Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide

Autores
dos Santos, Jose C. S.; Bonazza, Horacio; de Matos, Leonardo J. B. L.; Carneiro, Elizabete A.; Barbosa, Oveimar; Fernandez Lafuente, Roberto; Gonçalves, Luciana R.B.; de Sant' Ana, Hosiberto B.; Aguiar, Rílvia S. Santiago
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Enzymatic synthesis of fatty acid ethyl esters in supercritical and near-critical carbon dioxide using lipase B from Candida antarctica (CALB) immobilized onto chitosan is here investigated. The objective was to evaluate the operational parameters that influence the enzymatic esterification reaction between oleic acid and ethanol, conducted in supercritical carbon dioxide, as a route to produce ethyl oleate. Biocatalyst has been prepared through the immobilization of CALB by covalent bond using chitosan activated with glycidol, ethylenediamine (EDA) and glutaraldehyde as support. In order to determine the best operational conditions of the esterification reaction, an experimental design (23) was conducted to evaluate the effect of the following parameters: alcohol to oil molar ratios, reaction time and temperature. The maximum conversion was 46.9% at a temperature of 29.9 °C, ethanol to oleic acid molar ratio equal to 4.50:1, and a reaction time of 6.5 hours. Additionally, the removal of water from the medium, by using zeolite, promoted an increase in the conversion of oleic acid into ethyl esters. These results show that the combined use of supercritical carbon dioxide and zeolite favors the enzymatic esterification reaction, increasing the yields.
Fil: dos Santos, Jose C. S.. Universidade da Integração Internacional da Lusofonia Afro-Brasileira; Brasil
Fil: Bonazza, Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: de Matos, Leonardo J. B. L.. Universidade Federal do Ceará; Brasil
Fil: Carneiro, Elizabete A.. Universidade Federal do Ceará; Brasil
Fil: Barbosa, Oveimar. Universidad del Tolima; Colombia
Fil: Fernandez Lafuente, Roberto. Universidad Autónoma de Madrid; España
Fil: Gonçalves, Luciana R.B.. Universidade Federal do Ceará; Brasil
Fil: de Sant' Ana, Hosiberto B.. Universidade Federal do Ceará; Brasil
Fil: Aguiar, Rílvia S. Santiago. Universidade Federal do Ceará; Brasil
Materia
CalB
Lipase
chitosan
synthesis ethyl oleate
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/20798
Acceder
id CONICETDig_f102c9d723e6218d63378d1c1048ac02
oai_identifier_str oai:ri.conicet.gov.ar:11336/20798
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxidedos Santos, Jose C. S.Bonazza, Horaciode Matos, Leonardo J. B. L.Carneiro, Elizabete A.Barbosa, OveimarFernandez Lafuente, RobertoGonçalves, Luciana R.B.de Sant' Ana, Hosiberto B.Aguiar, Rílvia S. SantiagoCalBLipasechitosansynthesis ethyl oleatehttps://purl.org/becyt/ford/2.4https://purl.org/becyt/ford/2Enzymatic synthesis of fatty acid ethyl esters in supercritical and near-critical carbon dioxide using lipase B from Candida antarctica (CALB) immobilized onto chitosan is here investigated. The objective was to evaluate the operational parameters that influence the enzymatic esterification reaction between oleic acid and ethanol, conducted in supercritical carbon dioxide, as a route to produce ethyl oleate. Biocatalyst has been prepared through the immobilization of CALB by covalent bond using chitosan activated with glycidol, ethylenediamine (EDA) and glutaraldehyde as support. In order to determine the best operational conditions of the esterification reaction, an experimental design (23) was conducted to evaluate the effect of the following parameters: alcohol to oil molar ratios, reaction time and temperature. The maximum conversion was 46.9% at a temperature of 29.9 °C, ethanol to oleic acid molar ratio equal to 4.50:1, and a reaction time of 6.5 hours. Additionally, the removal of water from the medium, by using zeolite, promoted an increase in the conversion of oleic acid into ethyl esters. These results show that the combined use of supercritical carbon dioxide and zeolite favors the enzymatic esterification reaction, increasing the yields.Fil: dos Santos, Jose C. S.. Universidade da Integração Internacional da Lusofonia Afro-Brasileira; BrasilFil: Bonazza, Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: de Matos, Leonardo J. B. L.. Universidade Federal do Ceará; BrasilFil: Carneiro, Elizabete A.. Universidade Federal do Ceará; BrasilFil: Barbosa, Oveimar. Universidad del Tolima; ColombiaFil: Fernandez Lafuente, Roberto. Universidad Autónoma de Madrid; EspañaFil: Gonçalves, Luciana R.B.. Universidade Federal do Ceará; BrasilFil: de Sant' Ana, Hosiberto B.. Universidade Federal do Ceará; BrasilFil: Aguiar, Rílvia S. Santiago. Universidade Federal do Ceará; BrasilElsevier2017-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/20798dos Santos, Jose C. S.; Bonazza, Horacio; de Matos, Leonardo J. B. L.; Carneiro, Elizabete A.; Barbosa, Oveimar; et al.; Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide; Elsevier; Biotechnology Reports; 14; 3-2017; 16-262215-017XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S2215017X16300911info:eu-repo/semantics/altIdentifier/doi/10.1016/j.btre.2017.02.003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:42:17Zoai:ri.conicet.gov.ar:11336/20798instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:42:17.471CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide
title Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide
spellingShingle Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide
dos Santos, Jose C. S.
