycerol-3-Phosphate Dehydrogenase isoforms in flight muscles of the Chagas disease vector Triatoma infestans (Hemiptera, Reduviidae)

Autores: Stroppa, Maria Mercedes; Lagunas, Mariana Stefania; Carriazo, Carlota; Garcia, Beatriz Alicia; Iraola, Gregorio; Panzera, Yanina; Gerez de Burgos, Nelia Martha
Año de publicación: 2013
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Flight muscles of Triatoma infestans have two glycerol-3-phosphate dehydrogenase (GPDH) isoforms: GPDH-1 is involved in flight metabolism and GPDH-2 provides lipid precursors. Total GPDH activity was greater in the natural population and almost only due to GPDH-1. Different expression and activity observed between GPDH isoforms in the natural population and the first laboratory generation was not detected in the second laboratory generation. This pattern may be caused by gradual adaptation to laboratory nutritional conditions. During development, the expression of GPDH-2 increased with a longer time of intake, which would imply an increment in lipid biosynthesis. The GPDH-1 transcript predominated with respect to that of GPDH-2 in the lower nutritional condition, suggesting the necessity of insects to fly during this nutritional status. The transcriptional pattern showed a delay at 22°C. The isoforms activities and transcript patterns in flight muscles suggest transcriptional adaptation to metabolic requirements originated by alternative splicing.
Fil: Stroppa, Maria Mercedes. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina. Universidad de la Republica; Uruguay
Fil: Lagunas, Mariana Stefania. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina. Universidad de la Republica; Uruguay
Fil: Carriazo, Carlota. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina. Universidad de la Republica; Uruguay
Fil: Garcia, Beatriz Alicia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina. Universidad de la Republica; Uruguay
Fil: Iraola, Gregorio. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina. Universidad de la Republica; Uruguay
Fil: Panzera, Yanina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina. Universidad de la Republica; Uruguay
Fil: Gerez de Burgos, Nelia Martha. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina. Universidad de la Republica; Uruguay
Materia: Triatoma
Chagas
Gpdh
Expression
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/11801

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network_name_str	CONICET Digital (CONICET)
spelling	Differential expression of Glycerol-3-Phosphate Dehydrogenase isoforms in flight muscles of the Chagas disease vector Triatoma infestans (Hemiptera, Reduviidae)Stroppa, Maria MercedesLagunas, Mariana StefaniaCarriazo, CarlotaGarcia, Beatriz AliciaIraola, GregorioPanzera, YaninaGerez de Burgos, Nelia MarthaTriatomaChagasGpdhExpressionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Flight muscles of Triatoma infestans have two glycerol-3-phosphate dehydrogenase (GPDH) isoforms: GPDH-1 is involved in flight metabolism and GPDH-2 provides lipid precursors. Total GPDH activity was greater in the natural population and almost only due to GPDH-1. Different expression and activity observed between GPDH isoforms in the natural population and the first laboratory generation was not detected in the second laboratory generation. This pattern may be caused by gradual adaptation to laboratory nutritional conditions. During development, the expression of GPDH-2 increased with a longer time of intake, which would imply an increment in lipid biosynthesis. The GPDH-1 transcript predominated with respect to that of GPDH-2 in the lower nutritional condition, suggesting the necessity of insects to fly during this nutritional status. The transcriptional pattern showed a delay at 22°C. The isoforms activities and transcript patterns in flight muscles suggest transcriptional adaptation to metabolic requirements originated by alternative splicing.Fil: Stroppa, Maria Mercedes. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina. Universidad de la Republica; UruguayFil: Lagunas, Mariana Stefania. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina. Universidad de la Republica; UruguayFil: Carriazo, Carlota. