Protective role against hydrogen peroxide and fibroblast stimulation via Ce-doped TiO2 nanostructured materials

Autores
Gravina, Noel; Maghni, Karim; Welman, Mélanie; Yahia, L'Hocine; Mbeh, Doris Antoinette; Messina, Paula Verónica
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Cerium oxide (CeO2) and Ce-doped nanostructured materials (NMs) are being seen as innovative therapeutic tools due to their exceptional antioxidant effects; nevertheless their bio-applications are still in their infancy. Methods: TiO2, Ce–TiO2 and CeO2–TiO2 NMs were synthesized by a bottom-up microemulsion-mediated strategy and calcined during 7 h at 650 °C under air flux. The samples were compared to elucidate the physicochemical characteristics that determine cellular uptake, toxicity and the influence of redox balance between the Ce3 +/Ce4 + on the cytoprotective role against an exogenous ROS source: H2O2. Fibroblasts were selected as a cell model because of their participation in wound healing and fibrotic diseases. Results: Ce–TiO2 NM obtained via sol–gel reaction chemistry of metallic organic precursors exerts a real cytoprotective effect against H2O2 over fibroblast proliferation, while CeO2 pre-formed nanoparticles incorporated to TiO2 crystalline matrix lead to a harmful CeO2–TiO2 material. TiO2 was processed by the same pathways as Ce–TiO2 and CeO2–TiO2 NM but did not elicit any adverse or protective influence compared to controls. Conclusions: It was found that the Ce atoms source and its concentration have a clear effect on material's physicochemical properties and its subsequent influence in the cellular response. It can induce a range of biological reactions that vary from cytotoxic to cytoprotective. General significance: Even though there are still some unresolved issues and challenges, the unique physical and chemical properties of Ce-based NMs are fascinating and versatile resources for different biomedical applications.
Fil: Gravina, Noel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Maghni, Karim. Hôpital du Sacré-Coeur-de-Montréal; Canadá
Fil: Welman, Mélanie. Hôpital du Sacré-Coeur-de-Montréal; Canadá
Fil: Yahia, L'Hocine. École Polytechnique de Montréal; Canadá
Fil: Mbeh, Doris Antoinette. École Polytechnique de Montréal; Canadá
Fil: Messina, Paula Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Materia
Anatase
Ce-Doped Tio2
Fibroblast
Nano-Ceria
Nanoparticles
Reactive Oxygen Species
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53813
Acceder
id CONICETDig_f168d4d05962199e6762af5bd5500f07
oai_identifier_str oai:ri.conicet.gov.ar:11336/53813
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Protective role against hydrogen peroxide and fibroblast stimulation via Ce-doped TiO2 nanostructured materialsGravina, NoelMaghni, KarimWelman, MélanieYahia, L'HocineMbeh, Doris AntoinetteMessina, Paula VerónicaAnataseCe-Doped Tio2FibroblastNano-CeriaNanoparticlesReactive Oxygen Specieshttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Background: Cerium oxide (CeO2) and Ce-doped nanostructured materials (NMs) are being seen as innovative therapeutic tools due to their exceptional antioxidant effects; nevertheless their bio-applications are still in their infancy. Methods: TiO2, Ce–TiO2 and CeO2–TiO2 NMs were synthesized by a bottom-up microemulsion-mediated strategy and calcined during 7 h at 650 °C under air flux. The samples were compared to elucidate the physicochemical characteristics that determine cellular uptake, toxicity and the influence of redox balance between the Ce3 +/Ce4 + on the cytoprotective role against an exogenous ROS source: H2O2. Fibroblasts were selected as a cell model because of their participation in wound healing and fibrotic diseases. Results: Ce–TiO2 NM obtained via sol–gel reaction chemistry of metallic organic precursors exerts a real cytoprotective effect against H2O2 over fibroblast proliferation, while CeO2 pre-formed nanoparticles incorporated to TiO2 crystalline matrix lead to a harmful CeO2–TiO2 material. TiO2 was processed by the same pathways as Ce–TiO2 and CeO2–TiO2 NM but did not elicit any adverse or protective influence compared to controls. Conclusions: It was found that the Ce atoms source and its concentration have a clear effect on material's physicochemical properties and its subsequent influence in the cellular response. It can induce a range of biological reactions that vary from cytotoxic to cytoprotective. General significance: Even though there are still some unresolved issues and challenges, the unique physical and chemical properties of Ce-based NMs are fascinating