Consumption of Lactobacillus casei fermented milk prevents Salmonella Reactive Arthritis by modulating IL-23/IL-17 expression

Autores
Noto Llana, Mariangeles; Sarnacki, Sebastian Hernan; Aya Castañeda, Maria del Rosario; Bernal, Maria Isabel; Cerquetti, Maria Cristina
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Reactive arthritis is the development of sterile joint inflammation as a sequel to a remote infection, often in the gut. We have previously shown that a low dose of S. enteritidis inoculated to streptomycin-pretreated mice generates a self-limiting enterocolitis suitable for studying reactive arthritis. Here we show that consumption of Lactobacillus casei prior to infection abolishes intestinal and joint inflammation triggered by Salmonella. BALB/c mice were sacrificed after infection; intestinal and joint samples were analyzed for histological changes and expression of cytokines. TNF- α was measured by ELISA and the expression of IL-1β, IL-6, IL-10, IL-17, IL-23 and TGF-β was assessed by qPCR. L. casei consumption prevented Salmonella-induced synovitis, the increment of TNF-α in knees and the increase of IL-17 expression in popliteal and inguinal lymph nodes. At intestinal level consumption of L. casei drastically diminished S. enteritidis invasiveness and shortened splenic persistence of the pathogen. Bacterial loads recovered at days 2 and 5 from Peyer?s patches were 10-fold lower in mice fed with L. casei. In accordance, we found that the augment in gut permeability induced during enterocolitis was decreased in those animals. Consumption of L. casei prior to infection failed to increase anti- inflammatory molecules such as IL-10 and TGF-β in the intestine. On the other hand, consumption of L. casei abrogated the expression of TNF-α, IL-17, IL-23, IL-1β and IL-6 in cecum and mesenteric lymph nodes. These cytokines are needed for differentiation of immune cells involved in the development of reactive arthritis such as Th17 and γδ T cells. Trafficking of these inflammatory cells from the gut to the joints has been proposed as a mechanism of generation of reactive arthritis. Our results suggest that L. casei consumption prevents Salmonella-induced synovitis by altering the intestinal milieu necessary for differentiation of cells involved in the generation of joint inflammation.
Fil: Noto Llana, Mariangeles. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica;
Fil: Sarnacki, Sebastian Hernan. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica;
Fil: Aya Castañeda, Maria del Rosario. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica;
Fil: Bernal, Maria Isabel. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica;
Fil: Cerquetti, Maria Cristina. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica;
Materia
SALMONELLA
ENTEROCOLITIS
PROBIOTICS
MICE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/527

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oai_identifier_str oai:ri.conicet.gov.ar:11336/527
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Consumption of Lactobacillus casei fermented milk prevents Salmonella Reactive Arthritis by modulating IL-23/IL-17 expressionNoto Llana, MariangelesSarnacki, Sebastian HernanAya Castañeda, Maria del RosarioBernal, Maria IsabelCerquetti, Maria CristinaSALMONELLAENTEROCOLITISPROBIOTICSMICEhttps://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6Reactive arthritis is the development of sterile joint inflammation as a sequel to a remote infection, often in the gut. We have previously shown that a low dose of S. enteritidis inoculated to streptomycin-pretreated mice generates a self-limiting enterocolitis suitable for studying reactive arthritis. Here we show that consumption of Lactobacillus casei prior to infection abolishes intestinal and joint inflammation triggered by Salmonella. BALB/c mice were sacrificed after infection; intestinal and joint samples were analyzed for histological changes and expression of cytokines. TNF- α was measured by ELISA and the expression of IL-1β, IL-6, IL-10, IL-17, IL-23 and TGF-β was assessed by qPCR. L. casei consumption prevented Salmonella-induced synovitis, the increment of TNF-α in knees and the increase of IL-17 expression in popliteal and inguinal lymph nodes. At intestinal level consumption of L. casei drastically diminished S. enteritidis invasiveness and shortened splenic persistence of the pathogen. Bacterial loads recovered at days 2 and 5 from Peyer?s patches were 10-fold lower in mice fed with L. casei. In accordance, we found that the augment in gut permeability induced during enterocolitis was decreased in those animals. Consumption of L. casei prior to infection failed to increase anti- inflammatory molecules such as IL-10 and TGF-β in the intestine. On the other hand, consumption of L. casei abrogated the expression of TNF-α, IL-17, IL-23, IL-1β and IL-6 in cecum and mesenteric lymph nodes. These cytokines are needed for differentiation of immune cells involved in the development of reactive arthritis such as Th17 and γδ T cells. Trafficking of these inflammatory cells from the gut to the joints has been proposed as a mechanism of generation of reactive arthritis. Our results suggest that L. casei consumption prevents Salmonella-induced synovitis by altering the intestinal milieu necessary for differentiation of cells involved in the generation of joint inflammation.Fil: Noto Llana, Mariangeles. