The rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress

Autores
Raineri, Jesica; Wang, Songhu; Peleg, Zvi; Blumwald, Eduardo; Chan, Raquel Lia
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
OsWRKY47 is a divergent rice transcription factor belonging to the group II of the WRKY family. A transcriptomic analysis of the drought response of transgenic rice plants expressing PSARK::IPT, validated by qPCR, indicated that OsWRKY47 expression was induced under drought stress in PSARK::IPT plants. A PCR-assisted site selection assay (SELEX) of recombinant OsWRKY47 protein showed that the preferred sequence bound in vitro is (G/T)TTGACT. Bioinformatics analyses identified a number of gene targets of OsWRKY47; among these two genes encode a Calmodulin binding protein and a Cys-rich secretory protein. Using Oswrk47 knockout mutants and transgenic rice overexpressing OsWRKY47 we show that the transcription of these putative targets were regulated by OsWRKY47. Phenotypic analysis carried out with transgenic rice plants showed that Oswrky47 mutants displayed higher sensitivity to drought and reduced yield, while plants overexpressing OsWRKY47 were more tolerant.
Fil: Raineri, Jesica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Wang, Songhu. Chinese Academy of Sciences; República de China. University of California at Davis; Estados Unidos
Fil: Peleg, Zvi. The Hebrew University of Jerusalem; Israel. University of California at Davis; Estados Unidos
Fil: Blumwald, Eduardo. University of California at Davis; Estados Unidos
Fil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Materia
Delayed Senescence
Drought Tolerance
Oswrky47
P<Inf>Sark</Inf>::Ipt
Wrky Transcription Factor
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/39627
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stressRaineri, JesicaWang, SonghuPeleg, ZviBlumwald, EduardoChan, Raquel LiaDelayed SenescenceDrought ToleranceOswrky47P<Inf>Sark</Inf>::IptWrky Transcription Factorhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4OsWRKY47 is a divergent rice transcription factor belonging to the group II of the WRKY family. A transcriptomic analysis of the drought response of transgenic rice plants expressing PSARK::IPT, validated by qPCR, indicated that OsWRKY47 expression was induced under drought stress in PSARK::IPT plants. A PCR-assisted site selection assay (SELEX) of recombinant OsWRKY47 protein showed that the preferred sequence bound in vitro is (G/T)TTGACT. Bioinformatics analyses identified a number of gene targets of OsWRKY47; among these two genes encode a Calmodulin binding protein and a Cys-rich secretory protein. Using Oswrk47 knockout mutants and transgenic rice overexpressing OsWRKY47 we show that the transcription of these putative targets were regulated by OsWRKY47. Phenotypic analysis carried out with transgenic rice plants showed that Oswrky47 mutants displayed higher sensitivity to drought and reduced yield, while plants overexpressing OsWRKY47 were more tolerant.Fil: Raineri, Jesica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Wang, Songhu. Chinese Academy of Sciences; República de China. University of California at Davis; Estados UnidosFil: Peleg, Zvi. The Hebrew University of Jerusalem; Israel. University of California at Davis; Estados UnidosFil: Blumwald, Eduardo. University of California at Davis; Estados UnidosFil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaSpringer2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39627Raineri, Jesica; Wang, Songhu; Peleg, Zvi; Blumwald, Eduardo; Chan, Raquel Lia; The rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress; Springer; Plant Molecular Biology; 88; 4-5; 7-2015; 401-4130167-4412CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11103-015-0329-7info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11103-015-0329-7info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:26:44Zoai:ri.conicet.gov.ar:11336/39627instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:26:44.498CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress
title The rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress
spellingShingle The rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress
Raineri, Jesica
Delayed Senescence
Drought Tolerance
Oswrky47
P<Inf>Sark</Inf>::Ipt
Wrky Transcription Factor
title_short The rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress
title_full The rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress
title_fullStr The rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress
title_full_unstemmed The rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress
title_sort The rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress
dc.creator.none.fl_str_mv Raineri, Jesica
Wang, Songhu
Peleg, Zvi
Blumwald, Eduardo
Chan, Raquel Lia
author Raineri, Jesica
author_facet Raineri, Jesica
Wang, Songhu
Peleg, Zvi
Blumwald, Eduardo
Chan, Raquel Lia
author_role author
author2 Wang, Songhu
Peleg, Zvi
Blumwald, Eduardo
Chan, Raquel Lia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Delayed Senescence
Drought Tolerance
Oswrky47
P<Inf>Sark</Inf>::Ipt
Wrky Transcription Factor
topic Delayed Senescence
Drought Tolerance
Oswrky47
P<Inf>Sark</Inf>::Ipt
Wrky Transcription Factor
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv OsWRKY47 is a divergent rice transcription factor belonging to the group II of the WRKY family. A transcriptomic analysis of the drought response of transgenic rice plants expressing PSARK::IPT, validated by qPCR, indicated that OsWRKY47 expression was induced under drought stress in PSARK::IPT plants. A PCR-assisted site selection assay (SELEX) of recombinant OsWRKY47 protein showed that the preferred sequence bound in vitro is (G/T)TTGACT. Bioinformatics analyses identified a number of gene targets of OsWRKY47; among these two genes encode a Calmodulin binding protein and a Cys-rich secretory protein. Using Oswrk47 knockout mutants and transgenic rice overexpressing OsWRKY47 we show that the transcription of these putative targets were regulated by OsWRKY47. Phenotypic analysis carried out with transgenic rice plants showed that Oswrky47 mutants displayed higher sensitivity to drought and reduced yield, while plants overexpressing OsWRKY47 were more tolerant.
Fil: Raineri, Jesica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Wang, Songhu. Chinese Academy of Sciences; República de China. University of California at Davis; Estados Unidos
Fil: Peleg, Zvi. The Hebrew University of Jerusalem; Israel. University of California at Davis; Estados Unidos
Fil: Blumwald, Eduardo. University of California at Davis; Estados Unidos
Fil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
description OsWRKY47 is a divergent rice transcription factor belonging to the group II of the WRKY family. A transcriptomic analysis of the drought response of transgenic rice plants expressing PSARK::IPT, validated by qPCR, indicated that OsWRKY47 expression was induced under drought stress in PSARK::IPT plants. A PCR-assisted site selection assay (SELEX) of recombinant OsWRKY47 protein showed that the preferred sequence bound in vitro is (G/T)TTGACT. Bioinformatics analyses identified a number of gene targets of OsWRKY47; among these two genes encode a Calmodulin binding protein and a Cys-rich secretory protein. Using Oswrk47 knockout mutants and transgenic rice overexpressing OsWRKY47 we show that the transcription of these putative targets were regulated by OsWRKY47. Phenotypic analysis carried out with transgenic rice plants showed that Oswrky47 mutants displayed higher sensitivity to drought and reduced yield, while plants overexpressing OsWRKY47 were more tolerant.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39627
Raineri, Jesica; Wang, Songhu; Peleg, Zvi; Blumwald, Eduardo; Chan, Raquel Lia; The rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress; Springer; Plant Molecular Biology; 88; 4-5; 7-2015; 401-413
0167-4412
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39627
identifier_str_mv Raineri, Jesica; Wang, Songhu; Peleg, Zvi; Blumwald, Eduardo; Chan, Raquel Lia; The rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress; Springer; Plant Molecular Biology; 88; 4-5; 7-2015; 401-413
0167-4412
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s11103-015-0329-7
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11103-015-0329-7
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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