CalB
Lipase
chitosan
synthesis ethyl oleate
title_short Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide
title_full Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide
title_fullStr Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide
title_full_unstemmed Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide
title_sort Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide
dc.creator.none.fl_str_mv dos Santos, Jose C. S.
Bonazza, Horacio
de Matos, Leonardo J. B. L.
Carneiro, Elizabete A.
Barbosa, Oveimar
Fernandez Lafuente, Roberto
Gonçalves, Luciana R.B.
de Sant' Ana, Hosiberto B.
Aguiar, Rílvia S. Santiago
author dos Santos, Jose C. S.
author_facet dos Santos, Jose C. S.
Bonazza, Horacio
de Matos, Leonardo J. B. L.
Carneiro, Elizabete A.
Barbosa, Oveimar
Fernandez Lafuente, Roberto
Gonçalves, Luciana R.B.
de Sant' Ana, Hosiberto B.
Aguiar, Rílvia S. Santiago
author_role author
author2 Bonazza, Horacio
de Matos, Leonardo J. B. L.
Carneiro, Elizabete A.
Barbosa, Oveimar
Fernandez Lafuente, Roberto
Gonçalves, Luciana R.B.
de Sant' Ana, Hosiberto B.
Aguiar, Rílvia S. Santiago
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CalB
Lipase
chitosan
synthesis ethyl oleate
topic CalB
Lipase
chitosan
synthesis ethyl oleate
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.4
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Enzymatic synthesis of fatty acid ethyl esters in supercritical and near-critical carbon dioxide using lipase B from Candida antarctica (CALB) immobilized onto chitosan is here investigated. The objective was to evaluate the operational parameters that influence the enzymatic esterification reaction between oleic acid and ethanol, conducted in supercritical carbon dioxide, as a route to produce ethyl oleate. Biocatalyst has been prepared through the immobilization of CALB by covalent bond using chitosan activated with glycidol, ethylenediamine (EDA) and glutaraldehyde as support. In order to determine the best operational conditions of the esterification reaction, an experimental design (23) was conducted to evaluate the effect of the following parameters: alcohol to oil molar ratios, reaction time and temperature. The maximum conversion was 46.9% at a temperature of 29.9 °C, ethanol to oleic acid molar ratio equal to 4.50:1, and a reaction time of 6.5 hours. Additionally, the removal of water from the medium, by using zeolite, promoted an increase in the conversion of oleic acid into ethyl esters. These results show that the combined use of supercritical carbon dioxide and zeolite favors the enzymatic esterification reaction, increasing the yields.
Fil: dos Santos, Jose C. S.. Universidade da Integração Internacional da Lusofonia Afro-Brasileira; Brasil
Fil: Bonazza, Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: de Matos, Leonardo J. B. L.. Universidade Federal do Ceará; Brasil
Fil: Carneiro, Elizabete A.. Universidade Federal do Ceará; Brasil
Fil: Barbosa, Oveimar. Universidad del Tolima; Colombia
Fil: Fernandez Lafuente, Roberto. Universidad Autónoma de Madrid; España
Fil: Gonçalves, Luciana R.B.. Universidade Federal do Ceará; Brasil
Fil: de Sant' Ana, Hosiberto B.. Universidade Federal do Ceará; Brasil
Fil: Aguiar, Rílvia S. Santiago. Universidade Federal do Ceará; Brasil
description Enzymatic synthesis of fatty acid ethyl esters in supercritical and near-critical carbon dioxide using lipase B from Candida antarctica (CALB) immobilized onto chitosan is here investigated. The objective was to evaluate the operational parameters that influence the enzymatic esterification reaction between oleic acid and ethanol, conducted in supercritical carbon dioxide, as a route to produce ethyl oleate. Biocatalyst has been prepared through the immobilization of CALB by covalent bond using chitosan activated with glycidol, ethylenediamine (EDA) and glutaraldehyde as support. In order to determine the best operational conditions of the esterification reaction, an experimental design (23) was conducted to evaluate the effect of the following parameters: alcohol to oil molar ratios, reaction time and temperature. The maximum conversion was 46.9% at a temperature of 29.9 °C, ethanol to oleic acid molar ratio equal to 4.50:1, and a reaction time of 6.5 hours. Additionally, the removal of water from the medium, by using zeolite, promoted an increase in the conversion of oleic acid into ethyl esters. These results show that the combined use of supercritical carbon dioxide and zeolite favors the enzymatic esterification reaction, increasing the yields.
publishDate 2017
dc.date.none.fl_str_mv 2017-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/20798
dos Santos, Jose C. S.; Bonazza, Horacio; de Matos, Leonardo J. B. L.; Carneiro, Elizabete A.; Barbosa, Oveimar; et al.; Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide; Elsevier; Biotechnology Reports; 14; 3-2017; 16-26
2215-017X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/20798
identifier_str_mv dos Santos, Jose C. S.; Bonazza, Horacio; de Matos, Leonardo J. B. L.; Carneiro, Elizabete A.; Barbosa, Oveimar; et al.; Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide; Elsevier; Biotechnology Reports; 14; 3-2017; 16-26
2215-017X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S2215017X16300911
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.btre.2017.02.003
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613332205568000
score 13.070432

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