Presence of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?

Autores: Scalise, Maria Lujan; Garimano, Nicolás Ezequiel; Porporato, Melina Mabel; Leonino, P.; Pereyra, A.; Ferreiros, José A.; Casale, Roberto; Amaral, María Marta; Sacerdoti, Flavia; Ibarra, Cristina Adriana
Año de publicación: 2020
Idioma: inglés
Tipo de recurso: documento de conferencia
Estado: versión publicada
Descripción: E. coli can colonize the vagina, usuallyasymptomatically, although epidemiologic studies have showed that the presenceof this bacterium in theendocervix microbiota could be a risk factor for pregnancy. We havepreviously reported that Shiga toxin (Stx) producing E. coli (STEC) infections during pregnancy may cause maternal orfetal damage mediated by Stx2 in rats in early or late stage of gestation. The goal of this study was to detect STEC in the endocervix from asymptomatic pregnant women. Endocervical swabs from 103 asymptomatic pregnant womenwith gestational age of 12 to 30 weeks from the National Hospital Posadas were enrolled.Swab sampleswere enriched in Tryptic Soy Broth andthen streaked on sorbitol-MacConkey (SMAC) agar. E. coli was confirmed by the presence of uidA gene detected by polymerase chainreaction PCR. The positive samples for E.coli were analyzed for STEC virulence factors genes such as: stx1, stx2, eae, rfbO157,lpfAO113 and hcpA genes.The stx2 positive E. coli samples were grown in Luria-BertaniBroth (LB) and the filter-sterilized bacterial supernatants (SN) were usedto evaluated Stx2 activity on Vero, Swan and Hela by cell viability assay. Results: Our results showed that14.6%(15/103) of the endocervical samples were positive for uidA gene. Additionally, we found that8.7% (9/103) was positive for stx2 and 5.8 % (6/103) for lpfAO113 and hcpA genes. The SN of one of themexpressing stx2 gene had a highcytotoxic activity on Vero, Swan 71 and HeLa cells. Stx2 identity was checkedusing an anti-Stx2 antibody in order to neutralize the cytotoxic effects. In conclusion,we demonstrate that STEC can be asymptomatically present in the endocervix andthat can potentially express Stx2. This study may open a new perspective tounderstand whether STEC can be a novel pathogen involved in adverse pregnancyoutcomes.
Fil: Scalise, Maria Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Garimano, Nicolás Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Porporato, Melina Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Leonino, P.. Hospital Nacional Profesor Alejandro Posadas; Argentina
Fil: Pereyra, A.. Hospital Nacional Profesor Alejandro Posadas; Argentina
Fil: Ferreiros, José A.. Hospital Nacional Profesor Alejandro Posadas; Argentina
Fil: Casale, Roberto. Hospital Nacional Profesor Alejandro Posadas; Argentina
Fil: Amaral, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Sacerdoti, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
LXV Reunión anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
Materia: Shiga toxin
Escherichia coli
endocervix
microbiota
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/193265

Acceder

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repository_id_str	3498
network_name_str	CONICET Digital (CONICET)
spelling	Presence of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?Scalise, Maria LujanGarimano, Nicolás EzequielPorporato, Melina MabelLeonino, P.Pereyra, A.Ferreiros, José A.Casale, RobertoAmaral, María MartaSacerdoti, FlaviaIbarra, Cristina AdrianaShiga toxinEscherichia coliendocervixmicrobiotahttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3E. coli can colonize the vagina, usuallyasymptomatically, although epidemiologic studies have showed that the presenceof this bacterium in theendocervix microbiota could be a risk factor for pregnancy. We havepreviously reported that Shiga toxin (Stx) producing E. coli (STEC) infections during pregnancy may cause maternal orfetal damage mediated by Stx2 in rats in early or late stage of gestation. The goal of this study was to detect STEC in the endocervix from asymptomatic pregnant women. Endocervical swabs from 103 asymptomatic pregnant womenwith gestational age of 12 to 30 weeks from the National Hospital Posadas were enrolled.Swab sampleswere enriched in Tryptic Soy Broth andthen streaked on sorbitol-MacConkey (SMAC) agar. E. coli was confirmed by the presence of uidA gene detected by polymerase chainreaction PCR. The positive samples for E.coli were analyzed for STEC virulence factors genes such as: stx1, stx2, eae, rfbO157,lpfAO113 and hcpA genes.The stx2 positive E. coli samples were grown in Luria-BertaniBroth (LB) and the filter-sterilized bacterial supernatants (SN) were usedto evaluated Stx2 activity