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina. Universidad de la Republica; UruguayFil: Garcia, Beatriz Alicia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina. Universidad de la Republica; UruguayFil: Iraola, Gregorio. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina. Universidad de la Republica; UruguayFil: Panzera, Yanina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina. Universidad de la Republica; UruguayFil: Gerez de Burgos, Nelia Martha. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina. Universidad de la Republica; UruguayAmerican Society Of Tropical Medicine And Hygiene2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11801Stroppa, Maria Mercedes; Lagunas, Mariana Stefania; Carriazo, Carlota; Garcia, Beatriz Alicia; Iraola, Gregorio; et al.; Differential expression of Glycerol-3-Phosphate Dehydrogenase isoforms in flight muscles of the Chagas disease vector Triatoma infestans (Hemiptera, Reduviidae); American Society Of Tropical Medicine And Hygiene; American Journal Of Tropical Medicine And Hygiene; 88; 6; 3-2013; 1146–11510002-9637enginfo:eu-repo/semantics/altIdentifier/doi/10.4269/ajtmh.12-0464info:eu-repo/semantics/altIdentifier/url/http://www.ajtmh.org/content/88/6/1146info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:21Zoai:ri.conicet.gov.ar:11336/11801instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:21.642CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Differential expression of Glycerol-3-Phosphate Dehydrogenase isoforms in flight muscles of the Chagas disease vector Triatoma infestans (Hemiptera, Reduviidae)
title	Differential expression of Glycerol-3-Phosphate Dehydrogenase isoforms in flight muscles of the Chagas disease vector Triatoma infestans (Hemiptera, Reduviidae)
spellingShingle	Differential expression of Glycerol-3-Phosphate Dehydrogenase isoforms in flight muscles of the Chagas disease vector Triatoma infestans (Hemiptera, Reduviidae) Stroppa, Maria Mercedes Triatoma Chagas Gpdh Expression
title_short	Differential expression of Glycerol-3-Phosphate Dehydrogenase isoforms in flight muscles of the Chagas disease vector Triatoma infestans (Hemiptera, Reduviidae)
title_full	Differential expression of Glycerol-3-Phosphate Dehydrogenase isoforms in flight muscles of the Chagas disease vector Triatoma infestans (Hemiptera, Reduviidae)
title_fullStr	Differential expression of Glycerol-3-Phosphate Dehydrogenase isoforms in flight muscles of the Chagas disease vector Triatoma infestans (Hemiptera, Reduviidae)
title_full_unstemmed	Differential expression of Glycerol-3-Phosphate Dehydrogenase isoforms in flight muscles of the Chagas disease vector Triatoma infestans (Hemiptera, Reduviidae)
title_sort	Differential expression of Glycerol-3-Phosphate Dehydrogenase isoforms in flight muscles of the Chagas disease vector Triatoma infestans (Hemiptera, Reduviidae)
dc.creator.none.fl_str_mv	Stroppa, Maria Mercedes Lagunas, Mariana Stefania Carriazo, Carlota Garcia, Beatriz Alicia Iraola, Gregorio Panzera, Yanina Gerez de Burgos, Nelia Martha
author	Stroppa, Maria Mercedes
author_facet	Stroppa, Maria Mercedes Lagunas, Mariana Stefania Carriazo, Carlota Garcia, Beatriz Alicia Iraola, Gregorio Panzera, Yanina Gerez de Burgos, Nelia Martha
author_role	author
author2	Lagunas, Mariana Stefania Carriazo, Carlota Garcia, Beatriz Alicia Iraola, Gregorio Panzera, Yanina Gerez de Burgos, Nelia Martha
author2_role	author author author author author author
dc.subject.none.fl_str_mv	Triatoma Chagas Gpdh Expression
topic	Triatoma Chagas Gpdh Expression
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv	Flight muscles of Triatoma infestans have two glycerol-3-phosphate dehydrogenase (GPDH) isoforms: GPDH-1 is involved in flight metabolism and GPDH-2 provides lipid precursors. Total GPDH activity was greater in the natural population and almost only due to GPDH-1. Different expression and activity observed between GPDH isoforms in the natural population and the first laboratory generation was not detected in the second laboratory generation. This pattern may be caused by gradual adaptation to laboratory nutritional conditions. During development, the expression of GPDH-2 increased with a longer time of intake, which would imply an increment in lipid biosynthesis. The GPDH-1 transcript predominated with respect to that of GPDH-2 in the lower nutritional condition, suggesting the necessity of insects to fly during this nutritional status. The transcriptional pattern showed a delay at 22°C. The isoforms activities and transcript patterns in flight muscles suggest transcriptional adaptation to metabolic requirements originated by alternative splicing. Fil: Stroppa, Maria Mercedes. