Consumption of Lactobacillus casei fermented milk prevents Salmonella Reactive Arthritis by modulating IL-23/IL-17 expression
- Autores
- Noto Llana, Mariangeles; Sarnacki, Sebastian Hernan; Aya Castañeda, Maria del Rosario; Bernal, Maria Isabel; Cerquetti, Maria Cristina
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Reactive arthritis is the development of sterile joint inflammation as a sequel to a remote infection, often in the gut. We have previously shown that a low dose of S. enteritidis inoculated to streptomycin-pretreated mice generates a self-limiting enterocolitis suitable for studying reactive arthritis. Here we show that consumption of Lactobacillus casei prior to infection abolishes intestinal and joint inflammation triggered by Salmonella. BALB/c mice were sacrificed after infection; intestinal and joint samples were analyzed for histological changes and expression of cytokines. TNF- α was measured by ELISA and the expression of IL-1β, IL-6, IL-10, IL-17, IL-23 and TGF-β was assessed by qPCR. L. casei consumption prevented Salmonella-induced synovitis, the increment of TNF-α in knees and the increase of IL-17 expression in popliteal and inguinal lymph nodes. At intestinal level consumption of L. casei drastically diminished S. enteritidis invasiveness and shortened splenic persistence of the pathogen. Bacterial loads recovered at days 2 and 5 from Peyer?s patches were 10-fold lower in mice fed with L. casei. In accordance, we found that the augment in gut permeability induced during enterocolitis was decreased in those animals. Consumption of L. casei prior to infection failed to increase anti- inflammatory molecules such as IL-10 and TGF-β in the intestine. On the other hand, consumption of L. casei abrogated the expression of TNF-α, IL-17, IL-23, IL-1β and IL-6 in cecum and mesenteric lymph nodes. These cytokines are needed for differentiation of immune cells involved in the development of reactive arthritis such as Th17 and γδ T cells. Trafficking of these inflammatory cells from the gut to the joints has been proposed as a mechanism of generation of reactive arthritis. Our results suggest that L. casei consumption prevents Salmonella-induced synovitis by altering the intestinal milieu necessary for differentiation of cells involved in the generation of joint inflammation.
Fil: Noto Llana, Mariangeles. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica;
Fil: Sarnacki, Sebastian Hernan. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica;
Fil: Aya Castañeda, Maria del Rosario. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica;
Fil: Bernal, Maria Isabel. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica;
Fil: Cerquetti, Maria Cristina. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica; - Materia
-
SALMONELLA
ENTEROCOLITIS
PROBIOTICS
MICE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/527
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Consumption of Lactobacillus casei fermented milk prevents Salmonella Reactive Arthritis by modulating IL-23/IL-17 expressionNoto Llana, MariangelesSarnacki, Sebastian HernanAya Castañeda, Maria del RosarioBernal, Maria IsabelCerquetti, Maria CristinaSALMONELLAENTEROCOLITISPROBIOTICSMICEhttps://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6Reactive arthritis is the development of sterile joint inflammation as a sequel to a remote infection, often in the gut. We have previously shown that a low dose of S. enteritidis inoculated to streptomycin-pretreated mice generates a self-limiting enterocolitis suitable for studying reactive arthritis. Here we show that consumption of Lactobacillus casei prior to infection abolishes intestinal and joint inflammation triggered by Salmonella. BALB/c mice were sacrificed after infection; intestinal and joint samples were analyzed for histological changes and expression of cytokines. TNF- α was measured by ELISA and the expression of IL-1β, IL-6, IL-10, IL-17, IL-23 and TGF-β was assessed by qPCR. L. casei consumption prevented Salmonella-induced synovitis, the increment of TNF-α in knees and the increase of IL-17 expression in popliteal and inguinal lymph nodes. At intestinal level consumption of L. casei drastically diminished S. enteritidis invasiveness and shortened splenic persistence of the pathogen. Bacterial loads recovered at days 2 and 5 from Peyer?s patches were 10-fold lower in mice fed with L. casei. In accordance, we found that the augment in gut permeability induced during enterocolitis was decreased in those animals. Consumption of L. casei prior to