Multi-scale approach for the evaluation of bone mineralization in strontium ranelate-treated diabetic rats

Autores
Álvarez Lloret, Pedro; Fernández, Juan Manuel; Molinuevo, María Silvina; Lino, Agustina Berenice; Ferretti, José Luis; Capozza, Ricardo Francisco; Cortizo, Ana María; McCarthy, Antonio Desmond
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Long-term Diabetes mellitus can induce osteopenia, osteoporosis, an increase in the incidence of low-stress fractures and/or delayed fracture healing. Strontium ranelate (SrR) is a dual-action anti-osteoporotic agent whose use in individuals with diabetic osteopathy has not been adequately evaluated. In this study, we studied the effects of an oral treatment with SrR and/or experimental Diabetes on bone composition and biomechanics. Young male Wistar rats (half non-diabetic, half with streptozotocin/nicotinamide-induced Diabetes) were either untreated, or orally administered 625mg/kg/day of SrR for 6 weeks. After sacrifice, femora from all animals were evaluated by a multi-scale approach (X-ray diffraction, Fourier transform infrared spectroscopy, inductively-coupled plasma optical-emission spectrometry, static histomorphometry, pQCT and mechanical testing) to determine chemical, crystalline, and biomechanical properties. Untreated diabetic animals (versus untreated non-diabetic) showed a decrease in femoral mineral carbonate content, in cortical thickness and BMC, in trabecular osteocyte density, in maximum load supported at rupture and at yield point, and in overall toughness at mid-shaft. Treatment of diabetic animals with SrR further affected several parameters of bone (some already impaired by Diabetes): Crystallinity Index (indicating less mature apatite crystals); trabecular area, BMC and vBMD; maximum load at yield point and structural elastic rigidity. However, SrR was also able to prevent the Diabetes-induced decreases in trabecular osteocyte density (completely) and in bone ultimate strength at rupture (partially). Our results indicate that SrR treatment can partially but significantly prevent some bone structural mechanical properties as previously affected by Diabetes, but not others (which may even be worsened).
Fil: Álvarez Lloret, Pedro. Universidad de Oviedo; España
Fil: Fernández, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Molinuevo, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina
Fil: Lino, Agustina Berenice. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina
Fil: Ferretti, José Luis. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina
Fil: Capozza, Ricardo Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina
Fil: Cortizo, Ana María. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina
Fil: McCarthy, Antonio Desmond. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina
Materia
DIABETES MELLITUS
RANELATO DE ESTRONCIO
MINERALIZACIÓN ÓSEA
MICROESTRUCTURA ÓSEA
BIOMECÁNICA ÓSEA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102189
Acceder
id CONICETDig_f256d1b0279e8fc60ca1d35c34ea50d7
oai_identifier_str oai:ri.conicet.gov.ar:11336/102189
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Multi-scale approach for the evaluation of bone mineralization in strontium ranelate-treated diabetic ratsÁlvarez Lloret, PedroFernández, Juan ManuelMolinuevo, María SilvinaLino, Agustina BereniceFerretti, José LuisCapozza, Ricardo FranciscoCortizo, Ana MaríaMcCarthy, Antonio DesmondDIABETES MELLITUSRANELATO DE ESTRONCIOMINERALIZACIÓN ÓSEAMICROESTRUCTURA ÓSEABIOMECÁNICA ÓSEAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Long-term Diabetes mellitus can induce osteopenia, osteoporosis, an increase in the incidence of low-stress fractures and/or delayed fracture healing. Strontium ranelate (SrR) is a dual-action anti-osteoporotic agent whose use in individuals with diabetic osteopathy has not been adequately evaluated. In this study, we studied the effects of an oral treatment with SrR and/or experimental Diabetes on bone composition and biomechanics. Young male Wistar rats (half non-diabetic, half with streptozotocin/nicotinamide-induced Diabetes) were either untreated, or orally administered 625mg/kg/day of SrR for 6 weeks. After sacrifice, femora from all animals were evaluated by a multi-scale approach (X-ray diffraction, Fourier transform infrared spectroscopy, inductively-coupled plasma optical-emission spectrometry, static histomorphometry, pQCT and mechanical testing) to determine chemical, crystalline, and biomechanical properties. Untreated diabetic animals (versus untreated non-diabetic) showed a decrease in femoral mineral carbonate content, in cortical thickness and BMC, in trabecular osteocyte density, in maximum load supported at rupture and at yield point, and in overall