on Vero, Swan and Hela by cell viability assay. Results: Our results showed that14.6%(15/103) of the endocervical samples were positive for uidA gene. Additionally, we found that8.7% (9/103) was positive for stx2 and 5.8 % (6/103) for lpfAO113 and hcpA genes. The SN of one of themexpressing stx2 gene had a highcytotoxic activity on Vero, Swan 71 and HeLa cells. Stx2 identity was checkedusing an anti-Stx2 antibody in order to neutralize the cytotoxic effects. In conclusion,we demonstrate that STEC can be asymptomatically present in the endocervix andthat can potentially express Stx2. This study may open a new perspective tounderstand whether STEC can be a novel pathogen involved in adverse pregnancyoutcomes.Fil: Scalise, Maria Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Garimano, Nicolás Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Porporato, Melina Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Leonino, P.. Hospital Nacional Profesor Alejandro Posadas; ArgentinaFil: Pereyra, A.. Hospital Nacional Profesor Alejandro Posadas; ArgentinaFil: Ferreiros, José A.. Hospital Nacional Profesor Alejandro Posadas; ArgentinaFil: Casale, Roberto. Hospital Nacional Profesor Alejandro Posadas; ArgentinaFil: Amaral, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Sacerdoti, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaLXV Reunión anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de FisiologíaArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina de FisiologíaFundación Revista Medicina2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/193265Presence of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?; LXV Reunión anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología; Argentina; 2020; 140-1410025-7680CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/reunion-anualNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:14Zoai:ri.conicet.gov.ar:11336/193265instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:14.95CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Presence of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?
title	Presence of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?
spellingShingle	Presence of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes? Scalise, Maria Lujan Shiga toxin Escherichia coli endocervix microbiota
title_short	Presence of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?
title_full	Presence of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?
title_fullStr	Presence of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?
title_full_unstemmed	Presence of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?
title_sort	Presence of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?
dc.creator.none.fl_str_mv	Scalise, Maria Lujan Garimano, Nicolás Ezequiel Porporato, Melina Mabel Leonino, P. Pereyra, A. Ferreiros, José A. Casale, Roberto Amaral, María Marta Sacerdoti, Flavia Ibarra, Cristina Adriana
author	Scalise, Maria Lujan
author_facet	Scalise, Maria Lujan Garimano, Nicolás Ezequiel Porporato, Melina Mabel Leonino, P. Pereyra, A. Ferreiros, José A. Casale, Roberto Amaral, María Marta Sacerdoti, Flavia Ibarra, Cristina Adriana
author_role	author
author2	Garimano, Nicolás Ezequiel Porporato, Melina Mabel Leonino, P. Pereyra, A. Ferreiros, José A. Casale, Roberto Amaral, María Marta Sacerdoti, Flavia Ibarra, Cristina Adriana
author2_role	author author author author author author author author author
dc.subject.none.fl_str_mv	Shiga toxin Escherichia coli endocervix microbiota
topic	Shiga toxin Escherichia coli endocervix microbiota
purl_subject.fl_str_mv	https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv	E. coli can colonize the vagina, usuallyasymptomatically, although epidemiologic studies have showed that the presenceof this bacterium in theendocervix microbiota could be a risk factor for pregnancy. We havepreviously reported that Shiga toxin (Stx) producing E. coli (STEC) infections during pregnancy may cause maternal orfetal damage mediated by Stx2 in rats in early or late stage of gestation. The goal of this study was to detect STEC in the endocervix from asymptomatic pregnant women. Endocervical swabs from 103 asymptomatic pregnant womenwith gestational age of 12 to 30 weeks from the National Hospital Posadas were enrolled.Swab sampleswere enriched in Tryptic Soy Broth andthen streaked on sorbitol-MacConkey (SMAC) agar. E. coli was confirmed by the presence of uidA gene detected by polymerase chainreaction PCR. The positive samples for E.coli were analyzed for STEC virulence factors genes such as: stx1, stx2, eae, rfbO157,lpfAO113 and hcpA genes.The stx2 positive E. coli samples were