Metabolomic profiling of the halophyte Prosopis strombulifera shows sodium salt- specific response

Autores
Llanes, Analia Susana; Arbona, Vicent; Gómez Cadenas, Aurelio; Luna, Virginia
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Primary and secondary metabolite profiles were analyzed in roots and leaves of the halophytic shrub Prosopis strombulifera in response to control plants (no salt added in the growing media) and to lowering the osmotic potential to −1.0, −1.9, and −2.6 MPa generated by NaCl, Na2SO4, and the iso-osmotic combination of them at 24 h after reaching such potential. A rapid production of metabolites in response to sodium salt was found, which was correlated with modifications in growth parameters. Analysis of polar metabolite profiles by GC-MS rendered a total of 108 significantly altered compounds including 18 amino acids, 19 secondary metabolites, 23 carbohydrates, 13 organic acids, 4 indole acids, among others. Primary metabolites showed a differential response under the salt treatments, which was dependent on salt type and concentration, organ and age of plants. Most of identified compounds showed the strongest accumulation at the highest salt concentration assayed for Na2SO4-treated plants, which was correlated with damaging effects of sulfate anion on plant growth. Roots of NaCl-treated plants showed a higher number of altered metabolites (analyzed by UPLC-ESI-QqTOF-MS) compared to other treatments, while leaves of Na2SO4-treated plants showed the highest number of altered signals. A low degree of overlapping between secondary metabolites altered in roots and leaves of NaCl and Na2SO4-treated plants was found. However, when both NaCl and Na2SO4 salts were present plants always showed a lower number of altered metabolites. Three compounds were tentatively identified: tryptophan, lysophosphatidylcoline and 13-hydroxyoctadecadienoic acid. Increasing knowledge on P. strombulifera metabolism will contribute to unravel the underlying biochemical mechanism of salt tolerance.
Fil: Llanes, Analia Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Arbona, Vicent. Universitat Jaume I. Escuela Superior de Tecnología y Cs. Experimentales; España
Fil: Gómez Cadenas, Aurelio. Universitat Jaume I. Escuela Superior de Tecnología y Cs. Experimentales; España
Fil: Luna, Virginia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Ciencias Naturales; Argentina
Materia
GAS CHROMATOGRAPHY-MASS SPECTROMETRY
HALOPHYTIC WOODY SPECIES
LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY
METABOLOMIC PROFILING
NA2SO4
NACL
SALT TOLERANCE MECHANISMS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/179978
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/179978
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Metabolomic profiling of the halophyte Prosopis strombulifera shows sodium salt- specific responseLlanes, Analia SusanaArbona, VicentGómez Cadenas, AurelioLuna, VirginiaGAS CHROMATOGRAPHY-MASS SPECTROMETRYHALOPHYTIC WOODY SPECIESLIQUID CHROMATOGRAPHY-MASS SPECTROMETRYMETABOLOMIC PROFILINGNA2SO4NACLSALT TOLERANCE MECHANISMShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Primary and secondary metabolite profiles were analyzed in roots and leaves of the halophytic shrub Prosopis strombulifera in response to control plants (no salt added in the growing media) and to lowering the osmotic potential to −1.0, −1.9, and −2.6 MPa generated by NaCl, Na2SO4, and the iso-osmotic combination of them at 24 h after reaching such potential. A rapid production of metabolites in response to sodium salt was found, which was correlated with modifications in growth parameters. Analysis of polar metabolite profiles by GC-MS rendered a total of 108 significantly altered compounds including 18 amino acids, 19 secondary metabolites, 23 carbohydrates, 13 organic acids, 4 indole acids, among others. Primary metabolites showed a differential response under the salt treatments, which was dependent on salt type and concentration, organ and age of plants. Most of identified compounds showed the strongest accumulation at the highest salt concentration assayed for Na2SO4-treated plants, which was correlated with damaging effects of sulfate anion on plant growth. Roots of NaCl-treated plants showed a higher number of altered metabolites (analyzed by UPLC-ESI-QqTOF-MS) compared to other treatments, while leaves of Na2SO4-treated plants showed the highest number of altered signals. A low degree of overlapping between secondary metabolites altered in roots and leaves of NaCl and Na2SO4-treated plants was found. However, when both NaCl and Na2SO4 salts were present plants always showed a lower number of altered metabolites. Three compounds were tentatively identified: tryptophan, lysophosphatidylcoline and 13-hydroxyoctadecadienoic acid. Increasing knowledge