toughness at mid-shaft. Treatment of diabetic animals with SrR further affected several parameters of bone (some already impaired by Diabetes): Crystallinity Index (indicating less mature apatite crystals); trabecular area, BMC and vBMD; maximum load at yield point and structural elastic rigidity. However, SrR was also able to prevent the Diabetes-induced decreases in trabecular osteocyte density (completely) and in bone ultimate strength at rupture (partially). Our results indicate that SrR treatment can partially but significantly prevent some bone structural mechanical properties as previously affected by Diabetes, but not others (which may even be worsened).Fil: Álvarez Lloret, Pedro. Universidad de Oviedo; EspañaFil: Fernández, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Molinuevo, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; ArgentinaFil: Lino, Agustina Berenice. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; ArgentinaFil: Ferretti, José Luis. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; ArgentinaFil: Capozza, Ricardo Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; ArgentinaFil: Cortizo, Ana María. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; ArgentinaFil: McCarthy, Antonio Desmond. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; ArgentinaHumana Press2018-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102189Álvarez Lloret, Pedro; Fernández, Juan Manuel; Molinuevo, María Silvina; Lino, Agustina Berenice; Ferretti, José Luis; et al.; Multi-scale approach for the evaluation of bone mineralization in strontium ranelate-treated diabetic rats; Humana Press; Biological Trace Element Research; 4-2018; 1-100163-4984CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s12011-018-1322-1info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s12011-018-1322-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:06Zoai:ri.conicet.gov.ar:11336/102189instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:06.65CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Multi-scale approach for the evaluation of bone mineralization in strontium ranelate-treated diabetic rats
title Multi-scale approach for the evaluation of bone mineralization in strontium ranelate-treated diabetic rats
spellingShingle Multi-scale approach for the evaluation of bone mineralization in strontium ranelate-treated diabetic rats
Álvarez Lloret, Pedro
DIABETES MELLITUS
RANELATO DE ESTRONCIO
MINERALIZACIÓN ÓSEA
MICROESTRUCTURA ÓSEA
BIOMECÁNICA ÓSEA
title_short Multi-scale approach for the evaluation of bone mineralization in strontium ranelate-treated diabetic rats
title_full Multi-scale approach for the evaluation of bone mineralization in strontium ranelate-treated diabetic rats
title_fullStr Multi-scale approach for the evaluation of bone mineralization in strontium ranelate-treated diabetic rats
title_full_unstemmed Multi-scale approach for the evaluation of bone mineralization in strontium ranelate-treated diabetic rats
title_sort Multi-scale approach for the evaluation of bone mineralization in strontium ranelate-treated diabetic rats
dc.creator.none.fl_str_mv Álvarez Lloret, Pedro
Fernández, Juan Manuel
Molinuevo, María Silvina
Lino, Agustina Berenice
Ferretti, José Luis
Capozza, Ricardo Francisco
Cortizo, Ana María
McCarthy, Antonio Desmond
author Álvarez Lloret, Pedro
author_facet Álvarez Lloret, Pedro
Fernández, Juan Manuel
Molinuevo, María Silvina
Lino, Agustina Berenice
Ferretti, José Luis
Capozza, Ricardo Francisco
Cortizo, Ana María
McCarthy, Antonio Desmond
author_role author
author2 Fernández, Juan Manuel
Molinuevo, María Silvina
Lino, Agustina Berenice
Ferretti, José Luis
Capozza, Ricardo Francisco
Cortizo, Ana María
McCarthy, Antonio Desmond
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DIABETES MELLITUS
RANELATO DE ESTRONCIO
MINERALIZACIÓN ÓSEA
MICROESTRUCTURA ÓSEA
BIOMECÁNICA ÓSEA
topic DIABETES MELLITUS
RANELATO DE ESTRONCIO
MINERALIZACIÓN ÓSEA
MICROESTRUCTURA ÓSEA
BIOMECÁNICA ÓSEA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Long-term Diabetes mellitus can induce osteopenia, osteoporosis, an increase in the incidence of low-stress fractures and/or delayed fracture healing. Strontium ranelate (SrR) is a dual-action anti-osteoporotic agent whose use in individuals with diabetic osteopathy has not been adequately evaluated. In this study, we studied the effects of an oral treatment with SrR and/or experimental Diabetes on bone composition and biomechanics. Young male Wistar rats (half non-diabetic, half with streptozotocin/nicotinamide-induced Diabetes) were either untreated, or orally administered 625mg/kg/day of SrR for 6 weeks. After sacrifice, femora from all animals were evaluated by a multi-scale approach (X-ray