Detection of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?

Autores
Scalise, Maria Lujan; Garimano, Nicolás Ezequiel; Porporato, Melina Mabel; Leonino, Patricia; Pereyra, Adriana; Ferreiros, José A.; Casale, Roberto; Amaral, María Marta; Ibarra, Cristina Adriana; Sacerdoti, Flavia
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Brief Introduction: Some studies have demonstrated that vaginal E. coli colonization may produce complicationsduring pregnancy. We have previously reported that Shiga toxin-2 (Stx2) secreted by enterohemorrhagic E. coli canproduce abortion and premature delivery in animals and can impair human trophoblast in vitro.Objective: The aim of this study was to detect virulence factor genes from Shiga toxin producing E. coli (STEC)in the endocervix of asymptomatic pregnant women. Methods: Endocervical swabs were collected from 103 pregnantwomen (12 to 30 weeks of pregnancy) during their antenatal examination. Swab samples were enriched in TrypticSoy Broth overnight at 37 °C and then streaked into selective and differential medium, Sorbitol MacConkey agar. E.coli detection was confirmed by identification of uidA gene by PCR assay. Then, positive samples for E. coli wereanalyzed for STEC virulence factors genes: stx1, stx2, eae, rfbO157, lpfAO113 and hcpA. After that, positive E. colisamples for stx2 gene were grown in Luria-Bertani Broth medium in order to evaluate Stx2 cytotoxic activity. Forthat, the bacterial supernatants were filter-sterilized and cytotoxicity was evaluated on Vero, Swan 71 and HeLa cells.Results: Our results showed that 14.5% of the endocervical samples were positive for E. coli (positive for uidAgene). Furthermore, 9/15 (60%) of the E. coli-positive samples carried the stx2 gene and 6/15 (40%) carried thelpfAO113 and hcpA genes. One bacterial supernatant from an E. coli-positive endocervical sample carrying stx2 andlpfAO113 genes exhibited high cytotoxic activity on the cells evaluated due to Stx2 production confirmed by specificneutralization of Stx2 with an anti-Stx2 antibody.Conclusions: The novelty of this report is the presence of STEC in the endocervix of asymptomatic pregnantwomen. This opens a new perspective with respect to the possible role of this pathogen in woman reproductivehealth care.
Fil: Scalise, Maria Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Garimano, Nicolás Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Porporato, Melina Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Leonino, Patricia. Hospital Nacional Profesor Alejandro Posadas.; Argentina
Fil: Pereyra, Adriana. Hospital Nacional Profesor Alejandro Posadas.; Argentina
Fil: Ferreiros, José A.. Hospital Nacional Profesor Alejandro Posadas.; Argentina
Fil: Casale, Roberto. Hospital Nacional Profesor Alejandro Posadas.; Argentina
Fil: Amaral, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Sacerdoti, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
International Symposium on Reproductive Health
Virtual Meeting
Singapur
The Physicologycal Society
Biochemical Society
Materia
HUS
Pregnancy
Stx2
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/193057
Acceder
id CONICETDig_f0792c1d959e11954acc3da4beeddfb9
oai_identifier_str oai:ri.conicet.gov.ar:11336/193057
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Detection of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?Scalise, Maria LujanGarimano, Nicolás EzequielPorporato, Melina MabelLeonino, PatriciaPereyra, AdrianaFerreiros, José A.Casale, RobertoAmaral, María MartaIbarra, Cristina AdrianaSacerdoti, FlaviaHUSPregnancyStx2https://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Brief Introduction: Some studies have demonstrated that vaginal E. coli colonization may produce complicationsduring pregnancy. We have previously reported that Shiga toxin-2 (Stx2) secreted by enterohemorrhagic E. coli canproduce abortion and premature delivery in animals and can impair human trophoblast in vitro.Objective: The aim of this study was to detect virulence factor genes from Shiga toxin producing E. coli (STEC)in the endocervix of