grown in Luria-BertaniBroth (LB) and the filter-sterilized bacterial supernatants (SN) were usedto evaluated Stx2 activity on Vero, Swan and Hela by cell viability assay. Results: Our results showed that14.6%(15/103) of the endocervical samples were positive for uidA gene. Additionally, we found that8.7% (9/103) was positive for stx2 and 5.8 % (6/103) for lpfAO113 and hcpA genes. The SN of one of themexpressing stx2 gene had a highcytotoxic activity on Vero, Swan 71 and HeLa cells. Stx2 identity was checkedusing an anti-Stx2 antibody in order to neutralize the cytotoxic effects. In conclusion,we demonstrate that STEC can be asymptomatically present in the endocervix andthat can potentially express Stx2. This study may open a new perspective tounderstand whether STEC can be a novel pathogen involved in adverse pregnancyoutcomes. Fil: Scalise, Maria Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina Fil: Garimano, Nicolás Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina Fil: Porporato, Melina Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina Fil: Leonino, P.. Hospital Nacional Profesor Alejandro Posadas; Argentina Fil: Pereyra, A.. Hospital Nacional Profesor Alejandro Posadas; Argentina Fil: Ferreiros, José A.. Hospital Nacional Profesor Alejandro Posadas; Argentina Fil: Casale, Roberto. Hospital Nacional Profesor Alejandro Posadas; Argentina Fil: Amaral, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina Fil: Sacerdoti, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina Fil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina LXV Reunión anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología Argentina Sociedad Argentina de Investigación Clínica Sociedad Argentina de Inmunología Sociedad Argentina de Fisiología
description	E. coli can colonize the vagina, usuallyasymptomatically, although epidemiologic studies have showed that the presenceof this bacterium in theendocervix microbiota could be a risk factor for pregnancy. We havepreviously reported that Shiga toxin (Stx) producing E. coli (STEC) infections during pregnancy may cause maternal orfetal damage mediated by Stx2 in rats in early or late stage of gestation. The goal of this study was to detect STEC in the endocervix from asymptomatic pregnant women. Endocervical swabs from 103 asymptomatic pregnant womenwith gestational age of 12 to 30 weeks from the National Hospital Posadas were enrolled.Swab sampleswere enriched in Tryptic Soy Broth andthen streaked on sorbitol-MacConkey (SMAC) agar. E. coli was confirmed by the presence of uidA gene detected by polymerase chainreaction PCR. The positive samples for E.coli were analyzed for STEC virulence factors genes such as: stx1, stx2, eae, rfbO157,lpfAO113 and hcpA genes.The stx2 positive E. coli samples were grown in Luria-BertaniBroth (LB) and the filter-sterilized bacterial supernatants (SN) were usedto evaluated Stx2 activity on Vero, Swan and Hela by cell viability assay. Results: Our results showed that14.6%(15/103) of the endocervical samples were positive for uidA gene. Additionally, we found that8.7% (9/103) was positive for stx2 and 5.8 % (6/103) for lpfAO113 and hcpA genes. The SN of one of themexpressing stx2 gene had a highcytotoxic activity on Vero, Swan 71 and HeLa cells. Stx2 identity was checkedusing an anti-Stx2 antibody in order to neutralize the cytotoxic effects. In conclusion,we demonstrate that STEC can be asymptomatically present in the endocervix andthat can potentially express Stx2. This study may open a new perspective tounderstand whether STEC can be a novel pathogen involved in adverse pregnancyoutcomes.
publishDate	2020
dc.date.none.fl_str_mv	2020
dc.type.none.fl_str_mv	info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia
status_str	publishedVersion
format	conferenceObject
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/193265 Presence of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?; LXV Reunión anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología; Argentina; 2020; 140-141 0025-7680 CONICET Digital CONICET
url	http://hdl.handle.net/11336/193265
identifier_str_mv	Presence of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?; LXV Reunión anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología; Argentina; 2020; 140-141 0025-7680 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
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dc.publisher.none.fl_str_mv	Fundación Revista Medicina
publisher.none.fl_str_mv	Fundación Revista Medicina
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Presence of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?

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