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina. Universidad de la Republica; Uruguay Fil: Lagunas, Mariana Stefania. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina. Universidad de la Republica; Uruguay Fil: Carriazo, Carlota. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina. Universidad de la Republica; Uruguay Fil: Garcia, Beatriz Alicia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina. Universidad de la Republica; Uruguay Fil: Iraola, Gregorio. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina. Universidad de la Republica; Uruguay Fil: Panzera, Yanina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina. Universidad de la Republica; Uruguay Fil: Gerez de Burgos, Nelia Martha. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina. Universidad de la Republica; Uruguay
description	Flight muscles of Triatoma infestans have two glycerol-3-phosphate dehydrogenase (GPDH) isoforms: GPDH-1 is involved in flight metabolism and GPDH-2 provides lipid precursors. Total GPDH activity was greater in the natural population and almost only due to GPDH-1. Different expression and activity observed between GPDH isoforms in the natural population and the first laboratory generation was not detected in the second laboratory generation. This pattern may be caused by gradual adaptation to laboratory nutritional conditions. During development, the expression of GPDH-2 increased with a longer time of intake, which would imply an increment in lipid biosynthesis. The GPDH-1 transcript predominated with respect to that of GPDH-2 in the lower nutritional condition, suggesting the necessity of insects to fly during this nutritional status. The transcriptional pattern showed a delay at 22°C. The isoforms activities and transcript patterns in flight muscles suggest transcriptional adaptation to metabolic requirements originated by alternative splicing.
publishDate	2013
dc.date.none.fl_str_mv	2013-03
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/11801 Stroppa, Maria Mercedes; Lagunas, Mariana Stefania; Carriazo, Carlota; Garcia, Beatriz Alicia; Iraola, Gregorio; et al.; Differential expression of Glycerol-3-Phosphate Dehydrogenase isoforms in flight muscles of the Chagas disease vector Triatoma infestans (Hemiptera, Reduviidae); American Society Of Tropical Medicine And Hygiene; American Journal Of Tropical Medicine And Hygiene; 88; 6; 3-2013; 1146–1151 0002-9637
url	http://hdl.handle.net/11336/11801
identifier_str_mv	Stroppa, Maria Mercedes; Lagunas, Mariana Stefania; Carriazo, Carlota; Garcia, Beatriz Alicia; Iraola, Gregorio; et al.; Differential expression of Glycerol-3-Phosphate Dehydrogenase isoforms in flight muscles of the Chagas disease vector Triatoma infestans (Hemiptera, Reduviidae); American Society Of Tropical Medicine And Hygiene; American Journal Of Tropical Medicine And Hygiene; 88; 6; 3-2013; 1146–1151 0002-9637
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/doi/10.4269/ajtmh.12-0464 info:eu-repo/semantics/altIdentifier/url/http://www.ajtmh.org/content/88/6/1146
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rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.publisher.none.fl_str_mv	American Society Of Tropical Medicine And Hygiene
publisher.none.fl_str_mv	American Society Of Tropical Medicine And Hygiene
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
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repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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Differential expression of Glycerol-3-Phosphate Dehydrogenase isoforms in flight muscles of the Chagas disease vector Triatoma infestans (Hemiptera, Reduviidae)

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