asymptomatic pregnant women. Methods: Endocervical swabs were collected from 103 pregnantwomen (12 to 30 weeks of pregnancy) during their antenatal examination. Swab samples were enriched in TrypticSoy Broth overnight at 37 °C and then streaked into selective and differential medium, Sorbitol MacConkey agar. E.coli detection was confirmed by identification of uidA gene by PCR assay. Then, positive samples for E. coli wereanalyzed for STEC virulence factors genes: stx1, stx2, eae, rfbO157, lpfAO113 and hcpA. After that, positive E. colisamples for stx2 gene were grown in Luria-Bertani Broth medium in order to evaluate Stx2 cytotoxic activity. Forthat, the bacterial supernatants were filter-sterilized and cytotoxicity was evaluated on Vero, Swan 71 and HeLa cells.Results: Our results showed that 14.5% of the endocervical samples were positive for E. coli (positive for uidAgene). Furthermore, 9/15 (60%) of the E. coli-positive samples carried the stx2 gene and 6/15 (40%) carried thelpfAO113 and hcpA genes. One bacterial supernatant from an E. coli-positive endocervical sample carrying stx2 andlpfAO113 genes exhibited high cytotoxic activity on the cells evaluated due to Stx2 production confirmed by specificneutralization of Stx2 with an anti-Stx2 antibody.Conclusions: The novelty of this report is the presence of STEC in the endocervix of asymptomatic pregnantwomen. This opens a new perspective with respect to the possible role of this pathogen in woman reproductivehealth care.Fil: Scalise, Maria Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Garimano, Nicolás Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Porporato, Melina Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Leonino, Patricia. Hospital Nacional Profesor Alejandro Posadas.; ArgentinaFil: Pereyra, Adriana. Hospital Nacional Profesor Alejandro Posadas.; ArgentinaFil: Ferreiros, José A.. Hospital Nacional Profesor Alejandro Posadas.; ArgentinaFil: Casale, Roberto. Hospital Nacional Profesor Alejandro Posadas.; ArgentinaFil: Amaral, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Sacerdoti, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaInternational Symposium on Reproductive HealthVirtual MeetingSingapurThe Physicologycal SocietyBiochemical SocietyIMR Press2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectSimposioJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/193057Detection of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?; International Symposium on Reproductive Health; Virtual Meeting; Singapur; 2021; 742-7432709-0094CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://imrpress.com/journal/CEOG/48/3/10.31083/j.ceog.2021.03.0511Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:03Zoai:ri.conicet.gov.ar:11336/193057instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:03.516CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?
title Detection of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?
spellingShingle Detection of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?
Scalise, Maria Lujan
HUS
Pregnancy
Stx2
title_short Detection of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?
title_full Detection of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?
title_fullStr Detection of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?
title_full_unstemmed Detection of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?
title_sort Detection of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?
dc.creator.none.fl_str_mv Scalise, Maria Lujan
Garimano, Nicolás Ezequiel
Porporato, Melina Mabel
Leonino, Patricia
Pereyra, Adriana
Ferreiros, José A.
Casale, Roberto
Amaral, María Marta
Ibarra, Cristina Adriana
Sacerdoti, Flavia
author Scalise, Maria Lujan
author_facet Scalise, Maria Lujan
Garimano, Nicolás Ezequiel
Porporato, Melina Mabel
Leonino, Patricia
Pereyra, Adriana
Ferreiros, José A.
Casale, Roberto
Amaral, María Marta
Ibarra, Cristina Adriana
Sacerdoti, Flavia
author_role author
author2 Garimano, Nicolás Ezequiel
Porporato, Melina Mabel
Leonino, Patricia
Pereyra, Adriana
Ferreiros, José A.