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica;Fil: Sarnacki, Sebastian Hernan. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica;Fil: Aya Castañeda, Maria del Rosario. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica;Fil: Bernal, Maria Isabel. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica;Fil: Cerquetti, Maria Cristina. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica;Public Library Science2013-12-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/527Noto Llana, Mariangeles; Sarnacki, Sebastian Hernan; Aya Castañeda, Maria del Rosario; Bernal, Maria Isabel; Cerquetti, Maria Cristina; Consumption of Lactobacillus casei fermented milk prevents Salmonella Reactive Arthritis by modulating IL-23/IL-17 expression; Public Library Science; Plos One; 10-12-2013;1932-6203enginfo:eu-repo/semantics/altIdentifier/url/http://www.plosone.orginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:16:31Zoai:ri.conicet.gov.ar:11336/527instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:16:32.038CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Consumption of Lactobacillus casei fermented milk prevents Salmonella Reactive Arthritis by modulating IL-23/IL-17 expression
title Consumption of Lactobacillus casei fermented milk prevents Salmonella Reactive Arthritis by modulating IL-23/IL-17 expression
spellingShingle Consumption of Lactobacillus casei fermented milk prevents Salmonella Reactive Arthritis by modulating IL-23/IL-17 expression
Noto Llana, Mariangeles
SALMONELLA
ENTEROCOLITIS
PROBIOTICS
MICE
title_short Consumption of Lactobacillus casei fermented milk prevents Salmonella Reactive Arthritis by modulating IL-23/IL-17 expression
title_full Consumption of Lactobacillus casei fermented milk prevents Salmonella Reactive Arthritis by modulating IL-23/IL-17 expression
title_fullStr Consumption of Lactobacillus casei fermented milk prevents Salmonella Reactive Arthritis by modulating IL-23/IL-17 expression
title_full_unstemmed Consumption of Lactobacillus casei fermented milk prevents Salmonella Reactive Arthritis by modulating IL-23/IL-17 expression
title_sort Consumption of Lactobacillus casei fermented milk prevents Salmonella Reactive Arthritis by modulating IL-23/IL-17 expression
dc.creator.none.fl_str_mv Noto Llana, Mariangeles
Sarnacki, Sebastian Hernan
Aya Castañeda, Maria del Rosario
Bernal, Maria Isabel
Cerquetti, Maria Cristina
author Noto Llana, Mariangeles
author_facet Noto Llana, Mariangeles
Sarnacki, Sebastian Hernan
Aya Castañeda, Maria del Rosario
Bernal, Maria Isabel
Cerquetti, Maria Cristina
author_role author
author2 Sarnacki, Sebastian Hernan
Aya Castañeda, Maria del Rosario
Bernal, Maria Isabel
Cerquetti, Maria Cristina
author2_role author
author
author
author
dc.subject.none.fl_str_mv SALMONELLA
ENTEROCOLITIS
PROBIOTICS
MICE
topic SALMONELLA
ENTEROCOLITIS
PROBIOTICS
MICE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
dc.description.none.fl_txt_mv Reactive arthritis is the development of sterile joint inflammation as a sequel to a remote infection, often in the gut. We have previously shown that a low dose of S. enteritidis inoculated to streptomycin-pretreated mice generates a self-limiting enterocolitis suitable for studying reactive arthritis. Here we show that consumption of Lactobacillus casei prior to infection abolishes intestinal and joint inflammation triggered by Salmonella. BALB/c mice were sacrificed after infection; intestinal and joint samples were analyzed for histological changes and expression of cytokines. TNF- α was measured by ELISA and the expression of IL-1β, IL-6, IL-10, IL-17, IL-23 and TGF-β was assessed by qPCR. L. casei consumption prevented Salmonella-induced synovitis, the increment of TNF-α in knees and the increase of IL-17 expression in popliteal and inguinal lymph nodes. At intestinal level consumption of L. casei drastically diminished S. enteritidis invasiveness and shortened splenic persistence of the pathogen. Bacterial loads recovered at days 2 and 5 from Peyer?s patches were 10-fold lower in mice fed with L. casei. In accordance, we found that the augment in gut permeability induced during enterocolitis was decreased in those animals. Consumption of L. casei prior to infection failed to increase anti- inflammatory molecules such as IL-10 and TGF-β in the intestine. On the other hand, consumption of L. casei abrogated the expression of TNF-α, IL-17, IL-23, IL-1β and IL-6 in cecum and mesenteric lymph nodes. These cytokines are needed for differentiation of immune cells involved in the development of reactive arthritis such as Th17 and γδ T cells. Trafficking of these inflammatory cells from the gut to the joints has been proposed as a mechanism of generation of reactive arthritis. Our results suggest that L. casei consumption prevents Salmonella-induced synovitis by altering the intestinal milieu necessary for differentiation of cells involved in the generation of joint inflammation.