infection failed to increase anti- inflammatory molecules such as IL-10 and TGF-β in the intestine. On the other hand, consumption of L. casei abrogated the expression of TNF-α, IL-17, IL-23, IL-1β and IL-6 in cecum and mesenteric lymph nodes. These cytokines are needed for differentiation of immune cells involved in the development of reactive arthritis such as Th17 and γδ T cells. Trafficking of these inflammatory cells from the gut to the joints has been proposed as a mechanism of generation of reactive arthritis. Our results suggest that L. casei consumption prevents Salmonella-induced synovitis by altering the intestinal milieu necessary for differentiation of cells involved in the generation of joint inflammation.Fil: Noto Llana, Mariangeles. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica;Fil: Sarnacki, Sebastian Hernan. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica;Fil: Aya Castañeda, Maria del Rosario. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica;Fil: Bernal, Maria Isabel. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica;Fil: Cerquetti, Maria Cristina. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica;Public Library Science2013-12-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/527Noto Llana, Mariangeles; Sarnacki, Sebastian Hernan; Aya Castañeda, Maria del Rosario; Bernal, Maria Isabel; Cerquetti, Maria Cristina; Consumption of Lactobacillus casei fermented milk prevents Salmonella Reactive Arthritis by modulating IL-23/IL-17 expression; Public Library Science; Plos One; 10-12-2013;1932-6203enginfo:eu-repo/semantics/altIdentifier/url/http://www.plosone.orginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:16:31Zoai:ri.conicet.gov.ar:11336/527instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:16:32.038CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Consumption of Lactobacillus casei fermented milk prevents Salmonella Reactive Arthritis by modulating IL-23/IL-17 expression |
title |
Consumption of Lactobacillus casei fermented milk prevents Salmonella Reactive Arthritis by modulating IL-23/IL-17 expression |
spellingShingle |
Consumption of Lactobacillus casei fermented milk prevents Salmonella Reactive Arthritis by modulating IL-23/IL-17 expression Noto Llana, Mariangeles SALMONELLA ENTEROCOLITIS PROBIOTICS MICE |
title_short |
Consumption of Lactobacillus casei fermented milk prevents Salmonella Reactive Arthritis by modulating IL-23/IL-17 expression |
title_full |
Consumption of Lactobacillus casei fermented milk prevents Salmonella Reactive Arthritis by modulating IL-23/IL-17 expression |
title_fullStr |
Consumption of Lactobacillus casei fermented milk prevents Salmonella Reactive Arthritis by modulating IL-23/IL-17 expression |
title_full_unstemmed |
Consumption of Lactobacillus casei fermented milk prevents Salmonella Reactive Arthritis by modulating IL-23/IL-17 expression |
title_sort |
Consumption of Lactobacillus casei fermented milk prevents Salmonella Reactive Arthritis by modulating IL-23/IL-17 expression |
dc.creator.none.fl_str_mv |
Noto Llana, Mariangeles Sarnacki, Sebastian Hernan Aya Castañeda, Maria del Rosario Bernal, Maria Isabel Cerquetti, Maria Cristina |
author |
Noto Llana, Mariangeles |
author_facet |
Noto Llana, Mariangeles Sarnacki, Sebastian Hernan Aya Castañeda, Maria del Rosario Bernal, Maria Isabel Cerquetti, Maria Cristina |
author_role |
author |
author2 |
Sarnacki, Sebastian Hernan Aya Castañeda, Maria del Rosario Bernal, Maria Isabel Cerquetti, Maria Cristina |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
SALMONELLA ENTEROCOLITIS PROBIOTICS MICE |
topic |
SALMONELLA ENTEROCOLITIS PROBIOTICS MICE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/1.6 |
dc.description.none.fl_txt_mv |
Reactive arthritis is the development of sterile joint inflammation as a sequel to a remote infection, often in the gut. We have previously shown that a low dose of S. enteritidis inoculated to streptomycin-pretreated mice generates a self-limiting enterocolitis suitable for studying reactive arthritis. Here we show that consumption of Lactobacillus casei prior to infection abolishes intestinal and joint inflammation triggered by Salmonella. BALB/c mice were sacrificed after infection; intestinal and joint samples were analyzed for histological changes and expression of cytokines. TNF- α was measured by ELISA and the expression of IL-1β, IL-6, IL-10, IL-17, IL-23 and TGF-β was assessed by qPCR. L. casei consumption prevented Salmonella-induced synovitis, the increment of TNF-α in knees and the increase of