on P. strombulifera metabolism will contribute to unravel the underlying biochemical mechanism of salt tolerance.Fil: Llanes, Analia Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Arbona, Vicent. Universitat Jaume I. Escuela Superior de Tecnología y Cs. Experimentales; EspañaFil: Gómez Cadenas, Aurelio. Universitat Jaume I. Escuela Superior de Tecnología y Cs. Experimentales; EspañaFil: Luna, Virginia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Ciencias Naturales; ArgentinaElsevier France-Editions Scientifiques Medicales Elsevier2016-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/179978Llanes, Analia Susana; Arbona, Vicent; Gómez Cadenas, Aurelio; Luna, Virginia; Metabolomic profiling of the halophyte Prosopis strombulifera shows sodium salt- specific response; Elsevier France-Editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 108; 7-2016; 145-1570981-9428CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.plaphy.2016.07.010info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:33:28Zoai:ri.conicet.gov.ar:11336/179978instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:33:28.852CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Metabolomic profiling of the halophyte Prosopis strombulifera shows sodium salt- specific response
title Metabolomic profiling of the halophyte Prosopis strombulifera shows sodium salt- specific response
spellingShingle Metabolomic profiling of the halophyte Prosopis strombulifera shows sodium salt- specific response
Llanes, Analia Susana
GAS CHROMATOGRAPHY-MASS SPECTROMETRY
HALOPHYTIC WOODY SPECIES
LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY
METABOLOMIC PROFILING
NA2SO4
NACL
SALT TOLERANCE MECHANISMS
title_short Metabolomic profiling of the halophyte Prosopis strombulifera shows sodium salt- specific response
title_full Metabolomic profiling of the halophyte Prosopis strombulifera shows sodium salt- specific response
title_fullStr Metabolomic profiling of the halophyte Prosopis strombulifera shows sodium salt- specific response
title_full_unstemmed Metabolomic profiling of the halophyte Prosopis strombulifera shows sodium salt- specific response
title_sort Metabolomic profiling of the halophyte Prosopis strombulifera shows sodium salt- specific response
dc.creator.none.fl_str_mv Llanes, Analia Susana
Arbona, Vicent
Gómez Cadenas, Aurelio
Luna, Virginia
author Llanes, Analia Susana
author_facet Llanes, Analia Susana
Arbona, Vicent
Gómez Cadenas, Aurelio
Luna, Virginia
author_role author
author2 Arbona, Vicent
Gómez Cadenas, Aurelio
Luna, Virginia
author2_role author
author
author
dc.subject.none.fl_str_mv GAS CHROMATOGRAPHY-MASS SPECTROMETRY
HALOPHYTIC WOODY SPECIES
LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY
METABOLOMIC PROFILING
NA2SO4
NACL
SALT TOLERANCE MECHANISMS
topic GAS CHROMATOGRAPHY-MASS SPECTROMETRY
HALOPHYTIC WOODY SPECIES
LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY
METABOLOMIC PROFILING
NA2SO4
NACL
SALT TOLERANCE MECHANISMS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Primary and secondary metabolite profiles were analyzed in roots and leaves of the halophytic shrub Prosopis strombulifera in response to control plants (no salt added in the growing media) and to lowering the osmotic potential to −1.0, −1.9, and −2.6 MPa generated by NaCl, Na2SO4, and the iso-osmotic combination of them at 24 h after reaching such potential. A rapid production of metabolites in response to sodium salt was found, which was correlated with modifications in growth parameters. Analysis of polar metabolite profiles by GC-MS rendered a total of 108 significantly altered compounds including 18 amino acids, 19 secondary metabolites, 23 carbohydrates, 13 organic acids, 4 indole acids, among others. Primary metabolites showed a differential response under the salt treatments, which was dependent on salt type and concentration, organ and age of plants. Most of identified compounds showed the strongest accumulation at the highest salt concentration assayed for Na2SO4-treated plants, which was correlated with damaging effects of sulfate anion on plant growth. Roots of NaCl-treated plants showed a higher number of altered metabolites (analyzed by UPLC-ESI-QqTOF-MS) compared to other treatments, while leaves of Na2SO4-treated plants showed the highest number of altered signals. A low degree of overlapping between secondary metabolites altered in roots and leaves of NaCl and Na2SO4-treated plants was found. However, when both NaCl and Na2SO4 salts were present plants always showed a lower number of altered metabolites. Three compounds were tentatively identified: tryptophan, lysophosphatidylcoline and 13-hydroxyoctadecadienoic acid. Increasing knowledge on P. strombulifera metabolism will contribute to unravel the underlying biochemical mechanism of salt tolerance.
