Antagonistic Effects of Corynebacterium pseudodiphtheriticum 090104 on Respiratory Pathogens

Autores
Ortiz Moyano, Francisco Ramiro; Dentice Maidana, Stefania; Imamura, Yoshiya; Elean, Mariano Daniel; Namai, Fu; Suda, Yoshihito; Nishiyama, Keita; Melnikov, Vyacheslav; Kitazawa, Haruki; Villena, Julio Cesar
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In previous studies, it was demonstrated that Corynebacterium pseudodiphtheriticum 090104, isolated from the human nasopharynx, modulates respiratory immunity, improving protection against infections. Here, the antagonistic effect of the 090104 strain on respiratory pathogens, including Streptococcus pneumoniae, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii, was explored. In a series of in vitro studies, the capacity of C. pseudodiphtheriticum 090104, its bacterium-like particles, and its culture supernatants to coaggregate, inhibit the growth, and change the virulent phenotype of pathogenic bacteria was evaluated. The results showed that the 090104 strain was able to exert a bacteriostatic effect on K. pneumoniae and S. pneumoniae growth. In addition, C. pseudodiphtheriticum 090104 coaggregated, inhibited biofilm formation, and induced phenotypic changes in all the respiratory pathogens evaluated. In conclusion, this work demonstrated that, in addition to its beneficial effects exerted by host–microbe interactions, C. pseudodiphtheriticum 090104 can enhance protection against respiratory pathogens through its microbe–microbe interactions. The mechanisms involved in such interactions should be evaluated in future research.
Fil: Ortiz Moyano, Francisco Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Dentice Maidana, Stefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Imamura, Yoshiya. Tohoku University; Japón
Fil: Elean, Mariano Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Namai, Fu. Tohoku University; Japón
Fil: Suda, Yoshihito. Miyagi University; Japón
Fil: Nishiyama, Keita. Tohoku University; Japón
Fil: Melnikov, Vyacheslav. Gabrichevsky Research Institute for Epidemiology and Microbiology; Rusia
Fil: Kitazawa, Haruki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
CORYNEBACTERIUM PSEUDODIPHTHERITICUM
RESPIRATORY PATHOGENS
MICROBE–MICROBE INTERACTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/260793
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/260793
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antagonistic Effects of Corynebacterium pseudodiphtheriticum 090104 on Respiratory PathogensOrtiz Moyano, Francisco RamiroDentice Maidana, StefaniaImamura, YoshiyaElean, Mariano DanielNamai, FuSuda, YoshihitoNishiyama, KeitaMelnikov, VyacheslavKitazawa, HarukiVillena, Julio CesarCORYNEBACTERIUM PSEUDODIPHTHERITICUMRESPIRATORY PATHOGENSMICROBE–MICROBE INTERACTIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3In previous studies, it was demonstrated that Corynebacterium pseudodiphtheriticum 090104, isolated from the human nasopharynx, modulates respiratory immunity, improving protection against infections. Here, the antagonistic effect of the 090104 strain on respiratory pathogens, including Streptococcus pneumoniae, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii, was explored. In a series of in vitro studies, the capacity of C. pseudodiphtheriticum 090104, its bacterium-like particles, and its culture supernatants to coaggregate, inhibit the growth, and change the virulent phenotype of pathogenic bacteria was evaluated. The results showed that the 090104 strain was able to exert a bacteriostatic effect on K. pneumoniae and S. pneumoniae growth. In addition, C. pseudodiphtheriticum 090104 coaggregated, inhibited biofilm formation, and induced phenotypic changes in all the respiratory pathogens evaluated. In conclusion, this work demonstrated that, in addition to its beneficial effects exerted by host–microbe interactions, C. pseudodiphtheriticum 090104 can enhance protection against respiratory pathogens through its microbe–microbe interactions. The mechanisms involved in such interactions should be evaluated in future research.Fil: Ortiz Moyano, Francisco Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Dentice Maidana, Stefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Imamura, Yoshiya. Tohoku University; JapónFil: Elean, Mariano Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Namai, Fu. Tohoku University; JapónFil: Suda, Yoshihito. Miyagi University; JapónFil: Nishiyama, Keita. Tohoku University; JapónFil: Melnikov, Vyacheslav. Gabrichevsky Research Institute for Epidemiology and Microbiology; RusiaFil: Kitazawa, Haruki. Tohoku University; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaMultidisciplinary Digital Publishing Institute2024-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/260793Ortiz Moyano, Francisco Ramiro; Dentice Maidana, Stefania; Imamura, Yoshiya; Elean, Mariano Daniel; Namai, Fu; et al.; Antagonistic Effects of Corynebacterium pseudodiphtheriticum 090104 on Respiratory Pathogens; Multidisciplinary Digital Publishing Institute; Microorganisms; 12; 7; 6-2024; 1-172076-2607CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/12/7/1295info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms12071295info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:44:39Zoai:ri.conicet.gov.ar:11336/260793instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:44:40.172CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antagonistic Effects of Corynebacterium pseudodiphtheriticum 090104 on Respiratory Pathogens
title Antagonistic Effects of Corynebacterium pseudodiphtheriticum 090104 on Respiratory Pathogens
spellingShingle Antagonistic Effects of Corynebacterium pseudodiphtheriticum 090104 on Respiratory Pathogens
