Preparation and modification of chitosan particles for Rhizomucor miehei lipase immobilization

Autores
Palla, Camila Andrea; Pacheco, Consuelo; Carrin, Maria Elena
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chitosan particles, suitable as immobilization support, were prepared by precipitation and modified by reductive amination in order to graft linear aliphatic chains of 12 carbon atoms to their native amine groups. Their physical characterization was performed by different techniques: differential scanning calorimetry (DSC), X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), air-water contact angle analysis, among others. Lipases from Rhizomucor miehei (RM) were immobilized by adsorption at low ionic strength onto different modified chitosan microspheres. Their ability to catalyze the acidolysis reaction between sunflower oil and palmitic and stearic free fatty acids was evaluated in a solvent medium. Effects of modification conditions on the particles hydrophobic character, lipase adsorption and acidolysis activity were investigated. Modified particles were bigger and more hydrophobic than unmodified ones. The most active biocatalyst achieved a change in the composition of palmitic and stearic acid from a value of 9.6% in the original oil to 49.1% in the final structured lipids, representing an almost 3-fold enzyme hyperactivation. This high conversion was maintained during 7 reuse cycles (168 total hours). The results demonstrated that the chitosan modification was effective in order to adsorb and hyperactivate RM lipases.
Fil: Palla, Camila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Fil: Pacheco, Consuelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Fil: Carrin, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Materia
Acidolysis
Adsorption
Chitosan
Reductive Amination
Rhizomucor Miehei Lipase
Structured Lipids
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/56155
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/56155
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Preparation and modification of chitosan particles for Rhizomucor miehei lipase immobilizationPalla, Camila AndreaPacheco, ConsueloCarrin, Maria ElenaAcidolysisAdsorptionChitosanReductive AminationRhizomucor Miehei LipaseStructured Lipidshttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Chitosan particles, suitable as immobilization support, were prepared by precipitation and modified by reductive amination in order to graft linear aliphatic chains of 12 carbon atoms to their native amine groups. Their physical characterization was performed by different techniques: differential scanning calorimetry (DSC), X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), air-water contact angle analysis, among others. Lipases from Rhizomucor miehei (RM) were immobilized by adsorption at low ionic strength onto different modified chitosan microspheres. Their ability to catalyze the acidolysis reaction between sunflower oil and palmitic and stearic free fatty acids was evaluated in a solvent medium. Effects of modification conditions on the particles hydrophobic character, lipase adsorption and acidolysis activity were investigated. Modified particles were bigger and more hydrophobic than unmodified ones. The most active biocatalyst achieved a change in the composition of palmitic and stearic acid from a value of 9.6% in the original oil to 49.1% in the final structured lipids, representing an almost 3-fold enzyme hyperactivation. This high conversion was maintained during 7 reuse cycles (168 total hours). The results demonstrated that the chitosan modification was effective in order to adsorb and hyperactivate RM lipases.Fil: Palla, Camila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; ArgentinaFil: Pacheco, Consuelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; ArgentinaFil: Carrin, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; ArgentinaElsevier Science Sa2011-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/56155Palla, Camila Andrea; Pacheco, Consuelo; Carrin, Maria Elena; Preparation and modification of chitosan particles for Rhizomucor miehei lipase immobilization; Elsevier Science Sa; Biochemical Engineering Journal; 55; 3; 8-2011; 199-2071369-703XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1369703X11001112info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bej.2011.04.008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:29:02Zoai:ri.conicet.gov.ar:11336/56155instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:29:02.96CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Preparation and modification of chitosan particles for Rhizomucor miehei lipase immobilization
title Preparation and modification of chitosan particles for Rhizomucor miehei lipase immobilization
spellingShingle Preparation and modification of chitosan particles for Rhizomucor miehei lipase immobilization
Palla, Camila Andrea
Acidolysis
Adsorption
Chitosan
Reductive Amination
Rhizomucor Miehei Lipase
Structured Lipids