Casale, Roberto
Amaral, María Marta
Ibarra, Cristina Adriana
Sacerdoti, Flavia
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv HUS
Pregnancy
Stx2
topic HUS
Pregnancy
Stx2
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Brief Introduction: Some studies have demonstrated that vaginal E. coli colonization may produce complicationsduring pregnancy. We have previously reported that Shiga toxin-2 (Stx2) secreted by enterohemorrhagic E. coli canproduce abortion and premature delivery in animals and can impair human trophoblast in vitro.Objective: The aim of this study was to detect virulence factor genes from Shiga toxin producing E. coli (STEC)in the endocervix of asymptomatic pregnant women. Methods: Endocervical swabs were collected from 103 pregnantwomen (12 to 30 weeks of pregnancy) during their antenatal examination. Swab samples were enriched in TrypticSoy Broth overnight at 37 °C and then streaked into selective and differential medium, Sorbitol MacConkey agar. E.coli detection was confirmed by identification of uidA gene by PCR assay. Then, positive samples for E. coli wereanalyzed for STEC virulence factors genes: stx1, stx2, eae, rfbO157, lpfAO113 and hcpA. After that, positive E. colisamples for stx2 gene were grown in Luria-Bertani Broth medium in order to evaluate Stx2 cytotoxic activity. Forthat, the bacterial supernatants were filter-sterilized and cytotoxicity was evaluated on Vero, Swan 71 and HeLa cells.Results: Our results showed that 14.5% of the endocervical samples were positive for E. coli (positive for uidAgene). Furthermore, 9/15 (60%) of the E. coli-positive samples carried the stx2 gene and 6/15 (40%) carried thelpfAO113 and hcpA genes. One bacterial supernatant from an E. coli-positive endocervical sample carrying stx2 andlpfAO113 genes exhibited high cytotoxic activity on the cells evaluated due to Stx2 production confirmed by specificneutralization of Stx2 with an anti-Stx2 antibody.Conclusions: The novelty of this report is the presence of STEC in the endocervix of asymptomatic pregnantwomen. This opens a new perspective with respect to the possible role of this pathogen in woman reproductivehealth care.
Fil: Scalise, Maria Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Garimano, Nicolás Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Porporato, Melina Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Leonino, Patricia. Hospital Nacional Profesor Alejandro Posadas.; Argentina
Fil: Pereyra, Adriana. Hospital Nacional Profesor Alejandro Posadas.; Argentina
Fil: Ferreiros, José A.. Hospital Nacional Profesor Alejandro Posadas.; Argentina
Fil: Casale, Roberto. Hospital Nacional Profesor Alejandro Posadas.; Argentina
Fil: Amaral, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Sacerdoti, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
International Symposium on Reproductive Health
Virtual Meeting
Singapur
The Physicologycal Society
Biochemical Society
description Brief Introduction: Some studies have demonstrated that vaginal E. coli colonization may produce complicationsduring pregnancy. We have previously reported that Shiga toxin-2 (Stx2) secreted by enterohemorrhagic E. coli canproduce abortion and premature delivery in animals and can impair human trophoblast in vitro.Objective: The aim of this study was to detect virulence factor genes from Shiga toxin producing E. coli (STEC)in the endocervix of asymptomatic pregnant women. Methods: Endocervical swabs were collected from 103 pregnantwomen (12 to 30 weeks of pregnancy) during their antenatal examination. Swab samples were enriched in TrypticSoy Broth overnight at 37 °C and then streaked into selective and differential medium, Sorbitol MacConkey agar. E.coli detection was confirmed by identification of uidA gene by PCR assay. Then, positive samples for E. coli wereanalyzed for STEC virulence factors genes: stx1, stx2, eae, rfbO157, lpfAO113 and hcpA. After that, positive E. colisamples for stx2 gene were grown in Luria-Bertani Broth medium in order to evaluate Stx2 cytotoxic activity. Forthat, the bacterial supernatants were filter-sterilized and cytotoxicity was evaluated on Vero, Swan 71 and HeLa cells.Results: Our results showed that 14.5% of the endocervical samples were positive for E. coli (positive for uidAgene). Furthermore, 9/15 (60%) of the E. coli-positive samples carried the stx2 gene and 6/15 (40%) carried thelpfAO113 and hcpA genes. One bacterial supernatant from an E. coli-positive endocervical sample carrying stx2 andlpfAO113 genes exhibited high cytotoxic activity on the cells evaluated due to Stx2 production confirmed by specificneutralization of Stx2 with an anti-Stx2 antibody.Conclusions: The novelty of this report is the presence of STEC in the endocervix of asymptomatic pregnantwomen. This opens a new perspective with respect to the possible role of this pathogen in woman reproductivehealth care.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Simposio
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/193057
Detection of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?; International Symposium on Reproductive Health; Virtual Meeting; Singapur; 2021; 742-743
2709-0094
CONICET Digital
CONICET
url http://hdl.handle.net/11336/193057
identifier_str_mv Detection of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?; International Symposium on Reproductive Health; Virtual Meeting; Singapur; 2021; 742-743
2709-0094
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://imrpress.com/journal/CEOG/48/3/10.31083/j.ceog.2021.03.0511
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv IMR Press
publisher.none.fl_str_mv IMR Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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