and versatile resources for different biomedical applications.Fil: Gravina, Noel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Maghni, Karim. Hôpital du Sacré-Coeur-de-Montréal; CanadáFil: Welman, Mélanie. Hôpital du Sacré-Coeur-de-Montréal; CanadáFil: Yahia, L'Hocine. École Polytechnique de Montréal; CanadáFil: Mbeh, Doris Antoinette. École Polytechnique de Montréal; CanadáFil: Messina, Paula Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaElsevier Science2016-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53813Gravina, Noel; Maghni, Karim; Welman, Mélanie; Yahia, L'Hocine; Mbeh, Doris Antoinette; et al.; Protective role against hydrogen peroxide and fibroblast stimulation via Ce-doped TiO2 nanostructured materials; Elsevier Science; Biochimica et Biophysica Acta- General Subjects; 1860; 2; 2-2016; 452-4640304-4165CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S030441651500327Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbagen.2015.12.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:05Zoai:ri.conicet.gov.ar:11336/53813instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:05.905CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Protective role against hydrogen peroxide and fibroblast stimulation via Ce-doped TiO2 nanostructured materials
title Protective role against hydrogen peroxide and fibroblast stimulation via Ce-doped TiO2 nanostructured materials
spellingShingle Protective role against hydrogen peroxide and fibroblast stimulation via Ce-doped TiO2 nanostructured materials
Gravina, Noel
Anatase
Ce-Doped Tio2
Fibroblast
Nano-Ceria
Nanoparticles
Reactive Oxygen Species
title_short Protective role against hydrogen peroxide and fibroblast stimulation via Ce-doped TiO2 nanostructured materials
title_full Protective role against hydrogen peroxide and fibroblast stimulation via Ce-doped TiO2 nanostructured materials
title_fullStr Protective role against hydrogen peroxide and fibroblast stimulation via Ce-doped TiO2 nanostructured materials
title_full_unstemmed Protective role against hydrogen peroxide and fibroblast stimulation via Ce-doped TiO2 nanostructured materials
title_sort Protective role against hydrogen peroxide and fibroblast stimulation via Ce-doped TiO2 nanostructured materials
dc.creator.none.fl_str_mv Gravina, Noel
Maghni, Karim
Welman, Mélanie
Yahia, L'Hocine
Mbeh, Doris Antoinette
Messina, Paula Verónica
author Gravina, Noel
author_facet Gravina, Noel
Maghni, Karim
Welman, Mélanie
Yahia, L'Hocine
Mbeh, Doris Antoinette
Messina, Paula Verónica
author_role author
author2 Maghni, Karim
Welman, Mélanie
Yahia, L'Hocine
Mbeh, Doris Antoinette
Messina, Paula Verónica
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Anatase
Ce-Doped Tio2
Fibroblast
Nano-Ceria
Nanoparticles
Reactive Oxygen Species
topic Anatase
Ce-Doped Tio2
Fibroblast
Nano-Ceria
Nanoparticles
Reactive Oxygen Species
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Background: Cerium oxide (CeO2) and Ce-doped nanostructured materials (NMs) are being seen as innovative therapeutic tools due to their exceptional antioxidant effects; nevertheless their bio-applications are still in their infancy. Methods: TiO2, Ce–TiO2 and CeO2–TiO2 NMs were synthesized by a bottom-up microemulsion-mediated strategy and calcined during 7 h at 650 °C under air flux. The samples were compared to elucidate the physicochemical characteristics that determine cellular uptake, toxicity and the influence of redox balance between the Ce3 +/Ce4 + on the cytoprotective role against an exogenous ROS source: H2O2. Fibroblasts were selected as a cell model because of their participation in wound healing and fibrotic diseases. Results: Ce–TiO2 NM obtained via sol–gel reaction chemistry of metallic organic precursors exerts a real cytoprotective effect against H2O2 over fibroblast proliferation, while CeO2 pre-formed nanoparticles incorporated to TiO2 crystalline matrix lead to a harmful CeO2–TiO2 material. TiO2 was processed by the same pathways as Ce–TiO2 and CeO2–TiO2 NM but did not elicit any adverse or protective influence compared to controls. Conclusions: It was found that the Ce atoms source and its concentration have a clear effect on material's physicochemical properties and its subsequent influence in the cellular response. It can induce a range of biological reactions that vary from cytotoxic to cytoprotective. General significance: Even though there are still some unresolved issues and challenges, the unique physical and chemical properties of Ce-based NMs are fascinating and versatile resources for different biomedical applications.