IL-17 expression in popliteal and inguinal lymph nodes. At intestinal level consumption of L. casei drastically diminished S. enteritidis invasiveness and shortened splenic persistence of the pathogen. Bacterial loads recovered at days 2 and 5 from Peyer?s patches were 10-fold lower in mice fed with L. casei. In accordance, we found that the augment in gut permeability induced during enterocolitis was decreased in those animals. Consumption of L. casei prior to infection failed to increase anti- inflammatory molecules such as IL-10 and TGF-β in the intestine. On the other hand, consumption of L. casei abrogated the expression of TNF-α, IL-17, IL-23, IL-1β and IL-6 in cecum and mesenteric lymph nodes. These cytokines are needed for differentiation of immune cells involved in the development of reactive arthritis such as Th17 and γδ T cells. Trafficking of these inflammatory cells from the gut to the joints has been proposed as a mechanism of generation of reactive arthritis. Our results suggest that L. casei consumption prevents Salmonella-induced synovitis by altering the intestinal milieu necessary for differentiation of cells involved in the generation of joint inflammation. Fil: Noto Llana, Mariangeles. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica; Fil: Sarnacki, Sebastian Hernan. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica; Fil: Aya Castañeda, Maria del Rosario. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica; Fil: Bernal, Maria Isabel. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica; Fil: Cerquetti, Maria Cristina. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica; |
description |
Reactive arthritis is the development of sterile joint inflammation as a sequel to a remote infection, often in the gut. We have previously shown that a low dose of S. enteritidis inoculated to streptomycin-pretreated mice generates a self-limiting enterocolitis suitable for studying reactive arthritis. Here we show that consumption of Lactobacillus casei prior to infection abolishes intestinal and joint inflammation triggered by Salmonella. BALB/c mice were sacrificed after infection; intestinal and joint samples were analyzed for histological changes and expression of cytokines. TNF- α was measured by ELISA and the expression of IL-1β, IL-6, IL-10, IL-17, IL-23 and TGF-β was assessed by qPCR. L. casei consumption prevented Salmonella-induced synovitis, the increment of TNF-α in knees and the increase of IL-17 expression in popliteal and inguinal lymph nodes. At intestinal level consumption of L. casei drastically diminished S. enteritidis invasiveness and shortened splenic persistence of the pathogen. Bacterial loads recovered at days 2 and 5 from Peyer?s patches were 10-fold lower in mice fed with L. casei. In accordance, we found that the augment in gut permeability induced during enterocolitis was decreased in those animals. Consumption of L. casei prior to infection failed to increase anti- inflammatory molecules such as IL-10 and TGF-β in the intestine. On the other hand, consumption of L. casei abrogated the expression of TNF-α, IL-17, IL-23, IL-1β and IL-6 in cecum and mesenteric lymph nodes. These cytokines are needed for differentiation of immune cells involved in the development of reactive arthritis such as Th17 and γδ T cells. Trafficking of these inflammatory cells from the gut to the joints has been proposed as a mechanism of generation of reactive arthritis. Our results suggest that L. casei consumption prevents Salmonella-induced synovitis by altering the intestinal milieu necessary for differentiation of cells involved in the generation of joint inflammation. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/527 Noto Llana, Mariangeles; Sarnacki, Sebastian Hernan; Aya Castañeda, Maria del Rosario; Bernal, Maria Isabel; Cerquetti, Maria Cristina; Consumption of Lactobacillus casei fermented milk prevents Salmonella Reactive Arthritis by modulating IL-23/IL-17 expression; Public Library Science; Plos One; 10-12-2013; 1932-6203 |
url |
http://hdl.handle.net/11336/527 |
identifier_str_mv |
Noto Llana, Mariangeles; Sarnacki, Sebastian Hernan; Aya Castañeda, Maria del Rosario; Bernal, Maria Isabel; Cerquetti, Maria Cristina; Consumption of Lactobacillus casei fermented milk prevents Salmonella Reactive Arthritis by modulating IL-23/IL-17 expression; Public Library Science; Plos One; 10-12-2013; 1932-6203 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.plosone.org |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614110996594688 |
score |
13.070432 |