diffraction, Fourier transform infrared spectroscopy, inductively-coupled plasma optical-emission spectrometry, static histomorphometry, pQCT and mechanical testing) to determine chemical, crystalline, and biomechanical properties. Untreated diabetic animals (versus untreated non-diabetic) showed a decrease in femoral mineral carbonate content, in cortical thickness and BMC, in trabecular osteocyte density, in maximum load supported at rupture and at yield point, and in overall toughness at mid-shaft. Treatment of diabetic animals with SrR further affected several parameters of bone (some already impaired by Diabetes): Crystallinity Index (indicating less mature apatite crystals); trabecular area, BMC and vBMD; maximum load at yield point and structural elastic rigidity. However, SrR was also able to prevent the Diabetes-induced decreases in trabecular osteocyte density (completely) and in bone ultimate strength at rupture (partially). Our results indicate that SrR treatment can partially but significantly prevent some bone structural mechanical properties as previously affected by Diabetes, but not others (which may even be worsened).
Fil: Álvarez Lloret, Pedro. Universidad de Oviedo; España
Fil: Fernández, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Molinuevo, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina
Fil: Lino, Agustina Berenice. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina
Fil: Ferretti, José Luis. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina
Fil: Capozza, Ricardo Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina
Fil: Cortizo, Ana María. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina
Fil: McCarthy, Antonio Desmond. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina
description Long-term Diabetes mellitus can induce osteopenia, osteoporosis, an increase in the incidence of low-stress fractures and/or delayed fracture healing. Strontium ranelate (SrR) is a dual-action anti-osteoporotic agent whose use in individuals with diabetic osteopathy has not been adequately evaluated. In this study, we studied the effects of an oral treatment with SrR and/or experimental Diabetes on bone composition and biomechanics. Young male Wistar rats (half non-diabetic, half with streptozotocin/nicotinamide-induced Diabetes) were either untreated, or orally administered 625mg/kg/day of SrR for 6 weeks. After sacrifice, femora from all animals were evaluated by a multi-scale approach (X-ray diffraction, Fourier transform infrared spectroscopy, inductively-coupled plasma optical-emission spectrometry, static histomorphometry, pQCT and mechanical testing) to determine chemical, crystalline, and biomechanical properties. Untreated diabetic animals (versus untreated non-diabetic) showed a decrease in femoral mineral carbonate content, in cortical thickness and BMC, in trabecular osteocyte density, in maximum load supported at rupture and at yield point, and in overall toughness at mid-shaft. Treatment of diabetic animals with SrR further affected several parameters of bone (some already impaired by Diabetes): Crystallinity Index (indicating less mature apatite crystals); trabecular area, BMC and vBMD; maximum load at yield point and structural elastic rigidity. However, SrR was also able to prevent the Diabetes-induced decreases in trabecular osteocyte density (completely) and in bone ultimate strength at rupture (partially). Our results indicate that SrR treatment can partially but significantly prevent some bone structural mechanical properties as previously affected by Diabetes, but not others (which may even be worsened).
publishDate 2018
dc.date.none.fl_str_mv 2018-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102189
Álvarez Lloret, Pedro; Fernández, Juan Manuel; Molinuevo, María Silvina; Lino, Agustina Berenice; Ferretti, José Luis; et al.; Multi-scale approach for the evaluation of bone mineralization in strontium ranelate-treated diabetic rats; Humana Press; Biological Trace Element Research; 4-2018; 1-10
0163-4984
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102189
identifier_str_mv Álvarez Lloret, Pedro; Fernández, Juan Manuel; Molinuevo, María Silvina; Lino, Agustina Berenice; Ferretti, José Luis; et al.; Multi-scale approach for the evaluation of bone mineralization in strontium ranelate-treated diabetic rats; Humana Press; Biological Trace Element Research; 4-2018; 1-10
0163-4984
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s12011-018-1322-1
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s12011-018-1322-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Humana Press
publisher.none.fl_str_mv Humana Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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