Fil: Llanes, Analia Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Arbona, Vicent. Universitat Jaume I. Escuela Superior de Tecnología y Cs. Experimentales; España
Fil: Gómez Cadenas, Aurelio. Universitat Jaume I. Escuela Superior de Tecnología y Cs. Experimentales; España
Fil: Luna, Virginia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Ciencias Naturales; Argentina
description Primary and secondary metabolite profiles were analyzed in roots and leaves of the halophytic shrub Prosopis strombulifera in response to control plants (no salt added in the growing media) and to lowering the osmotic potential to −1.0, −1.9, and −2.6 MPa generated by NaCl, Na2SO4, and the iso-osmotic combination of them at 24 h after reaching such potential. A rapid production of metabolites in response to sodium salt was found, which was correlated with modifications in growth parameters. Analysis of polar metabolite profiles by GC-MS rendered a total of 108 significantly altered compounds including 18 amino acids, 19 secondary metabolites, 23 carbohydrates, 13 organic acids, 4 indole acids, among others. Primary metabolites showed a differential response under the salt treatments, which was dependent on salt type and concentration, organ and age of plants. Most of identified compounds showed the strongest accumulation at the highest salt concentration assayed for Na2SO4-treated plants, which was correlated with damaging effects of sulfate anion on plant growth. Roots of NaCl-treated plants showed a higher number of altered metabolites (analyzed by UPLC-ESI-QqTOF-MS) compared to other treatments, while leaves of Na2SO4-treated plants showed the highest number of altered signals. A low degree of overlapping between secondary metabolites altered in roots and leaves of NaCl and Na2SO4-treated plants was found. However, when both NaCl and Na2SO4 salts were present plants always showed a lower number of altered metabolites. Three compounds were tentatively identified: tryptophan, lysophosphatidylcoline and 13-hydroxyoctadecadienoic acid. Increasing knowledge on P. strombulifera metabolism will contribute to unravel the underlying biochemical mechanism of salt tolerance.
publishDate 2016
dc.date.none.fl_str_mv 2016-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/179978
Llanes, Analia Susana; Arbona, Vicent; Gómez Cadenas, Aurelio; Luna, Virginia; Metabolomic profiling of the halophyte Prosopis strombulifera shows sodium salt- specific response; Elsevier France-Editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 108; 7-2016; 145-157
0981-9428
CONICET Digital
CONICET
url http://hdl.handle.net/11336/179978
identifier_str_mv Llanes, Analia Susana; Arbona, Vicent; Gómez Cadenas, Aurelio; Luna, Virginia; Metabolomic profiling of the halophyte Prosopis strombulifera shows sodium salt- specific response; Elsevier France-Editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 108; 7-2016; 145-157
0981-9428
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plaphy.2016.07.010
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier France-Editions Scientifiques Medicales Elsevier
publisher.none.fl_str_mv Elsevier France-Editions Scientifiques Medicales Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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