Fil: Gravina, Noel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Maghni, Karim. Hôpital du Sacré-Coeur-de-Montréal; Canadá
Fil: Welman, Mélanie. Hôpital du Sacré-Coeur-de-Montréal; Canadá
Fil: Yahia, L'Hocine. École Polytechnique de Montréal; Canadá
Fil: Mbeh, Doris Antoinette. École Polytechnique de Montréal; Canadá
Fil: Messina, Paula Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
description Background: Cerium oxide (CeO2) and Ce-doped nanostructured materials (NMs) are being seen as innovative therapeutic tools due to their exceptional antioxidant effects; nevertheless their bio-applications are still in their infancy. Methods: TiO2, Ce–TiO2 and CeO2–TiO2 NMs were synthesized by a bottom-up microemulsion-mediated strategy and calcined during 7 h at 650 °C under air flux. The samples were compared to elucidate the physicochemical characteristics that determine cellular uptake, toxicity and the influence of redox balance between the Ce3 +/Ce4 + on the cytoprotective role against an exogenous ROS source: H2O2. Fibroblasts were selected as a cell model because of their participation in wound healing and fibrotic diseases. Results: Ce–TiO2 NM obtained via sol–gel reaction chemistry of metallic organic precursors exerts a real cytoprotective effect against H2O2 over fibroblast proliferation, while CeO2 pre-formed nanoparticles incorporated to TiO2 crystalline matrix lead to a harmful CeO2–TiO2 material. TiO2 was processed by the same pathways as Ce–TiO2 and CeO2–TiO2 NM but did not elicit any adverse or protective influence compared to controls. Conclusions: It was found that the Ce atoms source and its concentration have a clear effect on material's physicochemical properties and its subsequent influence in the cellular response. It can induce a range of biological reactions that vary from cytotoxic to cytoprotective. General significance: Even though there are still some unresolved issues and challenges, the unique physical and chemical properties of Ce-based NMs are fascinating and versatile resources for different biomedical applications.
publishDate 2016
dc.date.none.fl_str_mv 2016-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53813
Gravina, Noel; Maghni, Karim; Welman, Mélanie; Yahia, L'Hocine; Mbeh, Doris Antoinette; et al.; Protective role against hydrogen peroxide and fibroblast stimulation via Ce-doped TiO2 nanostructured materials; Elsevier Science; Biochimica et Biophysica Acta- General Subjects; 1860; 2; 2-2016; 452-464
0304-4165
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53813
identifier_str_mv Gravina, Noel; Maghni, Karim; Welman, Mélanie; Yahia, L'Hocine; Mbeh, Doris Antoinette; et al.; Protective role against hydrogen peroxide and fibroblast stimulation via Ce-doped TiO2 nanostructured materials; Elsevier Science; Biochimica et Biophysica Acta- General Subjects; 1860; 2; 2-2016; 452-464
0304-4165
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S030441651500327X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbagen.2015.12.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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