title_short Preparation and modification of chitosan particles for Rhizomucor miehei lipase immobilization
title_full Preparation and modification of chitosan particles for Rhizomucor miehei lipase immobilization
title_fullStr Preparation and modification of chitosan particles for Rhizomucor miehei lipase immobilization
title_full_unstemmed Preparation and modification of chitosan particles for Rhizomucor miehei lipase immobilization
title_sort Preparation and modification of chitosan particles for Rhizomucor miehei lipase immobilization
dc.creator.none.fl_str_mv Palla, Camila Andrea
Pacheco, Consuelo
Carrin, Maria Elena
author Palla, Camila Andrea
author_facet Palla, Camila Andrea
Pacheco, Consuelo
Carrin, Maria Elena
author_role author
author2 Pacheco, Consuelo
Carrin, Maria Elena
author2_role author
author
dc.subject.none.fl_str_mv Acidolysis
Adsorption
Chitosan
Reductive Amination
Rhizomucor Miehei Lipase
Structured Lipids
topic Acidolysis
Adsorption
Chitosan
Reductive Amination
Rhizomucor Miehei Lipase
Structured Lipids
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Chitosan particles, suitable as immobilization support, were prepared by precipitation and modified by reductive amination in order to graft linear aliphatic chains of 12 carbon atoms to their native amine groups. Their physical characterization was performed by different techniques: differential scanning calorimetry (DSC), X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), air-water contact angle analysis, among others. Lipases from Rhizomucor miehei (RM) were immobilized by adsorption at low ionic strength onto different modified chitosan microspheres. Their ability to catalyze the acidolysis reaction between sunflower oil and palmitic and stearic free fatty acids was evaluated in a solvent medium. Effects of modification conditions on the particles hydrophobic character, lipase adsorption and acidolysis activity were investigated. Modified particles were bigger and more hydrophobic than unmodified ones. The most active biocatalyst achieved a change in the composition of palmitic and stearic acid from a value of 9.6% in the original oil to 49.1% in the final structured lipids, representing an almost 3-fold enzyme hyperactivation. This high conversion was maintained during 7 reuse cycles (168 total hours). The results demonstrated that the chitosan modification was effective in order to adsorb and hyperactivate RM lipases.
Fil: Palla, Camila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Fil: Pacheco, Consuelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Fil: Carrin, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
description Chitosan particles, suitable as immobilization support, were prepared by precipitation and modified by reductive amination in order to graft linear aliphatic chains of 12 carbon atoms to their native amine groups. Their physical characterization was performed by different techniques: differential scanning calorimetry (DSC), X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), air-water contact angle analysis, among others. Lipases from Rhizomucor miehei (RM) were immobilized by adsorption at low ionic strength onto different modified chitosan microspheres. Their ability to catalyze the acidolysis reaction between sunflower oil and palmitic and stearic free fatty acids was evaluated in a solvent medium. Effects of modification conditions on the particles hydrophobic character, lipase adsorption and acidolysis activity were investigated. Modified particles were bigger and more hydrophobic than unmodified ones. The most active biocatalyst achieved a change in the composition of palmitic and stearic acid from a value of 9.6% in the original oil to 49.1% in the final structured lipids, representing an almost 3-fold enzyme hyperactivation. This high conversion was maintained during 7 reuse cycles (168 total hours). The results demonstrated that the chitosan modification was effective in order to adsorb and hyperactivate RM lipases.
publishDate 2011
dc.date.none.fl_str_mv 2011-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/56155
Palla, Camila Andrea; Pacheco, Consuelo; Carrin, Maria Elena; Preparation and modification of chitosan particles for Rhizomucor miehei lipase immobilization; Elsevier Science Sa; Biochemical Engineering Journal; 55; 3; 8-2011; 199-207
1369-703X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/56155
identifier_str_mv Palla, Camila Andrea; Pacheco, Consuelo; Carrin, Maria Elena; Preparation and modification of chitosan particles for Rhizomucor miehei lipase immobilization; Elsevier Science Sa; Biochemical Engineering Journal; 55; 3; 8-2011; 199-207
1369-703X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1369703X11001112
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bej.2011.04.008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Sa
publisher.none.fl_str_mv Elsevier Science Sa
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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