Characterization of gastric and neuronal histaminergic populations using a transgenic mouse model

• Autores: Walker, Angela K.; Park, Won Mee; Chuang, Jen Chieh; Perello, Mario; Sakata, Ichiro; Osborne Lawrence, Sherri; Zigman, Jeffrey
• Año de publicación: 2013
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: Transgenic mice; Histidine decarboxylase
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Histamine is a potent biogenic amine that mediates numerous physiological processes throughout the body, including digestion, sleep, and immunity. It is synthesized by gastric enterochromaffin-like cells, a specific set of hypothalamic neurons, as well as a subset of white blood cells, including mast cells. Much remains to be learned about these varied histamine-producing cell populations. Here, we report the validation of a transgenic mouse line in which Cre recombinase expression has been targeted to cells expressing histidine decarboxylase (HDC), which catalyzes the rate-limiting step in the synthesis of histamine. This was achieved by crossing the HDC-Cre mouse line with Rosa26-tdTomato reporter mice, thus resulting in the expression of the fluorescent Tomato (Tmt) signal in cells containing Cre recombinase activity. As expected, the Tmt signal co-localized with HDC-immunoreactivity within the gastric mucosa and gastric submucosa and also within the tuberomamillary nucleus of the brain. HDC expression within Tmt-positive gastric cells was further confirmed by quantitative PCR analysis of mRNA isolated from highly purified populations of Tmt-positive cells obtained by fluorescent activated cell sorting (FACS). HDC expression within these FACS-separated cells was found to coincide with other markers of both ECL cells and mast cells. Gastrin expression was co-localized with HDC expression in a subset of histaminergic gastric mucosal cells. We suggest that these transgenic mice will facilitate future studies aimed at investigating the function of histamine-producing cells.
Fil: Walker, Angela K.. University of Texas; Estados Unidos
Fil: Park, Won Mee. University of Texas; Estados Unidos
Fil: Chuang, Jen Chieh. University of Texas; Estados Unidos
Fil: Perello, Mario. University of Texas. Southwestern Medical Center; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sakata, Ichiro. University of Texas; Estados Unidos
Fil: Osborne Lawrence, Sherri. University of Texas; Estados Unidos
Fil: Zigman, Jeffrey. University of Texas; Estados Unidos