Fil: Noto Llana, Mariangeles. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica;
Fil: Sarnacki, Sebastian Hernan. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica;
Fil: Aya Castañeda, Maria del Rosario. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica;
Fil: Bernal, Maria Isabel. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica;
Fil: Cerquetti, Maria Cristina. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones En Microbiologia y Parasitologia Medica;
description Reactive arthritis is the development of sterile joint inflammation as a sequel to a remote infection, often in the gut. We have previously shown that a low dose of S. enteritidis inoculated to streptomycin-pretreated mice generates a self-limiting enterocolitis suitable for studying reactive arthritis. Here we show that consumption of Lactobacillus casei prior to infection abolishes intestinal and joint inflammation triggered by Salmonella. BALB/c mice were sacrificed after infection; intestinal and joint samples were analyzed for histological changes and expression of cytokines. TNF- α was measured by ELISA and the expression of IL-1β, IL-6, IL-10, IL-17, IL-23 and TGF-β was assessed by qPCR. L. casei consumption prevented Salmonella-induced synovitis, the increment of TNF-α in knees and the increase of IL-17 expression in popliteal and inguinal lymph nodes. At intestinal level consumption of L. casei drastically diminished S. enteritidis invasiveness and shortened splenic persistence of the pathogen. Bacterial loads recovered at days 2 and 5 from Peyer?s patches were 10-fold lower in mice fed with L. casei. In accordance, we found that the augment in gut permeability induced during enterocolitis was decreased in those animals. Consumption of L. casei prior to infection failed to increase anti- inflammatory molecules such as IL-10 and TGF-β in the intestine. On the other hand, consumption of L. casei abrogated the expression of TNF-α, IL-17, IL-23, IL-1β and IL-6 in cecum and mesenteric lymph nodes. These cytokines are needed for differentiation of immune cells involved in the development of reactive arthritis such as Th17 and γδ T cells. Trafficking of these inflammatory cells from the gut to the joints has been proposed as a mechanism of generation of reactive arthritis. Our results suggest that L. casei consumption prevents Salmonella-induced synovitis by altering the intestinal milieu necessary for differentiation of cells involved in the generation of joint inflammation.
publishDate 2013
dc.date.none.fl_str_mv 2013-12-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/527
Noto Llana, Mariangeles; Sarnacki, Sebastian Hernan; Aya Castañeda, Maria del Rosario; Bernal, Maria Isabel; Cerquetti, Maria Cristina; Consumption of Lactobacillus casei fermented milk prevents Salmonella Reactive Arthritis by modulating IL-23/IL-17 expression; Public Library Science; Plos One; 10-12-2013;
1932-6203
url http://hdl.handle.net/11336/527
identifier_str_mv Noto Llana, Mariangeles; Sarnacki, Sebastian Hernan; Aya Castañeda, Maria del Rosario; Bernal, Maria Isabel; Cerquetti, Maria Cristina; Consumption of Lactobacillus casei fermented milk prevents Salmonella Reactive Arthritis by modulating IL-23/IL-17 expression; Public Library Science; Plos One; 10-12-2013;
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.plosone.org
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Science
publisher.none.fl_str_mv Public Library Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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