Ortiz Moyano, Francisco Ramiro
CORYNEBACTERIUM PSEUDODIPHTHERITICUM
RESPIRATORY PATHOGENS
MICROBE–MICROBE INTERACTION
title_short Antagonistic Effects of Corynebacterium pseudodiphtheriticum 090104 on Respiratory Pathogens
title_full Antagonistic Effects of Corynebacterium pseudodiphtheriticum 090104 on Respiratory Pathogens
title_fullStr Antagonistic Effects of Corynebacterium pseudodiphtheriticum 090104 on Respiratory Pathogens
title_full_unstemmed Antagonistic Effects of Corynebacterium pseudodiphtheriticum 090104 on Respiratory Pathogens
title_sort Antagonistic Effects of Corynebacterium pseudodiphtheriticum 090104 on Respiratory Pathogens
dc.creator.none.fl_str_mv Ortiz Moyano, Francisco Ramiro
Dentice Maidana, Stefania
Imamura, Yoshiya
Elean, Mariano Daniel
Namai, Fu
Suda, Yoshihito
Nishiyama, Keita
Melnikov, Vyacheslav
Kitazawa, Haruki
Villena, Julio Cesar
author Ortiz Moyano, Francisco Ramiro
author_facet Ortiz Moyano, Francisco Ramiro
Dentice Maidana, Stefania
Imamura, Yoshiya
Elean, Mariano Daniel
Namai, Fu
Suda, Yoshihito
Nishiyama, Keita
Melnikov, Vyacheslav
Kitazawa, Haruki
Villena, Julio Cesar
author_role author
author2 Dentice Maidana, Stefania
Imamura, Yoshiya
Elean, Mariano Daniel
Namai, Fu
Suda, Yoshihito
Nishiyama, Keita
Melnikov, Vyacheslav
Kitazawa, Haruki
Villena, Julio Cesar
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CORYNEBACTERIUM PSEUDODIPHTHERITICUM
RESPIRATORY PATHOGENS
MICROBE–MICROBE INTERACTION
topic CORYNEBACTERIUM PSEUDODIPHTHERITICUM
RESPIRATORY PATHOGENS
MICROBE–MICROBE INTERACTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In previous studies, it was demonstrated that Corynebacterium pseudodiphtheriticum 090104, isolated from the human nasopharynx, modulates respiratory immunity, improving protection against infections. Here, the antagonistic effect of the 090104 strain on respiratory pathogens, including Streptococcus pneumoniae, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii, was explored. In a series of in vitro studies, the capacity of C. pseudodiphtheriticum 090104, its bacterium-like particles, and its culture supernatants to coaggregate, inhibit the growth, and change the virulent phenotype of pathogenic bacteria was evaluated. The results showed that the 090104 strain was able to exert a bacteriostatic effect on K. pneumoniae and S. pneumoniae growth. In addition, C. pseudodiphtheriticum 090104 coaggregated, inhibited biofilm formation, and induced phenotypic changes in all the respiratory pathogens evaluated. In conclusion, this work demonstrated that, in addition to its beneficial effects exerted by host–microbe interactions, C. pseudodiphtheriticum 090104 can enhance protection against respiratory pathogens through its microbe–microbe interactions. The mechanisms involved in such interactions should be evaluated in future research.
Fil: Ortiz Moyano, Francisco Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Dentice Maidana, Stefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Imamura, Yoshiya. Tohoku University; Japón
Fil: Elean, Mariano Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Namai, Fu. Tohoku University; Japón
Fil: Suda, Yoshihito. Miyagi University; Japón
Fil: Nishiyama, Keita. Tohoku University; Japón
Fil: Melnikov, Vyacheslav. Gabrichevsky Research Institute for Epidemiology and Microbiology; Rusia
Fil: Kitazawa, Haruki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description In previous studies, it was demonstrated that Corynebacterium pseudodiphtheriticum 090104, isolated from the human nasopharynx, modulates respiratory immunity, improving protection against infections. Here, the antagonistic effect of the 090104 strain on respiratory pathogens, including Streptococcus pneumoniae, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii, was explored. In a series of in vitro studies, the capacity of C. pseudodiphtheriticum 090104, its bacterium-like particles, and its culture supernatants to coaggregate, inhibit the growth, and change the virulent phenotype of pathogenic bacteria was evaluated. The results showed that the 090104 strain was able to exert a bacteriostatic effect on K. pneumoniae and S. pneumoniae growth. In addition, C. pseudodiphtheriticum 090104 coaggregated, inhibited biofilm formation, and induced phenotypic changes in all the respiratory pathogens evaluated. In conclusion, this work demonstrated that, in addition to its beneficial effects exerted by host–microbe interactions, C. pseudodiphtheriticum 090104 can enhance protection against respiratory pathogens through its microbe–microbe interactions. The mechanisms involved in such interactions should be evaluated in future research.
publishDate 2024
dc.date.none.fl_str_mv 2024-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/260793
Ortiz Moyano, Francisco Ramiro; Dentice Maidana, Stefania; Imamura, Yoshiya; Elean, Mariano Daniel; Namai, Fu; et al.; Antagonistic Effects of Corynebacterium pseudodiphtheriticum 090104 on Respiratory Pathogens; Multidisciplinary Digital Publishing Institute; Microorganisms; 12; 7; 6-2024; 1-17
2076-2607
CONICET Digital
CONICET
url http://hdl.handle.net/11336/260793
identifier_str_mv Ortiz Moyano, Francisco Ramiro; Dentice Maidana, Stefania; Imamura, Yoshiya; Elean, Mariano Daniel; Namai, Fu; et al.; Antagonistic Effects of Corynebacterium pseudodiphtheriticum 090104 on Respiratory Pathogens; Multidisciplinary Digital Publishing Institute; Microorganisms; 12; 7; 6-2024; 1-17
2076-2607
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/12/7/1295
info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms12071295
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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